Stackebrandtia albiflava

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Glycomycetales; Glycomycetaceae; Stackebrandtia

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5212 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A562UQ83|A0A562UQ83_9ACTN Peptide/nickel transport system ATP-binding protein/oligopeptide transport system ATP-binding protein OS=Stackebrandtia albiflava OX=406432 GN=LX16_4916 PE=4 SV=1
MM1 pKa = 6.86TRR3 pKa = 11.84IRR5 pKa = 11.84RR6 pKa = 11.84LSYY9 pKa = 10.26IAMAAALSFTAAACTSQGPSNDD31 pKa = 3.33AQNQTEE37 pKa = 4.33TQDD40 pKa = 3.4VVAGGEE46 pKa = 3.86ITIAATSPITDD57 pKa = 3.76WNPISANGDD66 pKa = 3.33TTGQRR71 pKa = 11.84QQQWPLYY78 pKa = 7.17PHH80 pKa = 7.44PFLTLPDD87 pKa = 3.53TSVVINEE94 pKa = 4.29ALLEE98 pKa = 4.26KK99 pKa = 10.58ADD101 pKa = 3.93VVEE104 pKa = 4.23TDD106 pKa = 3.26PMTVEE111 pKa = 4.59YY112 pKa = 10.66VLQDD116 pKa = 3.27GVQWSDD122 pKa = 3.19GTPIGVDD129 pKa = 2.98DD130 pKa = 4.63FVYY133 pKa = 9.37TQAVQDD139 pKa = 3.92PSKK142 pKa = 10.85CAEE145 pKa = 4.06CLAAFTEE152 pKa = 5.37GYY154 pKa = 10.67SDD156 pKa = 3.18ITSIEE161 pKa = 3.73ASEE164 pKa = 4.42DD165 pKa = 3.22GKK167 pKa = 9.59TITMVYY173 pKa = 10.07SEE175 pKa = 5.32PFSEE179 pKa = 3.8WRR181 pKa = 11.84ALFNYY186 pKa = 9.65ILPAHH191 pKa = 5.61VAEE194 pKa = 5.52GYY196 pKa = 11.16GDD198 pKa = 4.16LATSFNEE205 pKa = 4.25GFSKK209 pKa = 10.63NVPEE213 pKa = 4.55FSGGPYY219 pKa = 9.86IIQDD223 pKa = 3.69YY224 pKa = 10.11TDD226 pKa = 5.2GISMTMVPNPNWYY239 pKa = 10.49GEE241 pKa = 4.25GPNLEE246 pKa = 4.75TITTRR251 pKa = 11.84YY252 pKa = 8.0ITGQGEE258 pKa = 4.29QLTALQSGEE267 pKa = 4.15VQLVYY272 pKa = 9.89ATPTVDD278 pKa = 3.37TMDD281 pKa = 4.14QARR284 pKa = 11.84QLPNMTINTGSTLTYY299 pKa = 9.44YY300 pKa = 10.66HH301 pKa = 7.21LGMKK305 pKa = 7.36TTGDD309 pKa = 3.77VMSDD313 pKa = 3.19PALRR317 pKa = 11.84SAIATALDD325 pKa = 3.88LGDD328 pKa = 3.45MRR330 pKa = 11.84EE331 pKa = 3.84RR332 pKa = 11.84TIGQFAPDD340 pKa = 3.86VPQMKK345 pKa = 9.97SSVYY349 pKa = 10.59VPGQQIGGQEE359 pKa = 3.98AYY361 pKa = 9.96RR362 pKa = 11.84DD363 pKa = 3.58NMTDD367 pKa = 3.35LGIGAGDD374 pKa = 3.45VEE376 pKa = 4.58GAIGILEE383 pKa = 4.27DD384 pKa = 3.53AGYY387 pKa = 8.98TITDD391 pKa = 3.6EE392 pKa = 5.36QLIQPDD398 pKa = 4.19GTPLRR403 pKa = 11.84DD404 pKa = 3.84LSFLTLGTDD413 pKa = 3.62VLRR416 pKa = 11.84MEE418 pKa = 4.84VAQIAQQQLLALGITINIDD437 pKa = 3.14AADD440 pKa = 4.05GARR443 pKa = 11.84YY444 pKa = 9.83SPALRR449 pKa = 11.84EE450 pKa = 3.94GDD452 pKa = 3.92FDD454 pKa = 5.65LMATGTALDD463 pKa = 4.75LGPLSMQQWYY473 pKa = 8.49GTDD476 pKa = 3.32APRR479 pKa = 11.84SFGYY483 pKa = 10.69SNPEE487 pKa = 3.25ADD489 pKa = 4.59ALLEE493 pKa = 4.25AAASEE498 pKa = 4.69LDD500 pKa = 3.37PAAQIEE506 pKa = 4.63LMNEE510 pKa = 4.16LDD512 pKa = 4.57RR513 pKa = 11.84VLLGDD518 pKa = 4.01GVVLPLFGTPMMAVYY533 pKa = 10.17PNTYY537 pKa = 10.52GNIFINPSKK546 pKa = 10.97YY547 pKa = 8.03GTTMNVEE554 pKa = 3.93EE555 pKa = 4.68WGLLAEE561 pKa = 5.34PGTSS565 pKa = 3.06

Molecular weight: 60.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A562V129|A0A562V129_9ACTN DNA replication and repair protein RecF OS=Stackebrandtia albiflava OX=406432 GN=recF PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AIVAARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84GRR40 pKa = 11.84ARR42 pKa = 11.84LTAA45 pKa = 4.29

Molecular weight: 5.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5212

0

5212

1617362

37

3801

310.3

33.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.179 ± 0.049

0.807 ± 0.01

6.61 ± 0.034

5.673 ± 0.033

2.826 ± 0.018

9.155 ± 0.035

2.324 ± 0.019

3.456 ± 0.021

1.6 ± 0.02

9.98 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.965 ± 0.015

1.717 ± 0.015

6.047 ± 0.035

2.348 ± 0.02

8.336 ± 0.042

4.928 ± 0.022

6.369 ± 0.031

9.011 ± 0.036

1.612 ± 0.014

2.059 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski