Legionella quinlivanii
Average proteome isoelectric point is 6.56
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2989 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0W0XY50|A0A0W0XY50_9GAMM Hemolysin D OS=Legionella quinlivanii OX=45073 GN=Lqui_1914 PE=4 SV=1
MM1 pKa = 7.35 AVAVNSGIFSQLALNTQFEE20 pKa = 4.77 LSQVTRR26 pKa = 11.84 ILPLFLSNTLVVSINNSFFSVQRR49 pKa = 11.84 PVYY52 pKa = 9.46 PPADD56 pKa = 3.23 VAEE59 pKa = 4.31 RR60 pKa = 11.84 DD61 pKa = 3.79 EE62 pKa = 4.53 GVKK65 pKa = 10.22 QADD68 pKa = 3.4 LDD70 pKa = 4.1 LFNYY74 pKa = 9.91 LLNFLPPSYY83 pKa = 10.65 LIVEE87 pKa = 4.63 PDD89 pKa = 3.46 NLDD92 pKa = 3.3 GNTFVSFNPYY102 pKa = 9.94 YY103 pKa = 10.78 SLGYY107 pKa = 10.14 VDD109 pKa = 5.63 AGYY112 pKa = 7.94 DD113 pKa = 3.33 TLGISQSIINTLLDD127 pKa = 2.98 NWFFLSRR134 pKa = 11.84 RR135 pKa = 11.84 LTLLNSDD142 pKa = 3.41 ADD144 pKa = 4.23 EE145 pKa = 4.71 NNSEE149 pKa = 4.14 SSNEE153 pKa = 3.81 SRR155 pKa = 11.84 EE156 pKa = 4.25 SNASVNVSLFEE167 pKa = 4.66 SGLQNGHH174 pKa = 6.78 APGAATAYY182 pKa = 10.31 SGNLSLLNPEE192 pKa = 4.39 NSPVALAAGSGVLPFTSNDD211 pKa = 2.69 IPINYY216 pKa = 9.09 IIEE219 pKa = 4.21 NSQLIAQAGGEE230 pKa = 4.52 TIFILQILPNNQFSFQLNNNLDD252 pKa = 3.82 HH253 pKa = 7.32 PLPGADD259 pKa = 4.18 QIVIDD264 pKa = 4.76 LSEE267 pKa = 5.11 FIQIGNINLPPGTFIIRR284 pKa = 11.84 INDD287 pKa = 4.68 DD288 pKa = 3.01 IPLAVAGSNQQSLLHH303 pKa = 6.62 EE304 pKa = 4.82 DD305 pKa = 4.06 ALPGANIDD313 pKa = 3.89 TPNPLSEE320 pKa = 3.98 ISGALSALVSFGADD334 pKa = 3.19 GQAGFHH340 pKa = 6.98 FISEE344 pKa = 4.34 PAISTVLEE352 pKa = 4.23 SFGLSSGGQPLDD364 pKa = 3.42 YY365 pKa = 10.56 QVADD369 pKa = 3.72 NRR371 pKa = 11.84 LQGLDD376 pKa = 3.42 PDD378 pKa = 4.25 GNEE381 pKa = 3.77 VFTLIVNTDD390 pKa = 2.73 GSYY393 pKa = 10.66 QFILQQSLDD402 pKa = 3.89 HH403 pKa = 6.66 LGSNASTILFPWVFF417 pKa = 3.42
Molecular weight: 44.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.691
IPC2_protein 3.719
IPC_protein 3.732
Toseland 3.516
ProMoST 3.897
Dawson 3.719
Bjellqvist 3.884
Wikipedia 3.656
Rodwell 3.554
Grimsley 3.427
Solomon 3.719
Lehninger 3.668
Nozaki 3.834
DTASelect 4.075
Thurlkill 3.567
EMBOSS 3.668
Sillero 3.859
Patrickios 1.163
IPC_peptide 3.719
IPC2_peptide 3.834
IPC2.peptide.svr19 3.758
Protein with the highest isoelectric point:
>tr|A0A0W0Y1C9|A0A0W0Y1C9_9GAMM Phosphopentomutase OS=Legionella quinlivanii OX=45073 GN=deoB PE=3 SV=1
MM1 pKa = 7.63 ISRR4 pKa = 11.84 SVYY7 pKa = 9.23 HH8 pKa = 5.04 YY9 pKa = 10.22 RR10 pKa = 11.84 SRR12 pKa = 11.84 RR13 pKa = 11.84 GTDD16 pKa = 2.52 AVVRR20 pKa = 11.84 HH21 pKa = 6.87 RR22 pKa = 11.84 IRR24 pKa = 11.84 EE25 pKa = 3.85 IAEE28 pKa = 3.67 TRR30 pKa = 11.84 VRR32 pKa = 11.84 YY33 pKa = 9.44 GYY35 pKa = 10.32 QRR37 pKa = 11.84 IHH39 pKa = 6.68 VLLQRR44 pKa = 11.84 EE45 pKa = 4.41 GWIINHH51 pKa = 6.28 KK52 pKa = 9.3 KK53 pKa = 7.58 VHH55 pKa = 6.85 RR56 pKa = 11.84 IYY58 pKa = 11.03 CEE60 pKa = 3.35 EE61 pKa = 3.94 GLNLRR66 pKa = 11.84 RR67 pKa = 11.84 KK68 pKa = 9.2 RR69 pKa = 11.84 PRR71 pKa = 11.84 RR72 pKa = 11.84 NVAGAHH78 pKa = 5.91 RR79 pKa = 11.84 EE80 pKa = 4.02 DD81 pKa = 4.36 RR82 pKa = 11.84 SLASALDD89 pKa = 4.08 EE90 pKa = 4.4 CWSMDD95 pKa = 3.66 FVSDD99 pKa = 3.62 SLFNGRR105 pKa = 11.84 RR106 pKa = 11.84 IRR108 pKa = 11.84 ALTIVDD114 pKa = 3.57 NFSRR118 pKa = 11.84 EE119 pKa = 3.93 CLAIHH124 pKa = 6.5 VGQAIRR130 pKa = 11.84 GEE132 pKa = 4.12 DD133 pKa = 3.59 VACVLEE139 pKa = 4.23 GLRR142 pKa = 11.84 VMEE145 pKa = 5.03 NSGQKK150 pKa = 9.98 AA151 pKa = 3.05
Molecular weight: 17.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.334
IPC2_protein 9.443
IPC_protein 10.409
Toseland 10.248
ProMoST 10.116
Dawson 10.452
Bjellqvist 10.248
Wikipedia 10.716
Rodwell 10.467
Grimsley 10.54
Solomon 10.555
Lehninger 10.511
Nozaki 10.321
DTASelect 10.233
Thurlkill 10.321
EMBOSS 10.672
Sillero 10.394
Patrickios 10.058
IPC_peptide 10.555
IPC2_peptide 9.575
IPC2.peptide.svr19 8.457
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2989
0
2989
1064207
50
4606
356.0
39.94
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.982 ± 0.049
1.173 ± 0.019
4.901 ± 0.03
6.247 ± 0.043
4.448 ± 0.035
6.058 ± 0.047
2.375 ± 0.021
7.018 ± 0.037
5.798 ± 0.043
11.193 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.309 ± 0.021
4.826 ± 0.038
4.171 ± 0.025
4.812 ± 0.039
4.607 ± 0.039
6.921 ± 0.035
4.888 ± 0.032
5.78 ± 0.041
1.128 ± 0.015
3.358 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here