Lonchura striata domestica (Bengalese finch)
Average proteome isoelectric point is 6.65
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 15252 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A218UU03|A0A218UU03_9PASE P-type Cu(+) transporter OS=Lonchura striata domestica OX=299123 GN=ATP7A PE=4 SV=1
MM1 pKa = 7.61 RR2 pKa = 11.84 SVLVFVCLVGMASTFSVKK20 pKa = 9.52 SWLLRR25 pKa = 11.84 PKK27 pKa = 10.68 LDD29 pKa = 3.98 DD30 pKa = 3.79 SEE32 pKa = 4.53 EE33 pKa = 3.99 NAVFKK38 pKa = 10.53 RR39 pKa = 11.84 QHH41 pKa = 4.39 RR42 pKa = 11.84 HH43 pKa = 3.81 YY44 pKa = 9.3 LYY46 pKa = 10.51 KK47 pKa = 10.56 YY48 pKa = 8.22 IYY50 pKa = 9.72 VYY52 pKa = 10.48 SPQRR56 pKa = 11.84 RR57 pKa = 11.84 YY58 pKa = 10.02 QGSDD62 pKa = 2.81 SSEE65 pKa = 4.14 EE66 pKa = 4.0 EE67 pKa = 4.01 GDD69 pKa = 3.57 GSEE72 pKa = 4.25 EE73 pKa = 4.12 EE74 pKa = 4.51 EE75 pKa = 4.24 EE76 pKa = 4.67 GEE78 pKa = 4.03 PFYY81 pKa = 11.23 AGTKK85 pKa = 9.38 AAGHH89 pKa = 6.35 PAGVAPEE96 pKa = 4.36 DD97 pKa = 3.85 CQGALTGDD105 pKa = 3.58 VTSPQQSEE113 pKa = 4.56 GCQRR117 pKa = 11.84 IRR119 pKa = 11.84 QRR121 pKa = 11.84 TANKK125 pKa = 10.43 EE126 pKa = 3.79 EE127 pKa = 4.36 DD128 pKa = 3.99 SEE130 pKa = 5.68 NEE132 pKa = 3.81 DD133 pKa = 3.02 SDD135 pKa = 4.01 EE136 pKa = 4.45 NEE138 pKa = 4.03 EE139 pKa = 4.09 EE140 pKa = 4.38 EE141 pKa = 4.16 EE142 pKa = 4.63 DD143 pKa = 4.2 EE144 pKa = 5.17 EE145 pKa = 5.23 VDD147 pKa = 3.79 EE148 pKa = 5.51 NEE150 pKa = 4.03 NGVNGTSTNTTEE162 pKa = 5.28 GIGPHH167 pKa = 6.52 GNGTVAAEE175 pKa = 4.12 EE176 pKa = 4.15 ATGEE180 pKa = 4.08 AEE182 pKa = 3.98 EE183 pKa = 4.38 EE184 pKa = 4.32 EE185 pKa = 4.29 EE186 pKa = 4.57 GEE188 pKa = 4.12 EE189 pKa = 4.08 EE190 pKa = 4.15 EE191 pKa = 4.44 EE192 pKa = 4.19 EE193 pKa = 4.23 EE194 pKa = 4.21 EE195 pKa = 4.21 EE196 pKa = 4.21 EE197 pKa = 4.21 EE198 pKa = 4.38 EE199 pKa = 4.31 EE200 pKa = 4.29 EE201 pKa = 4.28 EE202 pKa = 4.34 EE203 pKa = 4.05 EE204 pKa = 5.47 AEE206 pKa = 4.16 ATTIASTTNEE216 pKa = 4.08 EE217 pKa = 4.57 GLTQATTIDD226 pKa = 3.9 DD227 pKa = 4.58 GGPTDD232 pKa = 3.8 ATTDD236 pKa = 3.17 ATTVGEE242 pKa = 3.97 LWEE245 pKa = 4.28 YY246 pKa = 11.32 DD247 pKa = 3.46 VTARR251 pKa = 11.84 DD252 pKa = 3.47 HH253 pKa = 6.85 SRR255 pKa = 11.84 GEE257 pKa = 3.96 EE258 pKa = 4.19 GTTEE262 pKa = 4.32 GGYY265 pKa = 10.63 EE266 pKa = 4.11 DD267 pKa = 3.39 QDD269 pKa = 3.33 EE270 pKa = 4.43 YY271 pKa = 11.94 ARR273 pKa = 11.84 GDD275 pKa = 3.38 SYY277 pKa = 11.26 RR278 pKa = 11.84 AYY280 pKa = 9.36 EE281 pKa = 4.68 DD282 pKa = 3.56 EE283 pKa = 3.82 YY284 pKa = 11.36 GYY286 pKa = 11.4 YY287 pKa = 9.75 KK288 pKa = 10.73 GHH290 pKa = 6.85 GYY292 pKa = 10.44 DD293 pKa = 4.46 VYY295 pKa = 11.29 GQDD298 pKa = 3.68 YY299 pKa = 10.48 YY300 pKa = 11.86 YY301 pKa = 11.11 SQQ303 pKa = 3.52
Molecular weight: 33.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.758
IPC2_protein 3.91
IPC_protein 3.872
Toseland 3.706
ProMoST 3.973
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.656
Rodwell 3.706
Grimsley 3.617
Solomon 3.808
Lehninger 3.757
Nozaki 3.91
DTASelect 4.024
Thurlkill 3.706
EMBOSS 3.681
Sillero 3.973
Patrickios 1.113
IPC_peptide 3.808
IPC2_peptide 3.961
IPC2.peptide.svr19 3.86
Protein with the highest isoelectric point:
>tr|A0A218UBT3|A0A218UBT3_9PASE Inhibitor of growth protein OS=Lonchura striata domestica OX=299123 GN=ING5 PE=3 SV=1
MM1 pKa = 7.28 WRR3 pKa = 11.84 VALPRR8 pKa = 11.84 WLPRR12 pKa = 11.84 ARR14 pKa = 11.84 RR15 pKa = 11.84 GLGQHH20 pKa = 6.39 RR21 pKa = 11.84 PHH23 pKa = 6.54 LQLSPAAGQLGRR35 pKa = 11.84 AGGRR39 pKa = 11.84 LSQAAPLRR47 pKa = 11.84 RR48 pKa = 11.84 GAAGGAGAAA57 pKa = 4.23
Molecular weight: 5.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.513
IPC2_protein 11.184
IPC_protein 12.793
Toseland 12.954
ProMoST 13.451
Dawson 12.954
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.457
Grimsley 12.998
Solomon 13.451
Lehninger 13.349
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.451
Sillero 12.954
Patrickios 12.193
IPC_peptide 13.451
IPC2_peptide 12.442
IPC2.peptide.svr19 9.197
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
15247
5
15252
8504911
8
16936
557.6
62.27
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.093 ± 0.02
2.239 ± 0.016
4.937 ± 0.014
7.289 ± 0.025
3.675 ± 0.014
6.294 ± 0.022
2.528 ± 0.01
4.523 ± 0.014
6.001 ± 0.026
9.793 ± 0.027
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.24 ± 0.01
3.789 ± 0.015
5.791 ± 0.026
4.751 ± 0.02
5.52 ± 0.019
8.27 ± 0.026
5.192 ± 0.016
6.129 ± 0.018
1.193 ± 0.006
2.749 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here