Clostridium frigidicarnis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3915 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I0WX67|A0A1I0WX67_9CLOT HDIG domain-containing protein OS=Clostridium frigidicarnis OX=84698 GN=SAMN04488528_1006130 PE=4 SV=1
MM1 pKa = 8.06DD2 pKa = 4.51IKK4 pKa = 11.17NVLEE8 pKa = 3.95YY9 pKa = 10.7LGKK12 pKa = 10.16NDD14 pKa = 3.61LTDD17 pKa = 3.35IEE19 pKa = 5.06EE20 pKa = 4.2INEE23 pKa = 3.84KK24 pKa = 10.54DD25 pKa = 3.1GCKK28 pKa = 10.05VIRR31 pKa = 11.84FFYY34 pKa = 10.93DD35 pKa = 3.19FDD37 pKa = 4.54KK38 pKa = 11.71EE39 pKa = 3.99EE40 pKa = 4.02MEE42 pKa = 4.42AARR45 pKa = 11.84AYY47 pKa = 11.06SKK49 pKa = 10.87DD50 pKa = 3.24EE51 pKa = 4.3CEE53 pKa = 4.16EE54 pKa = 4.27EE55 pKa = 4.37EE56 pKa = 6.05NSDD59 pKa = 2.98TWYY62 pKa = 10.55DD63 pKa = 3.9EE64 pKa = 4.39YY65 pKa = 11.32FLPYY69 pKa = 10.36LNDD72 pKa = 3.31VAIDD76 pKa = 3.69NVGEE80 pKa = 4.07IVEE83 pKa = 4.56EE84 pKa = 3.99IMDD87 pKa = 3.83EE88 pKa = 4.46FEE90 pKa = 5.37IEE92 pKa = 4.12AQFICYY98 pKa = 9.95DD99 pKa = 3.98LNKK102 pKa = 10.38EE103 pKa = 4.09KK104 pKa = 10.83YY105 pKa = 9.87DD106 pKa = 3.64YY107 pKa = 11.52LEE109 pKa = 4.14FVAIFFEE116 pKa = 4.72EE117 pKa = 5.24GSHH120 pKa = 7.47DD121 pKa = 3.74IEE123 pKa = 6.05DD124 pKa = 4.02VLDD127 pKa = 3.8SLNII131 pKa = 3.71

Molecular weight: 15.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I0Z7E3|A0A1I0Z7E3_9CLOT Transcriptional regulator PadR family OS=Clostridium frigidicarnis OX=84698 GN=SAMN04488528_101821 PE=4 SV=1
MM1 pKa = 7.34ARR3 pKa = 11.84KK4 pKa = 9.46ALIEE8 pKa = 4.12KK9 pKa = 8.84WNKK12 pKa = 6.26TPKK15 pKa = 8.84YY16 pKa = 10.18ASRR19 pKa = 11.84AYY21 pKa = 8.89TRR23 pKa = 11.84CRR25 pKa = 11.84LCGRR29 pKa = 11.84PHH31 pKa = 6.07AVLRR35 pKa = 11.84KK36 pKa = 9.54FGVCRR41 pKa = 11.84ICFRR45 pKa = 11.84EE46 pKa = 3.81LAYY49 pKa = 10.04KK50 pKa = 10.84GEE52 pKa = 4.18IPGCRR57 pKa = 11.84KK58 pKa = 10.18ASWW61 pKa = 3.01

Molecular weight: 7.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3915

0

3915

1178405

24

3844

301.0

34.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.096 ± 0.046

1.296 ± 0.017

5.636 ± 0.032

7.444 ± 0.05

4.494 ± 0.033

6.341 ± 0.041

1.344 ± 0.013

10.064 ± 0.046

9.259 ± 0.042

9.158 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.618 ± 0.018

6.759 ± 0.04

2.686 ± 0.023

2.195 ± 0.017

3.206 ± 0.024

6.376 ± 0.033

4.845 ± 0.032

6.41 ± 0.035

0.67 ± 0.014

4.104 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski