Psychromonas sp. psych-6C06
Average proteome isoelectric point is 6.21
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3200 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2N0X3Y5|A0A2N0X3Y5_9GAMM Lytic murein transglycosylase OS=Psychromonas sp. psych-6C06 OX=2058089 GN=CW745_01995 PE=4 SV=1
MM1 pKa = 8.2 FMMKK5 pKa = 9.9 RR6 pKa = 11.84 KK7 pKa = 9.66 ALLCLPVCLSTLVSMHH23 pKa = 6.43 SLANNQDD30 pKa = 2.99 ARR32 pKa = 11.84 SFAMGGVGVSTSNFLTSSLHH52 pKa = 5.85 NPALAAKK59 pKa = 9.52 FDD61 pKa = 3.66 NSDD64 pKa = 4.1 DD65 pKa = 3.56 IGFLFPSFAANVNNIADD82 pKa = 3.84 TVDD85 pKa = 3.59 NIEE88 pKa = 4.45 NFSDD92 pKa = 3.86 VYY94 pKa = 11.7 DD95 pKa = 3.88 EE96 pKa = 4.95 FKK98 pKa = 11.15 SIATPTLDD106 pKa = 4.04 DD107 pKa = 3.81 AQKK110 pKa = 11.04 VVDD113 pKa = 4.77 EE114 pKa = 5.05 LYY116 pKa = 11.05 SLQGDD121 pKa = 3.78 PASINAGSQIALAIPNDD138 pKa = 3.32 IVAVNLYY145 pKa = 9.62 AQAYY149 pKa = 7.47 VDD151 pKa = 4.04 AMVFADD157 pKa = 5.0 IADD160 pKa = 3.99 TDD162 pKa = 4.3 LEE164 pKa = 4.95 ADD166 pKa = 3.9 NILNQDD172 pKa = 3.98 LNSNALTMGVLVSEE186 pKa = 5.18 FGVTLAKK193 pKa = 10.39 YY194 pKa = 9.54 HH195 pKa = 5.94 QFDD198 pKa = 3.92 NSTLYY203 pKa = 11.02 YY204 pKa = 10.71 GLTPKK209 pKa = 10.4 YY210 pKa = 9.28 QTVDD214 pKa = 3.46 TINFVSNIDD223 pKa = 3.91 NVDD226 pKa = 3.91 FDD228 pKa = 5.05 DD229 pKa = 4.53 WDD231 pKa = 3.64 NDD233 pKa = 3.63 SYY235 pKa = 11.83 QNSEE239 pKa = 3.62 GNMNVDD245 pKa = 4.25 LGIAYY250 pKa = 7.87 QHH252 pKa = 5.94 QNGFGLGLAAKK263 pKa = 9.79 NLLKK267 pKa = 10.4 QSYY270 pKa = 6.46 EE271 pKa = 4.05 TEE273 pKa = 4.45 SISGVQGHH281 pKa = 5.66 YY282 pKa = 10.39 EE283 pKa = 3.71 ITPVYY288 pKa = 9.15 TLGGHH293 pKa = 5.38 YY294 pKa = 10.11 EE295 pKa = 4.05 SSFVIAALDD304 pKa = 3.38 IQLNEE309 pKa = 3.97 SEE311 pKa = 5.15 GYY313 pKa = 10.52 SKK315 pKa = 9.95 VTGINTNFNSDD326 pKa = 2.87 NDD328 pKa = 3.68 NRR330 pKa = 11.84 QFASLGVEE338 pKa = 4.2 FVPMNWIKK346 pKa = 10.75 LRR348 pKa = 11.84 AGYY351 pKa = 10.38 RR352 pKa = 11.84 SDD354 pKa = 4.58 LSDD357 pKa = 5.15 NIDD360 pKa = 3.6 DD361 pKa = 5.41 SITAGIGFSVVNIFHH376 pKa = 7.23 IDD378 pKa = 3.43 LSGSYY383 pKa = 10.63 SDD385 pKa = 4.26 NNNMGAAAQTSFTFF399 pKa = 3.85
Molecular weight: 43.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.727
IPC2_protein 3.897
IPC_protein 3.935
Toseland 3.694
ProMoST 4.101
Dawson 3.935
Bjellqvist 4.088
Wikipedia 3.884
Rodwell 3.757
Grimsley 3.605
Solomon 3.923
Lehninger 3.884
Nozaki 4.037
DTASelect 4.329
Thurlkill 3.757
EMBOSS 3.897
Sillero 4.05
Patrickios 1.074
IPC_peptide 3.923
IPC2_peptide 4.024
IPC2.peptide.svr19 3.919
Protein with the highest isoelectric point:
>tr|A0A2N0WXC0|A0A2N0WXC0_9GAMM Uncharacterized protein OS=Psychromonas sp. psych-6C06 OX=2058089 GN=CW745_13085 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLKK11 pKa = 10.14 RR12 pKa = 11.84 KK13 pKa = 8.99 RR14 pKa = 11.84 SHH16 pKa = 6.31 GFRR19 pKa = 11.84 TRR21 pKa = 11.84 MATKK25 pKa = 10.2 NGRR28 pKa = 11.84 NVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.5 GRR39 pKa = 11.84 ASLSAA44 pKa = 3.83
Molecular weight: 5.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.199
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.647
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.466
Sillero 12.954
Patrickios 12.369
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.154
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3200
0
3200
1074385
34
4285
335.7
37.39
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.215 ± 0.046
1.046 ± 0.014
5.503 ± 0.052
6.121 ± 0.041
4.346 ± 0.031
6.356 ± 0.047
2.165 ± 0.026
7.111 ± 0.037
5.899 ± 0.047
10.709 ± 0.058
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.404 ± 0.027
4.843 ± 0.039
3.598 ± 0.028
4.801 ± 0.041
3.888 ± 0.032
6.841 ± 0.041
5.414 ± 0.045
6.521 ± 0.035
1.107 ± 0.016
3.109 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here