Sulfolobus sp. SCGC AB-777_G05
Average proteome isoelectric point is 6.83
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 297 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2T9WK47|A0A2T9WK47_9CREN Peptidase U32 OS=Sulfolobus sp. SCGC AB-777_G05 OX=1987490 GN=DDW01_00310 PE=4 SV=1
MM1 pKa = 7.31 FKK3 pKa = 10.62 KK4 pKa = 10.51 FYY6 pKa = 10.79 EE7 pKa = 4.32 EE8 pKa = 4.25 GNEE11 pKa = 4.08 VEE13 pKa = 5.36 YY14 pKa = 9.26 EE15 pKa = 4.02 TEE17 pKa = 4.07 NPVKK21 pKa = 10.54 VAEE24 pKa = 4.32 IPDD27 pKa = 5.08 LISQVINTDD36 pKa = 3.1 AEE38 pKa = 4.32 VIDD41 pKa = 4.15 IYY43 pKa = 11.06 FEE45 pKa = 4.02 EE46 pKa = 5.0 VEE48 pKa = 4.35 VEE50 pKa = 4.41 GKK52 pKa = 10.19 DD53 pKa = 3.08 IYY55 pKa = 9.81 PTVVVDD61 pKa = 4.88 EE62 pKa = 4.65 YY63 pKa = 11.57 LPNSNPAYY71 pKa = 10.44 LIYY74 pKa = 10.75 SKK76 pKa = 10.76 EE77 pKa = 3.82 LDD79 pKa = 4.15 DD80 pKa = 5.58 IKK82 pKa = 10.66 WEE84 pKa = 4.07 EE85 pKa = 4.03 MKK87 pKa = 11.04 SKK89 pKa = 10.17 IKK91 pKa = 10.6 EE92 pKa = 3.84 IVTGKK97 pKa = 10.52 GYY99 pKa = 10.2 NVCDD103 pKa = 3.4 EE104 pKa = 4.34 DD105 pKa = 3.73 VFYY108 pKa = 10.82 ISFCKK113 pKa = 10.37
Molecular weight: 13.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.04
IPC2_protein 4.151
IPC_protein 4.062
Toseland 3.897
ProMoST 4.177
Dawson 4.012
Bjellqvist 4.164
Wikipedia 3.872
Rodwell 3.897
Grimsley 3.808
Solomon 3.999
Lehninger 3.948
Nozaki 4.126
DTASelect 4.24
Thurlkill 3.91
EMBOSS 3.897
Sillero 4.164
Patrickios 2.778
IPC_peptide 4.012
IPC2_peptide 4.164
IPC2.peptide.svr19 4.078
Protein with the highest isoelectric point:
>tr|A0A2T9WK95|A0A2T9WK95_9CREN Serine/threonine protein kinase OS=Sulfolobus sp. SCGC AB-777_G05 OX=1987490 GN=DDW01_00075 PE=4 SV=1
MM1 pKa = 7.78 ILGLPFWRR9 pKa = 11.84 RR10 pKa = 11.84 EE11 pKa = 3.81 RR12 pKa = 11.84 LKK14 pKa = 10.92 GYY16 pKa = 8.35 LTIFTIMTNVVRR28 pKa = 11.84 EE29 pKa = 4.25 LVSEE33 pKa = 4.21 LNLRR37 pKa = 11.84 SVEE40 pKa = 3.95 AFLKK44 pKa = 10.5 FVEE47 pKa = 5.25 RR48 pKa = 11.84 YY49 pKa = 9.94 LGGPPGLMKK58 pKa = 10.24 IFKK61 pKa = 10.26 LRR63 pKa = 3.39
Molecular weight: 7.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.316
IPC2_protein 9.78
IPC_protein 10.613
Toseland 10.789
ProMoST 10.95
Dawson 10.877
Bjellqvist 10.584
Wikipedia 11.082
Rodwell 11.111
Grimsley 10.921
Solomon 11.008
Lehninger 10.965
Nozaki 10.745
DTASelect 10.584
Thurlkill 10.774
EMBOSS 11.184
Sillero 10.804
Patrickios 10.906
IPC_peptide 11.008
IPC2_peptide 9.355
IPC2.peptide.svr19 8.721
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
297
0
297
68966
34
1070
232.2
26.43
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.14 ± 0.13
0.782 ± 0.057
4.785 ± 0.123
7.795 ± 0.192
4.408 ± 0.113
6.206 ± 0.148
1.321 ± 0.052
9.448 ± 0.154
8.426 ± 0.196
10.521 ± 0.157
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.026 ± 0.063
4.695 ± 0.109
3.809 ± 0.102
1.952 ± 0.062
4.402 ± 0.111
6.447 ± 0.134
4.405 ± 0.084
7.588 ± 0.121
1.063 ± 0.068
4.782 ± 0.095
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here