Pseudomonas phage shl2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Ghunavirus; Pseudomonas virus shl2

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A170PBD5|A0A170PBD5_9CAUD Uncharacterized protein OS=Pseudomonas phage shl2 OX=1729933 GN=SHL_00047 PE=4 SV=1
MM1 pKa = 7.31LAIIEE6 pKa = 4.7HH7 pKa = 6.63YY8 pKa = 9.95IHH10 pKa = 6.77NPDD13 pKa = 5.85DD14 pKa = 4.24IPDD17 pKa = 4.16IPPQSAEE24 pKa = 3.95YY25 pKa = 11.16LNVRR29 pKa = 11.84LNASYY34 pKa = 10.84LITTGAVDD42 pKa = 4.05EE43 pKa = 4.57LRR45 pKa = 11.84KK46 pKa = 10.06AGYY49 pKa = 9.25SEE51 pKa = 4.43QYY53 pKa = 9.38IAGFIDD59 pKa = 4.3GCHH62 pKa = 5.99SATEE66 pKa = 4.31IVDD69 pKa = 4.56FMQEE73 pKa = 3.93SQKK76 pKa = 11.12NKK78 pKa = 9.96EE79 pKa = 4.16DD80 pKa = 3.35

Molecular weight: 9.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A160SWZ3|A0A160SWZ3_9CAUD Uncharacterized protein OS=Pseudomonas phage shl2 OX=1729933 GN=SHL_00023 PE=4 SV=1
MM1 pKa = 7.63ILLHH5 pKa = 5.95SNKK8 pKa = 8.73GTGHH12 pKa = 4.69YY13 pKa = 7.22TVRR16 pKa = 11.84SQRR19 pKa = 11.84EE20 pKa = 3.54IVAWLGKK27 pKa = 10.43KK28 pKa = 9.33PMHH31 pKa = 7.43AILQDD36 pKa = 3.92PNKK39 pKa = 9.85ATYY42 pKa = 10.31LVTKK46 pKa = 9.09GTFADD51 pKa = 3.64SRR53 pKa = 11.84KK54 pKa = 9.86LGRR57 pKa = 11.84VVLKK61 pKa = 10.38QYY63 pKa = 9.03TGNWPRR69 pKa = 11.84CALVWKK75 pKa = 10.04IVKK78 pKa = 9.87EE79 pKa = 4.27VVNGG83 pKa = 3.71

Molecular weight: 9.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

50

0

50

12582

45

1399

251.6

27.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.237 ± 0.538

0.89 ± 0.143

6.581 ± 0.265

6.406 ± 0.369

3.704 ± 0.202

7.948 ± 0.294

1.987 ± 0.225

5.007 ± 0.235

6.009 ± 0.376

8.051 ± 0.293

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.623 ± 0.14

4.26 ± 0.345

3.926 ± 0.211

4.308 ± 0.352

5.619 ± 0.2

5.834 ± 0.382

5.603 ± 0.297

6.485 ± 0.328

1.526 ± 0.14

2.996 ± 0.173
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski