Amino acid dipepetide frequency for Pseudomonas phage shl2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.207AlaAla: 11.207 ± 1.158
0.795AlaCys: 0.795 ± 0.258
6.518AlaAsp: 6.518 ± 0.733
5.008AlaGlu: 5.008 ± 0.576
2.941AlaPhe: 2.941 ± 0.5
8.425AlaGly: 8.425 ± 0.736
1.908AlaHis: 1.908 ± 0.541
5.643AlaIle: 5.643 ± 0.834
6.359AlaLys: 6.359 ± 0.533
7.313AlaLeu: 7.313 ± 0.845
3.02AlaMet: 3.02 ± 0.605
5.484AlaAsn: 5.484 ± 0.74
3.577AlaPro: 3.577 ± 0.549
5.008AlaGln: 5.008 ± 0.567
6.12AlaArg: 6.12 ± 0.695
6.756AlaSer: 6.756 ± 0.969
6.438AlaThr: 6.438 ± 1.002
5.802AlaVal: 5.802 ± 0.658
1.272AlaTrp: 1.272 ± 0.348
2.782AlaTyr: 2.782 ± 0.563
0.0AlaXaa: 0.0 ± 0.0
Cys
1.351CysAla: 1.351 ± 0.307
0.0CysCys: 0.0 ± 0.0
0.477CysAsp: 0.477 ± 0.234
0.477CysGlu: 0.477 ± 0.246
0.636CysPhe: 0.636 ± 0.238
0.397CysGly: 0.397 ± 0.17
0.715CysHis: 0.715 ± 0.295
0.318CysIle: 0.318 ± 0.17
0.636CysLys: 0.636 ± 0.245
0.795CysLeu: 0.795 ± 0.253
0.159CysMet: 0.159 ± 0.097
0.079CysAsn: 0.079 ± 0.077
0.318CysPro: 0.318 ± 0.15
0.318CysGln: 0.318 ± 0.143
0.715CysArg: 0.715 ± 0.244
0.477CysSer: 0.477 ± 0.216
0.318CysThr: 0.318 ± 0.163
0.556CysVal: 0.556 ± 0.187
0.0CysTrp: 0.0 ± 0.0
0.159CysTyr: 0.159 ± 0.123
0.0CysXaa: 0.0 ± 0.0
Asp
6.438AspAla: 6.438 ± 0.755
0.477AspCys: 0.477 ± 0.202
4.849AspAsp: 4.849 ± 0.641
4.769AspGlu: 4.769 ± 0.707
3.259AspPhe: 3.259 ± 0.431
6.756AspGly: 6.756 ± 0.648
1.431AspHis: 1.431 ± 0.351
3.418AspIle: 3.418 ± 0.627
3.815AspLys: 3.815 ± 0.46
4.213AspLeu: 4.213 ± 0.605
2.146AspMet: 2.146 ± 0.353
1.908AspAsn: 1.908 ± 0.393
2.782AspPro: 2.782 ± 0.575
2.544AspGln: 2.544 ± 0.386
2.861AspArg: 2.861 ± 0.504
3.179AspSer: 3.179 ± 0.52
2.861AspThr: 2.861 ± 0.434
4.69AspVal: 4.69 ± 0.656
1.272AspTrp: 1.272 ± 0.391
2.067AspTyr: 2.067 ± 0.394
0.0AspXaa: 0.0 ± 0.0
Glu
7.233GluAla: 7.233 ± 0.65
0.874GluCys: 0.874 ± 0.312
4.69GluAsp: 4.69 ± 0.435
2.941GluGlu: 2.941 ± 0.646
2.702GluPhe: 2.702 ± 0.373
4.133GluGly: 4.133 ± 0.562
1.351GluHis: 1.351 ± 0.276
3.179GluIle: 3.179 ± 0.503
3.02GluLys: 3.02 ± 0.472
4.531GluLeu: 4.531 ± 0.722
1.908GluMet: 1.908 ± 0.29
2.146GluAsn: 2.146 ± 0.401
1.987GluPro: 1.987 ± 0.449
3.577GluGln: 3.577 ± 0.524
4.451GluArg: 4.451 ± 0.7
3.1GluSer: 3.1 ± 0.433
3.895GluThr: 3.895 ± 0.582
4.69GluVal: 4.69 ± 0.689
1.113GluTrp: 1.113 ± 0.469
2.544GluTyr: 2.544 ± 0.411
0.0GluXaa: 0.0 ± 0.0
Phe
3.736PheAla: 3.736 ± 0.672
0.636PheCys: 0.636 ± 0.211
2.385PheAsp: 2.385 ± 0.403
1.987PheGlu: 1.987 ± 0.435
1.431PhePhe: 1.431 ± 0.372
2.702PheGly: 2.702 ± 0.544
1.192PheHis: 1.192 ± 0.304
1.351PheIle: 1.351 ± 0.404
2.464PheLys: 2.464 ± 0.579
2.623PheLeu: 2.623 ± 0.41
1.272PheMet: 1.272 ± 0.302
1.908PheAsn: 1.908 ± 0.409
1.669PhePro: 1.669 ± 0.378
0.954PheGln: 0.954 ± 0.274
1.987PheArg: 1.987 ± 0.309
1.908PheSer: 1.908 ± 0.405
2.941PheThr: 2.941 ± 0.552
2.702PheVal: 2.702 ± 0.342
0.477PheTrp: 0.477 ± 0.23
0.715PheTyr: 0.715 ± 0.21
0.0PheXaa: 0.0 ± 0.0
Gly
7.154GlyAla: 7.154 ± 0.889
0.477GlyCys: 0.477 ± 0.155
4.292GlyAsp: 4.292 ± 0.519
5.564GlyGlu: 5.564 ± 0.61
3.1GlyPhe: 3.1 ± 0.386
6.756GlyGly: 6.756 ± 0.715
1.51GlyHis: 1.51 ± 0.437
4.928GlyIle: 4.928 ± 0.705
4.054GlyLys: 4.054 ± 0.778
6.359GlyLeu: 6.359 ± 0.883
1.749GlyMet: 1.749 ± 0.378
3.179GlyAsn: 3.179 ± 0.679
2.623GlyPro: 2.623 ± 0.4
3.02GlyGln: 3.02 ± 0.478
4.61GlyArg: 4.61 ± 0.624
5.961GlySer: 5.961 ± 0.71
4.849GlyThr: 4.849 ± 0.492
4.531GlyVal: 4.531 ± 0.602
1.431GlyTrp: 1.431 ± 0.313
3.338GlyTyr: 3.338 ± 0.513
0.0GlyXaa: 0.0 ± 0.0
His
1.351HisAla: 1.351 ± 0.375
0.318HisCys: 0.318 ± 0.204
1.272HisAsp: 1.272 ± 0.335
1.192HisGlu: 1.192 ± 0.351
0.795HisPhe: 0.795 ± 0.248
2.464HisGly: 2.464 ± 0.472
0.159HisHis: 0.159 ± 0.132
1.351HisIle: 1.351 ± 0.314
0.795HisLys: 0.795 ± 0.257
2.226HisLeu: 2.226 ± 0.484
0.874HisMet: 0.874 ± 0.236
0.636HisAsn: 0.636 ± 0.179
0.874HisPro: 0.874 ± 0.397
0.477HisGln: 0.477 ± 0.159
0.715HisArg: 0.715 ± 0.249
1.192HisSer: 1.192 ± 0.376
0.636HisThr: 0.636 ± 0.199
1.272HisVal: 1.272 ± 0.366
0.556HisTrp: 0.556 ± 0.235
0.715HisTyr: 0.715 ± 0.195
0.0HisXaa: 0.0 ± 0.0
Ile
5.564IleAla: 5.564 ± 0.612
0.715IleCys: 0.715 ± 0.221
2.941IleAsp: 2.941 ± 0.447
3.974IleGlu: 3.974 ± 0.665
1.033IlePhe: 1.033 ± 0.237
3.02IleGly: 3.02 ± 0.477
1.033IleHis: 1.033 ± 0.341
2.226IleIle: 2.226 ± 0.386
3.02IleLys: 3.02 ± 0.372
4.928IleLeu: 4.928 ± 0.547
0.715IleMet: 0.715 ± 0.25
2.305IleAsn: 2.305 ± 0.583
2.146IlePro: 2.146 ± 0.491
2.067IleGln: 2.067 ± 0.37
3.338IleArg: 3.338 ± 0.503
2.782IleSer: 2.782 ± 0.368
3.179IleThr: 3.179 ± 0.59
3.338IleVal: 3.338 ± 0.572
0.477IleTrp: 0.477 ± 0.154
1.272IleTyr: 1.272 ± 0.338
0.0IleXaa: 0.0 ± 0.0
Lys
7.551LysAla: 7.551 ± 0.89
0.397LysCys: 0.397 ± 0.168
4.451LysAsp: 4.451 ± 0.673
4.849LysGlu: 4.849 ± 0.642
1.987LysPhe: 1.987 ± 0.403
4.531LysGly: 4.531 ± 0.658
1.192LysHis: 1.192 ± 0.481
2.544LysIle: 2.544 ± 0.553
3.418LysLys: 3.418 ± 0.649
5.484LysLeu: 5.484 ± 0.529
1.272LysMet: 1.272 ± 0.321
1.749LysAsn: 1.749 ± 0.346
2.544LysPro: 2.544 ± 0.435
2.702LysGln: 2.702 ± 0.606
3.338LysArg: 3.338 ± 0.662
2.146LysSer: 2.146 ± 0.4
2.702LysThr: 2.702 ± 0.45
4.849LysVal: 4.849 ± 0.644
0.874LysTrp: 0.874 ± 0.259
1.51LysTyr: 1.51 ± 0.335
0.0LysXaa: 0.0 ± 0.0
Leu
8.187LeuAla: 8.187 ± 0.97
0.397LeuCys: 0.397 ± 0.201
5.643LeuAsp: 5.643 ± 0.611
5.246LeuGlu: 5.246 ± 0.834
2.464LeuPhe: 2.464 ± 0.433
5.325LeuGly: 5.325 ± 0.518
1.59LeuHis: 1.59 ± 0.349
5.167LeuIle: 5.167 ± 0.686
6.12LeuLys: 6.12 ± 0.695
4.849LeuLeu: 4.849 ± 0.578
1.51LeuMet: 1.51 ± 0.283
4.292LeuAsn: 4.292 ± 0.705
2.305LeuPro: 2.305 ± 0.346
4.69LeuGln: 4.69 ± 0.653
4.928LeuArg: 4.928 ± 0.709
4.849LeuSer: 4.849 ± 0.817
4.451LeuThr: 4.451 ± 0.672
5.246LeuVal: 5.246 ± 0.668
1.113LeuTrp: 1.113 ± 0.3
2.146LeuTyr: 2.146 ± 0.341
0.0LeuXaa: 0.0 ± 0.0
Met
2.782MetAla: 2.782 ± 0.368
0.318MetCys: 0.318 ± 0.171
2.385MetAsp: 2.385 ± 0.319
1.669MetGlu: 1.669 ± 0.378
0.795MetPhe: 0.795 ± 0.257
1.987MetGly: 1.987 ± 0.326
0.238MetHis: 0.238 ± 0.125
1.749MetIle: 1.749 ± 0.306
1.192MetLys: 1.192 ± 0.306
2.782MetLeu: 2.782 ± 0.477
0.636MetMet: 0.636 ± 0.207
1.351MetAsn: 1.351 ± 0.369
1.51MetPro: 1.51 ± 0.327
1.192MetGln: 1.192 ± 0.323
0.636MetArg: 0.636 ± 0.23
1.908MetSer: 1.908 ± 0.339
1.272MetThr: 1.272 ± 0.266
1.272MetVal: 1.272 ± 0.266
0.238MetTrp: 0.238 ± 0.118
0.318MetTyr: 0.318 ± 0.182
0.0MetXaa: 0.0 ± 0.0
Asn
4.213AsnAla: 4.213 ± 0.556
0.477AsnCys: 0.477 ± 0.191
2.385AsnAsp: 2.385 ± 0.381
2.305AsnGlu: 2.305 ± 0.548
1.669AsnPhe: 1.669 ± 0.38
4.213AsnGly: 4.213 ± 0.673
0.556AsnHis: 0.556 ± 0.248
1.987AsnIle: 1.987 ± 0.425
1.351AsnLys: 1.351 ± 0.418
3.577AsnLeu: 3.577 ± 0.6
1.033AsnMet: 1.033 ± 0.281
1.908AsnAsn: 1.908 ± 0.312
2.861AsnPro: 2.861 ± 0.358
2.067AsnGln: 2.067 ± 0.407
2.067AsnArg: 2.067 ± 0.405
3.179AsnSer: 3.179 ± 0.795
2.623AsnThr: 2.623 ± 0.74
2.067AsnVal: 2.067 ± 0.451
0.795AsnTrp: 0.795 ± 0.251
1.272AsnTyr: 1.272 ± 0.317
0.0AsnXaa: 0.0 ± 0.0
Pro
3.179ProAla: 3.179 ± 0.577
0.556ProCys: 0.556 ± 0.183
3.974ProAsp: 3.974 ± 0.629
3.815ProGlu: 3.815 ± 0.541
1.51ProPhe: 1.51 ± 0.26
2.305ProGly: 2.305 ± 0.341
0.954ProHis: 0.954 ± 0.263
1.59ProIle: 1.59 ± 0.297
2.544ProLys: 2.544 ± 0.433
2.861ProLeu: 2.861 ± 0.487
0.795ProMet: 0.795 ± 0.215
2.305ProAsn: 2.305 ± 0.33
0.954ProPro: 0.954 ± 0.247
1.51ProGln: 1.51 ± 0.324
1.669ProArg: 1.669 ± 0.392
2.067ProSer: 2.067 ± 0.312
2.067ProThr: 2.067 ± 0.442
2.941ProVal: 2.941 ± 0.38
0.477ProTrp: 0.477 ± 0.189
1.192ProTyr: 1.192 ± 0.42
0.0ProXaa: 0.0 ± 0.0
Gln
5.484GlnAla: 5.484 ± 0.941
0.159GlnCys: 0.159 ± 0.117
2.385GlnAsp: 2.385 ± 0.405
3.179GlnGlu: 3.179 ± 0.574
2.226GlnPhe: 2.226 ± 0.302
3.895GlnGly: 3.895 ± 0.512
0.715GlnHis: 0.715 ± 0.187
1.59GlnIle: 1.59 ± 0.374
2.544GlnLys: 2.544 ± 0.446
4.292GlnLeu: 4.292 ± 0.567
1.192GlnMet: 1.192 ± 0.281
1.351GlnAsn: 1.351 ± 0.305
0.874GlnPro: 0.874 ± 0.254
2.067GlnGln: 2.067 ± 0.721
2.782GlnArg: 2.782 ± 0.723
2.385GlnSer: 2.385 ± 0.504
1.51GlnThr: 1.51 ± 0.365
2.385GlnVal: 2.385 ± 0.382
0.715GlnTrp: 0.715 ± 0.229
1.351GlnTyr: 1.351 ± 0.374
0.0GlnXaa: 0.0 ± 0.0
Arg
4.69ArgAla: 4.69 ± 0.485
0.477ArgCys: 0.477 ± 0.258
3.338ArgAsp: 3.338 ± 0.412
3.815ArgGlu: 3.815 ± 0.488
1.987ArgPhe: 1.987 ± 0.47
4.451ArgGly: 4.451 ± 0.5
1.113ArgHis: 1.113 ± 0.343
2.782ArgIle: 2.782 ± 0.445
4.054ArgLys: 4.054 ± 0.601
5.405ArgLeu: 5.405 ± 0.457
1.351ArgMet: 1.351 ± 0.26
2.305ArgAsn: 2.305 ± 0.388
1.669ArgPro: 1.669 ± 0.409
2.861ArgGln: 2.861 ± 0.401
3.02ArgArg: 3.02 ± 0.441
4.133ArgSer: 4.133 ± 0.561
2.941ArgThr: 2.941 ± 0.567
2.941ArgVal: 2.941 ± 0.376
0.795ArgTrp: 0.795 ± 0.311
2.067ArgTyr: 2.067 ± 0.304
0.0ArgXaa: 0.0 ± 0.0
Ser
6.836SerAla: 6.836 ± 0.901
0.397SerCys: 0.397 ± 0.159
4.531SerAsp: 4.531 ± 0.527
2.861SerGlu: 2.861 ± 0.402
2.861SerPhe: 2.861 ± 0.508
4.531SerGly: 4.531 ± 0.777
1.272SerHis: 1.272 ± 0.281
2.782SerIle: 2.782 ± 0.504
3.418SerLys: 3.418 ± 0.514
3.497SerLeu: 3.497 ± 0.566
1.908SerMet: 1.908 ± 0.429
2.067SerAsn: 2.067 ± 0.45
2.702SerPro: 2.702 ± 0.398
2.385SerGln: 2.385 ± 0.48
3.179SerArg: 3.179 ± 0.533
3.974SerSer: 3.974 ± 0.778
2.385SerThr: 2.385 ± 0.404
3.418SerVal: 3.418 ± 0.507
0.954SerTrp: 0.954 ± 0.245
2.385SerTyr: 2.385 ± 0.517
0.0SerXaa: 0.0 ± 0.0
Thr
4.531ThrAla: 4.531 ± 0.799
0.397ThrCys: 0.397 ± 0.191
3.179ThrAsp: 3.179 ± 0.498
3.338ThrGlu: 3.338 ± 0.527
2.067ThrPhe: 2.067 ± 0.481
5.167ThrGly: 5.167 ± 0.605
0.556ThrHis: 0.556 ± 0.225
3.338ThrIle: 3.338 ± 0.447
4.372ThrLys: 4.372 ± 0.503
5.723ThrLeu: 5.723 ± 0.743
1.113ThrMet: 1.113 ± 0.284
2.544ThrAsn: 2.544 ± 0.318
3.259ThrPro: 3.259 ± 0.339
1.51ThrGln: 1.51 ± 0.246
2.146ThrArg: 2.146 ± 0.332
2.544ThrSer: 2.544 ± 0.426
3.418ThrThr: 3.418 ± 0.827
4.69ThrVal: 4.69 ± 0.566
0.715ThrTrp: 0.715 ± 0.193
1.431ThrTyr: 1.431 ± 0.339
0.0ThrXaa: 0.0 ± 0.0
Val
6.279ValAla: 6.279 ± 0.787
0.636ValCys: 0.636 ± 0.237
3.736ValAsp: 3.736 ± 0.447
4.133ValGlu: 4.133 ± 0.606
1.987ValPhe: 1.987 ± 0.396
4.69ValGly: 4.69 ± 0.604
1.272ValHis: 1.272 ± 0.391
2.623ValIle: 2.623 ± 0.594
3.895ValLys: 3.895 ± 0.563
4.69ValLeu: 4.69 ± 0.769
2.146ValMet: 2.146 ± 0.442
2.861ValAsn: 2.861 ± 0.559
3.338ValPro: 3.338 ± 0.694
2.544ValGln: 2.544 ± 0.452
4.928ValArg: 4.928 ± 0.696
3.338ValSer: 3.338 ± 0.463
4.372ValThr: 4.372 ± 0.571
4.292ValVal: 4.292 ± 0.769
1.272ValTrp: 1.272 ± 0.296
1.828ValTyr: 1.828 ± 0.383
0.0ValXaa: 0.0 ± 0.0
Trp
1.351TrpAla: 1.351 ± 0.334
0.079TrpCys: 0.079 ± 0.08
0.556TrpAsp: 0.556 ± 0.2
0.715TrpGlu: 0.715 ± 0.204
0.636TrpPhe: 0.636 ± 0.23
0.874TrpGly: 0.874 ± 0.228
0.636TrpHis: 0.636 ± 0.281
0.477TrpIle: 0.477 ± 0.181
0.954TrpLys: 0.954 ± 0.317
1.908TrpLeu: 1.908 ± 0.401
0.556TrpMet: 0.556 ± 0.175
0.954TrpAsn: 0.954 ± 0.213
0.318TrpPro: 0.318 ± 0.13
0.397TrpGln: 0.397 ± 0.197
0.874TrpArg: 0.874 ± 0.195
1.033TrpSer: 1.033 ± 0.278
0.954TrpThr: 0.954 ± 0.273
1.51TrpVal: 1.51 ± 0.428
0.238TrpTrp: 0.238 ± 0.13
0.238TrpTyr: 0.238 ± 0.12
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.259TyrAla: 3.259 ± 0.468
0.318TyrCys: 0.318 ± 0.165
1.828TyrAsp: 1.828 ± 0.394
2.226TyrGlu: 2.226 ± 0.458
0.954TyrPhe: 0.954 ± 0.266
2.861TyrGly: 2.861 ± 0.349
0.477TyrHis: 0.477 ± 0.229
1.033TyrIle: 1.033 ± 0.296
2.067TyrLys: 2.067 ± 0.382
2.464TyrLeu: 2.464 ± 0.445
0.795TyrMet: 0.795 ± 0.198
1.272TyrAsn: 1.272 ± 0.3
0.954TyrPro: 0.954 ± 0.202
1.192TyrGln: 1.192 ± 0.262
1.828TyrArg: 1.828 ± 0.309
1.431TyrSer: 1.431 ± 0.273
2.226TyrThr: 2.226 ± 0.48
1.669TyrVal: 1.669 ± 0.327
0.477TyrTrp: 0.477 ± 0.193
0.636TyrTyr: 0.636 ± 0.25
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 50 proteins (12582 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski