Amino acid dipepetide frequency for Psychromonas sp. RZ22

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.493AlaAla: 6.493 ± 0.115
0.974AlaCys: 0.974 ± 0.033
4.289AlaAsp: 4.289 ± 0.079
5.031AlaGlu: 5.031 ± 0.093
3.384AlaPhe: 3.384 ± 0.067
5.473AlaGly: 5.473 ± 0.114
1.55AlaHis: 1.55 ± 0.044
6.317AlaIle: 6.317 ± 0.086
5.126AlaLys: 5.126 ± 0.08
9.118AlaLeu: 9.118 ± 0.127
2.274AlaMet: 2.274 ± 0.053
3.514AlaAsn: 3.514 ± 0.062
2.447AlaPro: 2.447 ± 0.058
3.504AlaGln: 3.504 ± 0.071
2.916AlaArg: 2.916 ± 0.066
4.961AlaSer: 4.961 ± 0.083
4.535AlaThr: 4.535 ± 0.083
5.48AlaVal: 5.48 ± 0.091
0.874AlaTrp: 0.874 ± 0.035
2.386AlaTyr: 2.386 ± 0.051
0.0AlaXaa: 0.0 ± 0.0
Cys
0.775CysAla: 0.775 ± 0.028
0.178CysCys: 0.178 ± 0.014
0.58CysAsp: 0.58 ± 0.028
0.648CysGlu: 0.648 ± 0.028
0.49CysPhe: 0.49 ± 0.024
0.834CysGly: 0.834 ± 0.032
0.336CysHis: 0.336 ± 0.018
0.729CysIle: 0.729 ± 0.027
0.543CysLys: 0.543 ± 0.025
1.054CysLeu: 1.054 ± 0.037
0.22CysMet: 0.22 ± 0.017
0.396CysAsn: 0.396 ± 0.023
0.428CysPro: 0.428 ± 0.025
0.45CysGln: 0.45 ± 0.021
0.392CysArg: 0.392 ± 0.023
0.756CysSer: 0.756 ± 0.032
0.506CysThr: 0.506 ± 0.021
0.653CysVal: 0.653 ± 0.031
0.121CysTrp: 0.121 ± 0.012
0.379CysTyr: 0.379 ± 0.019
0.0CysXaa: 0.0 ± 0.0
Asp
4.259AspAla: 4.259 ± 0.089
0.564AspCys: 0.564 ± 0.025
2.851AspAsp: 2.851 ± 0.073
3.47AspGlu: 3.47 ± 0.078
2.526AspPhe: 2.526 ± 0.053
3.422AspGly: 3.422 ± 0.115
1.129AspHis: 1.129 ± 0.039
4.395AspIle: 4.395 ± 0.076
3.738AspLys: 3.738 ± 0.068
5.579AspLeu: 5.579 ± 0.087
1.216AspMet: 1.216 ± 0.038
2.816AspAsn: 2.816 ± 0.057
1.985AspPro: 1.985 ± 0.052
2.099AspGln: 2.099 ± 0.05
1.911AspArg: 1.911 ± 0.048
3.252AspSer: 3.252 ± 0.078
2.704AspThr: 2.704 ± 0.077
3.58AspVal: 3.58 ± 0.075
0.752AspTrp: 0.752 ± 0.029
2.087AspTyr: 2.087 ± 0.05
0.0AspXaa: 0.0 ± 0.0
Glu
4.7GluAla: 4.7 ± 0.088
0.494GluCys: 0.494 ± 0.026
2.846GluAsp: 2.846 ± 0.064
3.472GluGlu: 3.472 ± 0.08
2.525GluPhe: 2.525 ± 0.057
3.391GluGly: 3.391 ± 0.079
1.529GluHis: 1.529 ± 0.051
4.245GluIle: 4.245 ± 0.074
4.353GluLys: 4.353 ± 0.078
6.826GluLeu: 6.826 ± 0.085
1.673GluMet: 1.673 ± 0.047
3.29GluAsn: 3.29 ± 0.06
1.648GluPro: 1.648 ± 0.048
4.011GluGln: 4.011 ± 0.077
2.606GluArg: 2.606 ± 0.064
3.739GluSer: 3.739 ± 0.075
3.068GluThr: 3.068 ± 0.062
4.164GluVal: 4.164 ± 0.076
0.631GluTrp: 0.631 ± 0.025
1.836GluTyr: 1.836 ± 0.049
0.0GluXaa: 0.0 ± 0.0
Phe
3.515PheAla: 3.515 ± 0.069
0.56PheCys: 0.56 ± 0.024
2.832PheAsp: 2.832 ± 0.061
2.536PheGlu: 2.536 ± 0.056
1.872PhePhe: 1.872 ± 0.056
2.746PheGly: 2.746 ± 0.063
0.858PheHis: 0.858 ± 0.031
3.631PheIle: 3.631 ± 0.084
2.52PheLys: 2.52 ± 0.055
3.865PheLeu: 3.865 ± 0.079
1.008PheMet: 1.008 ± 0.031
2.394PheAsn: 2.394 ± 0.052
1.471PhePro: 1.471 ± 0.042
1.277PheGln: 1.277 ± 0.036
1.312PheArg: 1.312 ± 0.041
3.717PheSer: 3.717 ± 0.065
2.716PheThr: 2.716 ± 0.059
2.737PheVal: 2.737 ± 0.063
0.475PheTrp: 0.475 ± 0.023
1.494PheTyr: 1.494 ± 0.051
0.0PheXaa: 0.0 ± 0.0
Gly
4.818GlyAla: 4.818 ± 0.1
0.842GlyCys: 0.842 ± 0.028
3.464GlyAsp: 3.464 ± 0.077
4.05GlyGlu: 4.05 ± 0.083
3.109GlyPhe: 3.109 ± 0.058
4.328GlyGly: 4.328 ± 0.095
1.412GlyHis: 1.412 ± 0.049
4.915GlyIle: 4.915 ± 0.072
4.129GlyLys: 4.129 ± 0.079
6.671GlyLeu: 6.671 ± 0.101
1.698GlyMet: 1.698 ± 0.05
2.587GlyAsn: 2.587 ± 0.061
1.473GlyPro: 1.473 ± 0.045
2.536GlyGln: 2.536 ± 0.061
2.531GlyArg: 2.531 ± 0.062
3.799GlySer: 3.799 ± 0.077
3.305GlyThr: 3.305 ± 0.091
4.624GlyVal: 4.624 ± 0.079
0.77GlyTrp: 0.77 ± 0.03
2.427GlyTyr: 2.427 ± 0.056
0.0GlyXaa: 0.0 ± 0.0
His
1.457HisAla: 1.457 ± 0.044
0.368HisCys: 0.368 ± 0.019
1.058HisAsp: 1.058 ± 0.038
1.151HisGlu: 1.151 ± 0.044
1.26HisPhe: 1.26 ± 0.039
1.382HisGly: 1.382 ± 0.052
0.663HisHis: 0.663 ± 0.031
1.56HisIle: 1.56 ± 0.042
1.186HisLys: 1.186 ± 0.036
2.457HisLeu: 2.457 ± 0.057
0.416HisMet: 0.416 ± 0.022
1.012HisAsn: 1.012 ± 0.032
1.059HisPro: 1.059 ± 0.034
1.216HisGln: 1.216 ± 0.044
0.87HisArg: 0.87 ± 0.034
1.437HisSer: 1.437 ± 0.039
0.985HisThr: 0.985 ± 0.032
1.182HisVal: 1.182 ± 0.033
0.324HisTrp: 0.324 ± 0.02
0.88HisTyr: 0.88 ± 0.034
0.0HisXaa: 0.0 ± 0.0
Ile
6.996IleAla: 6.996 ± 0.09
0.812IleCys: 0.812 ± 0.034
4.792IleAsp: 4.792 ± 0.082
5.173IleGlu: 5.173 ± 0.085
2.834IlePhe: 2.834 ± 0.062
4.784IleGly: 4.784 ± 0.083
1.378IleHis: 1.378 ± 0.044
5.099IleIle: 5.099 ± 0.092
4.814IleLys: 4.814 ± 0.074
6.448IleLeu: 6.448 ± 0.101
1.411IleMet: 1.411 ± 0.039
4.013IleAsn: 4.013 ± 0.072
2.837IlePro: 2.837 ± 0.056
2.554IleGln: 2.554 ± 0.055
2.535IleArg: 2.535 ± 0.055
5.33IleSer: 5.33 ± 0.083
4.511IleThr: 4.511 ± 0.098
4.497IleVal: 4.497 ± 0.063
0.674IleTrp: 0.674 ± 0.029
2.112IleTyr: 2.112 ± 0.046
0.0IleXaa: 0.0 ± 0.0
Lys
5.059LysAla: 5.059 ± 0.084
0.394LysCys: 0.394 ± 0.022
3.098LysAsp: 3.098 ± 0.069
3.979LysGlu: 3.979 ± 0.077
1.866LysPhe: 1.866 ± 0.046
3.502LysGly: 3.502 ± 0.064
1.407LysHis: 1.407 ± 0.04
4.164LysIle: 4.164 ± 0.078
4.356LysLys: 4.356 ± 0.092
6.176LysLeu: 6.176 ± 0.098
1.677LysMet: 1.677 ± 0.04
3.258LysAsn: 3.258 ± 0.067
2.25LysPro: 2.25 ± 0.059
3.667LysGln: 3.667 ± 0.085
2.772LysArg: 2.772 ± 0.061
3.961LysSer: 3.961 ± 0.079
3.393LysThr: 3.393 ± 0.069
4.18LysVal: 4.18 ± 0.069
0.604LysTrp: 0.604 ± 0.025
1.636LysTyr: 1.636 ± 0.05
0.0LysXaa: 0.0 ± 0.0
Leu
9.23LeuAla: 9.23 ± 0.121
1.045LeuCys: 1.045 ± 0.035
5.628LeuAsp: 5.628 ± 0.081
5.551LeuGlu: 5.551 ± 0.087
4.874LeuPhe: 4.874 ± 0.097
6.505LeuGly: 6.505 ± 0.093
2.123LeuHis: 2.123 ± 0.059
7.796LeuIle: 7.796 ± 0.117
6.149LeuLys: 6.149 ± 0.089
11.817LeuLeu: 11.817 ± 0.18
2.595LeuMet: 2.595 ± 0.06
5.262LeuAsn: 5.262 ± 0.08
4.589LeuPro: 4.589 ± 0.082
4.502LeuGln: 4.502 ± 0.096
3.924LeuArg: 3.924 ± 0.07
8.214LeuSer: 8.214 ± 0.114
6.586LeuThr: 6.586 ± 0.098
6.537LeuVal: 6.537 ± 0.106
0.945LeuTrp: 0.945 ± 0.036
2.842LeuTyr: 2.842 ± 0.055
0.0LeuXaa: 0.0 ± 0.0
Met
2.078MetAla: 2.078 ± 0.047
0.179MetCys: 0.179 ± 0.013
1.165MetAsp: 1.165 ± 0.04
1.104MetGlu: 1.104 ± 0.039
0.901MetPhe: 0.901 ± 0.031
1.671MetGly: 1.671 ± 0.048
0.486MetHis: 0.486 ± 0.025
1.601MetIle: 1.601 ± 0.043
1.535MetLys: 1.535 ± 0.045
2.642MetLeu: 2.642 ± 0.06
0.674MetMet: 0.674 ± 0.031
1.131MetAsn: 1.131 ± 0.036
1.148MetPro: 1.148 ± 0.036
1.186MetGln: 1.186 ± 0.043
1.034MetArg: 1.034 ± 0.043
1.813MetSer: 1.813 ± 0.044
1.429MetThr: 1.429 ± 0.043
1.577MetVal: 1.577 ± 0.046
0.199MetTrp: 0.199 ± 0.015
0.463MetTyr: 0.463 ± 0.024
0.0MetXaa: 0.0 ± 0.0
Asn
3.646AsnAla: 3.646 ± 0.059
0.475AsnCys: 0.475 ± 0.022
2.687AsnAsp: 2.687 ± 0.066
2.938AsnGlu: 2.938 ± 0.06
1.837AsnPhe: 1.837 ± 0.048
2.914AsnGly: 2.914 ± 0.07
1.025AsnHis: 1.025 ± 0.04
3.899AsnIle: 3.899 ± 0.056
3.655AsnLys: 3.655 ± 0.075
4.457AsnLeu: 4.457 ± 0.069
1.048AsnMet: 1.048 ± 0.036
2.944AsnAsn: 2.944 ± 0.055
1.954AsnPro: 1.954 ± 0.048
2.091AsnGln: 2.091 ± 0.052
1.719AsnArg: 1.719 ± 0.048
3.112AsnSer: 3.112 ± 0.057
2.693AsnThr: 2.693 ± 0.061
2.82AsnVal: 2.82 ± 0.07
0.596AsnTrp: 0.596 ± 0.025
1.56AsnTyr: 1.56 ± 0.054
0.0AsnXaa: 0.0 ± 0.0
Pro
2.766ProAla: 2.766 ± 0.061
0.316ProCys: 0.316 ± 0.018
2.028ProAsp: 2.028 ± 0.054
3.073ProGlu: 3.073 ± 0.064
1.714ProPhe: 1.714 ± 0.043
1.964ProGly: 1.964 ± 0.051
0.761ProHis: 0.761 ± 0.029
2.771ProIle: 2.771 ± 0.055
2.007ProLys: 2.007 ± 0.057
3.894ProLeu: 3.894 ± 0.062
0.861ProMet: 0.861 ± 0.032
1.617ProAsn: 1.617 ± 0.04
0.957ProPro: 0.957 ± 0.038
1.381ProGln: 1.381 ± 0.041
1.161ProArg: 1.161 ± 0.037
2.335ProSer: 2.335 ± 0.056
2.125ProThr: 2.125 ± 0.052
2.632ProVal: 2.632 ± 0.057
0.427ProTrp: 0.427 ± 0.023
1.205ProTyr: 1.205 ± 0.039
0.0ProXaa: 0.0 ± 0.0
Gln
4.038GlnAla: 4.038 ± 0.07
0.418GlnCys: 0.418 ± 0.023
1.952GlnAsp: 1.952 ± 0.046
2.464GlnGlu: 2.464 ± 0.056
1.773GlnPhe: 1.773 ± 0.048
2.771GlnGly: 2.771 ± 0.06
1.264GlnHis: 1.264 ± 0.041
2.747GlnIle: 2.747 ± 0.067
2.767GlnLys: 2.767 ± 0.068
5.644GlnLeu: 5.644 ± 0.107
0.985GlnMet: 0.985 ± 0.042
1.735GlnAsn: 1.735 ± 0.049
1.493GlnPro: 1.493 ± 0.043
3.583GlnGln: 3.583 ± 0.101
2.046GlnArg: 2.046 ± 0.055
2.77GlnSer: 2.77 ± 0.057
2.372GlnThr: 2.372 ± 0.056
3.143GlnVal: 3.143 ± 0.068
0.603GlnTrp: 0.603 ± 0.027
1.48GlnTyr: 1.48 ± 0.037
0.0GlnXaa: 0.0 ± 0.0
Arg
2.67ArgAla: 2.67 ± 0.055
0.422ArgCys: 0.422 ± 0.021
2.047ArgAsp: 2.047 ± 0.058
2.402ArgGlu: 2.402 ± 0.06
1.981ArgPhe: 1.981 ± 0.052
2.161ArgGly: 2.161 ± 0.053
0.922ArgHis: 0.922 ± 0.034
2.856ArgIle: 2.856 ± 0.06
2.125ArgLys: 2.125 ± 0.053
4.411ArgLeu: 4.411 ± 0.078
0.892ArgMet: 0.892 ± 0.031
1.693ArgAsn: 1.693 ± 0.044
1.353ArgPro: 1.353 ± 0.046
1.692ArgGln: 1.692 ± 0.051
1.735ArgArg: 1.735 ± 0.054
2.354ArgSer: 2.354 ± 0.049
1.866ArgThr: 1.866 ± 0.053
2.591ArgVal: 2.591 ± 0.055
0.49ArgTrp: 0.49 ± 0.023
1.544ArgTyr: 1.544 ± 0.041
0.0ArgXaa: 0.0 ± 0.0
Ser
5.133SerAla: 5.133 ± 0.078
0.643SerCys: 0.643 ± 0.031
3.782SerAsp: 3.782 ± 0.094
4.177SerGlu: 4.177 ± 0.076
3.207SerPhe: 3.207 ± 0.061
4.678SerGly: 4.678 ± 0.08
1.532SerHis: 1.532 ± 0.042
5.024SerIle: 5.024 ± 0.078
3.657SerLys: 3.657 ± 0.071
7.399SerLeu: 7.399 ± 0.111
1.575SerMet: 1.575 ± 0.042
3.073SerAsn: 3.073 ± 0.068
2.331SerPro: 2.331 ± 0.049
2.862SerGln: 2.862 ± 0.056
2.554SerArg: 2.554 ± 0.056
4.448SerSer: 4.448 ± 0.092
3.499SerThr: 3.499 ± 0.067
4.498SerVal: 4.498 ± 0.08
0.722SerTrp: 0.722 ± 0.031
2.069SerTyr: 2.069 ± 0.048
0.0SerXaa: 0.0 ± 0.0
Thr
4.422ThrAla: 4.422 ± 0.08
0.513ThrCys: 0.513 ± 0.025
3.053ThrAsp: 3.053 ± 0.074
3.485ThrGlu: 3.485 ± 0.074
2.319ThrPhe: 2.319 ± 0.057
4.059ThrGly: 4.059 ± 0.077
1.275ThrHis: 1.275 ± 0.035
3.955ThrIle: 3.955 ± 0.076
2.704ThrLys: 2.704 ± 0.06
6.626ThrLeu: 6.626 ± 0.093
1.135ThrMet: 1.135 ± 0.035
2.369ThrAsn: 2.369 ± 0.06
2.618ThrPro: 2.618 ± 0.06
2.468ThrGln: 2.468 ± 0.053
1.966ThrArg: 1.966 ± 0.054
3.392ThrSer: 3.392 ± 0.076
3.216ThrThr: 3.216 ± 0.075
3.695ThrVal: 3.695 ± 0.09
0.61ThrTrp: 0.61 ± 0.026
1.614ThrTyr: 1.614 ± 0.047
0.0ThrXaa: 0.0 ± 0.0
Val
5.584ValAla: 5.584 ± 0.084
0.697ValCys: 0.697 ± 0.031
4.033ValAsp: 4.033 ± 0.087
4.301ValGlu: 4.301 ± 0.083
2.858ValPhe: 2.858 ± 0.069
4.194ValGly: 4.194 ± 0.081
1.208ValHis: 1.208 ± 0.037
5.159ValIle: 5.159 ± 0.073
3.81ValLys: 3.81 ± 0.068
6.589ValLeu: 6.589 ± 0.101
1.691ValMet: 1.691 ± 0.042
3.264ValAsn: 3.264 ± 0.061
2.201ValPro: 2.201 ± 0.053
2.157ValGln: 2.157 ± 0.056
2.285ValArg: 2.285 ± 0.053
4.623ValSer: 4.623 ± 0.085
3.961ValThr: 3.961 ± 0.104
4.726ValVal: 4.726 ± 0.083
0.609ValTrp: 0.609 ± 0.03
1.86ValTyr: 1.86 ± 0.047
0.0ValXaa: 0.0 ± 0.0
Trp
0.769TrpAla: 0.769 ± 0.027
0.15TrpCys: 0.15 ± 0.014
0.536TrpAsp: 0.536 ± 0.022
0.484TrpGlu: 0.484 ± 0.021
0.551TrpPhe: 0.551 ± 0.024
0.716TrpGly: 0.716 ± 0.026
0.277TrpHis: 0.277 ± 0.015
0.716TrpIle: 0.716 ± 0.034
0.536TrpLys: 0.536 ± 0.027
1.495TrpLeu: 1.495 ± 0.046
0.283TrpMet: 0.283 ± 0.019
0.428TrpAsn: 0.428 ± 0.023
0.417TrpPro: 0.417 ± 0.023
0.73TrpGln: 0.73 ± 0.031
0.479TrpArg: 0.479 ± 0.024
0.715TrpSer: 0.715 ± 0.033
0.464TrpThr: 0.464 ± 0.021
0.764TrpVal: 0.764 ± 0.029
0.145TrpTrp: 0.145 ± 0.012
0.346TrpTyr: 0.346 ± 0.021
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.239TyrAla: 2.239 ± 0.053
0.427TyrCys: 0.427 ± 0.025
1.611TyrAsp: 1.611 ± 0.05
1.56TyrGlu: 1.56 ± 0.044
1.654TyrPhe: 1.654 ± 0.044
1.991TyrGly: 1.991 ± 0.047
0.788TyrHis: 0.788 ± 0.033
2.024TyrIle: 2.024 ± 0.054
1.694TyrLys: 1.694 ± 0.05
3.547TyrLeu: 3.547 ± 0.066
0.682TyrMet: 0.682 ± 0.03
1.273TyrAsn: 1.273 ± 0.038
1.335TyrPro: 1.335 ± 0.041
2.081TyrGln: 2.081 ± 0.055
1.46TyrArg: 1.46 ± 0.038
2.132TyrSer: 2.132 ± 0.055
1.562TyrThr: 1.562 ± 0.045
1.728TyrVal: 1.728 ± 0.041
0.431TyrTrp: 0.431 ± 0.023
1.108TyrTyr: 1.108 ± 0.04
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2647 proteins (880803 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski