Bacteriophage R18C

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Peduovirinae; Peduovirus; Citrobacter virus R18C

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 44 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5P1MAT4|A0A5P1MAT4_9CAUD Baseplate assembly protein J OS=Bacteriophage R18C OX=2592161 PE=4 SV=1
MM1 pKa = 7.75ANNLFITYY9 pKa = 10.49DD10 pKa = 4.07LIKK13 pKa = 9.6TKK15 pKa = 10.5DD16 pKa = 3.38YY17 pKa = 11.16AAVYY21 pKa = 9.98DD22 pKa = 4.68AIKK25 pKa = 10.96SLGNWALTTEE35 pKa = 4.52SNWYY39 pKa = 9.18VNCSYY44 pKa = 10.76SAEE47 pKa = 4.06DD48 pKa = 2.99AAKK51 pKa = 10.02IVRR54 pKa = 11.84AVMDD58 pKa = 4.39SDD60 pKa = 4.46DD61 pKa = 3.94KK62 pKa = 11.71LIVVDD67 pKa = 3.85ATNNSAYY74 pKa = 9.35WYY76 pKa = 9.12NLSDD80 pKa = 3.59EE81 pKa = 4.53VSNQILTEE89 pKa = 4.08WNKK92 pKa = 10.76

Molecular weight: 10.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5P1MBE0|A0A5P1MBE0_9CAUD C protein OS=Bacteriophage R18C OX=2592161 PE=4 SV=1
MM1 pKa = 7.44NEE3 pKa = 4.18FKK5 pKa = 10.81RR6 pKa = 11.84FEE8 pKa = 4.32DD9 pKa = 3.63RR10 pKa = 11.84LTGLIEE16 pKa = 4.13SLSPSGRR23 pKa = 11.84RR24 pKa = 11.84RR25 pKa = 11.84LAVDD29 pKa = 3.28IAKK32 pKa = 10.44KK33 pKa = 10.15LRR35 pKa = 11.84QRR37 pKa = 11.84QQQRR41 pKa = 11.84IKK43 pKa = 10.31LQKK46 pKa = 10.67GPDD49 pKa = 3.48GTPYY53 pKa = 10.34VPRR56 pKa = 11.84KK57 pKa = 8.21NQPVRR62 pKa = 11.84NKK64 pKa = 9.61KK65 pKa = 10.05GRR67 pKa = 11.84IKK69 pKa = 10.81RR70 pKa = 11.84EE71 pKa = 3.77MFVKK75 pKa = 10.46LRR77 pKa = 11.84TNRR80 pKa = 11.84FMKK83 pKa = 10.1ATGSEE88 pKa = 4.08SAAVVEE94 pKa = 4.94FASGVQRR101 pKa = 11.84IARR104 pKa = 11.84VHH106 pKa = 5.0QLGLKK111 pKa = 9.89DD112 pKa = 3.44KK113 pKa = 9.84PGRR116 pKa = 11.84NSAVVEE122 pKa = 4.17YY123 pKa = 9.45PVRR126 pKa = 11.84EE127 pKa = 3.78LFGFDD132 pKa = 3.68KK133 pKa = 10.99EE134 pKa = 4.59SIQLIEE140 pKa = 4.62RR141 pKa = 11.84EE142 pKa = 4.16LLVILSKK149 pKa = 11.15DD150 pKa = 3.72VII152 pKa = 4.08

Molecular weight: 17.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

44

0

44

9649

44

815

219.3

24.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.011 ± 0.682

1.016 ± 0.141

5.628 ± 0.23

6.208 ± 0.301

3.503 ± 0.288

6.374 ± 0.398

1.907 ± 0.224

5.482 ± 0.257

5.938 ± 0.39

8.934 ± 0.281

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.705 ± 0.17

4.197 ± 0.254

4.052 ± 0.29

3.793 ± 0.213

6.529 ± 0.386

6.001 ± 0.212

6.55 ± 0.449

6.778 ± 0.32

1.482 ± 0.146

2.912 ± 0.192
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski