Amino acid dipepetide frequency for Bacteriophage R18C

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.987AlaAla: 10.987 ± 2.197
1.14AlaCys: 1.14 ± 0.401
5.701AlaAsp: 5.701 ± 0.894
5.804AlaGlu: 5.804 ± 0.734
3.317AlaPhe: 3.317 ± 0.55
8.085AlaGly: 8.085 ± 1.16
1.658AlaHis: 1.658 ± 0.353
4.042AlaIle: 4.042 ± 0.619
4.975AlaLys: 4.975 ± 0.742
10.676AlaLeu: 10.676 ± 1.211
1.866AlaMet: 1.866 ± 0.424
2.591AlaAsn: 2.591 ± 0.461
4.25AlaPro: 4.25 ± 0.692
3.42AlaGln: 3.42 ± 0.724
4.871AlaArg: 4.871 ± 0.93
7.67AlaSer: 7.67 ± 0.988
6.944AlaThr: 6.944 ± 0.894
7.981AlaVal: 7.981 ± 0.962
1.762AlaTrp: 1.762 ± 0.397
2.384AlaTyr: 2.384 ± 0.437
0.0AlaXaa: 0.0 ± 0.0
Cys
0.726CysAla: 0.726 ± 0.226
0.0CysCys: 0.0 ± 0.0
0.829CysAsp: 0.829 ± 0.28
0.311CysGlu: 0.311 ± 0.199
0.311CysPhe: 0.311 ± 0.169
0.726CysGly: 0.726 ± 0.263
0.207CysHis: 0.207 ± 0.146
0.622CysIle: 0.622 ± 0.282
0.415CysLys: 0.415 ± 0.197
0.622CysLeu: 0.622 ± 0.262
0.311CysMet: 0.311 ± 0.189
0.207CysAsn: 0.207 ± 0.131
0.415CysPro: 0.415 ± 0.208
0.829CysGln: 0.829 ± 0.317
1.036CysArg: 1.036 ± 0.294
0.518CysSer: 0.518 ± 0.232
0.829CysThr: 0.829 ± 0.325
0.726CysVal: 0.726 ± 0.279
0.104CysTrp: 0.104 ± 0.095
0.415CysTyr: 0.415 ± 0.226
0.0CysXaa: 0.0 ± 0.0
Asp
7.152AspAla: 7.152 ± 0.906
0.518AspCys: 0.518 ± 0.219
3.213AspAsp: 3.213 ± 0.598
4.25AspGlu: 4.25 ± 0.815
2.695AspPhe: 2.695 ± 0.732
4.871AspGly: 4.871 ± 0.638
0.415AspHis: 0.415 ± 0.196
4.457AspIle: 4.457 ± 0.93
2.902AspLys: 2.902 ± 0.489
4.353AspLeu: 4.353 ± 0.61
0.829AspMet: 0.829 ± 0.299
1.866AspAsn: 1.866 ± 0.392
1.555AspPro: 1.555 ± 0.42
1.451AspGln: 1.451 ± 0.5
2.177AspArg: 2.177 ± 0.48
3.109AspSer: 3.109 ± 0.529
3.731AspThr: 3.731 ± 0.653
3.628AspVal: 3.628 ± 0.533
0.829AspTrp: 0.829 ± 0.255
2.28AspTyr: 2.28 ± 0.536
0.0AspXaa: 0.0 ± 0.0
Glu
4.768GluAla: 4.768 ± 0.752
0.415GluCys: 0.415 ± 0.177
1.969GluAsp: 1.969 ± 0.451
3.213GluGlu: 3.213 ± 0.474
2.177GluPhe: 2.177 ± 0.561
2.384GluGly: 2.384 ± 0.507
1.555GluHis: 1.555 ± 0.389
2.384GluIle: 2.384 ± 0.571
4.561GluLys: 4.561 ± 0.843
7.566GluLeu: 7.566 ± 0.631
2.591GluMet: 2.591 ± 0.41
3.213GluAsn: 3.213 ± 0.627
2.591GluPro: 2.591 ± 0.58
2.799GluGln: 2.799 ± 0.55
4.768GluArg: 4.768 ± 0.973
4.353GluSer: 4.353 ± 0.822
3.42GluThr: 3.42 ± 0.638
3.524GluVal: 3.524 ± 0.64
1.14GluTrp: 1.14 ± 0.326
2.695GluTyr: 2.695 ± 0.512
0.0GluXaa: 0.0 ± 0.0
Phe
2.799PheAla: 2.799 ± 0.458
0.518PheCys: 0.518 ± 0.213
1.866PheAsp: 1.866 ± 0.346
2.488PheGlu: 2.488 ± 0.553
1.555PhePhe: 1.555 ± 0.608
1.451PheGly: 1.451 ± 0.334
0.933PheHis: 0.933 ± 0.327
1.451PheIle: 1.451 ± 0.395
2.695PheLys: 2.695 ± 0.491
3.524PheLeu: 3.524 ± 0.623
0.829PheMet: 0.829 ± 0.321
1.866PheAsn: 1.866 ± 0.351
1.244PhePro: 1.244 ± 0.333
1.555PheGln: 1.555 ± 0.332
1.866PheArg: 1.866 ± 0.393
2.591PheSer: 2.591 ± 0.513
2.695PheThr: 2.695 ± 0.449
1.244PheVal: 1.244 ± 0.304
0.829PheTrp: 0.829 ± 0.276
1.036PheTyr: 1.036 ± 0.375
0.0PheXaa: 0.0 ± 0.0
Gly
4.975GlyAla: 4.975 ± 0.993
0.933GlyCys: 0.933 ± 0.362
5.39GlyAsp: 5.39 ± 0.853
3.731GlyGlu: 3.731 ± 0.61
2.28GlyPhe: 2.28 ± 0.563
4.871GlyGly: 4.871 ± 0.933
0.933GlyHis: 0.933 ± 0.208
3.109GlyIle: 3.109 ± 0.582
4.975GlyLys: 4.975 ± 0.6
4.457GlyLeu: 4.457 ± 0.691
2.177GlyMet: 2.177 ± 0.661
2.591GlyAsn: 2.591 ± 0.447
0.311GlyPro: 0.311 ± 0.182
2.28GlyGln: 2.28 ± 0.544
4.664GlyArg: 4.664 ± 0.735
3.628GlySer: 3.628 ± 0.565
4.146GlyThr: 4.146 ± 0.924
5.597GlyVal: 5.597 ± 0.879
0.933GlyTrp: 0.933 ± 0.212
1.762GlyTyr: 1.762 ± 0.382
0.0GlyXaa: 0.0 ± 0.0
His
1.969HisAla: 1.969 ± 0.541
0.415HisCys: 0.415 ± 0.197
1.14HisAsp: 1.14 ± 0.346
1.036HisGlu: 1.036 ± 0.35
0.622HisPhe: 0.622 ± 0.297
1.244HisGly: 1.244 ± 0.488
0.622HisHis: 0.622 ± 0.234
1.555HisIle: 1.555 ± 0.403
0.829HisLys: 0.829 ± 0.323
1.658HisLeu: 1.658 ± 0.392
0.415HisMet: 0.415 ± 0.189
0.933HisAsn: 0.933 ± 0.436
1.244HisPro: 1.244 ± 0.34
0.933HisGln: 0.933 ± 0.34
1.14HisArg: 1.14 ± 0.346
0.726HisSer: 0.726 ± 0.248
0.829HisThr: 0.829 ± 0.293
0.829HisVal: 0.829 ± 0.286
0.311HisTrp: 0.311 ± 0.192
0.622HisTyr: 0.622 ± 0.231
0.0HisXaa: 0.0 ± 0.0
Ile
5.286IleAla: 5.286 ± 0.781
0.415IleCys: 0.415 ± 0.222
2.695IleAsp: 2.695 ± 0.666
3.524IleGlu: 3.524 ± 0.547
1.866IlePhe: 1.866 ± 0.581
3.524IleGly: 3.524 ± 0.697
0.622IleHis: 0.622 ± 0.269
3.109IleIle: 3.109 ± 0.678
2.902IleLys: 2.902 ± 0.784
3.109IleLeu: 3.109 ± 0.637
0.933IleMet: 0.933 ± 0.278
3.109IleAsn: 3.109 ± 0.505
2.591IlePro: 2.591 ± 0.641
1.866IleGln: 1.866 ± 0.463
4.457IleArg: 4.457 ± 0.535
4.25IleSer: 4.25 ± 0.576
4.768IleThr: 4.768 ± 0.649
3.524IleVal: 3.524 ± 0.595
0.829IleTrp: 0.829 ± 0.246
1.451IleTyr: 1.451 ± 0.402
0.0IleXaa: 0.0 ± 0.0
Lys
5.701LysAla: 5.701 ± 0.671
0.311LysCys: 0.311 ± 0.167
2.073LysAsp: 2.073 ± 0.469
3.524LysGlu: 3.524 ± 0.707
1.969LysPhe: 1.969 ± 0.412
3.109LysGly: 3.109 ± 0.468
1.762LysHis: 1.762 ± 0.477
2.591LysIle: 2.591 ± 0.494
4.975LysLys: 4.975 ± 1.117
6.219LysLeu: 6.219 ± 0.917
0.726LysMet: 0.726 ± 0.31
4.25LysAsn: 4.25 ± 0.82
2.902LysPro: 2.902 ± 0.595
1.658LysGln: 1.658 ± 0.4
4.042LysArg: 4.042 ± 0.707
2.695LysSer: 2.695 ± 0.556
3.628LysThr: 3.628 ± 0.673
3.628LysVal: 3.628 ± 0.571
1.244LysTrp: 1.244 ± 0.401
2.384LysTyr: 2.384 ± 0.456
0.0LysXaa: 0.0 ± 0.0
Leu
10.158LeuAla: 10.158 ± 1.072
0.829LeuCys: 0.829 ± 0.24
4.457LeuAsp: 4.457 ± 0.852
5.701LeuGlu: 5.701 ± 0.792
4.042LeuPhe: 4.042 ± 0.901
4.353LeuGly: 4.353 ± 0.704
1.555LeuHis: 1.555 ± 0.444
4.871LeuIle: 4.871 ± 0.66
5.701LeuLys: 5.701 ± 0.844
5.597LeuLeu: 5.597 ± 1.005
3.628LeuMet: 3.628 ± 0.511
4.561LeuAsn: 4.561 ± 0.609
3.835LeuPro: 3.835 ± 0.584
2.902LeuGln: 2.902 ± 0.574
5.286LeuArg: 5.286 ± 0.555
6.426LeuSer: 6.426 ± 0.826
7.463LeuThr: 7.463 ± 0.917
4.353LeuVal: 4.353 ± 0.539
1.14LeuTrp: 1.14 ± 0.313
2.384LeuTyr: 2.384 ± 0.648
0.0LeuXaa: 0.0 ± 0.0
Met
3.213MetAla: 3.213 ± 0.503
0.207MetCys: 0.207 ± 0.152
1.244MetAsp: 1.244 ± 0.422
1.244MetGlu: 1.244 ± 0.326
0.933MetPhe: 0.933 ± 0.291
1.036MetGly: 1.036 ± 0.285
0.622MetHis: 0.622 ± 0.245
1.036MetIle: 1.036 ± 0.332
1.347MetLys: 1.347 ± 0.353
2.488MetLeu: 2.488 ± 0.624
1.036MetMet: 1.036 ± 0.34
1.658MetAsn: 1.658 ± 0.461
1.244MetPro: 1.244 ± 0.34
0.726MetGln: 0.726 ± 0.274
2.073MetArg: 2.073 ± 0.557
1.658MetSer: 1.658 ± 0.313
3.006MetThr: 3.006 ± 0.535
1.451MetVal: 1.451 ± 0.326
0.311MetTrp: 0.311 ± 0.18
0.518MetTyr: 0.518 ± 0.208
0.0MetXaa: 0.0 ± 0.0
Asn
3.42AsnAla: 3.42 ± 0.681
0.415AsnCys: 0.415 ± 0.19
2.28AsnAsp: 2.28 ± 0.703
2.591AsnGlu: 2.591 ± 0.541
1.347AsnPhe: 1.347 ± 0.364
4.042AsnGly: 4.042 ± 0.831
1.036AsnHis: 1.036 ± 0.42
2.902AsnIle: 2.902 ± 0.48
2.799AsnLys: 2.799 ± 0.597
3.109AsnLeu: 3.109 ± 0.547
1.036AsnMet: 1.036 ± 0.321
1.555AsnAsn: 1.555 ± 0.319
1.866AsnPro: 1.866 ± 0.481
1.451AsnGln: 1.451 ± 0.388
3.006AsnArg: 3.006 ± 0.563
3.109AsnSer: 3.109 ± 0.638
2.384AsnThr: 2.384 ± 0.588
1.969AsnVal: 1.969 ± 0.321
0.415AsnTrp: 0.415 ± 0.262
1.244AsnTyr: 1.244 ± 0.353
0.0AsnXaa: 0.0 ± 0.0
Pro
3.939ProAla: 3.939 ± 0.699
0.207ProCys: 0.207 ± 0.142
3.42ProAsp: 3.42 ± 0.655
2.902ProGlu: 2.902 ± 0.563
1.244ProPhe: 1.244 ± 0.495
2.384ProGly: 2.384 ± 0.516
1.244ProHis: 1.244 ± 0.425
1.866ProIle: 1.866 ± 0.468
2.28ProLys: 2.28 ± 0.577
3.628ProLeu: 3.628 ± 0.51
0.518ProMet: 0.518 ± 0.209
0.726ProAsn: 0.726 ± 0.312
1.866ProPro: 1.866 ± 0.492
1.658ProGln: 1.658 ± 0.462
2.384ProArg: 2.384 ± 0.703
2.28ProSer: 2.28 ± 0.672
1.658ProThr: 1.658 ± 0.376
5.079ProVal: 5.079 ± 0.79
0.518ProTrp: 0.518 ± 0.219
0.726ProTyr: 0.726 ± 0.303
0.0ProXaa: 0.0 ± 0.0
Gln
3.628GlnAla: 3.628 ± 1.208
0.207GlnCys: 0.207 ± 0.152
1.969GlnAsp: 1.969 ± 0.487
2.384GlnGlu: 2.384 ± 0.511
0.518GlnPhe: 0.518 ± 0.176
1.451GlnGly: 1.451 ± 0.321
0.622GlnHis: 0.622 ± 0.275
2.488GlnIle: 2.488 ± 0.698
2.591GlnLys: 2.591 ± 0.431
4.25GlnLeu: 4.25 ± 0.566
0.933GlnMet: 0.933 ± 0.242
1.14GlnAsn: 1.14 ± 0.403
1.866GlnPro: 1.866 ± 0.534
2.073GlnGln: 2.073 ± 0.558
4.457GlnArg: 4.457 ± 0.771
1.969GlnSer: 1.969 ± 0.49
1.969GlnThr: 1.969 ± 0.401
2.177GlnVal: 2.177 ± 0.468
0.518GlnTrp: 0.518 ± 0.252
0.726GlnTyr: 0.726 ± 0.247
0.0GlnXaa: 0.0 ± 0.0
Arg
6.115ArgAla: 6.115 ± 0.76
0.933ArgCys: 0.933 ± 0.262
3.317ArgAsp: 3.317 ± 0.435
4.975ArgGlu: 4.975 ± 0.749
1.866ArgPhe: 1.866 ± 0.427
3.524ArgGly: 3.524 ± 0.873
1.244ArgHis: 1.244 ± 0.366
3.731ArgIle: 3.731 ± 0.766
3.213ArgLys: 3.213 ± 0.456
5.597ArgLeu: 5.597 ± 0.676
1.451ArgMet: 1.451 ± 0.462
3.317ArgAsn: 3.317 ± 0.614
2.177ArgPro: 2.177 ± 0.347
3.731ArgGln: 3.731 ± 0.805
4.871ArgArg: 4.871 ± 0.763
3.006ArgSer: 3.006 ± 0.54
3.109ArgThr: 3.109 ± 0.497
5.079ArgVal: 5.079 ± 0.985
1.036ArgTrp: 1.036 ± 0.354
3.006ArgTyr: 3.006 ± 0.555
0.0ArgXaa: 0.0 ± 0.0
Ser
5.908SerAla: 5.908 ± 0.988
0.518SerCys: 0.518 ± 0.209
3.628SerAsp: 3.628 ± 0.582
3.628SerGlu: 3.628 ± 0.629
1.762SerPhe: 1.762 ± 0.514
3.731SerGly: 3.731 ± 0.589
1.036SerHis: 1.036 ± 0.559
3.006SerIle: 3.006 ± 0.608
3.731SerLys: 3.731 ± 0.601
6.841SerLeu: 6.841 ± 1.054
1.658SerMet: 1.658 ± 0.467
2.384SerAsn: 2.384 ± 0.515
2.384SerPro: 2.384 ± 0.623
2.488SerGln: 2.488 ± 0.503
4.042SerArg: 4.042 ± 0.602
2.488SerSer: 2.488 ± 0.599
4.042SerThr: 4.042 ± 0.631
4.975SerVal: 4.975 ± 0.747
0.518SerTrp: 0.518 ± 0.245
1.244SerTyr: 1.244 ± 0.399
0.0SerXaa: 0.0 ± 0.0
Thr
7.877ThrAla: 7.877 ± 1.361
0.829ThrCys: 0.829 ± 0.318
3.731ThrAsp: 3.731 ± 0.492
3.006ThrGlu: 3.006 ± 0.573
2.28ThrPhe: 2.28 ± 0.519
6.426ThrGly: 6.426 ± 0.945
0.933ThrHis: 0.933 ± 0.284
3.317ThrIle: 3.317 ± 0.56
3.006ThrLys: 3.006 ± 0.75
7.255ThrLeu: 7.255 ± 1.077
2.384ThrMet: 2.384 ± 0.429
1.969ThrAsn: 1.969 ± 0.593
3.317ThrPro: 3.317 ± 0.471
1.762ThrGln: 1.762 ± 0.455
4.146ThrArg: 4.146 ± 0.679
2.902ThrSer: 2.902 ± 0.371
4.042ThrThr: 4.042 ± 0.897
4.664ThrVal: 4.664 ± 0.826
0.518ThrTrp: 0.518 ± 0.208
1.14ThrTyr: 1.14 ± 0.372
0.0ThrXaa: 0.0 ± 0.0
Val
7.152ValAla: 7.152 ± 0.933
1.244ValCys: 1.244 ± 0.37
4.664ValAsp: 4.664 ± 0.629
4.353ValGlu: 4.353 ± 0.743
2.591ValPhe: 2.591 ± 0.523
4.25ValGly: 4.25 ± 0.74
0.829ValHis: 0.829 ± 0.3
5.079ValIle: 5.079 ± 0.675
4.353ValLys: 4.353 ± 0.719
5.079ValLeu: 5.079 ± 0.891
2.073ValMet: 2.073 ± 0.512
2.488ValAsn: 2.488 ± 0.593
2.488ValPro: 2.488 ± 0.484
2.177ValGln: 2.177 ± 0.418
2.591ValArg: 2.591 ± 0.567
4.457ValSer: 4.457 ± 0.533
5.079ValThr: 5.079 ± 0.91
5.079ValVal: 5.079 ± 0.69
0.415ValTrp: 0.415 ± 0.193
1.347ValTyr: 1.347 ± 0.324
0.0ValXaa: 0.0 ± 0.0
Trp
1.555TrpAla: 1.555 ± 0.297
0.0TrpCys: 0.0 ± 0.0
0.933TrpAsp: 0.933 ± 0.251
0.829TrpGlu: 0.829 ± 0.22
0.311TrpPhe: 0.311 ± 0.16
0.622TrpGly: 0.622 ± 0.297
0.518TrpHis: 0.518 ± 0.261
0.622TrpIle: 0.622 ± 0.242
0.726TrpLys: 0.726 ± 0.284
1.658TrpLeu: 1.658 ± 0.481
0.415TrpMet: 0.415 ± 0.203
0.518TrpAsn: 0.518 ± 0.253
1.036TrpPro: 1.036 ± 0.389
0.311TrpGln: 0.311 ± 0.15
1.658TrpArg: 1.658 ± 0.471
0.622TrpSer: 0.622 ± 0.244
0.311TrpThr: 0.311 ± 0.161
0.518TrpVal: 0.518 ± 0.202
0.622TrpTrp: 0.622 ± 0.223
1.036TrpTyr: 1.036 ± 0.301
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.799TyrAla: 2.799 ± 0.634
0.104TyrCys: 0.104 ± 0.088
1.762TyrAsp: 1.762 ± 0.473
2.591TyrGlu: 2.591 ± 0.528
1.347TyrPhe: 1.347 ± 0.381
1.658TyrGly: 1.658 ± 0.442
0.726TyrHis: 0.726 ± 0.206
2.591TyrIle: 2.591 ± 0.552
0.415TyrLys: 0.415 ± 0.179
1.658TyrLeu: 1.658 ± 0.386
1.14TyrMet: 1.14 ± 0.323
1.036TyrAsn: 1.036 ± 0.346
1.347TyrPro: 1.347 ± 0.418
1.866TyrGln: 1.866 ± 0.471
1.762TyrArg: 1.762 ± 0.508
1.555TyrSer: 1.555 ± 0.384
1.451TyrThr: 1.451 ± 0.428
1.762TyrVal: 1.762 ± 0.412
0.829TyrTrp: 0.829 ± 0.275
0.726TyrTyr: 0.726 ± 0.266
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 44 proteins (9649 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski