Pogona vitticeps (central bearded dragon)
Average proteome isoelectric point is 6.58
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 31185 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6J0V846|A0A6J0V846_9SAUR ninein OS=Pogona vitticeps OX=103695 GN=NIN PE=4 SV=1
MM1 pKa = 8.24 DD2 pKa = 4.75 FQQLADD8 pKa = 5.0 VADD11 pKa = 4.29 KK12 pKa = 10.12 WCSSNPFEE20 pKa = 6.07 LIATEE25 pKa = 3.96 EE26 pKa = 4.32 TEE28 pKa = 4.33 RR29 pKa = 11.84 RR30 pKa = 11.84 MDD32 pKa = 4.29 FYY34 pKa = 11.47 ADD36 pKa = 3.74 PGVSFYY42 pKa = 10.98 VLCPDD47 pKa = 4.64 NGCGDD52 pKa = 3.96 NFHH55 pKa = 6.3 VWSEE59 pKa = 4.48 SEE61 pKa = 3.97 DD62 pKa = 3.63 CLPFLQLAQDD72 pKa = 4.45 YY73 pKa = 10.71 ISSCGKK79 pKa = 8.61 KK80 pKa = 7.74 TLHH83 pKa = 6.85 EE84 pKa = 4.21 ILEE87 pKa = 4.3 KK88 pKa = 10.47 VLRR91 pKa = 11.84 SFRR94 pKa = 11.84 PLLGLPDD101 pKa = 3.71 VDD103 pKa = 4.94 DD104 pKa = 5.38 DD105 pKa = 5.61 AFEE108 pKa = 4.61 EE109 pKa = 4.49 YY110 pKa = 10.57 NADD113 pKa = 3.67 VEE115 pKa = 4.42 EE116 pKa = 4.81 EE117 pKa = 4.01 EE118 pKa = 5.78 AEE120 pKa = 4.39 ADD122 pKa = 3.72 HH123 pKa = 6.16 QQMGVSQQQ131 pKa = 2.96
Molecular weight: 14.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.761
IPC2_protein 3.91
IPC_protein 3.884
Toseland 3.681
ProMoST 4.024
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.77
Rodwell 3.719
Grimsley 3.592
Solomon 3.846
Lehninger 3.808
Nozaki 3.973
DTASelect 4.164
Thurlkill 3.732
EMBOSS 3.783
Sillero 3.999
Patrickios 1.888
IPC_peptide 3.846
IPC2_peptide 3.986
IPC2.peptide.svr19 3.889
Protein with the highest isoelectric point:
>tr|A0A6J0TZQ6|A0A6J0TZQ6_9SAUR myelin proteolipid protein isoform X1 OS=Pogona vitticeps OX=103695 GN=PLP1 PE=3 SV=1
MM1 pKa = 7.78 RR2 pKa = 11.84 GGMPLPGPHH11 pKa = 6.61 ISSGNGPPMRR21 pKa = 11.84 GGMSLPGPHH30 pKa = 6.74 ISSGNGPPIRR40 pKa = 11.84 GGMSLPGPRR49 pKa = 11.84 ISSGNGPPLRR59 pKa = 11.84 GGMPLPGPHH68 pKa = 6.82 ISSGHH73 pKa = 5.39 GPLMRR78 pKa = 11.84 GGMPLPGPHH87 pKa = 6.61 ISSGNGPPMRR97 pKa = 11.84 GGMSLPGPHH106 pKa = 6.74 ISSGNGPPIRR116 pKa = 11.84 GGMSLPGPRR125 pKa = 11.84 ISSGNGPPLRR135 pKa = 11.84 GGMPLPGPHH144 pKa = 6.82 ISSGHH149 pKa = 5.39 GPLMRR154 pKa = 11.84 GGMPLPGPHH163 pKa = 6.61 ISSGNGPPMRR173 pKa = 11.84 GGMSLPGPHH182 pKa = 6.74 ISSGNGPPIRR192 pKa = 11.84 GGMSLPGPRR201 pKa = 11.84 ISSGNGPPLRR211 pKa = 11.84 GGMPLPGPHH220 pKa = 6.82 ISSGHH225 pKa = 5.39 GPLMRR230 pKa = 11.84 GGMPLPGPHH239 pKa = 6.61 ISSGNGPPMRR249 pKa = 11.84 GGMSLPGPHH258 pKa = 6.91 ISSAHH263 pKa = 5.42 GPPMRR268 pKa = 11.84 GGMSLPGPHH277 pKa = 6.77 ISGNGRR283 pKa = 11.84 PIRR286 pKa = 11.84 GGMPLPGPHH295 pKa = 6.59 ISSGNGRR302 pKa = 11.84 PMRR305 pKa = 11.84 GGMPLPGPHH314 pKa = 6.76 ISSS317 pKa = 3.63
Molecular weight: 30.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.48
IPC2_protein 11.55
IPC_protein 13.159
Toseland 13.32
ProMoST 13.817
Dawson 13.32
Bjellqvist 13.32
Wikipedia 13.803
Rodwell 12.822
Grimsley 13.364
Solomon 13.817
Lehninger 13.729
Nozaki 13.32
DTASelect 13.32
Thurlkill 13.32
EMBOSS 13.817
Sillero 13.32
Patrickios 12.544
IPC_peptide 13.832
IPC2_peptide 12.822
IPC2.peptide.svr19 9.435
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
18868
12317
31185
20298321
31
35430
650.9
72.89
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.536 ± 0.012
2.175 ± 0.011
4.919 ± 0.009
7.523 ± 0.016
3.61 ± 0.01
6.018 ± 0.014
2.644 ± 0.007
4.645 ± 0.011
6.34 ± 0.014
9.521 ± 0.019
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.263 ± 0.005
3.989 ± 0.008
5.742 ± 0.017
4.889 ± 0.014
5.408 ± 0.01
8.604 ± 0.017
5.405 ± 0.009
5.895 ± 0.011
1.121 ± 0.005
2.738 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here