Mycolicibacterium brisbanense

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Mycobacteriaceae; Mycolicibacterium

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7125 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A100W3V7|A0A100W3V7_9MYCO Alanine rich transferase OS=Mycolicibacterium brisbanense OX=146020 GN=RMCB_5233 PE=4 SV=1
MM1 pKa = 7.32HH2 pKa = 7.77LAYY5 pKa = 10.38DD6 pKa = 5.7LLDD9 pKa = 4.33DD10 pKa = 4.16WDD12 pKa = 4.26ALILVDD18 pKa = 5.87ALPCQGSPGTLHH30 pKa = 6.56VFEE33 pKa = 6.69ADD35 pKa = 3.48FAGLPGPAGLDD46 pKa = 3.27AHH48 pKa = 7.38AMDD51 pKa = 5.16PAAVFAGLTALGGAPPYY68 pKa = 9.86TVVIGCEE75 pKa = 4.01VEE77 pKa = 4.58TVADD81 pKa = 5.04GIGLSDD87 pKa = 4.0SVTAAIPGAVAAIEE101 pKa = 4.1AATARR106 pKa = 11.84LANRR110 pKa = 11.84EE111 pKa = 4.09VV112 pKa = 3.16

Molecular weight: 11.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A117I5F2|A0A117I5F2_9MYCO 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1 7-dioic acid hydratase OS=Mycolicibacterium brisbanense OX=146020 GN=RMCB_2553 PE=4 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84VHH19 pKa = 5.99GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVSSRR37 pKa = 11.84RR38 pKa = 11.84SKK40 pKa = 10.72GRR42 pKa = 11.84RR43 pKa = 11.84SLTAA47 pKa = 3.9

Molecular weight: 5.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7124

1

7125

2269745

11

3672

318.6

34.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.154 ± 0.041

0.82 ± 0.008

6.265 ± 0.025

5.058 ± 0.024

3.053 ± 0.018

8.789 ± 0.029

2.28 ± 0.013

4.352 ± 0.018

2.178 ± 0.018

9.841 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.038 ± 0.012

2.265 ± 0.012

5.771 ± 0.024

3.092 ± 0.014

7.042 ± 0.03

5.385 ± 0.016

6.184 ± 0.023

8.736 ± 0.027

1.507 ± 0.012

2.19 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski