Nitrosovibrio tenuis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales; Nitrosomonadaceae; Nitrosovibrio

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2863 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H7HCH3|A0A1H7HCH3_9PROT 50S ribosomal protein L15 OS=Nitrosovibrio tenuis OX=1233 GN=rplO PE=3 SV=1
MM1 pKa = 8.09DD2 pKa = 5.3APGFTGGTQVLIDD15 pKa = 4.49RR16 pKa = 11.84EE17 pKa = 4.63AIYY20 pKa = 10.29CDD22 pKa = 3.94CGNGPRR28 pKa = 11.84VLEE31 pKa = 4.37SEE33 pKa = 4.34

Molecular weight: 3.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H7LFN9|A0A1H7LFN9_9PROT Dihydroorotate dehydrogenase (quinone) OS=Nitrosovibrio tenuis OX=1233 GN=pyrD PE=3 SV=1
MM1 pKa = 7.9EE2 pKa = 5.84DD3 pKa = 3.26RR4 pKa = 11.84ALLHH8 pKa = 7.11DD9 pKa = 6.04AICGIANALRR19 pKa = 11.84FRR21 pKa = 11.84RR22 pKa = 11.84AWQTGKK28 pKa = 10.23QYY30 pKa = 10.99RR31 pKa = 11.84KK32 pKa = 10.07LVMEE36 pKa = 5.3FVLLDD41 pKa = 3.5KK42 pKa = 11.28NGTARR47 pKa = 11.84RR48 pKa = 11.84RR49 pKa = 11.84EE50 pKa = 3.7YY51 pKa = 11.04RR52 pKa = 11.84EE53 pKa = 3.45FVRR56 pKa = 11.84ALKK59 pKa = 10.57

Molecular weight: 7.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2863

0

2863

876671

26

3461

306.2

33.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.056 ± 0.051

0.97 ± 0.015

5.165 ± 0.029

5.976 ± 0.046

3.852 ± 0.028

7.667 ± 0.053

2.428 ± 0.023

5.919 ± 0.036

4.332 ± 0.038

10.41 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.534 ± 0.023

3.541 ± 0.031

4.879 ± 0.038

3.792 ± 0.03

6.431 ± 0.043

6.071 ± 0.035

5.103 ± 0.035

6.829 ± 0.042

1.288 ± 0.017

2.756 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski