Faecalibacterium phage FP_Taranis
Average proteome isoelectric point is 6.2
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 85 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2K9V452|A0A2K9V452_9CAUD Portal protein OS=Faecalibacterium phage FP_Taranis OX=2070186 PE=4 SV=1
MM1 pKa = 6.8 NTVTVYY7 pKa = 10.81 ACPSVPMDD15 pKa = 4.76 SIEE18 pKa = 4.16 CTIEE22 pKa = 3.51 YY23 pKa = 10.64 DD24 pKa = 3.74 PAVVEE29 pKa = 5.05 AFLHH33 pKa = 6.12 PPNSGQEE40 pKa = 3.99 RR41 pKa = 11.84 ADD43 pKa = 3.94 DD44 pKa = 4.2 GSFGDD49 pKa = 4.12 EE50 pKa = 3.96 KK51 pKa = 11.53 VLL53 pKa = 4.34
Molecular weight: 5.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.788
IPC2_protein 3.91
IPC_protein 3.757
Toseland 3.592
ProMoST 3.948
Dawson 3.757
Bjellqvist 3.923
Wikipedia 3.706
Rodwell 3.605
Grimsley 3.516
Solomon 3.719
Lehninger 3.681
Nozaki 3.91
DTASelect 4.05
Thurlkill 3.668
EMBOSS 3.719
Sillero 3.884
Patrickios 0.604
IPC_peptide 3.719
IPC2_peptide 3.859
IPC2.peptide.svr19 3.804
Protein with the highest isoelectric point:
>tr|A0A2K9V475|A0A2K9V475_9CAUD Uncharacterized protein OS=Faecalibacterium phage FP_Taranis OX=2070186 PE=4 SV=1
MM1 pKa = 7.4 TSQEE5 pKa = 3.83 RR6 pKa = 11.84 HH7 pKa = 4.26 EE8 pKa = 4.47 ARR10 pKa = 11.84 YY11 pKa = 8.6 QRR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 AARR18 pKa = 11.84 RR19 pKa = 11.84 ARR21 pKa = 11.84 QEE23 pKa = 3.75 ARR25 pKa = 11.84 CAALGSLGEE34 pKa = 4.26 VFSYY38 pKa = 8.7 HH39 pKa = 6.0 TMFKK43 pKa = 10.6 YY44 pKa = 10.39 GRR46 pKa = 11.84 KK47 pKa = 8.33 CCNGIRR53 pKa = 11.84 WKK55 pKa = 10.81 QSTQNFEE62 pKa = 3.85 RR63 pKa = 11.84 HH64 pKa = 5.69 LFSHH68 pKa = 6.08 TAKK71 pKa = 9.83 QRR73 pKa = 11.84 RR74 pKa = 11.84 LILAKK79 pKa = 9.61 RR80 pKa = 11.84 WRR82 pKa = 11.84 PKK84 pKa = 10.35 KK85 pKa = 9.19 YY86 pKa = 9.97 VHH88 pKa = 5.94 FTVCEE93 pKa = 3.79 RR94 pKa = 11.84 GKK96 pKa = 10.3 IRR98 pKa = 11.84 GIDD101 pKa = 3.48 APHH104 pKa = 5.75 ITDD107 pKa = 3.64 RR108 pKa = 11.84 QIHH111 pKa = 5.31 KK112 pKa = 10.37 VISKK116 pKa = 10.21 EE117 pKa = 4.0 VLEE120 pKa = 4.27 PLYY123 pKa = 10.31 DD124 pKa = 3.52 PSMIYY129 pKa = 10.77 DD130 pKa = 3.68 NGASRR135 pKa = 11.84 IGKK138 pKa = 8.72 GLHH141 pKa = 4.59 WQIKK145 pKa = 9.66 RR146 pKa = 11.84 IKK148 pKa = 8.76 QQLARR153 pKa = 11.84 HH154 pKa = 4.47 YY155 pKa = 9.83 RR156 pKa = 11.84 KK157 pKa = 9.73 YY158 pKa = 10.87 GRR160 pKa = 11.84 AGGVLLLDD168 pKa = 4.04 LKK170 pKa = 11.15 KK171 pKa = 10.3 FFPYY175 pKa = 10.15 APHH178 pKa = 6.43 SIIYY182 pKa = 9.29 QRR184 pKa = 11.84 HH185 pKa = 3.56 QRR187 pKa = 11.84 YY188 pKa = 8.88 ILNPDD193 pKa = 3.54 FRR195 pKa = 11.84 RR196 pKa = 11.84 IADD199 pKa = 4.3 TIIDD203 pKa = 3.93 TAPGEE208 pKa = 4.25 FPGRR212 pKa = 11.84 GMPLGVEE219 pKa = 4.64 PSQQEE224 pKa = 4.02 MAAMPSAVDD233 pKa = 3.41 NWIKK237 pKa = 10.73 CQMSTHH243 pKa = 6.08 SAGHH247 pKa = 5.94 YY248 pKa = 9.21 MDD250 pKa = 6.15 DD251 pKa = 3.74 YY252 pKa = 11.48 CIILPDD258 pKa = 3.92 IEE260 pKa = 4.63 DD261 pKa = 3.67 LKK263 pKa = 11.32 KK264 pKa = 10.53 LGRR267 pKa = 11.84 AIVRR271 pKa = 11.84 QFEE274 pKa = 4.09 IRR276 pKa = 11.84 GIPVNKK282 pKa = 9.31 KK283 pKa = 7.86 KK284 pKa = 10.95 CKK286 pKa = 9.78 IIPLTKK292 pKa = 9.86 PFRR295 pKa = 11.84 WCKK298 pKa = 10.54 ARR300 pKa = 11.84 FTLTEE305 pKa = 3.75 TGKK308 pKa = 10.45 IKK310 pKa = 11.09 VNGSRR315 pKa = 11.84 DD316 pKa = 3.22 GVIRR320 pKa = 11.84 ARR322 pKa = 11.84 RR323 pKa = 11.84 KK324 pKa = 9.4 LKK326 pKa = 10.34 LFHH329 pKa = 6.95 RR330 pKa = 11.84 EE331 pKa = 3.17 WLAGKK336 pKa = 8.25 RR337 pKa = 11.84 TLQEE341 pKa = 3.39 VAQYY345 pKa = 8.11 MNCQEE350 pKa = 4.59 AYY352 pKa = 9.96 YY353 pKa = 11.05 KK354 pKa = 11.01 NFDD357 pKa = 3.28 DD358 pKa = 4.76 HH359 pKa = 6.96 GRR361 pKa = 11.84 LLRR364 pKa = 11.84 LRR366 pKa = 11.84 RR367 pKa = 11.84 LCYY370 pKa = 10.04 AIFGGRR376 pKa = 11.84 VPCSTKK382 pKa = 10.19 SSKK385 pKa = 10.49 PVMAPSLPP393 pKa = 3.58
Molecular weight: 45.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.317
IPC2_protein 9.648
IPC_protein 10.058
Toseland 10.467
ProMoST 10.101
Dawson 10.599
Bjellqvist 10.277
Wikipedia 10.774
Rodwell 10.979
Grimsley 10.657
Solomon 10.643
Lehninger 10.613
Nozaki 10.467
DTASelect 10.262
Thurlkill 10.482
EMBOSS 10.862
Sillero 10.526
Patrickios 10.643
IPC_peptide 10.657
IPC2_peptide 9.238
IPC2.peptide.svr19 8.592
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
85
0
85
16963
32
1189
199.6
22.16
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.939 ± 0.501
1.715 ± 0.138
6.29 ± 0.219
6.992 ± 0.329
3.142 ± 0.196
7.186 ± 0.249
1.916 ± 0.158
5.494 ± 0.181
5.995 ± 0.236
7.811 ± 0.267
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.847 ± 0.14
3.808 ± 0.159
3.679 ± 0.254
3.679 ± 0.241
5.241 ± 0.395
6.102 ± 0.386
6.432 ± 0.345
6.408 ± 0.215
1.462 ± 0.116
3.861 ± 0.161
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here