Alces alces faeces associated smacovirus MP78
Average proteome isoelectric point is 5.87
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z5CL51|A0A2Z5CL51_9VIRU Capsid protein OS=Alces alces faeces associated smacovirus MP78 OX=2219142 PE=4 SV=1
MM1 pKa = 6.48 TTNKK5 pKa = 10.07 AFASYY10 pKa = 10.94 HH11 pKa = 5.92 EE12 pKa = 5.38 IIDD15 pKa = 3.8 LHH17 pKa = 5.65 TEE19 pKa = 3.76 SDD21 pKa = 3.57 KK22 pKa = 11.68 VGVIGIHH29 pKa = 5.99 TPTDD33 pKa = 3.73 YY34 pKa = 10.68 TPHH37 pKa = 7.21 RR38 pKa = 11.84 MFGGYY43 pKa = 9.63 FKK45 pKa = 10.6 QFKK48 pKa = 9.05 KK49 pKa = 10.66 FKK51 pKa = 10.49 YY52 pKa = 8.83 LGCSISLVPAARR64 pKa = 11.84 LPADD68 pKa = 4.3 PSQVSYY74 pKa = 11.05 GAGEE78 pKa = 4.1 PPIDD82 pKa = 4.96 PRR84 pKa = 11.84 DD85 pKa = 3.68 LLNPIMFHH93 pKa = 6.19 GCHH96 pKa = 6.32 GNDD99 pKa = 4.09 LGNVLNRR106 pKa = 11.84 LYY108 pKa = 10.18 TLNSDD113 pKa = 3.73 GVSGGFATNIQEE125 pKa = 4.19 SDD127 pKa = 3.5 SVLKK131 pKa = 10.82 FEE133 pKa = 5.77 EE134 pKa = 4.35 ITNSYY139 pKa = 10.6 YY140 pKa = 10.76 DD141 pKa = 4.52 DD142 pKa = 3.99 NLLEE146 pKa = 4.02 NLYY149 pKa = 10.94 YY150 pKa = 10.57 KK151 pKa = 10.91 ALTDD155 pKa = 3.59 NTWRR159 pKa = 11.84 KK160 pKa = 8.79 AHH162 pKa = 5.84 PQRR165 pKa = 11.84 GFRR168 pKa = 11.84 KK169 pKa = 9.87 GGLRR173 pKa = 11.84 PLIYY177 pKa = 11.04 SMATNVQIMNPNEE190 pKa = 3.84 FSTEE194 pKa = 3.62 RR195 pKa = 11.84 LAGSNGHH202 pKa = 6.97 LSITSPINTGMPEE215 pKa = 3.79 DD216 pKa = 3.89 FAFSVSGSSGRR227 pKa = 11.84 FSRR230 pKa = 11.84 GVIDD234 pKa = 5.36 DD235 pKa = 3.93 GVKK238 pKa = 10.42 VFTPHH243 pKa = 5.83 LTGLGWLDD251 pKa = 3.42 TRR253 pKa = 11.84 SVITQGVEE261 pKa = 4.19 SDD263 pKa = 3.06 WTTLSDD269 pKa = 3.29 TDD271 pKa = 5.19 AIASAQDD278 pKa = 3.49 SIVNKK283 pKa = 10.09 EE284 pKa = 4.23 VVTTLPKK291 pKa = 10.24 IFMGVILLPPAYY303 pKa = 8.68 KK304 pKa = 9.84 TEE306 pKa = 3.6 QYY308 pKa = 10.98 YY309 pKa = 11.1 RR310 pKa = 11.84 MIINHH315 pKa = 6.47 RR316 pKa = 11.84 FGFKK320 pKa = 10.29 DD321 pKa = 3.4 FRR323 pKa = 11.84 GISFANDD330 pKa = 3.18 QLVTPAYY337 pKa = 10.77 ANFNDD342 pKa = 4.47 SDD344 pKa = 4.28 YY345 pKa = 11.02 EE346 pKa = 4.24 YY347 pKa = 11.02 NPPEE351 pKa = 4.91 DD352 pKa = 4.52 PDD354 pKa = 4.0 SGEE357 pKa = 4.04 GDD359 pKa = 3.78 SPSLSGSEE367 pKa = 3.98 YY368 pKa = 10.73 LVSDD372 pKa = 3.92 LQQYY376 pKa = 6.57 FTSYY380 pKa = 11.03 VGMGTWKK387 pKa = 10.68 LDD389 pKa = 3.27 TQYY392 pKa = 10.86 PQTVYY397 pKa = 9.2 EE398 pKa = 4.4 TLTEE402 pKa = 4.07 YY403 pKa = 11.15 DD404 pKa = 3.51 SGEE407 pKa = 4.21 STLSVSFPVQNQSDD421 pKa = 4.28 GNSTGWGYY429 pKa = 10.96 NGNWSCSAFWYY440 pKa = 10.12 LDD442 pKa = 3.04 NGFVYY447 pKa = 10.31 LGIMLCEE454 pKa = 3.61 EE455 pKa = 4.25 SLYY458 pKa = 11.13 GFYY461 pKa = 10.61 LWQNSEE467 pKa = 3.64 WTFQNWSDD475 pKa = 3.79 SLDD478 pKa = 4.34 LIGSGSITQLQADD491 pKa = 3.96 VAFAGLVNSNSSTVDD506 pKa = 3.66 DD507 pKa = 4.5 LNQYY511 pKa = 8.12 QIKK514 pKa = 10.1 SLSGNIGDD522 pKa = 4.31 VDD524 pKa = 3.71 NAA526 pKa = 4.01
Molecular weight: 58.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.141
IPC2_protein 4.406
IPC_protein 4.406
Toseland 4.215
ProMoST 4.546
Dawson 4.38
Bjellqvist 4.533
Wikipedia 4.291
Rodwell 4.24
Grimsley 4.126
Solomon 4.38
Lehninger 4.329
Nozaki 4.482
DTASelect 4.724
Thurlkill 4.24
EMBOSS 4.304
Sillero 4.533
Patrickios 2.015
IPC_peptide 4.38
IPC2_peptide 4.507
IPC2.peptide.svr19 4.435
Protein with the highest isoelectric point:
>tr|A0A2Z5CL51|A0A2Z5CL51_9VIRU Capsid protein OS=Alces alces faeces associated smacovirus MP78 OX=2219142 PE=4 SV=1
MM1 pKa = 7.38 QRR3 pKa = 11.84 TNKK6 pKa = 9.31 NRR8 pKa = 11.84 SRR10 pKa = 11.84 QVQDD14 pKa = 3.05 QSAGIISTFSCDD26 pKa = 3.19 NIYY29 pKa = 10.48 VHH31 pKa = 7.0 NAFCKK36 pKa = 8.57 TYY38 pKa = 10.21 AYY40 pKa = 8.5 HH41 pKa = 6.7 TFLSLLYY48 pKa = 9.38 EE49 pKa = 4.21 YY50 pKa = 10.51 RR51 pKa = 11.84 SNALSTLVSRR61 pKa = 11.84 MLTKK65 pKa = 9.85 ICYY68 pKa = 9.55 LRR70 pKa = 11.84 YY71 pKa = 9.24 QRR73 pKa = 11.84 CGRR76 pKa = 11.84 QEE78 pKa = 3.54 AHH80 pKa = 6.15 MVKK83 pKa = 8.42 TWMLTVPRR91 pKa = 11.84 NGDD94 pKa = 2.72 WCTTQSVCRR103 pKa = 11.84 MIQEE107 pKa = 4.07 NDD109 pKa = 3.17 VKK111 pKa = 10.72 KK112 pKa = 7.72 WTIGFEE118 pKa = 4.07 EE119 pKa = 4.82 GKK121 pKa = 10.19 NGYY124 pKa = 8.04 RR125 pKa = 11.84 HH126 pKa = 4.49 YY127 pKa = 10.66 QIRR130 pKa = 11.84 LVSSNHH136 pKa = 7.1 DD137 pKa = 3.3 FFEE140 pKa = 4.05 WCKK143 pKa = 10.72 INLPTAHH150 pKa = 6.89 IEE152 pKa = 4.08 EE153 pKa = 4.45 ATEE156 pKa = 3.66 EE157 pKa = 4.14 SGNYY161 pKa = 7.47 EE162 pKa = 3.78 RR163 pKa = 11.84 KK164 pKa = 10.02 SGNFLSSDD172 pKa = 3.29 DD173 pKa = 3.73 TAEE176 pKa = 3.67 IRR178 pKa = 11.84 QVRR181 pKa = 11.84 FGVLRR186 pKa = 11.84 EE187 pKa = 4.09 SQKK190 pKa = 11.1 KK191 pKa = 9.5 ILEE194 pKa = 4.23 EE195 pKa = 4.18 VNDD198 pKa = 3.63 QGDD201 pKa = 3.76 RR202 pKa = 11.84 EE203 pKa = 4.06 IDD205 pKa = 3.63 VYY207 pKa = 11.2 FDD209 pKa = 3.37 PRR211 pKa = 11.84 GNNGKK216 pKa = 7.55 TWLTIHH222 pKa = 6.92 LYY224 pKa = 9.71 EE225 pKa = 5.01 RR226 pKa = 11.84 GRR228 pKa = 11.84 ALVVPRR234 pKa = 11.84 ANTTAEE240 pKa = 4.09 KK241 pKa = 10.3 LSAYY245 pKa = 9.35 ICSSYY250 pKa = 11.14 NGEE253 pKa = 4.12 EE254 pKa = 4.01 YY255 pKa = 10.95 VIIDD259 pKa = 3.56 IPRR262 pKa = 11.84 SRR264 pKa = 11.84 KK265 pKa = 7.6 ITPEE269 pKa = 3.18 IYY271 pKa = 10.16 EE272 pKa = 4.69 AIEE275 pKa = 4.26 EE276 pKa = 4.33 IKK278 pKa = 10.88 DD279 pKa = 3.42 GLVFDD284 pKa = 4.33 SRR286 pKa = 11.84 YY287 pKa = 9.92 SGRR290 pKa = 11.84 SRR292 pKa = 11.84 NIRR295 pKa = 11.84 GVKK298 pKa = 10.34 LIIFTNKK305 pKa = 10.14 RR306 pKa = 11.84 LDD308 pKa = 3.57 LTKK311 pKa = 10.74 LSNDD315 pKa = 2.76 RR316 pKa = 11.84 WRR318 pKa = 11.84 LHH320 pKa = 7.11 AISTTTKK327 pKa = 10.25 RR328 pKa = 11.84 PMKK331 pKa = 9.47 TDD333 pKa = 3.55 YY334 pKa = 11.14 VPSDD338 pKa = 4.8 DD339 pKa = 4.67 DD340 pKa = 3.87 TAPDD344 pKa = 3.52 GAKK347 pKa = 10.49 AVDD350 pKa = 3.31
Molecular weight: 40.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.602
IPC2_protein 7.892
IPC_protein 7.775
Toseland 7.468
ProMoST 8.273
Dawson 8.463
Bjellqvist 8.756
Wikipedia 8.419
Rodwell 8.492
Grimsley 7.468
Solomon 8.565
Lehninger 8.565
Nozaki 8.873
DTASelect 8.521
Thurlkill 8.565
EMBOSS 8.668
Sillero 8.858
Patrickios 4.329
IPC_peptide 8.551
IPC2_peptide 7.702
IPC2.peptide.svr19 7.867
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
876
350
526
438.0
49.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.68 ± 0.103
1.37 ± 0.535
6.963 ± 0.396
5.365 ± 0.871
4.338 ± 0.698
7.42 ± 1.329
2.055 ± 0.135
5.936 ± 0.704
4.338 ± 0.97
7.648 ± 0.629
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.826 ± 0.065
6.164 ± 0.263
3.881 ± 0.765
3.767 ± 0.198
5.251 ± 2.105
9.132 ± 0.995
7.192 ± 0.138
5.479 ± 0.03
1.826 ± 0.065
5.365 ± 0.13
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here