Mycobacterium phage Predator
Average proteome isoelectric point is 6.17
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 92 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B3VM63|B3VM63_9CAUD Uncharacterized protein OS=Mycobacterium phage Predator OX=543153 GN=PREDATOR_36 PE=4 SV=1
MM1 pKa = 8.09 DD2 pKa = 5.9 AYY4 pKa = 11.2 QHH6 pKa = 6.6 FDD8 pKa = 3.01 AANAAWANVDD18 pKa = 3.52 QEE20 pKa = 4.45 QQFPEE25 pKa = 4.4 TLTTAHH31 pKa = 7.06 CLLAIAGFLKK41 pKa = 10.46 ILADD45 pKa = 3.66 NATTPP50 pKa = 3.63
Molecular weight: 5.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.921
IPC2_protein 4.291
IPC_protein 4.05
Toseland 3.872
ProMoST 4.253
Dawson 4.062
Bjellqvist 4.228
Wikipedia 4.037
Rodwell 3.897
Grimsley 3.795
Solomon 4.037
Lehninger 3.986
Nozaki 4.215
DTASelect 4.406
Thurlkill 3.961
EMBOSS 4.037
Sillero 4.19
Patrickios 1.977
IPC_peptide 4.024
IPC2_peptide 4.164
IPC2.peptide.svr19 4.075
Protein with the highest isoelectric point:
>tr|B3VM37|B3VM37_9CAUD Uncharacterized protein OS=Mycobacterium phage Predator OX=543153 GN=PREDATOR_10 PE=4 SV=1
MM1 pKa = 7.84 ASFRR5 pKa = 11.84 PKK7 pKa = 9.14 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 8.91 IFRR13 pKa = 11.84 VRR15 pKa = 11.84 TQVFAGKK22 pKa = 8.34 RR23 pKa = 11.84 TRR25 pKa = 11.84 IGLVTPGIKK34 pKa = 9.49 RR35 pKa = 11.84 ARR37 pKa = 11.84 RR38 pKa = 11.84 SRR40 pKa = 11.84 GQKK43 pKa = 9.93 AGPVQTAQPSPVLL56 pKa = 3.63
Molecular weight: 6.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
92
0
92
21371
47
2121
232.3
25.61
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.536 ± 0.477
0.973 ± 0.125
6.696 ± 0.295
6.275 ± 0.314
3.491 ± 0.21
8.736 ± 0.451
1.825 ± 0.142
5.376 ± 0.201
5.203 ± 0.272
7.131 ± 0.268
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.41 ± 0.097
4.286 ± 0.167
5.203 ± 0.238
3.762 ± 0.173
6.368 ± 0.368
5.83 ± 0.19
5.826 ± 0.204
6.551 ± 0.192
1.844 ± 0.156
2.677 ± 0.192
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here