Mycobacterium phage Predator

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Predatorvirus; Mycobacterium virus Predator

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 92 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|B3VM63|B3VM63_9CAUD Uncharacterized protein OS=Mycobacterium phage Predator OX=543153 GN=PREDATOR_36 PE=4 SV=1
MM1 pKa = 8.09DD2 pKa = 5.9AYY4 pKa = 11.2QHH6 pKa = 6.6FDD8 pKa = 3.01AANAAWANVDD18 pKa = 3.52QEE20 pKa = 4.45QQFPEE25 pKa = 4.4TLTTAHH31 pKa = 7.06CLLAIAGFLKK41 pKa = 10.46ILADD45 pKa = 3.66NATTPP50 pKa = 3.63

Molecular weight:
5.45 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|B3VM37|B3VM37_9CAUD Uncharacterized protein OS=Mycobacterium phage Predator OX=543153 GN=PREDATOR_10 PE=4 SV=1
MM1 pKa = 7.84ASFRR5 pKa = 11.84PKK7 pKa = 9.14RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 8.91IFRR13 pKa = 11.84VRR15 pKa = 11.84TQVFAGKK22 pKa = 8.34RR23 pKa = 11.84TRR25 pKa = 11.84IGLVTPGIKK34 pKa = 9.49RR35 pKa = 11.84ARR37 pKa = 11.84RR38 pKa = 11.84SRR40 pKa = 11.84GQKK43 pKa = 9.93AGPVQTAQPSPVLL56 pKa = 3.63

Molecular weight:
6.32 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

92

0

92

21371

47

2121

232.3

25.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.536 ± 0.477

0.973 ± 0.125

6.696 ± 0.295

6.275 ± 0.314

3.491 ± 0.21

8.736 ± 0.451

1.825 ± 0.142

5.376 ± 0.201

5.203 ± 0.272

7.131 ± 0.268

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.41 ± 0.097

4.286 ± 0.167

5.203 ± 0.238

3.762 ± 0.173

6.368 ± 0.368

5.83 ± 0.19

5.826 ± 0.204

6.551 ± 0.192

1.844 ± 0.156

2.677 ± 0.192

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski