Amino acid dipepetide frequency for Mycobacterium phage Predator

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.231AlaAla: 11.231 ± 1.342
0.281AlaCys: 0.281 ± 0.13
6.224AlaAsp: 6.224 ± 0.625
7.066AlaGlu: 7.066 ± 0.793
3.276AlaPhe: 3.276 ± 0.591
8.329AlaGly: 8.329 ± 1.048
1.685AlaHis: 1.685 ± 0.271
4.539AlaIle: 4.539 ± 0.529
5.803AlaLys: 5.803 ± 0.484
7.534AlaLeu: 7.534 ± 0.791
2.48AlaMet: 2.48 ± 0.278
4.258AlaAsn: 4.258 ± 0.457
4.258AlaPro: 4.258 ± 0.467
3.322AlaGln: 3.322 ± 0.517
5.849AlaArg: 5.849 ± 0.637
4.212AlaSer: 4.212 ± 0.662
4.913AlaThr: 4.913 ± 0.405
5.849AlaVal: 5.849 ± 0.495
1.685AlaTrp: 1.685 ± 0.343
2.574AlaTyr: 2.574 ± 0.388
0.0AlaXaa: 0.0 ± 0.0
Cys
1.029CysAla: 1.029 ± 0.32
0.234CysCys: 0.234 ± 0.129
0.889CysAsp: 0.889 ± 0.297
0.374CysGlu: 0.374 ± 0.167
0.374CysPhe: 0.374 ± 0.123
1.357CysGly: 1.357 ± 0.262
0.187CysHis: 0.187 ± 0.123
0.281CysIle: 0.281 ± 0.117
0.608CysLys: 0.608 ± 0.195
0.374CysLeu: 0.374 ± 0.14
0.047CysMet: 0.047 ± 0.04
0.328CysAsn: 0.328 ± 0.141
0.608CysPro: 0.608 ± 0.169
0.234CysGln: 0.234 ± 0.127
0.749CysArg: 0.749 ± 0.217
0.374CysSer: 0.374 ± 0.13
0.655CysThr: 0.655 ± 0.197
0.749CysVal: 0.749 ± 0.197
0.094CysTrp: 0.094 ± 0.068
0.187CysTyr: 0.187 ± 0.089
0.0CysXaa: 0.0 ± 0.0
Asp
5.288AspAla: 5.288 ± 0.448
0.749AspCys: 0.749 ± 0.223
4.867AspAsp: 4.867 ± 0.699
5.194AspGlu: 5.194 ± 0.669
2.901AspPhe: 2.901 ± 0.441
5.288AspGly: 5.288 ± 0.541
1.31AspHis: 1.31 ± 0.288
3.744AspIle: 3.744 ± 0.378
3.135AspLys: 3.135 ± 0.339
5.615AspLeu: 5.615 ± 0.568
1.638AspMet: 1.638 ± 0.304
2.433AspAsn: 2.433 ± 0.244
4.773AspPro: 4.773 ± 0.499
2.433AspGln: 2.433 ± 0.301
3.322AspArg: 3.322 ± 0.366
4.492AspSer: 4.492 ± 0.426
3.229AspThr: 3.229 ± 0.472
3.229AspVal: 3.229 ± 0.354
1.029AspTrp: 1.029 ± 0.224
2.246AspTyr: 2.246 ± 0.378
0.0AspXaa: 0.0 ± 0.0
Glu
5.054GluAla: 5.054 ± 0.517
0.468GluCys: 0.468 ± 0.128
5.147GluAsp: 5.147 ± 0.567
3.51GluGlu: 3.51 ± 0.536
2.714GluPhe: 2.714 ± 0.357
3.135GluGly: 3.135 ± 0.376
1.123GluHis: 1.123 ± 0.239
5.147GluIle: 5.147 ± 0.46
3.697GluLys: 3.697 ± 0.461
5.522GluLeu: 5.522 ± 0.57
1.31GluMet: 1.31 ± 0.205
2.808GluAsn: 2.808 ± 0.416
1.872GluPro: 1.872 ± 0.398
2.714GluGln: 2.714 ± 0.339
3.978GluArg: 3.978 ± 0.469
4.679GluSer: 4.679 ± 0.487
3.744GluThr: 3.744 ± 0.456
3.369GluVal: 3.369 ± 0.391
1.123GluTrp: 1.123 ± 0.195
1.638GluTyr: 1.638 ± 0.263
0.0GluXaa: 0.0 ± 0.0
Phe
3.322PheAla: 3.322 ± 0.537
0.374PheCys: 0.374 ± 0.149
2.808PheAsp: 2.808 ± 0.412
1.919PheGlu: 1.919 ± 0.3
0.796PhePhe: 0.796 ± 0.177
3.837PheGly: 3.837 ± 0.556
0.655PheHis: 0.655 ± 0.146
1.965PheIle: 1.965 ± 0.326
1.544PheLys: 1.544 ± 0.214
2.012PheLeu: 2.012 ± 0.332
0.655PheMet: 0.655 ± 0.161
1.825PheAsn: 1.825 ± 0.31
2.153PhePro: 2.153 ± 0.349
0.796PheGln: 0.796 ± 0.181
2.854PheArg: 2.854 ± 0.275
1.638PheSer: 1.638 ± 0.264
1.591PheThr: 1.591 ± 0.256
2.948PheVal: 2.948 ± 0.357
0.468PheTrp: 0.468 ± 0.145
0.749PheTyr: 0.749 ± 0.211
0.0PheXaa: 0.0 ± 0.0
Gly
6.879GlyAla: 6.879 ± 1.189
0.936GlyCys: 0.936 ± 0.226
4.679GlyAsp: 4.679 ± 0.481
5.194GlyGlu: 5.194 ± 0.433
3.088GlyPhe: 3.088 ± 0.395
8.704GlyGly: 8.704 ± 1.613
1.591GlyHis: 1.591 ± 0.363
4.539GlyIle: 4.539 ± 0.438
4.867GlyLys: 4.867 ± 0.413
6.598GlyLeu: 6.598 ± 0.85
2.761GlyMet: 2.761 ± 0.379
4.726GlyAsn: 4.726 ± 0.563
3.369GlyPro: 3.369 ± 0.392
2.761GlyGln: 2.761 ± 0.395
4.165GlyArg: 4.165 ± 0.462
6.036GlySer: 6.036 ± 0.709
5.896GlyThr: 5.896 ± 0.728
5.381GlyVal: 5.381 ± 0.537
2.106GlyTrp: 2.106 ± 0.355
3.088GlyTyr: 3.088 ± 0.518
0.0GlyXaa: 0.0 ± 0.0
His
1.919HisAla: 1.919 ± 0.385
0.655HisCys: 0.655 ± 0.156
1.217HisAsp: 1.217 ± 0.244
0.983HisGlu: 0.983 ± 0.214
0.608HisPhe: 0.608 ± 0.195
2.106HisGly: 2.106 ± 0.312
0.281HisHis: 0.281 ± 0.119
0.421HisIle: 0.421 ± 0.152
0.842HisLys: 0.842 ± 0.172
0.889HisLeu: 0.889 ± 0.184
0.562HisMet: 0.562 ± 0.127
0.468HisAsn: 0.468 ± 0.161
0.936HisPro: 0.936 ± 0.211
0.562HisGln: 0.562 ± 0.176
1.544HisArg: 1.544 ± 0.257
0.796HisSer: 0.796 ± 0.214
0.936HisThr: 0.936 ± 0.216
1.123HisVal: 1.123 ± 0.246
0.562HisTrp: 0.562 ± 0.211
0.842HisTyr: 0.842 ± 0.198
0.0HisXaa: 0.0 ± 0.0
Ile
6.083IleAla: 6.083 ± 0.607
0.562IleCys: 0.562 ± 0.182
4.024IleAsp: 4.024 ± 0.537
3.556IleGlu: 3.556 ± 0.398
2.293IlePhe: 2.293 ± 0.275
4.165IleGly: 4.165 ± 0.479
0.749IleHis: 0.749 ± 0.184
1.872IleIle: 1.872 ± 0.279
2.153IleLys: 2.153 ± 0.445
3.884IleLeu: 3.884 ± 0.4
1.263IleMet: 1.263 ± 0.197
2.246IleAsn: 2.246 ± 0.31
3.135IlePro: 3.135 ± 0.338
1.731IleGln: 1.731 ± 0.256
4.165IleArg: 4.165 ± 0.549
3.416IleSer: 3.416 ± 0.462
3.042IleThr: 3.042 ± 0.372
3.416IleVal: 3.416 ± 0.35
0.702IleTrp: 0.702 ± 0.18
1.31IleTyr: 1.31 ± 0.216
0.0IleXaa: 0.0 ± 0.0
Lys
6.177LysAla: 6.177 ± 0.713
0.14LysCys: 0.14 ± 0.088
2.62LysAsp: 2.62 ± 0.31
2.153LysGlu: 2.153 ± 0.355
1.965LysPhe: 1.965 ± 0.294
3.65LysGly: 3.65 ± 0.35
0.983LysHis: 0.983 ± 0.197
3.088LysIle: 3.088 ± 0.379
3.088LysLys: 3.088 ± 0.366
3.931LysLeu: 3.931 ± 0.406
1.404LysMet: 1.404 ± 0.225
1.825LysAsn: 1.825 ± 0.284
2.199LysPro: 2.199 ± 0.277
2.153LysGln: 2.153 ± 0.321
4.633LysArg: 4.633 ± 0.503
2.901LysSer: 2.901 ± 0.344
3.229LysThr: 3.229 ± 0.38
4.024LysVal: 4.024 ± 0.498
0.796LysTrp: 0.796 ± 0.206
1.076LysTyr: 1.076 ± 0.202
0.0LysXaa: 0.0 ± 0.0
Leu
7.3LeuAla: 7.3 ± 0.815
0.655LeuCys: 0.655 ± 0.165
4.726LeuAsp: 4.726 ± 0.454
4.726LeuGlu: 4.726 ± 0.545
2.106LeuPhe: 2.106 ± 0.358
6.551LeuGly: 6.551 ± 0.483
1.17LeuHis: 1.17 ± 0.247
3.744LeuIle: 3.744 ± 0.467
3.931LeuLys: 3.931 ± 0.385
4.258LeuLeu: 4.258 ± 0.403
0.983LeuMet: 0.983 ± 0.24
3.463LeuAsn: 3.463 ± 0.458
4.492LeuPro: 4.492 ± 0.454
2.761LeuGln: 2.761 ± 0.477
4.399LeuArg: 4.399 ± 0.359
3.931LeuSer: 3.931 ± 0.485
4.633LeuThr: 4.633 ± 0.443
4.399LeuVal: 4.399 ± 0.428
1.544LeuTrp: 1.544 ± 0.274
1.544LeuTyr: 1.544 ± 0.249
0.0LeuXaa: 0.0 ± 0.0
Met
2.667MetAla: 2.667 ± 0.411
0.187MetCys: 0.187 ± 0.107
1.31MetAsp: 1.31 ± 0.206
1.451MetGlu: 1.451 ± 0.25
0.608MetPhe: 0.608 ± 0.181
1.919MetGly: 1.919 ± 0.279
0.468MetHis: 0.468 ± 0.137
1.404MetIle: 1.404 ± 0.233
1.076MetLys: 1.076 ± 0.2
2.012MetLeu: 2.012 ± 0.233
0.608MetMet: 0.608 ± 0.207
0.842MetAsn: 0.842 ± 0.157
1.076MetPro: 1.076 ± 0.259
1.263MetGln: 1.263 ± 0.188
1.357MetArg: 1.357 ± 0.223
1.591MetSer: 1.591 ± 0.283
1.965MetThr: 1.965 ± 0.305
1.544MetVal: 1.544 ± 0.265
0.421MetTrp: 0.421 ± 0.149
0.328MetTyr: 0.328 ± 0.152
0.0MetXaa: 0.0 ± 0.0
Asn
4.165AsnAla: 4.165 ± 0.552
0.328AsnCys: 0.328 ± 0.122
2.714AsnAsp: 2.714 ± 0.348
2.34AsnGlu: 2.34 ± 0.356
1.076AsnPhe: 1.076 ± 0.278
4.726AsnGly: 4.726 ± 0.677
0.796AsnHis: 0.796 ± 0.224
2.153AsnIle: 2.153 ± 0.231
2.246AsnLys: 2.246 ± 0.276
3.088AsnLeu: 3.088 ± 0.436
0.889AsnMet: 0.889 ± 0.232
0.983AsnAsn: 0.983 ± 0.216
3.369AsnPro: 3.369 ± 0.448
1.123AsnGln: 1.123 ± 0.251
2.901AsnArg: 2.901 ± 0.392
2.34AsnSer: 2.34 ± 0.344
2.761AsnThr: 2.761 ± 0.341
2.667AsnVal: 2.667 ± 0.325
0.889AsnTrp: 0.889 ± 0.192
1.31AsnTyr: 1.31 ± 0.276
0.0AsnXaa: 0.0 ± 0.0
Pro
4.165ProAla: 4.165 ± 0.458
0.421ProCys: 0.421 ± 0.157
4.118ProAsp: 4.118 ± 0.541
3.884ProGlu: 3.884 ± 0.446
1.31ProPhe: 1.31 ± 0.225
4.633ProGly: 4.633 ± 0.525
1.076ProHis: 1.076 ± 0.268
2.246ProIle: 2.246 ± 0.366
2.995ProLys: 2.995 ± 0.395
3.135ProLeu: 3.135 ± 0.277
1.497ProMet: 1.497 ± 0.258
2.527ProAsn: 2.527 ± 0.261
2.808ProPro: 2.808 ± 0.345
2.106ProGln: 2.106 ± 0.344
2.34ProArg: 2.34 ± 0.356
3.182ProSer: 3.182 ± 0.386
3.276ProThr: 3.276 ± 0.364
4.118ProVal: 4.118 ± 0.393
0.983ProTrp: 0.983 ± 0.23
1.217ProTyr: 1.217 ± 0.229
0.0ProXaa: 0.0 ± 0.0
Gln
3.837GlnAla: 3.837 ± 0.514
0.281GlnCys: 0.281 ± 0.109
1.591GlnAsp: 1.591 ± 0.242
2.199GlnGlu: 2.199 ± 0.285
1.638GlnPhe: 1.638 ± 0.239
2.62GlnGly: 2.62 ± 0.361
0.936GlnHis: 0.936 ± 0.172
2.948GlnIle: 2.948 ± 0.301
1.919GlnLys: 1.919 ± 0.29
1.872GlnLeu: 1.872 ± 0.308
1.123GlnMet: 1.123 ± 0.199
1.497GlnAsn: 1.497 ± 0.23
1.919GlnPro: 1.919 ± 0.313
1.872GlnGln: 1.872 ± 0.416
2.34GlnArg: 2.34 ± 0.302
1.965GlnSer: 1.965 ± 0.257
2.527GlnThr: 2.527 ± 0.396
2.761GlnVal: 2.761 ± 0.306
0.749GlnTrp: 0.749 ± 0.181
1.029GlnTyr: 1.029 ± 0.227
0.0GlnXaa: 0.0 ± 0.0
Arg
5.101ArgAla: 5.101 ± 0.481
0.796ArgCys: 0.796 ± 0.218
4.024ArgAsp: 4.024 ± 0.396
4.024ArgGlu: 4.024 ± 0.49
2.153ArgPhe: 2.153 ± 0.272
4.679ArgGly: 4.679 ± 0.411
1.263ArgHis: 1.263 ± 0.221
3.978ArgIle: 3.978 ± 0.413
3.978ArgLys: 3.978 ± 0.617
4.024ArgLeu: 4.024 ± 0.401
1.919ArgMet: 1.919 ± 0.292
2.714ArgAsn: 2.714 ± 0.302
2.714ArgPro: 2.714 ± 0.424
2.574ArgGln: 2.574 ± 0.323
5.054ArgArg: 5.054 ± 0.596
3.65ArgSer: 3.65 ± 0.521
3.463ArgThr: 3.463 ± 0.459
4.679ArgVal: 4.679 ± 0.414
1.31ArgTrp: 1.31 ± 0.241
1.591ArgTyr: 1.591 ± 0.209
0.0ArgXaa: 0.0 ± 0.0
Ser
6.224SerAla: 6.224 ± 0.753
0.562SerCys: 0.562 ± 0.163
3.837SerAsp: 3.837 ± 0.413
4.679SerGlu: 4.679 ± 0.443
1.872SerPhe: 1.872 ± 0.296
6.551SerGly: 6.551 ± 0.75
0.983SerHis: 0.983 ± 0.223
2.153SerIle: 2.153 ± 0.26
2.527SerLys: 2.527 ± 0.382
4.071SerLeu: 4.071 ± 0.408
1.731SerMet: 1.731 ± 0.2
2.293SerAsn: 2.293 ± 0.363
2.667SerPro: 2.667 ± 0.337
2.012SerGln: 2.012 ± 0.362
2.667SerArg: 2.667 ± 0.364
3.931SerSer: 3.931 ± 0.605
3.135SerThr: 3.135 ± 0.343
4.071SerVal: 4.071 ± 0.502
0.889SerTrp: 0.889 ± 0.201
1.451SerTyr: 1.451 ± 0.37
0.0SerXaa: 0.0 ± 0.0
Thr
5.241ThrAla: 5.241 ± 0.463
0.842ThrCys: 0.842 ± 0.218
3.978ThrAsp: 3.978 ± 0.464
3.556ThrGlu: 3.556 ± 0.322
2.059ThrPhe: 2.059 ± 0.322
6.317ThrGly: 6.317 ± 0.628
0.936ThrHis: 0.936 ± 0.211
3.135ThrIle: 3.135 ± 0.505
2.714ThrLys: 2.714 ± 0.358
3.463ThrLeu: 3.463 ± 0.357
1.076ThrMet: 1.076 ± 0.215
2.433ThrAsn: 2.433 ± 0.335
3.51ThrPro: 3.51 ± 0.479
2.012ThrGln: 2.012 ± 0.312
3.276ThrArg: 3.276 ± 0.34
3.556ThrSer: 3.556 ± 0.409
2.995ThrThr: 2.995 ± 0.376
4.258ThrVal: 4.258 ± 0.421
1.17ThrTrp: 1.17 ± 0.218
1.731ThrTyr: 1.731 ± 0.287
0.0ThrXaa: 0.0 ± 0.0
Val
6.036ValAla: 6.036 ± 0.557
0.702ValCys: 0.702 ± 0.179
5.288ValAsp: 5.288 ± 0.54
3.51ValGlu: 3.51 ± 0.384
2.48ValPhe: 2.48 ± 0.314
5.007ValGly: 5.007 ± 0.415
0.889ValHis: 0.889 ± 0.259
3.744ValIle: 3.744 ± 0.415
3.229ValLys: 3.229 ± 0.442
5.288ValLeu: 5.288 ± 0.656
1.029ValMet: 1.029 ± 0.243
2.995ValAsn: 2.995 ± 0.404
3.744ValPro: 3.744 ± 0.346
2.948ValGln: 2.948 ± 0.332
4.305ValArg: 4.305 ± 0.558
3.51ValSer: 3.51 ± 0.366
3.51ValThr: 3.51 ± 0.446
4.352ValVal: 4.352 ± 0.401
1.263ValTrp: 1.263 ± 0.212
1.685ValTyr: 1.685 ± 0.339
0.0ValXaa: 0.0 ± 0.0
Trp
1.544TrpAla: 1.544 ± 0.268
0.234TrpCys: 0.234 ± 0.104
1.076TrpAsp: 1.076 ± 0.173
1.217TrpGlu: 1.217 ± 0.254
0.468TrpPhe: 0.468 ± 0.158
1.31TrpGly: 1.31 ± 0.263
0.515TrpHis: 0.515 ± 0.13
1.123TrpIle: 1.123 ± 0.21
0.936TrpLys: 0.936 ± 0.23
1.451TrpLeu: 1.451 ± 0.294
0.421TrpMet: 0.421 ± 0.135
0.936TrpAsn: 0.936 ± 0.268
0.842TrpPro: 0.842 ± 0.235
0.889TrpGln: 0.889 ± 0.17
1.685TrpArg: 1.685 ± 0.344
0.562TrpSer: 0.562 ± 0.175
1.17TrpThr: 1.17 ± 0.199
1.263TrpVal: 1.263 ± 0.286
0.187TrpTrp: 0.187 ± 0.09
0.608TrpTyr: 0.608 ± 0.176
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.106TyrAla: 2.106 ± 0.275
0.328TyrCys: 0.328 ± 0.131
1.825TyrAsp: 1.825 ± 0.288
1.217TyrGlu: 1.217 ± 0.241
1.123TyrPhe: 1.123 ± 0.199
2.48TyrGly: 2.48 ± 0.41
0.655TyrHis: 0.655 ± 0.23
1.544TyrIle: 1.544 ± 0.266
0.749TyrLys: 0.749 ± 0.237
2.293TyrLeu: 2.293 ± 0.323
0.655TyrMet: 0.655 ± 0.173
1.263TyrAsn: 1.263 ± 0.254
1.591TyrPro: 1.591 ± 0.339
1.357TyrGln: 1.357 ± 0.252
2.106TyrArg: 2.106 ± 0.332
1.544TyrSer: 1.544 ± 0.247
1.591TyrThr: 1.591 ± 0.317
1.31TyrVal: 1.31 ± 0.22
0.468TyrTrp: 0.468 ± 0.161
0.562TyrTyr: 0.562 ± 0.155
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 92 proteins (21371 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski