Streptococcus virus phiAbc2
Average proteome isoelectric point is 6.7
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 48 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C9WB73|C9WB73_9CAUD Capsid-tail joining protein OS=Streptococcus virus phiAbc2 OX=633135 PE=4 SV=1
MM1 pKa = 7.3 SVSKK5 pKa = 9.08 EE6 pKa = 4.26 TIMQTLNLDD15 pKa = 3.8 EE16 pKa = 4.97 TDD18 pKa = 3.78 DD19 pKa = 3.8 TALIPAYY26 pKa = 9.65 IEE28 pKa = 3.9 SARR31 pKa = 11.84 QYY33 pKa = 10.3 VVNSVGDD40 pKa = 3.54 DD41 pKa = 3.37 PKK43 pKa = 10.95 FYY45 pKa = 11.07 NLDD48 pKa = 3.41 SVRR51 pKa = 11.84 ALFDD55 pKa = 3.34 TAVIALTSSYY65 pKa = 8.66 FTYY68 pKa = 10.07 RR69 pKa = 11.84 VALTDD74 pKa = 3.37 TATYY78 pKa = 9.41 PVNLTLNSIIGQLRR92 pKa = 11.84 GLYY95 pKa = 8.66 ATYY98 pKa = 10.56 SEE100 pKa = 4.46 EE101 pKa = 5.06 RR102 pKa = 11.84 GDD104 pKa = 3.53
Molecular weight: 11.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.863
IPC2_protein 4.151
IPC_protein 4.062
Toseland 3.859
ProMoST 4.139
Dawson 4.062
Bjellqvist 4.279
Wikipedia 3.999
Rodwell 3.897
Grimsley 3.77
Solomon 4.05
Lehninger 3.999
Nozaki 4.177
DTASelect 4.406
Thurlkill 3.923
EMBOSS 4.012
Sillero 4.19
Patrickios 1.964
IPC_peptide 4.037
IPC2_peptide 4.164
IPC2.peptide.svr19 4.1
Protein with the highest isoelectric point:
>tr|C9WB93|C9WB93_9CAUD Uncharacterized protein OS=Streptococcus virus phiAbc2 OX=633135 PE=4 SV=1
MM1 pKa = 7.3 KK2 pKa = 9.82 PKK4 pKa = 10.1 RR5 pKa = 11.84 YY6 pKa = 8.98 PYY8 pKa = 10.21 SGQKK12 pKa = 9.62 KK13 pKa = 9.45 RR14 pKa = 11.84 LPKK17 pKa = 10.24 VVNAEE22 pKa = 3.91 KK23 pKa = 10.62 ALEE26 pKa = 4.01 IVINTIDD33 pKa = 3.46 SCALEE38 pKa = 4.29 RR39 pKa = 11.84 MNHH42 pKa = 6.14 KK43 pKa = 10.35 LLEE46 pKa = 4.31 SHH48 pKa = 6.6 SS49 pKa = 3.88
Molecular weight: 5.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.975
IPC2_protein 9.077
IPC_protein 9.063
Toseland 9.94
ProMoST 9.516
Dawson 10.101
Bjellqvist 9.736
Wikipedia 10.218
Rodwell 10.701
Grimsley 10.145
Solomon 10.145
Lehninger 10.131
Nozaki 9.955
DTASelect 9.706
Thurlkill 9.97
EMBOSS 10.321
Sillero 10.014
Patrickios 10.54
IPC_peptide 10.145
IPC2_peptide 8.302
IPC2.peptide.svr19 8.024
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
48
0
48
10689
42
977
222.7
25.22
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.67 ± 0.441
0.552 ± 0.146
6.483 ± 0.288
6.895 ± 0.582
4.154 ± 0.207
6.605 ± 0.514
1.488 ± 0.126
6.745 ± 0.198
8.429 ± 0.547
7.924 ± 0.407
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.498 ± 0.173
6.352 ± 0.254
2.919 ± 0.252
4.444 ± 0.346
4.275 ± 0.312
6.249 ± 0.33
6.128 ± 0.355
5.875 ± 0.303
1.413 ± 0.181
3.901 ± 0.294
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here