Amino acid dipepetide frequency for Streptococcus virus phiAbc2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.275AlaAla: 3.275 ± 0.943
0.187AlaCys: 0.187 ± 0.118
4.397AlaAsp: 4.397 ± 0.77
4.772AlaGlu: 4.772 ± 0.738
2.62AlaPhe: 2.62 ± 0.726
4.023AlaGly: 4.023 ± 0.644
0.561AlaHis: 0.561 ± 0.215
4.865AlaIle: 4.865 ± 0.762
6.082AlaLys: 6.082 ± 0.942
7.298AlaLeu: 7.298 ± 0.749
1.497AlaMet: 1.497 ± 0.317
4.678AlaAsn: 4.678 ± 0.886
1.591AlaPro: 1.591 ± 0.405
2.526AlaGln: 2.526 ± 0.637
2.433AlaArg: 2.433 ± 0.49
4.397AlaSer: 4.397 ± 0.736
3.836AlaThr: 3.836 ± 0.913
4.304AlaVal: 4.304 ± 0.883
0.936AlaTrp: 0.936 ± 0.222
2.433AlaTyr: 2.433 ± 0.482
0.0AlaXaa: 0.0 ± 0.0
Cys
0.187CysAla: 0.187 ± 0.126
0.0CysCys: 0.0 ± 0.0
0.561CysAsp: 0.561 ± 0.258
0.655CysGlu: 0.655 ± 0.311
0.374CysPhe: 0.374 ± 0.186
0.094CysGly: 0.094 ± 0.1
0.094CysHis: 0.094 ± 0.098
0.094CysIle: 0.094 ± 0.117
0.281CysLys: 0.281 ± 0.159
0.749CysLeu: 0.749 ± 0.241
0.094CysMet: 0.094 ± 0.084
0.281CysAsn: 0.281 ± 0.161
0.187CysPro: 0.187 ± 0.122
0.281CysGln: 0.281 ± 0.173
0.281CysArg: 0.281 ± 0.212
0.374CysSer: 0.374 ± 0.184
0.281CysThr: 0.281 ± 0.149
0.281CysVal: 0.281 ± 0.148
0.187CysTrp: 0.187 ± 0.122
0.187CysTyr: 0.187 ± 0.15
0.0CysXaa: 0.0 ± 0.0
Asp
3.743AspAla: 3.743 ± 0.697
0.374AspCys: 0.374 ± 0.25
3.649AspAsp: 3.649 ± 0.617
4.397AspGlu: 4.397 ± 0.771
3.649AspPhe: 3.649 ± 0.642
5.614AspGly: 5.614 ± 0.861
1.216AspHis: 1.216 ± 0.387
5.24AspIle: 5.24 ± 0.869
5.427AspLys: 5.427 ± 0.517
3.836AspLeu: 3.836 ± 0.807
2.152AspMet: 2.152 ± 0.388
4.491AspAsn: 4.491 ± 0.804
1.871AspPro: 1.871 ± 0.433
1.497AspGln: 1.497 ± 0.299
3.649AspArg: 3.649 ± 0.826
3.93AspSer: 3.93 ± 0.668
3.743AspThr: 3.743 ± 0.541
3.462AspVal: 3.462 ± 0.612
0.842AspTrp: 0.842 ± 0.27
2.058AspTyr: 2.058 ± 0.456
0.0AspXaa: 0.0 ± 0.0
Glu
4.865GluAla: 4.865 ± 0.74
0.187GluCys: 0.187 ± 0.119
4.023GluAsp: 4.023 ± 0.576
4.678GluGlu: 4.678 ± 1.113
2.433GluPhe: 2.433 ± 0.506
2.62GluGly: 2.62 ± 0.402
1.31GluHis: 1.31 ± 0.396
6.456GluIle: 6.456 ± 0.872
4.397GluLys: 4.397 ± 1.056
6.924GluLeu: 6.924 ± 1.067
2.246GluMet: 2.246 ± 0.448
3.555GluAsn: 3.555 ± 0.686
1.684GluPro: 1.684 ± 0.443
3.555GluGln: 3.555 ± 0.773
3.649GluArg: 3.649 ± 0.573
3.181GluSer: 3.181 ± 0.463
3.649GluThr: 3.649 ± 0.487
4.585GluVal: 4.585 ± 0.634
1.31GluTrp: 1.31 ± 0.33
3.649GluTyr: 3.649 ± 0.564
0.0GluXaa: 0.0 ± 0.0
Phe
2.994PheAla: 2.994 ± 0.504
0.094PheCys: 0.094 ± 0.084
3.743PheAsp: 3.743 ± 0.69
2.339PheGlu: 2.339 ± 0.419
1.497PhePhe: 1.497 ± 0.304
3.088PheGly: 3.088 ± 0.617
0.468PheHis: 0.468 ± 0.156
2.994PheIle: 2.994 ± 0.621
4.117PheLys: 4.117 ± 0.402
2.526PheLeu: 2.526 ± 0.481
0.749PheMet: 0.749 ± 0.241
2.807PheAsn: 2.807 ± 0.502
0.842PhePro: 0.842 ± 0.257
1.31PheGln: 1.31 ± 0.287
1.591PheArg: 1.591 ± 0.32
2.994PheSer: 2.994 ± 0.335
2.713PheThr: 2.713 ± 0.542
2.526PheVal: 2.526 ± 0.471
0.655PheTrp: 0.655 ± 0.263
1.497PheTyr: 1.497 ± 0.322
0.0PheXaa: 0.0 ± 0.0
Gly
2.713GlyAla: 2.713 ± 0.665
0.468GlyCys: 0.468 ± 0.198
3.743GlyAsp: 3.743 ± 0.775
4.585GlyGlu: 4.585 ± 0.63
2.62GlyPhe: 2.62 ± 0.413
4.117GlyGly: 4.117 ± 0.763
0.936GlyHis: 0.936 ± 0.345
5.614GlyIle: 5.614 ± 0.837
6.082GlyLys: 6.082 ± 0.843
6.269GlyLeu: 6.269 ± 0.577
1.684GlyMet: 1.684 ± 0.321
3.181GlyAsn: 3.181 ± 0.586
1.497GlyPro: 1.497 ± 0.551
3.462GlyGln: 3.462 ± 0.606
2.807GlyArg: 2.807 ± 0.501
4.117GlySer: 4.117 ± 0.717
4.772GlyThr: 4.772 ± 0.977
3.462GlyVal: 3.462 ± 0.653
1.123GlyTrp: 1.123 ± 0.393
2.807GlyTyr: 2.807 ± 0.489
0.0GlyXaa: 0.0 ± 0.0
His
0.374HisAla: 0.374 ± 0.199
0.094HisCys: 0.094 ± 0.094
0.936HisAsp: 0.936 ± 0.34
0.561HisGlu: 0.561 ± 0.244
0.561HisPhe: 0.561 ± 0.236
0.842HisGly: 0.842 ± 0.323
0.374HisHis: 0.374 ± 0.182
1.123HisIle: 1.123 ± 0.318
1.216HisLys: 1.216 ± 0.289
1.403HisLeu: 1.403 ± 0.339
0.655HisMet: 0.655 ± 0.242
1.31HisAsn: 1.31 ± 0.307
0.561HisPro: 0.561 ± 0.205
0.655HisGln: 0.655 ± 0.289
0.842HisArg: 0.842 ± 0.241
0.842HisSer: 0.842 ± 0.276
0.561HisThr: 0.561 ± 0.235
1.216HisVal: 1.216 ± 0.268
0.094HisTrp: 0.094 ± 0.09
0.655HisTyr: 0.655 ± 0.281
0.0HisXaa: 0.0 ± 0.0
Ile
5.146IleAla: 5.146 ± 1.142
0.468IleCys: 0.468 ± 0.202
5.052IleAsp: 5.052 ± 0.632
5.52IleGlu: 5.52 ± 0.975
2.058IlePhe: 2.058 ± 0.361
4.865IleGly: 4.865 ± 0.483
0.936IleHis: 0.936 ± 0.298
3.275IleIle: 3.275 ± 0.47
7.204IleLys: 7.204 ± 0.859
3.93IleLeu: 3.93 ± 0.809
1.965IleMet: 1.965 ± 0.487
4.959IleAsn: 4.959 ± 0.699
2.807IlePro: 2.807 ± 0.572
2.9IleGln: 2.9 ± 0.432
1.965IleArg: 1.965 ± 0.423
4.678IleSer: 4.678 ± 0.815
3.836IleThr: 3.836 ± 0.581
3.088IleVal: 3.088 ± 0.486
0.655IleTrp: 0.655 ± 0.228
2.152IleTyr: 2.152 ± 0.461
0.0IleXaa: 0.0 ± 0.0
Lys
5.707LysAla: 5.707 ± 0.661
0.374LysCys: 0.374 ± 0.213
4.959LysAsp: 4.959 ± 0.799
6.643LysGlu: 6.643 ± 0.97
3.275LysPhe: 3.275 ± 0.801
5.988LysGly: 5.988 ± 0.783
1.216LysHis: 1.216 ± 0.372
5.988LysIle: 5.988 ± 0.692
6.175LysLys: 6.175 ± 1.069
6.737LysLeu: 6.737 ± 1.025
2.339LysMet: 2.339 ± 0.463
5.427LysAsn: 5.427 ± 0.961
2.807LysPro: 2.807 ± 0.535
4.304LysGln: 4.304 ± 0.808
4.117LysArg: 4.117 ± 0.612
4.21LysSer: 4.21 ± 0.527
4.585LysThr: 4.585 ± 0.657
4.585LysVal: 4.585 ± 0.618
1.216LysTrp: 1.216 ± 0.272
3.649LysTyr: 3.649 ± 0.96
0.0LysXaa: 0.0 ± 0.0
Leu
7.111LeuAla: 7.111 ± 0.839
0.655LeuCys: 0.655 ± 0.224
4.678LeuAsp: 4.678 ± 0.667
6.924LeuGlu: 6.924 ± 0.844
2.713LeuPhe: 2.713 ± 0.379
6.456LeuGly: 6.456 ± 0.852
0.936LeuHis: 0.936 ± 0.287
4.117LeuIle: 4.117 ± 0.515
7.204LeuLys: 7.204 ± 0.884
5.052LeuLeu: 5.052 ± 0.811
2.152LeuMet: 2.152 ± 0.476
5.24LeuAsn: 5.24 ± 0.882
2.526LeuPro: 2.526 ± 0.527
2.62LeuGln: 2.62 ± 0.519
3.649LeuArg: 3.649 ± 0.705
5.146LeuSer: 5.146 ± 0.81
5.146LeuThr: 5.146 ± 0.715
4.023LeuVal: 4.023 ± 0.615
0.468LeuTrp: 0.468 ± 0.145
2.339LeuTyr: 2.339 ± 0.485
0.0LeuXaa: 0.0 ± 0.0
Met
1.871MetAla: 1.871 ± 0.448
0.0MetCys: 0.0 ± 0.0
0.749MetAsp: 0.749 ± 0.236
1.684MetGlu: 1.684 ± 0.391
1.403MetPhe: 1.403 ± 0.328
1.123MetGly: 1.123 ± 0.301
0.187MetHis: 0.187 ± 0.153
1.778MetIle: 1.778 ± 0.3
3.181MetLys: 3.181 ± 0.569
2.152MetLeu: 2.152 ± 0.374
0.749MetMet: 0.749 ± 0.285
1.497MetAsn: 1.497 ± 0.285
0.749MetPro: 0.749 ± 0.248
1.123MetGln: 1.123 ± 0.332
0.842MetArg: 0.842 ± 0.192
1.778MetSer: 1.778 ± 0.504
1.684MetThr: 1.684 ± 0.425
1.403MetVal: 1.403 ± 0.352
0.187MetTrp: 0.187 ± 0.127
0.842MetTyr: 0.842 ± 0.325
0.0MetXaa: 0.0 ± 0.0
Asn
5.146AsnAla: 5.146 ± 0.778
0.281AsnCys: 0.281 ± 0.169
3.743AsnAsp: 3.743 ± 0.603
3.462AsnGlu: 3.462 ± 0.699
3.275AsnPhe: 3.275 ± 0.561
5.894AsnGly: 5.894 ± 1.207
1.497AsnHis: 1.497 ± 0.431
4.117AsnIle: 4.117 ± 0.567
4.491AsnLys: 4.491 ± 0.503
4.865AsnLeu: 4.865 ± 0.497
0.749AsnMet: 0.749 ± 0.243
3.275AsnAsn: 3.275 ± 0.626
2.807AsnPro: 2.807 ± 0.559
2.9AsnGln: 2.9 ± 0.6
2.152AsnArg: 2.152 ± 0.418
3.836AsnSer: 3.836 ± 0.757
3.743AsnThr: 3.743 ± 0.645
3.743AsnVal: 3.743 ± 0.577
1.123AsnTrp: 1.123 ± 0.287
2.433AsnTyr: 2.433 ± 0.448
0.0AsnXaa: 0.0 ± 0.0
Pro
1.965ProAla: 1.965 ± 0.38
0.0ProCys: 0.0 ± 0.0
1.216ProAsp: 1.216 ± 0.362
1.965ProGlu: 1.965 ± 0.432
0.936ProPhe: 0.936 ± 0.289
0.936ProGly: 0.936 ± 0.375
0.468ProHis: 0.468 ± 0.195
1.403ProIle: 1.403 ± 0.314
3.93ProLys: 3.93 ± 0.709
2.433ProLeu: 2.433 ± 0.416
0.094ProMet: 0.094 ± 0.1
2.713ProAsn: 2.713 ± 0.592
0.281ProPro: 0.281 ± 0.189
2.058ProGln: 2.058 ± 0.435
1.216ProArg: 1.216 ± 0.399
2.058ProSer: 2.058 ± 0.452
1.965ProThr: 1.965 ± 0.386
1.684ProVal: 1.684 ± 0.518
0.468ProTrp: 0.468 ± 0.223
1.403ProTyr: 1.403 ± 0.409
0.0ProXaa: 0.0 ± 0.0
Gln
3.462GlnAla: 3.462 ± 0.912
0.187GlnCys: 0.187 ± 0.119
2.713GlnAsp: 2.713 ± 0.398
3.181GlnGlu: 3.181 ± 0.679
1.684GlnPhe: 1.684 ± 0.298
2.994GlnGly: 2.994 ± 0.697
0.468GlnHis: 0.468 ± 0.205
3.088GlnIle: 3.088 ± 0.615
3.181GlnLys: 3.181 ± 0.543
3.743GlnLeu: 3.743 ± 0.577
1.684GlnMet: 1.684 ± 0.437
2.713GlnAsn: 2.713 ± 0.462
0.842GlnPro: 0.842 ± 0.234
2.9GlnGln: 2.9 ± 0.731
1.684GlnArg: 1.684 ± 0.379
2.526GlnSer: 2.526 ± 0.53
2.62GlnThr: 2.62 ± 0.509
2.058GlnVal: 2.058 ± 0.608
0.749GlnTrp: 0.749 ± 0.194
1.965GlnTyr: 1.965 ± 0.437
0.0GlnXaa: 0.0 ± 0.0
Arg
2.526ArgAla: 2.526 ± 0.322
0.094ArgCys: 0.094 ± 0.1
3.555ArgAsp: 3.555 ± 1.006
2.526ArgGlu: 2.526 ± 0.591
1.965ArgPhe: 1.965 ± 0.388
2.152ArgGly: 2.152 ± 0.45
0.655ArgHis: 0.655 ± 0.222
2.713ArgIle: 2.713 ± 0.506
3.368ArgLys: 3.368 ± 0.725
3.368ArgLeu: 3.368 ± 0.816
1.216ArgMet: 1.216 ± 0.368
2.339ArgAsn: 2.339 ± 0.39
1.123ArgPro: 1.123 ± 0.328
2.807ArgGln: 2.807 ± 0.5
1.216ArgArg: 1.216 ± 0.338
1.591ArgSer: 1.591 ± 0.44
3.088ArgThr: 3.088 ± 0.704
2.713ArgVal: 2.713 ± 0.451
1.123ArgTrp: 1.123 ± 0.319
2.62ArgTyr: 2.62 ± 0.528
0.0ArgXaa: 0.0 ± 0.0
Ser
3.743SerAla: 3.743 ± 0.459
0.655SerCys: 0.655 ± 0.263
4.397SerAsp: 4.397 ± 0.565
3.555SerGlu: 3.555 ± 0.449
3.088SerPhe: 3.088 ± 0.653
4.772SerGly: 4.772 ± 0.829
0.749SerHis: 0.749 ± 0.259
4.117SerIle: 4.117 ± 0.533
4.21SerLys: 4.21 ± 0.659
4.023SerLeu: 4.023 ± 0.518
1.778SerMet: 1.778 ± 0.386
4.772SerAsn: 4.772 ± 0.69
1.871SerPro: 1.871 ± 0.442
2.994SerGln: 2.994 ± 0.515
3.088SerArg: 3.088 ± 0.691
4.117SerSer: 4.117 ± 0.615
3.743SerThr: 3.743 ± 0.612
4.678SerVal: 4.678 ± 0.6
0.468SerTrp: 0.468 ± 0.238
1.684SerTyr: 1.684 ± 0.418
0.0SerXaa: 0.0 ± 0.0
Thr
4.772ThrAla: 4.772 ± 0.714
0.374ThrCys: 0.374 ± 0.179
3.743ThrAsp: 3.743 ± 0.619
3.836ThrGlu: 3.836 ± 0.746
2.713ThrPhe: 2.713 ± 0.62
4.21ThrGly: 4.21 ± 0.591
0.749ThrHis: 0.749 ± 0.222
4.585ThrIle: 4.585 ± 1.091
4.772ThrLys: 4.772 ± 0.617
5.894ThrLeu: 5.894 ± 0.745
0.842ThrMet: 0.842 ± 0.3
3.93ThrAsn: 3.93 ± 0.565
1.591ThrPro: 1.591 ± 0.4
2.339ThrGln: 2.339 ± 0.597
2.433ThrArg: 2.433 ± 0.456
3.462ThrSer: 3.462 ± 0.608
3.368ThrThr: 3.368 ± 0.654
3.649ThrVal: 3.649 ± 0.66
1.029ThrTrp: 1.029 ± 0.274
2.994ThrTyr: 2.994 ± 0.551
0.0ThrXaa: 0.0 ± 0.0
Val
3.93ValAla: 3.93 ± 0.648
0.374ValCys: 0.374 ± 0.193
5.052ValAsp: 5.052 ± 0.555
4.397ValGlu: 4.397 ± 0.654
2.246ValPhe: 2.246 ± 0.542
3.93ValGly: 3.93 ± 0.564
0.655ValHis: 0.655 ± 0.236
2.994ValIle: 2.994 ± 0.475
4.397ValLys: 4.397 ± 0.711
3.275ValLeu: 3.275 ± 0.576
1.123ValMet: 1.123 ± 0.309
3.462ValAsn: 3.462 ± 0.703
2.246ValPro: 2.246 ± 0.497
1.497ValGln: 1.497 ± 0.432
2.526ValArg: 2.526 ± 0.514
5.146ValSer: 5.146 ± 0.736
5.333ValThr: 5.333 ± 0.75
3.462ValVal: 3.462 ± 0.618
1.123ValTrp: 1.123 ± 0.363
1.591ValTyr: 1.591 ± 0.384
0.0ValXaa: 0.0 ± 0.0
Trp
0.561TrpAla: 0.561 ± 0.217
0.0TrpCys: 0.0 ± 0.0
1.029TrpAsp: 1.029 ± 0.441
0.936TrpGlu: 0.936 ± 0.223
0.749TrpPhe: 0.749 ± 0.224
0.561TrpGly: 0.561 ± 0.289
0.374TrpHis: 0.374 ± 0.178
0.561TrpIle: 0.561 ± 0.218
1.029TrpLys: 1.029 ± 0.328
1.403TrpLeu: 1.403 ± 0.295
0.094TrpMet: 0.094 ± 0.09
0.936TrpAsn: 0.936 ± 0.367
0.094TrpPro: 0.094 ± 0.094
0.561TrpGln: 0.561 ± 0.209
0.655TrpArg: 0.655 ± 0.228
1.497TrpSer: 1.497 ± 0.578
0.842TrpThr: 0.842 ± 0.223
1.778TrpVal: 1.778 ± 0.391
0.281TrpTrp: 0.281 ± 0.192
0.187TrpTyr: 0.187 ± 0.122
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.62TyrAla: 2.62 ± 0.491
0.655TyrCys: 0.655 ± 0.294
2.9TyrAsp: 2.9 ± 0.537
2.339TyrGlu: 2.339 ± 0.408
1.684TyrPhe: 1.684 ± 0.41
1.778TyrGly: 1.778 ± 0.39
1.029TyrHis: 1.029 ± 0.456
2.339TyrIle: 2.339 ± 0.445
3.555TyrLys: 3.555 ± 0.501
3.368TyrLeu: 3.368 ± 0.449
1.029TyrMet: 1.029 ± 0.313
1.965TyrAsn: 1.965 ± 0.449
1.216TyrPro: 1.216 ± 0.329
2.152TyrGln: 2.152 ± 0.455
1.965TyrArg: 1.965 ± 0.327
2.62TyrSer: 2.62 ± 0.575
1.778TyrThr: 1.778 ± 0.395
2.058TyrVal: 2.058 ± 0.349
0.094TyrTrp: 0.094 ± 0.086
1.871TyrTyr: 1.871 ± 0.653
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 48 proteins (10689 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski