Canary circovirus (CaCV)

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; Circovirus

Average proteome isoelectric point is 8.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q912W1|REP_CACV Replication-associated protein OS=Canary circovirus OX=142661 GN=Rep PE=3 SV=2
MM1 pKa = 7.41APVRR5 pKa = 11.84AAAAKK10 pKa = 9.69RR11 pKa = 11.84WCFTLNNYY19 pKa = 7.09TAEE22 pKa = 4.14EE23 pKa = 4.05EE24 pKa = 4.38AKK26 pKa = 10.63VRR28 pKa = 11.84ALLPGEE34 pKa = 3.98FHH36 pKa = 7.34FAICGKK42 pKa = 9.91EE43 pKa = 3.8RR44 pKa = 11.84GEE46 pKa = 4.02QGTPHH51 pKa = 6.37LQGFLHH57 pKa = 6.55FKK59 pKa = 10.21KK60 pKa = 10.25KK61 pKa = 10.04QRR63 pKa = 11.84LSALKK68 pKa = 10.18KK69 pKa = 10.2LLARR73 pKa = 11.84AHH75 pKa = 5.63WEE77 pKa = 3.72KK78 pKa = 11.16ARR80 pKa = 11.84GSDD83 pKa = 3.44HH84 pKa = 7.57DD85 pKa = 4.0NEE87 pKa = 4.75EE88 pKa = 4.41YY89 pKa = 10.37CSKK92 pKa = 10.78EE93 pKa = 3.44NDD95 pKa = 3.48VILTIGEE102 pKa = 4.15PVQGNRR108 pKa = 11.84SDD110 pKa = 3.63LAGAVAAVKK119 pKa = 10.28AGRR122 pKa = 11.84RR123 pKa = 11.84MVDD126 pKa = 2.83IARR129 pKa = 11.84EE130 pKa = 3.71FSEE133 pKa = 5.32IYY135 pKa = 10.01VKK137 pKa = 10.67YY138 pKa = 10.78GRR140 pKa = 11.84GLRR143 pKa = 11.84DD144 pKa = 3.42LALMIGQKK152 pKa = 10.19PRR154 pKa = 11.84DD155 pKa = 3.83FKK157 pKa = 11.11TEE159 pKa = 3.91VVVITGPSGVGKK171 pKa = 10.46SRR173 pKa = 11.84LASEE177 pKa = 4.37MEE179 pKa = 3.85GSKK182 pKa = 10.15FYY184 pKa = 11.27KK185 pKa = 9.95MKK187 pKa = 10.67GDD189 pKa = 2.93WWDD192 pKa = 3.88GYY194 pKa = 10.31SNEE197 pKa = 5.58DD198 pKa = 3.36IVIMDD203 pKa = 5.24DD204 pKa = 4.19FYY206 pKa = 11.81GWLPFCEE213 pKa = 4.6MLRR216 pKa = 11.84LMDD219 pKa = 5.04RR220 pKa = 11.84YY221 pKa = 8.67PHH223 pKa = 6.18KK224 pKa = 11.24VPVKK228 pKa = 8.85GSYY231 pKa = 10.43VEE233 pKa = 4.25FTSKK237 pKa = 10.73KK238 pKa = 10.06IVITSNTHH246 pKa = 5.37PEE248 pKa = 3.48SWYY251 pKa = 11.1CPDD254 pKa = 3.35KK255 pKa = 11.11CYY257 pKa = 10.95LPALFRR263 pKa = 11.84RR264 pKa = 11.84INKK267 pKa = 7.82WMYY270 pKa = 8.45WDD272 pKa = 3.61GLRR275 pKa = 11.84FEE277 pKa = 5.45DD278 pKa = 5.39VPDD281 pKa = 3.61AMKK284 pKa = 10.45KK285 pKa = 10.12HH286 pKa = 6.56PINYY290 pKa = 9.65

Molecular weight: 33.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q912W1|REP_CACV Replication-associated protein OS=Canary circovirus OX=142661 GN=Rep PE=3 SV=2
MM1 pKa = 7.9WLTFNQVARR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84PLAPRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84RR21 pKa = 11.84WRR23 pKa = 11.84RR24 pKa = 11.84RR25 pKa = 11.84YY26 pKa = 5.77WXRR29 pKa = 11.84RR30 pKa = 11.84RR31 pKa = 11.84RR32 pKa = 11.84IPANRR37 pKa = 11.84RR38 pKa = 11.84GHH40 pKa = 5.12RR41 pKa = 11.84TNRR44 pKa = 11.84VYY46 pKa = 10.68RR47 pKa = 11.84FRR49 pKa = 11.84FVRR52 pKa = 11.84EE53 pKa = 4.23FGQVLQKK60 pKa = 10.35GTGGSQLSFGTDD72 pKa = 4.44GINIILDD79 pKa = 3.94DD80 pKa = 4.27FLDD83 pKa = 3.53WGTINWRR90 pKa = 11.84LPFEE94 pKa = 4.93DD95 pKa = 3.68YY96 pKa = 10.59RR97 pKa = 11.84IRR99 pKa = 11.84LAKK102 pKa = 10.6VEE104 pKa = 4.0MRR106 pKa = 11.84PLNEE110 pKa = 4.37SWEE113 pKa = 4.05EE114 pKa = 3.78WKK116 pKa = 11.1GFGHH120 pKa = 6.6NVPIQDD126 pKa = 3.26NHH128 pKa = 8.53LEE130 pKa = 4.17DD131 pKa = 4.3FFKK134 pKa = 10.08KK135 pKa = 8.93TRR137 pKa = 11.84LDD139 pKa = 3.93ADD141 pKa = 4.22PLANWDD147 pKa = 4.03GARR150 pKa = 11.84KK151 pKa = 8.58WDD153 pKa = 3.55LRR155 pKa = 11.84KK156 pKa = 10.27GFKK159 pKa = 10.19RR160 pKa = 11.84LFKK163 pKa = 10.23PRR165 pKa = 11.84PQLSVTDD172 pKa = 3.61TDD174 pKa = 3.95AANVTAALWLNNPKK188 pKa = 10.36SLWIPIMKK196 pKa = 10.3KK197 pKa = 10.01SDD199 pKa = 3.38QNLPSSGTRR208 pKa = 11.84VKK210 pKa = 10.58HH211 pKa = 5.59YY212 pKa = 10.61GLAFSWPEE220 pKa = 3.61PTPNQMDD227 pKa = 3.84YY228 pKa = 10.45QVKK231 pKa = 7.89VTIYY235 pKa = 11.0CEE237 pKa = 3.9FRR239 pKa = 11.84QMNLTHH245 pKa = 7.38LATPKK250 pKa = 10.64

Molecular weight: 29.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

540

250

290

270.0

31.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.667 ± 0.965

1.296 ± 0.59

5.926 ± 0.049

5.556 ± 1.287

5.0 ± 0.395

6.667 ± 0.702

2.407 ± 0.268

4.259 ± 0.171

7.593 ± 1.048

7.963 ± 0.551

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.778 ± 0.512

4.444 ± 1.024

5.556 ± 0.556

2.778 ± 0.804

9.815 ± 2.227

4.259 ± 0.434

4.259 ± 0.882

5.37 ± 0.639

3.704 ± 0.721

3.519 ± 0.736
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski