Bordetella sp. N

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Alcaligenaceae; Bordetella; unclassified Bordetella

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5821 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0S1Y2K9|A0A0S1Y2K9_9BORD Type IV secretion protein Rhs OS=Bordetella sp. N OX=1746199 GN=ASB57_15985 PE=3 SV=1
MM1 pKa = 6.91NTDD4 pKa = 3.08TSSIDD9 pKa = 3.42TPTAQPLRR17 pKa = 11.84TWQCILCGFVYY28 pKa = 10.64DD29 pKa = 4.47EE30 pKa = 4.26ATGLPEE36 pKa = 4.01EE37 pKa = 5.3GIPAGTRR44 pKa = 11.84WEE46 pKa = 4.5DD47 pKa = 4.3VPDD50 pKa = 3.26TWICPDD56 pKa = 3.92CSATKK61 pKa = 10.66ADD63 pKa = 4.25FEE65 pKa = 4.81MIAII69 pKa = 4.76

Molecular weight: 7.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0S1Y5V1|A0A0S1Y5V1_9BORD Threonine aldolase OS=Bordetella sp. N OX=1746199 GN=ASB57_23095 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVTRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.0RR14 pKa = 11.84THH16 pKa = 5.76GFRR19 pKa = 11.84VRR21 pKa = 11.84MKK23 pKa = 9.39TRR25 pKa = 11.84GGRR28 pKa = 11.84AVLNARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.65GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5821

0

5821

2020596

43

4907

347.1

37.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.949 ± 0.047

0.815 ± 0.01

5.609 ± 0.025

4.777 ± 0.036

3.304 ± 0.02

8.649 ± 0.052

2.143 ± 0.015

4.525 ± 0.021

2.985 ± 0.028

10.598 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.4 ± 0.016

2.719 ± 0.025

5.319 ± 0.026

3.853 ± 0.02

6.897 ± 0.041

5.4 ± 0.027

5.548 ± 0.049

7.713 ± 0.025

1.351 ± 0.011

2.448 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski