Amino acid dipepetide frequency for Azoarcus sp. CIB

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.578AlaAla: 17.578 ± 0.169
1.433AlaCys: 1.433 ± 0.033
6.903AlaAsp: 6.903 ± 0.086
7.575AlaGlu: 7.575 ± 0.086
4.148AlaPhe: 4.148 ± 0.056
11.065AlaGly: 11.065 ± 0.12
2.638AlaHis: 2.638 ± 0.04
5.72AlaIle: 5.72 ± 0.059
3.603AlaLys: 3.603 ± 0.057
14.097AlaLeu: 14.097 ± 0.12
3.255AlaMet: 3.255 ± 0.053
2.976AlaAsn: 2.976 ± 0.042
5.867AlaPro: 5.867 ± 0.085
4.323AlaGln: 4.323 ± 0.061
9.504AlaArg: 9.504 ± 0.102
6.054AlaSer: 6.054 ± 0.065
5.985AlaThr: 5.985 ± 0.071
8.914AlaVal: 8.914 ± 0.09
1.749AlaTrp: 1.749 ± 0.036
2.419AlaTyr: 2.419 ± 0.04
0.0AlaXaa: 0.0 ± 0.0
Cys
1.236CysAla: 1.236 ± 0.03
0.145CysCys: 0.145 ± 0.009
0.648CysAsp: 0.648 ± 0.023
0.597CysGlu: 0.597 ± 0.021
0.34CysPhe: 0.34 ± 0.013
1.104CysGly: 1.104 ± 0.028
0.387CysHis: 0.387 ± 0.027
0.435CysIle: 0.435 ± 0.015
0.257CysLys: 0.257 ± 0.012
0.885CysLeu: 0.885 ± 0.027
0.202CysMet: 0.202 ± 0.012
0.268CysAsn: 0.268 ± 0.013
0.602CysPro: 0.602 ± 0.022
0.249CysGln: 0.249 ± 0.012
0.764CysArg: 0.764 ± 0.022
0.55CysSer: 0.55 ± 0.019
0.53CysThr: 0.53 ± 0.021
0.765CysVal: 0.765 ± 0.023
0.127CysTrp: 0.127 ± 0.01
0.243CysTyr: 0.243 ± 0.012
0.0CysXaa: 0.0 ± 0.0
Asp
7.412AspAla: 7.412 ± 0.086
0.612AspCys: 0.612 ± 0.019
3.237AspAsp: 3.237 ± 0.052
3.748AspGlu: 3.748 ± 0.052
2.277AspPhe: 2.277 ± 0.036
5.128AspGly: 5.128 ± 0.082
1.155AspHis: 1.155 ± 0.029
2.654AspIle: 2.654 ± 0.044
1.728AspLys: 1.728 ± 0.035
5.555AspLeu: 5.555 ± 0.067
1.165AspMet: 1.165 ± 0.026
1.275AspAsn: 1.275 ± 0.032
3.119AspPro: 3.119 ± 0.044
1.563AspGln: 1.563 ± 0.038
3.934AspArg: 3.934 ± 0.056
2.352AspSer: 2.352 ± 0.043
2.706AspThr: 2.706 ± 0.055
4.108AspVal: 4.108 ± 0.048
1.008AspTrp: 1.008 ± 0.027
1.548AspTyr: 1.548 ± 0.035
0.0AspXaa: 0.0 ± 0.0
Glu
7.269GluAla: 7.269 ± 0.081
0.505GluCys: 0.505 ± 0.017
2.692GluAsp: 2.692 ± 0.045
3.023GluGlu: 3.023 ± 0.057
2.0GluPhe: 2.0 ± 0.038
4.122GluGly: 4.122 ± 0.048
1.543GluHis: 1.543 ± 0.029
3.263GluIle: 3.263 ± 0.055
2.044GluLys: 2.044 ± 0.042
6.607GluLeu: 6.607 ± 0.081
1.472GluMet: 1.472 ± 0.031
1.518GluAsn: 1.518 ± 0.031
2.547GluPro: 2.547 ± 0.043
2.403GluGln: 2.403 ± 0.046
5.638GluArg: 5.638 ± 0.07
2.88GluSer: 2.88 ± 0.046
2.909GluThr: 2.909 ± 0.045
4.34GluVal: 4.34 ± 0.06
0.81GluTrp: 0.81 ± 0.024
1.199GluTyr: 1.199 ± 0.031
0.0GluXaa: 0.0 ± 0.0
Phe
4.591PheAla: 4.591 ± 0.057
0.417PheCys: 0.417 ± 0.02
2.54PheAsp: 2.54 ± 0.043
2.189PheGlu: 2.189 ± 0.039
1.406PhePhe: 1.406 ± 0.034
3.555PheGly: 3.555 ± 0.051
0.802PheHis: 0.802 ± 0.024
1.517PheIle: 1.517 ± 0.034
1.004PheLys: 1.004 ± 0.023
3.211PheLeu: 3.211 ± 0.049
0.776PheMet: 0.776 ± 0.022
1.032PheAsn: 1.032 ± 0.028
1.667PhePro: 1.667 ± 0.04
0.912PheGln: 0.912 ± 0.026
2.435PheArg: 2.435 ± 0.04
2.071PheSer: 2.071 ± 0.044
1.765PheThr: 1.765 ± 0.05
2.972PheVal: 2.972 ± 0.049
0.474PheTrp: 0.474 ± 0.02
0.876PheTyr: 0.876 ± 0.022
0.0PheXaa: 0.0 ± 0.0
Gly
9.286GlyAla: 9.286 ± 0.089
1.021GlyCys: 1.021 ± 0.026
4.433GlyAsp: 4.433 ± 0.059
5.241GlyGlu: 5.241 ± 0.068
3.4GlyPhe: 3.4 ± 0.058
7.136GlyGly: 7.136 ± 0.095
1.929GlyHis: 1.929 ± 0.038
4.266GlyIle: 4.266 ± 0.052
3.31GlyLys: 3.31 ± 0.052
8.705GlyLeu: 8.705 ± 0.092
2.365GlyMet: 2.365 ± 0.039
2.298GlyAsn: 2.298 ± 0.055
2.922GlyPro: 2.922 ± 0.044
2.73GlyGln: 2.73 ± 0.043
6.36GlyArg: 6.36 ± 0.08
4.445GlySer: 4.445 ± 0.06
4.344GlyThr: 4.344 ± 0.078
6.347GlyVal: 6.347 ± 0.074
1.398GlyTrp: 1.398 ± 0.033
2.252GlyTyr: 2.252 ± 0.034
0.0GlyXaa: 0.0 ± 0.0
His
2.832HisAla: 2.832 ± 0.048
0.294HisCys: 0.294 ± 0.012
1.383HisAsp: 1.383 ± 0.031
1.206HisGlu: 1.206 ± 0.029
0.917HisPhe: 0.917 ± 0.026
2.157HisGly: 2.157 ± 0.041
0.62HisHis: 0.62 ± 0.025
0.933HisIle: 0.933 ± 0.023
0.567HisLys: 0.567 ± 0.021
2.274HisLeu: 2.274 ± 0.041
0.474HisMet: 0.474 ± 0.018
0.542HisAsn: 0.542 ± 0.019
1.51HisPro: 1.51 ± 0.028
0.6HisGln: 0.6 ± 0.019
1.644HisArg: 1.644 ± 0.031
1.01HisSer: 1.01 ± 0.028
1.013HisThr: 1.013 ± 0.026
1.598HisVal: 1.598 ± 0.035
0.366HisTrp: 0.366 ± 0.016
0.658HisTyr: 0.658 ± 0.02
0.0HisXaa: 0.0 ± 0.0
Ile
6.619IleAla: 6.619 ± 0.064
0.46IleCys: 0.46 ± 0.017
3.338IleAsp: 3.338 ± 0.05
3.357IleGlu: 3.357 ± 0.045
1.418IlePhe: 1.418 ± 0.034
4.415IleGly: 4.415 ± 0.061
0.98IleHis: 0.98 ± 0.027
1.644IleIle: 1.644 ± 0.04
1.301IleLys: 1.301 ± 0.029
3.879IleLeu: 3.879 ± 0.06
0.802IleMet: 0.802 ± 0.025
1.392IleAsn: 1.392 ± 0.033
2.312IlePro: 2.312 ± 0.038
1.162IleGln: 1.162 ± 0.033
3.224IleArg: 3.224 ± 0.045
2.368IleSer: 2.368 ± 0.042
2.36IleThr: 2.36 ± 0.041
3.936IleVal: 3.936 ± 0.056
0.472IleTrp: 0.472 ± 0.018
1.024IleTyr: 1.024 ± 0.025
0.0IleXaa: 0.0 ± 0.0
Lys
3.554LysAla: 3.554 ± 0.059
0.231LysCys: 0.231 ± 0.013
1.625LysAsp: 1.625 ± 0.041
1.59LysGlu: 1.59 ± 0.036
0.9LysPhe: 0.9 ± 0.024
2.356LysGly: 2.356 ± 0.038
0.655LysHis: 0.655 ± 0.022
1.546LysIle: 1.546 ± 0.037
1.174LysLys: 1.174 ± 0.038
3.45LysLeu: 3.45 ± 0.052
0.776LysMet: 0.776 ± 0.024
0.899LysAsn: 0.899 ± 0.023
1.86LysPro: 1.86 ± 0.036
1.083LysGln: 1.083 ± 0.027
2.259LysArg: 2.259 ± 0.036
1.698LysSer: 1.698 ± 0.031
1.723LysThr: 1.723 ± 0.038
2.507LysVal: 2.507 ± 0.042
0.356LysTrp: 0.356 ± 0.015
0.704LysTyr: 0.704 ± 0.025
0.0LysXaa: 0.0 ± 0.0
Leu
15.072LeuAla: 15.072 ± 0.136
1.047LeuCys: 1.047 ± 0.026
6.368LeuAsp: 6.368 ± 0.073
5.727LeuGlu: 5.727 ± 0.07
3.547LeuPhe: 3.547 ± 0.051
8.499LeuGly: 8.499 ± 0.08
2.217LeuHis: 2.217 ± 0.04
4.651LeuIle: 4.651 ± 0.062
3.408LeuLys: 3.408 ± 0.054
10.717LeuLeu: 10.717 ± 0.103
2.363LeuMet: 2.363 ± 0.047
2.611LeuAsn: 2.611 ± 0.047
6.073LeuPro: 6.073 ± 0.074
3.328LeuGln: 3.328 ± 0.044
8.156LeuArg: 8.156 ± 0.094
5.552LeuSer: 5.552 ± 0.06
5.255LeuThr: 5.255 ± 0.061
7.826LeuVal: 7.826 ± 0.089
1.243LeuTrp: 1.243 ± 0.035
2.168LeuTyr: 2.168 ± 0.038
0.0LeuXaa: 0.0 ± 0.0
Met
2.693MetAla: 2.693 ± 0.041
0.198MetCys: 0.198 ± 0.01
1.149MetAsp: 1.149 ± 0.028
1.153MetGlu: 1.153 ± 0.032
0.716MetPhe: 0.716 ± 0.022
1.716MetGly: 1.716 ± 0.035
0.555MetHis: 0.555 ± 0.019
1.082MetIle: 1.082 ± 0.03
1.052MetLys: 1.052 ± 0.026
2.62MetLeu: 2.62 ± 0.046
0.653MetMet: 0.653 ± 0.021
0.843MetAsn: 0.843 ± 0.02
1.388MetPro: 1.388 ± 0.031
0.93MetGln: 0.93 ± 0.022
1.807MetArg: 1.807 ± 0.035
1.605MetSer: 1.605 ± 0.034
1.493MetThr: 1.493 ± 0.03
1.586MetVal: 1.586 ± 0.037
0.227MetTrp: 0.227 ± 0.011
0.386MetTyr: 0.386 ± 0.014
0.0MetXaa: 0.0 ± 0.0
Asn
3.071AsnAla: 3.071 ± 0.049
0.324AsnCys: 0.324 ± 0.014
1.388AsnAsp: 1.388 ± 0.041
1.392AsnGlu: 1.392 ± 0.034
0.976AsnPhe: 0.976 ± 0.028
2.176AsnGly: 2.176 ± 0.037
0.522AsnHis: 0.522 ± 0.019
1.196AsnIle: 1.196 ± 0.026
0.68AsnLys: 0.68 ± 0.023
2.76AsnLeu: 2.76 ± 0.042
0.555AsnMet: 0.555 ± 0.019
0.671AsnAsn: 0.671 ± 0.023
1.874AsnPro: 1.874 ± 0.036
0.776AsnGln: 0.776 ± 0.026
1.978AsnArg: 1.978 ± 0.038
1.173AsnSer: 1.173 ± 0.032
1.277AsnThr: 1.277 ± 0.033
1.955AsnVal: 1.955 ± 0.045
0.408AsnTrp: 0.408 ± 0.018
0.674AsnTyr: 0.674 ± 0.023
0.0AsnXaa: 0.0 ± 0.0
Pro
6.83ProAla: 6.83 ± 0.08
0.449ProCys: 0.449 ± 0.018
3.503ProAsp: 3.503 ± 0.053
3.71ProGlu: 3.71 ± 0.048
1.853ProPhe: 1.853 ± 0.031
4.514ProGly: 4.514 ± 0.063
1.131ProHis: 1.131 ± 0.026
1.937ProIle: 1.937 ± 0.038
1.491ProLys: 1.491 ± 0.034
5.095ProLeu: 5.095 ± 0.062
1.135ProMet: 1.135 ± 0.03
1.213ProAsn: 1.213 ± 0.025
2.604ProPro: 2.604 ± 0.057
1.76ProGln: 1.76 ± 0.036
3.121ProArg: 3.121 ± 0.05
2.317ProSer: 2.317 ± 0.038
2.377ProThr: 2.377 ± 0.038
4.342ProVal: 4.342 ± 0.063
0.681ProTrp: 0.681 ± 0.023
1.152ProTyr: 1.152 ± 0.028
0.0ProXaa: 0.0 ± 0.0
Gln
4.211GlnAla: 4.211 ± 0.062
0.295GlnCys: 0.295 ± 0.016
1.429GlnAsp: 1.429 ± 0.032
1.581GlnGlu: 1.581 ± 0.037
1.149GlnPhe: 1.149 ± 0.03
2.488GlnGly: 2.488 ± 0.041
0.792GlnHis: 0.792 ± 0.023
1.636GlnIle: 1.636 ± 0.036
1.025GlnLys: 1.025 ± 0.031
3.567GlnLeu: 3.567 ± 0.061
0.869GlnMet: 0.869 ± 0.025
0.764GlnAsn: 0.764 ± 0.023
1.758GlnPro: 1.758 ± 0.037
1.418GlnGln: 1.418 ± 0.035
2.831GlnArg: 2.831 ± 0.049
1.603GlnSer: 1.603 ± 0.031
1.577GlnThr: 1.577 ± 0.033
2.497GlnVal: 2.497 ± 0.043
0.502GlnTrp: 0.502 ± 0.019
0.706GlnTyr: 0.706 ± 0.023
0.0GlnXaa: 0.0 ± 0.0
Arg
8.24ArgAla: 8.24 ± 0.078
0.717ArgCys: 0.717 ± 0.022
4.291ArgAsp: 4.291 ± 0.054
5.064ArgGlu: 5.064 ± 0.061
3.127ArgPhe: 3.127 ± 0.048
5.08ArgGly: 5.08 ± 0.063
2.037ArgHis: 2.037 ± 0.035
4.118ArgIle: 4.118 ± 0.054
2.284ArgLys: 2.284 ± 0.04
8.549ArgLeu: 8.549 ± 0.099
1.999ArgMet: 1.999 ± 0.036
2.078ArgAsn: 2.078 ± 0.038
3.565ArgPro: 3.565 ± 0.052
2.737ArgGln: 2.737 ± 0.052
5.992ArgArg: 5.992 ± 0.074
3.716ArgSer: 3.716 ± 0.059
3.374ArgThr: 3.374 ± 0.047
5.568ArgVal: 5.568 ± 0.063
1.127ArgTrp: 1.127 ± 0.025
2.01ArgTyr: 2.01 ± 0.036
0.0ArgXaa: 0.0 ± 0.0
Ser
6.058SerAla: 6.058 ± 0.064
0.507SerCys: 0.507 ± 0.021
2.712SerAsp: 2.712 ± 0.045
2.653SerGlu: 2.653 ± 0.044
1.963SerPhe: 1.963 ± 0.04
5.249SerGly: 5.249 ± 0.067
1.082SerHis: 1.082 ± 0.026
2.296SerIle: 2.296 ± 0.041
1.334SerLys: 1.334 ± 0.031
5.457SerLeu: 5.457 ± 0.063
1.195SerMet: 1.195 ± 0.026
1.275SerAsn: 1.275 ± 0.032
2.716SerPro: 2.716 ± 0.041
1.498SerGln: 1.498 ± 0.033
3.667SerArg: 3.667 ± 0.049
2.67SerSer: 2.67 ± 0.048
2.521SerThr: 2.521 ± 0.043
3.926SerVal: 3.926 ± 0.05
0.695SerTrp: 0.695 ± 0.019
1.148SerTyr: 1.148 ± 0.031
0.0SerXaa: 0.0 ± 0.0
Thr
5.735ThrAla: 5.735 ± 0.078
0.472ThrCys: 0.472 ± 0.02
2.579ThrAsp: 2.579 ± 0.046
2.577ThrGlu: 2.577 ± 0.039
1.714ThrPhe: 1.714 ± 0.05
4.701ThrGly: 4.701 ± 0.078
1.101ThrHis: 1.101 ± 0.026
2.325ThrIle: 2.325 ± 0.044
1.201ThrLys: 1.201 ± 0.028
5.96ThrLeu: 5.96 ± 0.065
1.089ThrMet: 1.089 ± 0.025
1.255ThrAsn: 1.255 ± 0.036
3.171ThrPro: 3.171 ± 0.051
1.532ThrGln: 1.532 ± 0.037
3.505ThrArg: 3.505 ± 0.052
2.391ThrSer: 2.391 ± 0.043
2.644ThrThr: 2.644 ± 0.043
4.079ThrVal: 4.079 ± 0.053
0.65ThrTrp: 0.65 ± 0.023
1.038ThrTyr: 1.038 ± 0.029
0.0ThrXaa: 0.0 ± 0.0
Val
9.696ValAla: 9.696 ± 0.098
0.783ValCys: 0.783 ± 0.021
4.175ValAsp: 4.175 ± 0.06
4.649ValGlu: 4.649 ± 0.059
2.888ValPhe: 2.888 ± 0.048
5.773ValGly: 5.773 ± 0.065
1.547ValHis: 1.547 ± 0.028
3.728ValIle: 3.728 ± 0.053
2.358ValLys: 2.358 ± 0.044
8.018ValLeu: 8.018 ± 0.084
1.892ValMet: 1.892 ± 0.034
1.913ValAsn: 1.913 ± 0.037
3.868ValPro: 3.868 ± 0.057
2.257ValGln: 2.257 ± 0.04
5.618ValArg: 5.618 ± 0.06
4.256ValSer: 4.256 ± 0.055
4.127ValThr: 4.127 ± 0.056
6.538ValVal: 6.538 ± 0.076
0.951ValTrp: 0.951 ± 0.028
1.452ValTyr: 1.452 ± 0.032
0.0ValXaa: 0.0 ± 0.0
Trp
1.231TrpAla: 1.231 ± 0.026
0.159TrpCys: 0.159 ± 0.01
0.612TrpAsp: 0.612 ± 0.021
0.627TrpGlu: 0.627 ± 0.021
0.528TrpPhe: 0.528 ± 0.021
0.938TrpGly: 0.938 ± 0.025
0.387TrpHis: 0.387 ± 0.017
0.705TrpIle: 0.705 ± 0.023
0.484TrpLys: 0.484 ± 0.018
1.946TrpLeu: 1.946 ± 0.038
0.386TrpMet: 0.386 ± 0.017
0.424TrpAsn: 0.424 ± 0.018
0.626TrpPro: 0.626 ± 0.022
0.652TrpGln: 0.652 ± 0.021
1.202TrpArg: 1.202 ± 0.032
0.776TrpSer: 0.776 ± 0.024
0.621TrpThr: 0.621 ± 0.022
0.962TrpVal: 0.962 ± 0.026
0.25TrpTrp: 0.25 ± 0.014
0.312TrpTyr: 0.312 ± 0.016
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.592TyrAla: 2.592 ± 0.041
0.265TyrCys: 0.265 ± 0.012
1.28TyrAsp: 1.28 ± 0.031
1.122TyrGlu: 1.122 ± 0.026
0.943TyrPhe: 0.943 ± 0.027
2.016TyrGly: 2.016 ± 0.034
0.521TyrHis: 0.521 ± 0.018
0.809TyrIle: 0.809 ± 0.023
0.641TyrLys: 0.641 ± 0.021
2.479TyrLeu: 2.479 ± 0.04
0.43TyrMet: 0.43 ± 0.017
0.581TyrAsn: 0.581 ± 0.02
1.168TyrPro: 1.168 ± 0.029
0.785TyrGln: 0.785 ± 0.021
2.03TyrArg: 2.03 ± 0.038
1.133TyrSer: 1.133 ± 0.033
1.117TyrThr: 1.117 ± 0.031
1.721TyrVal: 1.721 ± 0.032
0.337TyrTrp: 0.337 ± 0.016
0.66TyrTyr: 0.66 ± 0.025
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4693 proteins (1569040 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski