Nesterenkonia sp. PF2B19

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Micrococcaceae; Nesterenkonia; unclassified Nesterenkonia

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2886 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X3PBF8|A0A1X3PBF8_9MICC Oxaloacetate decarboxylase OS=Nesterenkonia sp. PF2B19 OX=1881858 GN=BCY76_002105 PE=3 SV=1
MM1 pKa = 7.6TKK3 pKa = 10.55RR4 pKa = 11.84LTLTLGTAVFSATLFLAGCATDD26 pKa = 4.83SEE28 pKa = 5.01PDD30 pKa = 3.58PSDD33 pKa = 3.04TSAEE37 pKa = 4.22HH38 pKa = 6.25QDD40 pKa = 3.06HH41 pKa = 6.71GEE43 pKa = 4.0NSEE46 pKa = 4.01EE47 pKa = 4.24AEE49 pKa = 4.63DD50 pKa = 3.83GHH52 pKa = 6.55EE53 pKa = 4.74HH54 pKa = 6.5MDD56 pKa = 3.45HH57 pKa = 6.93PADD60 pKa = 4.2GGPVPEE66 pKa = 5.1GMTEE70 pKa = 3.94ATDD73 pKa = 3.56PEE75 pKa = 4.63YY76 pKa = 10.82QVGDD80 pKa = 4.27EE81 pKa = 4.27VTLTADD87 pKa = 4.19HH88 pKa = 6.46MPGMDD93 pKa = 3.54GATATIVGAYY103 pKa = 7.7DD104 pKa = 3.63TYY106 pKa = 10.77TYY108 pKa = 11.11SVDD111 pKa = 3.54YY112 pKa = 10.39TPTTGGDD119 pKa = 3.3PVTDD123 pKa = 3.68HH124 pKa = 6.6RR125 pKa = 11.84WVVHH129 pKa = 6.18EE130 pKa = 4.83EE131 pKa = 3.91IEE133 pKa = 4.65DD134 pKa = 3.65VGDD137 pKa = 3.47QRR139 pKa = 11.84LEE141 pKa = 4.5DD142 pKa = 3.79GTEE145 pKa = 3.82VTLAAAHH152 pKa = 5.81MEE154 pKa = 4.17GMDD157 pKa = 3.72GATATIFSSTEE168 pKa = 3.47EE169 pKa = 3.83TVYY172 pKa = 11.09VVDD175 pKa = 5.46FEE177 pKa = 6.02ADD179 pKa = 3.16GMTMTNHH186 pKa = 5.21KK187 pKa = 9.39WVVEE191 pKa = 4.08SEE193 pKa = 4.19IEE195 pKa = 4.08PTSS198 pKa = 3.32

Molecular weight: 21.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X3P5B9|A0A1X3P5B9_9MICC NLPC_P60 domain-containing protein OS=Nesterenkonia sp. PF2B19 OX=1881858 GN=BCY76_014415 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84SRR15 pKa = 11.84KK16 pKa = 8.87HH17 pKa = 4.83GFRR20 pKa = 11.84SRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84SIISARR35 pKa = 11.84RR36 pKa = 11.84SRR38 pKa = 11.84GRR40 pKa = 11.84KK41 pKa = 6.49TLSAA45 pKa = 4.22

Molecular weight: 5.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2886

0

2886

891083

32

1605

308.8

33.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.562 ± 0.055

0.648 ± 0.013

6.303 ± 0.048

6.72 ± 0.054

2.866 ± 0.032

8.979 ± 0.044

2.488 ± 0.024

4.011 ± 0.029

1.639 ± 0.03

10.214 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.096 ± 0.02

1.65 ± 0.023

5.633 ± 0.032

3.293 ± 0.026

7.514 ± 0.053

5.651 ± 0.027

5.94 ± 0.036

8.451 ± 0.04

1.517 ± 0.023

1.826 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski