Ruminococcaceae bacterium D5

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; unclassified Oscillospiraceae

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2495 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I3JXK1|A0A1I3JXK1_9FIRM N-acyl-D-amino-acid deacylase OS=Ruminococcaceae bacterium D5 OX=1520815 GN=SAMN02910435_00486 PE=4 SV=1
MM1 pKa = 7.88SEE3 pKa = 4.17LCNCGHH9 pKa = 5.33EE10 pKa = 4.6HH11 pKa = 5.32EE12 pKa = 5.54HH13 pKa = 6.45EE14 pKa = 4.25EE15 pKa = 4.44CGCGCGEE22 pKa = 4.0QEE24 pKa = 4.65GMDD27 pKa = 3.71GVVTLVDD34 pKa = 3.63EE35 pKa = 5.39DD36 pKa = 4.3GVEE39 pKa = 4.05HH40 pKa = 6.54GFEE43 pKa = 5.06IIDD46 pKa = 3.68MVEE49 pKa = 3.8LDD51 pKa = 3.42GAQYY55 pKa = 10.66VAVVPADD62 pKa = 3.89EE63 pKa = 4.73EE64 pKa = 4.44PEE66 pKa = 3.94EE67 pKa = 5.2DD68 pKa = 4.62DD69 pKa = 5.1EE70 pKa = 6.03SDD72 pKa = 3.56EE73 pKa = 4.69LVLLKK78 pKa = 10.57VVAEE82 pKa = 4.32GVEE85 pKa = 4.15EE86 pKa = 3.86FLEE89 pKa = 4.99AIEE92 pKa = 5.0SEE94 pKa = 4.5EE95 pKa = 3.8EE96 pKa = 3.96FNRR99 pKa = 11.84VSAVFMEE106 pKa = 4.28RR107 pKa = 11.84LKK109 pKa = 11.14DD110 pKa = 3.86DD111 pKa = 3.69YY112 pKa = 11.71DD113 pKa = 3.75FVEE116 pKa = 4.83GEE118 pKa = 4.12EE119 pKa = 4.11

Molecular weight: 13.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I3R3I2|A0A1I3R3I2_9FIRM Coat F domain-containing protein OS=Ruminococcaceae bacterium D5 OX=1520815 GN=SAMN02910435_01971 PE=4 SV=1
MM1 pKa = 7.4KK2 pKa = 10.24NSRR5 pKa = 11.84KK6 pKa = 10.17AIGLAACLAVLLLLLGALPRR26 pKa = 11.84RR27 pKa = 11.84EE28 pKa = 3.9QWLSLARR35 pKa = 11.84DD36 pKa = 3.37PDD38 pKa = 4.31LIRR41 pKa = 11.84RR42 pKa = 11.84FTGWIDD48 pKa = 3.83SIGSAGIPVLLLFQIFQIVVAILPGGLVEE77 pKa = 4.26LAGGILYY84 pKa = 10.56GPLGGFAVALFGILLGTLCAFWISRR109 pKa = 11.84RR110 pKa = 11.84FGEE113 pKa = 4.05PLVRR117 pKa = 11.84RR118 pKa = 11.84LVNGKK123 pKa = 9.19QFDD126 pKa = 4.02RR127 pKa = 11.84YY128 pKa = 10.47KK129 pKa = 10.51KK130 pKa = 9.05LCASRR135 pKa = 11.84RR136 pKa = 11.84FEE138 pKa = 4.03AAALLFFFLPGVPKK152 pKa = 10.57DD153 pKa = 3.23ILIYY157 pKa = 10.38TIGCSGVLSRR167 pKa = 11.84RR168 pKa = 11.84FILHH172 pKa = 5.78ATLARR177 pKa = 11.84IPSVAVSCFAGSVAGKK193 pKa = 10.83GDD195 pKa = 3.71FLLFTVIYY203 pKa = 10.15AAFLAAGGIGTLVHH217 pKa = 6.54RR218 pKa = 11.84RR219 pKa = 11.84ILRR222 pKa = 11.84RR223 pKa = 11.84LEE225 pKa = 4.04QKK227 pKa = 10.48EE228 pKa = 4.03KK229 pKa = 11.12

Molecular weight: 24.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2495

0

2495

741156

39

1780

297.1

32.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.117 ± 0.065

1.752 ± 0.025

5.018 ± 0.042

6.767 ± 0.055

4.004 ± 0.034

8.176 ± 0.05

1.695 ± 0.02

6.028 ± 0.042

4.304 ± 0.042

10.308 ± 0.068

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.809 ± 0.029

3.06 ± 0.031

4.42 ± 0.034

3.124 ± 0.027

6.294 ± 0.06

6.132 ± 0.041

5.012 ± 0.041

6.925 ± 0.04

0.86 ± 0.018

3.192 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski