Amino acid dipepetide frequency for Ruminococcaceae bacterium D5

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.182AlaAla: 14.182 ± 0.208
1.728AlaCys: 1.728 ± 0.05
5.018AlaAsp: 5.018 ± 0.077
6.516AlaGlu: 6.516 ± 0.116
3.758AlaPhe: 3.758 ± 0.072
8.813AlaGly: 8.813 ± 0.13
1.422AlaHis: 1.422 ± 0.042
5.011AlaIle: 5.011 ± 0.089
4.212AlaLys: 4.212 ± 0.091
11.069AlaLeu: 11.069 ± 0.177
2.751AlaMet: 2.751 ± 0.067
2.504AlaAsn: 2.504 ± 0.062
3.983AlaPro: 3.983 ± 0.105
3.569AlaGln: 3.569 ± 0.074
5.621AlaArg: 5.621 ± 0.103
5.27AlaSer: 5.27 ± 0.101
3.511AlaThr: 3.511 ± 0.073
8.999AlaVal: 8.999 ± 0.141
0.633AlaTrp: 0.633 ± 0.03
2.599AlaTyr: 2.599 ± 0.074
0.0AlaXaa: 0.0 ± 0.0
Cys
1.815CysAla: 1.815 ± 0.053
0.498CysCys: 0.498 ± 0.031
0.926CysAsp: 0.926 ± 0.038
1.051CysGlu: 1.051 ± 0.038
0.741CysPhe: 0.741 ± 0.033
2.175CysGly: 2.175 ± 0.062
0.34CysHis: 0.34 ± 0.021
0.95CysIle: 0.95 ± 0.035
0.583CysLys: 0.583 ± 0.03
1.378CysLeu: 1.378 ± 0.043
0.389CysMet: 0.389 ± 0.025
0.48CysAsn: 0.48 ± 0.028
0.881CysPro: 0.881 ± 0.034
0.335CysGln: 0.335 ± 0.02
1.325CysArg: 1.325 ± 0.05
1.127CysSer: 1.127 ± 0.039
0.753CysThr: 0.753 ± 0.034
1.051CysVal: 1.051 ± 0.034
0.148CysTrp: 0.148 ± 0.012
0.576CysTyr: 0.576 ± 0.04
0.0CysXaa: 0.0 ± 0.0
Asp
4.875AspAla: 4.875 ± 0.09
0.834AspCys: 0.834 ± 0.041
2.418AspAsp: 2.418 ± 0.086
3.698AspGlu: 3.698 ± 0.078
2.275AspPhe: 2.275 ± 0.052
4.546AspGly: 4.546 ± 0.083
0.866AspHis: 0.866 ± 0.038
2.958AspIle: 2.958 ± 0.073
1.799AspLys: 1.799 ± 0.058
5.035AspLeu: 5.035 ± 0.086
1.255AspMet: 1.255 ± 0.048
1.302AspAsn: 1.302 ± 0.05
2.757AspPro: 2.757 ± 0.073
1.324AspGln: 1.324 ± 0.047
3.466AspArg: 3.466 ± 0.069
2.852AspSer: 2.852 ± 0.081
2.408AspThr: 2.408 ± 0.065
2.994AspVal: 2.994 ± 0.072
0.548AspTrp: 0.548 ± 0.027
1.974AspTyr: 1.974 ± 0.054
0.0AspXaa: 0.0 ± 0.0
Glu
6.529GluAla: 6.529 ± 0.107
0.704GluCys: 0.704 ± 0.032
2.953GluAsp: 2.953 ± 0.065
5.429GluGlu: 5.429 ± 0.108
2.052GluPhe: 2.052 ± 0.06
4.833GluGly: 4.833 ± 0.098
1.337GluHis: 1.337 ± 0.047
4.659GluIle: 4.659 ± 0.088
3.987GluLys: 3.987 ± 0.084
6.916GluLeu: 6.916 ± 0.109
2.071GluMet: 2.071 ± 0.056
2.631GluAsn: 2.631 ± 0.07
2.396GluPro: 2.396 ± 0.055
3.14GluGln: 3.14 ± 0.072
4.86GluArg: 4.86 ± 0.11
3.237GluSer: 3.237 ± 0.066
3.401GluThr: 3.401 ± 0.074
3.88GluVal: 3.88 ± 0.078
0.522GluTrp: 0.522 ± 0.028
2.129GluTyr: 2.129 ± 0.057
0.0GluXaa: 0.0 ± 0.0
Phe
3.86PheAla: 3.86 ± 0.079
0.988PheCys: 0.988 ± 0.039
2.38PheAsp: 2.38 ± 0.053
2.619PheGlu: 2.619 ± 0.061
1.904PhePhe: 1.904 ± 0.063
3.574PheGly: 3.574 ± 0.063
0.667PheHis: 0.667 ± 0.032
1.956PheIle: 1.956 ± 0.065
1.129PheLys: 1.129 ± 0.041
4.307PheLeu: 4.307 ± 0.092
0.903PheMet: 0.903 ± 0.035
0.989PheAsn: 0.989 ± 0.036
1.711PhePro: 1.711 ± 0.05
0.982PheGln: 0.982 ± 0.036
1.917PheArg: 1.917 ± 0.057
3.326PheSer: 3.326 ± 0.067
2.303PheThr: 2.303 ± 0.06
2.724PheVal: 2.724 ± 0.068
0.426PheTrp: 0.426 ± 0.027
1.374PheTyr: 1.374 ± 0.043
0.0PheXaa: 0.0 ± 0.0
Gly
7.571GlyAla: 7.571 ± 0.125
1.728GlyCys: 1.728 ± 0.06
3.571GlyAsp: 3.571 ± 0.07
5.906GlyGlu: 5.906 ± 0.126
3.449GlyPhe: 3.449 ± 0.065
6.698GlyGly: 6.698 ± 0.123
1.386GlyHis: 1.386 ± 0.044
5.791GlyIle: 5.791 ± 0.099
4.103GlyLys: 4.103 ± 0.075
7.643GlyLeu: 7.643 ± 0.114
2.81GlyMet: 2.81 ± 0.076
2.365GlyAsn: 2.365 ± 0.064
2.12GlyPro: 2.12 ± 0.058
2.394GlyGln: 2.394 ± 0.058
5.181GlyArg: 5.181 ± 0.104
4.857GlySer: 4.857 ± 0.09
4.195GlyThr: 4.195 ± 0.087
6.077GlyVal: 6.077 ± 0.097
0.904GlyTrp: 0.904 ± 0.039
3.016GlyTyr: 3.016 ± 0.059
0.0GlyXaa: 0.0 ± 0.0
His
1.392HisAla: 1.392 ± 0.045
0.393HisCys: 0.393 ± 0.025
0.823HisAsp: 0.823 ± 0.037
0.928HisGlu: 0.928 ± 0.033
0.746HisPhe: 0.746 ± 0.029
1.268HisGly: 1.268 ± 0.043
0.41HisHis: 0.41 ± 0.025
1.044HisIle: 1.044 ± 0.041
0.582HisLys: 0.582 ± 0.027
1.641HisLeu: 1.641 ± 0.051
0.412HisMet: 0.412 ± 0.023
0.584HisAsn: 0.584 ± 0.026
1.204HisPro: 1.204 ± 0.037
0.544HisGln: 0.544 ± 0.027
1.228HisArg: 1.228 ± 0.045
1.054HisSer: 1.054 ± 0.04
0.99HisThr: 0.99 ± 0.04
0.843HisVal: 0.843 ± 0.035
0.182HisTrp: 0.182 ± 0.015
0.681HisTyr: 0.681 ± 0.03
0.0HisXaa: 0.0 ± 0.0
Ile
6.155IleAla: 6.155 ± 0.096
1.136IleCys: 1.136 ± 0.04
3.654IleAsp: 3.654 ± 0.081
4.076IleGlu: 4.076 ± 0.079
2.321IlePhe: 2.321 ± 0.068
5.11IleGly: 5.11 ± 0.09
1.032IleHis: 1.032 ± 0.041
3.33IleIle: 3.33 ± 0.081
2.171IleLys: 2.171 ± 0.064
5.927IleLeu: 5.927 ± 0.104
1.341IleMet: 1.341 ± 0.055
1.921IleAsn: 1.921 ± 0.06
3.19IlePro: 3.19 ± 0.064
1.454IleGln: 1.454 ± 0.045
3.601IleArg: 3.601 ± 0.072
4.158IleSer: 4.158 ± 0.072
3.387IleThr: 3.387 ± 0.074
4.162IleVal: 4.162 ± 0.081
0.474IleTrp: 0.474 ± 0.025
1.684IleTyr: 1.684 ± 0.049
0.0IleXaa: 0.0 ± 0.0
Lys
3.998LysAla: 3.998 ± 0.09
0.437LysCys: 0.437 ± 0.029
2.028LysAsp: 2.028 ± 0.063
3.129LysGlu: 3.129 ± 0.083
1.287LysPhe: 1.287 ± 0.045
3.083LysGly: 3.083 ± 0.071
0.622LysHis: 0.622 ± 0.029
2.922LysIle: 2.922 ± 0.073
3.001LysLys: 3.001 ± 0.081
3.922LysLeu: 3.922 ± 0.078
1.433LysMet: 1.433 ± 0.046
1.915LysAsn: 1.915 ± 0.059
1.718LysPro: 1.718 ± 0.057
1.432LysGln: 1.432 ± 0.049
2.682LysArg: 2.682 ± 0.054
2.396LysSer: 2.396 ± 0.064
2.577LysThr: 2.577 ± 0.054
2.715LysVal: 2.715 ± 0.065
0.306LysTrp: 0.306 ± 0.022
1.436LysTyr: 1.436 ± 0.053
0.001LysXaa: 0.001 ± 0.002
Leu
10.386LeuAla: 10.386 ± 0.16
2.214LeuCys: 2.214 ± 0.068
5.363LeuAsp: 5.363 ± 0.089
6.352LeuGlu: 6.352 ± 0.101
4.401LeuPhe: 4.401 ± 0.101
7.661LeuGly: 7.661 ± 0.117
1.664LeuHis: 1.664 ± 0.054
5.769LeuIle: 5.769 ± 0.095
3.938LeuLys: 3.938 ± 0.079
12.08LeuLeu: 12.08 ± 0.186
2.779LeuMet: 2.779 ± 0.073
2.81LeuAsn: 2.81 ± 0.069
5.242LeuPro: 5.242 ± 0.094
2.507LeuGln: 2.507 ± 0.06
6.638LeuArg: 6.638 ± 0.11
7.737LeuSer: 7.737 ± 0.13
5.647LeuThr: 5.647 ± 0.101
6.093LeuVal: 6.093 ± 0.095
0.892LeuTrp: 0.892 ± 0.035
2.91LeuTyr: 2.91 ± 0.068
0.0LeuXaa: 0.0 ± 0.0
Met
2.697MetAla: 2.697 ± 0.068
0.281MetCys: 0.281 ± 0.021
1.139MetAsp: 1.139 ± 0.043
1.711MetGlu: 1.711 ± 0.054
0.792MetPhe: 0.792 ± 0.031
2.23MetGly: 2.23 ± 0.061
0.428MetHis: 0.428 ± 0.02
1.904MetIle: 1.904 ± 0.056
1.78MetLys: 1.78 ± 0.046
3.182MetLeu: 3.182 ± 0.075
0.946MetMet: 0.946 ± 0.04
1.075MetAsn: 1.075 ± 0.045
1.355MetPro: 1.355 ± 0.037
1.048MetGln: 1.048 ± 0.04
1.534MetArg: 1.534 ± 0.048
1.657MetSer: 1.657 ± 0.054
1.661MetThr: 1.661 ± 0.048
1.911MetVal: 1.911 ± 0.051
0.174MetTrp: 0.174 ± 0.015
0.583MetTyr: 0.583 ± 0.03
0.0MetXaa: 0.0 ± 0.0
Asn
2.901AsnAla: 2.901 ± 0.064
0.503AsnCys: 0.503 ± 0.024
1.413AsnAsp: 1.413 ± 0.05
1.734AsnGlu: 1.734 ± 0.051
1.194AsnPhe: 1.194 ± 0.04
2.685AsnGly: 2.685 ± 0.07
0.571AsnHis: 0.571 ± 0.029
2.023AsnIle: 2.023 ± 0.053
1.185AsnLys: 1.185 ± 0.044
3.075AsnLeu: 3.075 ± 0.064
0.792AsnMet: 0.792 ± 0.035
0.931AsnAsn: 0.931 ± 0.041
1.987AsnPro: 1.987 ± 0.049
0.95AsnGln: 0.95 ± 0.041
2.071AsnArg: 2.071 ± 0.054
1.685AsnSer: 1.685 ± 0.052
1.638AsnThr: 1.638 ± 0.056
1.921AsnVal: 1.921 ± 0.054
0.274AsnTrp: 0.274 ± 0.019
1.065AsnTyr: 1.065 ± 0.042
0.0AsnXaa: 0.0 ± 0.0
Pro
5.103ProAla: 5.103 ± 0.131
0.694ProCys: 0.694 ± 0.032
2.766ProAsp: 2.766 ± 0.064
3.682ProGlu: 3.682 ± 0.078
1.836ProPhe: 1.836 ± 0.048
3.632ProGly: 3.632 ± 0.089
0.808ProHis: 0.808 ± 0.033
2.253ProIle: 2.253 ± 0.06
1.765ProLys: 1.765 ± 0.051
4.131ProLeu: 4.131 ± 0.086
1.183ProMet: 1.183 ± 0.041
1.218ProAsn: 1.218 ± 0.041
1.73ProPro: 1.73 ± 0.046
1.494ProGln: 1.494 ± 0.051
2.054ProArg: 2.054 ± 0.057
2.503ProSer: 2.503 ± 0.057
1.811ProThr: 1.811 ± 0.05
3.816ProVal: 3.816 ± 0.07
0.341ProTrp: 0.341 ± 0.019
1.38ProTyr: 1.38 ± 0.047
0.0ProXaa: 0.0 ± 0.0
Gln
2.944GlnAla: 2.944 ± 0.06
0.372GlnCys: 0.372 ± 0.023
1.326GlnAsp: 1.326 ± 0.039
2.233GlnGlu: 2.233 ± 0.067
1.025GlnPhe: 1.025 ± 0.038
2.135GlnGly: 2.135 ± 0.053
0.457GlnHis: 0.457 ± 0.028
2.256GlnIle: 2.256 ± 0.057
2.025GlnLys: 2.025 ± 0.063
2.971GlnLeu: 2.971 ± 0.061
1.019GlnMet: 1.019 ± 0.037
1.363GlnAsn: 1.363 ± 0.049
1.152GlnPro: 1.152 ± 0.045
1.298GlnGln: 1.298 ± 0.047
2.068GlnArg: 2.068 ± 0.063
1.788GlnSer: 1.788 ± 0.056
1.526GlnThr: 1.526 ± 0.046
1.931GlnVal: 1.931 ± 0.057
0.239GlnTrp: 0.239 ± 0.02
1.113GlnTyr: 1.113 ± 0.044
0.0GlnXaa: 0.0 ± 0.0
Arg
5.504ArgAla: 5.504 ± 0.105
1.197ArgCys: 1.197 ± 0.045
2.835ArgAsp: 2.835 ± 0.066
5.139ArgGlu: 5.139 ± 0.103
2.73ArgPhe: 2.73 ± 0.07
4.307ArgGly: 4.307 ± 0.086
1.135ArgHis: 1.135 ± 0.043
4.102ArgIle: 4.102 ± 0.077
2.528ArgLys: 2.528 ± 0.059
6.551ArgLeu: 6.551 ± 0.132
2.02ArgMet: 2.02 ± 0.052
1.763ArgAsn: 1.763 ± 0.055
2.541ArgPro: 2.541 ± 0.067
2.259ArgGln: 2.259 ± 0.055
5.235ArgArg: 5.235 ± 0.127
3.504ArgSer: 3.504 ± 0.078
2.932ArgThr: 2.932 ± 0.063
3.768ArgVal: 3.768 ± 0.073
0.63ArgTrp: 0.63 ± 0.033
2.265ArgTyr: 2.265 ± 0.069
0.0ArgXaa: 0.0 ± 0.0
Ser
6.277SerAla: 6.277 ± 0.101
1.028SerCys: 1.028 ± 0.037
3.11SerAsp: 3.11 ± 0.076
3.71SerGlu: 3.71 ± 0.079
2.831SerPhe: 2.831 ± 0.059
5.899SerGly: 5.899 ± 0.105
1.083SerHis: 1.083 ± 0.041
3.555SerIle: 3.555 ± 0.073
2.236SerLys: 2.236 ± 0.054
6.251SerLeu: 6.251 ± 0.1
1.77SerMet: 1.77 ± 0.05
1.697SerAsn: 1.697 ± 0.063
2.755SerPro: 2.755 ± 0.062
1.857SerGln: 1.857 ± 0.054
3.581SerArg: 3.581 ± 0.074
4.032SerSer: 4.032 ± 0.121
2.75SerThr: 2.75 ± 0.063
4.322SerVal: 4.322 ± 0.073
0.582SerTrp: 0.582 ± 0.034
2.0SerTyr: 2.0 ± 0.062
0.0SerXaa: 0.0 ± 0.0
Thr
5.626ThrAla: 5.626 ± 0.097
0.658ThrCys: 0.658 ± 0.03
2.589ThrAsp: 2.589 ± 0.064
2.819ThrGlu: 2.819 ± 0.069
1.935ThrPhe: 1.935 ± 0.059
5.014ThrGly: 5.014 ± 0.084
0.796ThrHis: 0.796 ± 0.035
3.007ThrIle: 3.007 ± 0.074
1.736ThrLys: 1.736 ± 0.055
5.338ThrLeu: 5.338 ± 0.097
1.29ThrMet: 1.29 ± 0.047
1.376ThrAsn: 1.376 ± 0.05
2.661ThrPro: 2.661 ± 0.059
1.274ThrGln: 1.274 ± 0.047
2.678ThrArg: 2.678 ± 0.057
2.527ThrSer: 2.527 ± 0.06
2.43ThrThr: 2.43 ± 0.06
4.722ThrVal: 4.722 ± 0.086
0.314ThrTrp: 0.314 ± 0.023
1.333ThrTyr: 1.333 ± 0.043
0.0ThrXaa: 0.0 ± 0.0
Val
5.871ValAla: 5.871 ± 0.103
1.398ValCys: 1.398 ± 0.047
3.473ValAsp: 3.473 ± 0.069
4.289ValGlu: 4.289 ± 0.076
2.924ValPhe: 2.924 ± 0.065
4.894ValGly: 4.894 ± 0.111
1.071ValHis: 1.071 ± 0.039
4.571ValIle: 4.571 ± 0.092
2.792ValLys: 2.792 ± 0.07
7.379ValLeu: 7.379 ± 0.091
2.002ValMet: 2.002 ± 0.065
2.194ValAsn: 2.194 ± 0.062
3.064ValPro: 3.064 ± 0.066
1.906ValGln: 1.906 ± 0.049
4.253ValArg: 4.253 ± 0.079
5.259ValSer: 5.259 ± 0.092
3.998ValThr: 3.998 ± 0.094
5.045ValVal: 5.045 ± 0.092
0.668ValTrp: 0.668 ± 0.031
2.203ValTyr: 2.203 ± 0.061
0.0ValXaa: 0.0 ± 0.0
Trp
0.689TrpAla: 0.689 ± 0.034
0.147TrpCys: 0.147 ± 0.015
0.432TrpAsp: 0.432 ± 0.026
0.623TrpGlu: 0.623 ± 0.027
0.341TrpPhe: 0.341 ± 0.024
0.675TrpGly: 0.675 ± 0.033
0.167TrpHis: 0.167 ± 0.017
0.534TrpIle: 0.534 ± 0.023
0.439TrpLys: 0.439 ± 0.024
0.978TrpLeu: 0.978 ± 0.042
0.325TrpMet: 0.325 ± 0.019
0.375TrpAsn: 0.375 ± 0.025
0.246TrpPro: 0.246 ± 0.016
0.329TrpGln: 0.329 ± 0.019
0.536TrpArg: 0.536 ± 0.025
0.49TrpSer: 0.49 ± 0.029
0.378TrpThr: 0.378 ± 0.023
0.48TrpVal: 0.48 ± 0.025
0.112TrpTrp: 0.112 ± 0.014
0.305TrpTyr: 0.305 ± 0.021
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.794TyrAla: 2.794 ± 0.064
0.582TyrCys: 0.582 ± 0.029
1.966TyrAsp: 1.966 ± 0.059
2.024TyrGlu: 2.024 ± 0.058
1.498TyrPhe: 1.498 ± 0.048
2.533TyrGly: 2.533 ± 0.063
0.687TyrHis: 0.687 ± 0.03
1.688TyrIle: 1.688 ± 0.054
1.05TyrLys: 1.05 ± 0.043
3.311TyrLeu: 3.311 ± 0.071
0.595TyrMet: 0.595 ± 0.026
1.101TyrAsn: 1.101 ± 0.035
1.51TyrPro: 1.51 ± 0.054
1.148TyrGln: 1.148 ± 0.042
2.414TyrArg: 2.414 ± 0.067
1.866TyrSer: 1.866 ± 0.05
1.83TyrThr: 1.83 ± 0.067
1.799TyrVal: 1.799 ± 0.049
0.232TyrTrp: 0.232 ± 0.017
1.295TyrTyr: 1.295 ± 0.052
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.001XaaGly: 0.001 ± 0.002
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.004XaaXaa: 0.004 ± 0.005
Statistics based on 2495 proteins (741156 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski