Bacillus phage SP-10

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Spounavirinae; unclassified Spounavirinae

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 236 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F8WQ79|F8WQ79_9CAUD Uncharacterized protein OS=Bacillus phage SP-10 OX=941058 PE=4 SV=1
MM1 pKa = 7.84VYY3 pKa = 10.49DD4 pKa = 3.98GLAAIVGAKK13 pKa = 9.01VVEE16 pKa = 4.44VSEE19 pKa = 4.04HH20 pKa = 6.93LIVFDD25 pKa = 3.22NGIRR29 pKa = 11.84IEE31 pKa = 4.35CEE33 pKa = 3.72DD34 pKa = 3.61VEE36 pKa = 5.13SYY38 pKa = 11.67LNVSEE43 pKa = 4.64YY44 pKa = 10.77EE45 pKa = 3.95AGEE48 pKa = 3.98

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F8WPT3|F8WPT3_9CAUD Uncharacterized protein OS=Bacillus phage SP-10 OX=941058 PE=4 SV=1
MM1 pKa = 7.54KK2 pKa = 9.12PACAVSFKK10 pKa = 10.31TRR12 pKa = 11.84YY13 pKa = 7.56RR14 pKa = 11.84CYY16 pKa = 9.7YY17 pKa = 7.6VTGRR21 pKa = 11.84TIDD24 pKa = 3.01VDD26 pKa = 3.56KK27 pKa = 10.57RR28 pKa = 11.84VHH30 pKa = 6.66IGTLKK35 pKa = 10.14IKK37 pKa = 10.41EE38 pKa = 3.81NAEE41 pKa = 4.26RR42 pKa = 11.84IRR44 pKa = 11.84EE45 pKa = 4.0LVLAGNKK52 pKa = 9.14LDD54 pKa = 3.76KK55 pKa = 10.99VRR57 pKa = 11.84EE58 pKa = 3.81EE59 pKa = 3.9LARR62 pKa = 11.84LKK64 pKa = 10.7LIEE67 pKa = 4.61RR68 pKa = 11.84RR69 pKa = 11.84DD70 pKa = 3.42PSLRR74 pKa = 11.84RR75 pKa = 11.84SKK77 pKa = 10.9YY78 pKa = 8.54IYY80 pKa = 9.77KK81 pKa = 10.56GKK83 pKa = 8.68TGYY86 pKa = 10.5QVMFISKK93 pKa = 9.61GKK95 pKa = 10.02ARR97 pKa = 11.84SRR99 pKa = 11.84TAKK102 pKa = 9.21TLEE105 pKa = 3.97EE106 pKa = 3.88ARR108 pKa = 11.84QIRR111 pKa = 11.84DD112 pKa = 3.48FFVKK116 pKa = 10.61NGFFPPKK123 pKa = 8.76RR124 pKa = 11.84KK125 pKa = 9.57EE126 pKa = 3.44YY127 pKa = 10.2RR128 pKa = 11.84AYY130 pKa = 9.6IWFNRR135 pKa = 11.84HH136 pKa = 4.65FKK138 pKa = 10.15GGRR141 pKa = 11.84YY142 pKa = 8.76CVQHH146 pKa = 6.31HH147 pKa = 6.06KK148 pKa = 11.01VILSYY153 pKa = 10.08CTSLDD158 pKa = 3.31EE159 pKa = 4.36ATRR162 pKa = 11.84ILNQVNSMKK171 pKa = 10.71AEE173 pKa = 4.27EE174 pKa = 4.12IEE176 pKa = 3.99ALKK179 pKa = 10.97EE180 pKa = 3.73NVKK183 pKa = 10.18RR184 pKa = 11.84RR185 pKa = 11.84RR186 pKa = 11.84KK187 pKa = 9.69RR188 pKa = 11.84GGKK191 pKa = 8.83RR192 pKa = 11.84RR193 pKa = 11.84LPEE196 pKa = 3.99GVFEE200 pKa = 5.6DD201 pKa = 4.02KK202 pKa = 10.36MSPGLLWVSRR212 pKa = 3.96

Molecular weight: 25.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

236

0

236

43986

40

1500

186.4

21.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.711 ± 0.213

0.916 ± 0.064

6.579 ± 0.146

8.762 ± 0.218

3.954 ± 0.127

6.232 ± 0.196

1.585 ± 0.088

5.934 ± 0.134

8.737 ± 0.228

8.564 ± 0.166

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.453 ± 0.078

4.865 ± 0.125

3.074 ± 0.112

3.124 ± 0.116

4.908 ± 0.149

6.088 ± 0.121

5.997 ± 0.177

7.316 ± 0.176

1.169 ± 0.073

4.033 ± 0.118
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski