Amino acid dipepetide frequency for Bacillus phage SP-10

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.046AlaAla: 3.046 ± 0.353
0.659AlaCys: 0.659 ± 0.123
3.569AlaAsp: 3.569 ± 0.31
4.433AlaGlu: 4.433 ± 0.331
2.546AlaPhe: 2.546 ± 0.239
3.91AlaGly: 3.91 ± 0.368
0.909AlaHis: 0.909 ± 0.137
3.865AlaIle: 3.865 ± 0.341
5.752AlaLys: 5.752 ± 0.388
5.479AlaLeu: 5.479 ± 0.326
1.432AlaMet: 1.432 ± 0.188
2.524AlaAsn: 2.524 ± 0.258
1.341AlaPro: 1.341 ± 0.183
1.523AlaGln: 1.523 ± 0.177
2.615AlaArg: 2.615 ± 0.223
3.206AlaSer: 3.206 ± 0.255
3.388AlaThr: 3.388 ± 0.294
4.092AlaVal: 4.092 ± 0.299
0.591AlaTrp: 0.591 ± 0.114
2.228AlaTyr: 2.228 ± 0.253
0.0AlaXaa: 0.0 ± 0.0
Cys
0.386CysAla: 0.386 ± 0.09
0.114CysCys: 0.114 ± 0.053
0.5CysAsp: 0.5 ± 0.093
0.728CysGlu: 0.728 ± 0.141
0.477CysPhe: 0.477 ± 0.11
0.546CysGly: 0.546 ± 0.123
0.159CysHis: 0.159 ± 0.067
0.432CysIle: 0.432 ± 0.098
0.978CysLys: 0.978 ± 0.147
0.637CysLeu: 0.637 ± 0.128
0.25CysMet: 0.25 ± 0.073
0.455CysAsn: 0.455 ± 0.093
0.409CysPro: 0.409 ± 0.096
0.25CysGln: 0.25 ± 0.084
0.296CysArg: 0.296 ± 0.089
0.75CysSer: 0.75 ± 0.125
0.591CysThr: 0.591 ± 0.102
0.637CysVal: 0.637 ± 0.111
0.136CysTrp: 0.136 ± 0.056
0.432CysTyr: 0.432 ± 0.08
0.0CysXaa: 0.0 ± 0.0
Asp
4.001AspAla: 4.001 ± 0.323
0.5AspCys: 0.5 ± 0.107
4.433AspAsp: 4.433 ± 0.514
6.661AspGlu: 6.661 ± 0.552
3.16AspPhe: 3.16 ± 0.291
4.433AspGly: 4.433 ± 0.425
0.841AspHis: 0.841 ± 0.136
3.706AspIle: 3.706 ± 0.255
4.342AspLys: 4.342 ± 0.327
5.957AspLeu: 5.957 ± 0.331
1.91AspMet: 1.91 ± 0.189
3.228AspAsn: 3.228 ± 0.283
2.114AspPro: 2.114 ± 0.229
1.501AspGln: 1.501 ± 0.203
2.501AspArg: 2.501 ± 0.263
3.933AspSer: 3.933 ± 0.289
3.251AspThr: 3.251 ± 0.28
5.093AspVal: 5.093 ± 0.389
1.023AspTrp: 1.023 ± 0.166
3.183AspTyr: 3.183 ± 0.267
0.0AspXaa: 0.0 ± 0.0
Glu
5.525GluAla: 5.525 ± 0.367
0.5GluCys: 0.5 ± 0.125
6.252GluAsp: 6.252 ± 0.723
9.39GluGlu: 9.39 ± 0.653
3.729GluPhe: 3.729 ± 0.286
4.911GluGly: 4.911 ± 0.301
1.182GluHis: 1.182 ± 0.171
5.57GluIle: 5.57 ± 0.371
6.548GluLys: 6.548 ± 0.55
8.867GluLeu: 8.867 ± 0.563
2.183GluMet: 2.183 ± 0.205
3.592GluAsn: 3.592 ± 0.265
2.228GluPro: 2.228 ± 0.232
2.592GluGln: 2.592 ± 0.27
3.979GluArg: 3.979 ± 0.336
4.433GluSer: 4.433 ± 0.348
5.593GluThr: 5.593 ± 0.359
6.252GluVal: 6.252 ± 0.365
0.796GluTrp: 0.796 ± 0.139
3.501GluTyr: 3.501 ± 0.256
0.0GluXaa: 0.0 ± 0.0
Phe
1.842PheAla: 1.842 ± 0.252
0.477PheCys: 0.477 ± 0.104
3.115PheAsp: 3.115 ± 0.267
3.251PheGlu: 3.251 ± 0.288
1.591PhePhe: 1.591 ± 0.205
2.455PheGly: 2.455 ± 0.261
0.659PheHis: 0.659 ± 0.148
2.069PheIle: 2.069 ± 0.212
3.774PheLys: 3.774 ± 0.309
2.751PheLeu: 2.751 ± 0.246
0.932PheMet: 0.932 ± 0.136
2.501PheAsn: 2.501 ± 0.274
1.387PhePro: 1.387 ± 0.198
0.864PheGln: 0.864 ± 0.139
2.001PheArg: 2.001 ± 0.186
2.41PheSer: 2.41 ± 0.218
2.433PheThr: 2.433 ± 0.202
3.115PheVal: 3.115 ± 0.28
0.386PheTrp: 0.386 ± 0.1
1.523PheTyr: 1.523 ± 0.194
0.0PheXaa: 0.0 ± 0.0
Gly
3.751GlyAla: 3.751 ± 0.357
0.864GlyCys: 0.864 ± 0.16
4.547GlyAsp: 4.547 ± 0.331
4.843GlyGlu: 4.843 ± 0.281
3.115GlyPhe: 3.115 ± 0.246
4.092GlyGly: 4.092 ± 0.481
1.137GlyHis: 1.137 ± 0.18
3.751GlyIle: 3.751 ± 0.365
5.093GlyLys: 5.093 ± 0.374
4.706GlyLeu: 4.706 ± 0.318
1.591GlyMet: 1.591 ± 0.203
2.865GlyAsn: 2.865 ± 0.247
0.614GlyPro: 0.614 ± 0.131
1.955GlyGln: 1.955 ± 0.181
2.956GlyArg: 2.956 ± 0.298
3.888GlySer: 3.888 ± 0.338
3.842GlyThr: 3.842 ± 0.383
5.047GlyVal: 5.047 ± 0.373
1.023GlyTrp: 1.023 ± 0.16
2.637GlyTyr: 2.637 ± 0.259
0.0GlyXaa: 0.0 ± 0.0
His
0.978HisAla: 0.978 ± 0.162
0.273HisCys: 0.273 ± 0.082
0.978HisAsp: 0.978 ± 0.146
1.023HisGlu: 1.023 ± 0.14
0.546HisPhe: 0.546 ± 0.099
0.955HisGly: 0.955 ± 0.145
0.296HisHis: 0.296 ± 0.074
1.046HisIle: 1.046 ± 0.128
1.319HisLys: 1.319 ± 0.183
1.387HisLeu: 1.387 ± 0.188
0.341HisMet: 0.341 ± 0.095
0.75HisAsn: 0.75 ± 0.118
0.773HisPro: 0.773 ± 0.121
0.5HisGln: 0.5 ± 0.106
0.796HisArg: 0.796 ± 0.127
1.023HisSer: 1.023 ± 0.136
0.728HisThr: 0.728 ± 0.122
1.069HisVal: 1.069 ± 0.183
0.25HisTrp: 0.25 ± 0.06
0.818HisTyr: 0.818 ± 0.161
0.0HisXaa: 0.0 ± 0.0
Ile
3.478IleAla: 3.478 ± 0.29
0.296IleCys: 0.296 ± 0.088
4.638IleAsp: 4.638 ± 0.336
5.866IleGlu: 5.866 ± 0.394
2.023IlePhe: 2.023 ± 0.212
3.297IleGly: 3.297 ± 0.297
0.887IleHis: 0.887 ± 0.152
3.16IleIle: 3.16 ± 0.296
5.911IleLys: 5.911 ± 0.383
4.001IleLeu: 4.001 ± 0.29
1.569IleMet: 1.569 ± 0.2
3.274IleAsn: 3.274 ± 0.276
2.478IlePro: 2.478 ± 0.302
1.887IleGln: 1.887 ± 0.211
3.001IleArg: 3.001 ± 0.27
3.501IleSer: 3.501 ± 0.309
3.342IleThr: 3.342 ± 0.338
3.933IleVal: 3.933 ± 0.292
0.728IleTrp: 0.728 ± 0.14
2.069IleTyr: 2.069 ± 0.227
0.0IleXaa: 0.0 ± 0.0
Lys
5.411LysAla: 5.411 ± 0.458
0.75LysCys: 0.75 ± 0.151
5.365LysAsp: 5.365 ± 0.406
8.025LysGlu: 8.025 ± 0.517
3.16LysPhe: 3.16 ± 0.298
5.32LysGly: 5.32 ± 0.375
1.728LysHis: 1.728 ± 0.191
4.615LysIle: 4.615 ± 0.311
8.276LysLys: 8.276 ± 0.705
7.252LysLeu: 7.252 ± 0.454
2.251LysMet: 2.251 ± 0.24
3.842LysAsn: 3.842 ± 0.294
2.592LysPro: 2.592 ± 0.245
3.547LysGln: 3.547 ± 0.28
5.115LysArg: 5.115 ± 0.355
5.275LysSer: 5.275 ± 0.403
5.57LysThr: 5.57 ± 0.409
5.547LysVal: 5.547 ± 0.308
0.75LysTrp: 0.75 ± 0.154
2.978LysTyr: 2.978 ± 0.23
0.0LysXaa: 0.0 ± 0.0
Leu
5.161LeuAla: 5.161 ± 0.342
0.75LeuCys: 0.75 ± 0.137
6.275LeuAsp: 6.275 ± 0.425
7.821LeuGlu: 7.821 ± 0.443
2.91LeuPhe: 2.91 ± 0.305
4.752LeuGly: 4.752 ± 0.321
1.296LeuHis: 1.296 ± 0.167
4.592LeuIle: 4.592 ± 0.342
7.935LeuLys: 7.935 ± 0.533
6.98LeuLeu: 6.98 ± 0.447
1.978LeuMet: 1.978 ± 0.192
4.57LeuAsn: 4.57 ± 0.354
2.319LeuPro: 2.319 ± 0.226
3.365LeuGln: 3.365 ± 0.282
4.865LeuArg: 4.865 ± 0.321
5.525LeuSer: 5.525 ± 0.371
5.411LeuThr: 5.411 ± 0.375
5.547LeuVal: 5.547 ± 0.372
0.637LeuTrp: 0.637 ± 0.105
2.956LeuTyr: 2.956 ± 0.266
0.0LeuXaa: 0.0 ± 0.0
Met
1.546MetAla: 1.546 ± 0.193
0.205MetCys: 0.205 ± 0.075
0.955MetAsp: 0.955 ± 0.172
2.069MetGlu: 2.069 ± 0.209
0.909MetPhe: 0.909 ± 0.156
1.569MetGly: 1.569 ± 0.208
0.182MetHis: 0.182 ± 0.063
1.387MetIle: 1.387 ± 0.188
2.342MetLys: 2.342 ± 0.213
2.524MetLeu: 2.524 ± 0.216
0.568MetMet: 0.568 ± 0.126
1.546MetAsn: 1.546 ± 0.175
0.455MetPro: 0.455 ± 0.106
0.705MetGln: 0.705 ± 0.117
1.432MetArg: 1.432 ± 0.195
2.205MetSer: 2.205 ± 0.219
1.682MetThr: 1.682 ± 0.17
1.228MetVal: 1.228 ± 0.157
0.136MetTrp: 0.136 ± 0.055
0.887MetTyr: 0.887 ± 0.16
0.0MetXaa: 0.0 ± 0.0
Asn
2.887AsnAla: 2.887 ± 0.312
0.341AsnCys: 0.341 ± 0.079
2.728AsnAsp: 2.728 ± 0.257
3.638AsnGlu: 3.638 ± 0.317
1.91AsnPhe: 1.91 ± 0.215
3.842AsnGly: 3.842 ± 0.316
0.682AsnHis: 0.682 ± 0.122
2.728AsnIle: 2.728 ± 0.233
4.115AsnLys: 4.115 ± 0.248
3.956AsnLeu: 3.956 ± 0.335
1.319AsnMet: 1.319 ± 0.181
2.546AsnAsn: 2.546 ± 0.269
2.183AsnPro: 2.183 ± 0.224
1.159AsnGln: 1.159 ± 0.151
2.319AsnArg: 2.319 ± 0.23
3.092AsnSer: 3.092 ± 0.244
2.751AsnThr: 2.751 ± 0.303
3.842AsnVal: 3.842 ± 0.291
0.75AsnTrp: 0.75 ± 0.117
1.864AsnTyr: 1.864 ± 0.191
0.0AsnXaa: 0.0 ± 0.0
Pro
1.41ProAla: 1.41 ± 0.163
0.296ProCys: 0.296 ± 0.086
2.16ProAsp: 2.16 ± 0.211
3.137ProGlu: 3.137 ± 0.313
1.114ProPhe: 1.114 ± 0.158
1.637ProGly: 1.637 ± 0.195
0.568ProHis: 0.568 ± 0.127
2.16ProIle: 2.16 ± 0.218
2.615ProLys: 2.615 ± 0.237
2.501ProLeu: 2.501 ± 0.276
0.364ProMet: 0.364 ± 0.086
1.478ProAsn: 1.478 ± 0.153
0.978ProPro: 0.978 ± 0.145
0.796ProGln: 0.796 ± 0.129
1.364ProArg: 1.364 ± 0.182
2.205ProSer: 2.205 ± 0.224
2.069ProThr: 2.069 ± 0.238
2.183ProVal: 2.183 ± 0.259
0.386ProTrp: 0.386 ± 0.092
1.319ProTyr: 1.319 ± 0.171
0.0ProXaa: 0.0 ± 0.0
Gln
1.728GlnAla: 1.728 ± 0.192
0.136GlnCys: 0.136 ± 0.055
1.546GlnAsp: 1.546 ± 0.148
2.683GlnGlu: 2.683 ± 0.236
1.046GlnPhe: 1.046 ± 0.17
1.864GlnGly: 1.864 ± 0.249
0.637GlnHis: 0.637 ± 0.128
2.274GlnIle: 2.274 ± 0.218
2.592GlnLys: 2.592 ± 0.285
3.66GlnLeu: 3.66 ± 0.288
0.705GlnMet: 0.705 ± 0.142
1.296GlnAsn: 1.296 ± 0.158
1.091GlnPro: 1.091 ± 0.174
1.205GlnGln: 1.205 ± 0.18
1.682GlnArg: 1.682 ± 0.187
1.819GlnSer: 1.819 ± 0.163
2.001GlnThr: 2.001 ± 0.191
1.864GlnVal: 1.864 ± 0.244
0.341GlnTrp: 0.341 ± 0.109
1.069GlnTyr: 1.069 ± 0.143
0.0GlnXaa: 0.0 ± 0.0
Arg
2.865ArgAla: 2.865 ± 0.289
0.477ArgCys: 0.477 ± 0.136
3.183ArgAsp: 3.183 ± 0.283
3.751ArgGlu: 3.751 ± 0.308
1.955ArgPhe: 1.955 ± 0.218
2.956ArgGly: 2.956 ± 0.263
0.818ArgHis: 0.818 ± 0.124
2.887ArgIle: 2.887 ± 0.242
4.138ArgLys: 4.138 ± 0.287
4.388ArgLeu: 4.388 ± 0.275
1.432ArgMet: 1.432 ± 0.181
2.16ArgAsn: 2.16 ± 0.198
1.432ArgPro: 1.432 ± 0.167
1.842ArgGln: 1.842 ± 0.235
2.91ArgArg: 2.91 ± 0.28
2.455ArgSer: 2.455 ± 0.194
2.478ArgThr: 2.478 ± 0.274
4.32ArgVal: 4.32 ± 0.294
0.659ArgTrp: 0.659 ± 0.124
1.978ArgTyr: 1.978 ± 0.166
0.0ArgXaa: 0.0 ± 0.0
Ser
3.478SerAla: 3.478 ± 0.308
0.523SerCys: 0.523 ± 0.122
3.91SerAsp: 3.91 ± 0.347
4.934SerGlu: 4.934 ± 0.355
2.228SerPhe: 2.228 ± 0.263
3.888SerGly: 3.888 ± 0.267
0.841SerHis: 0.841 ± 0.156
3.956SerIle: 3.956 ± 0.305
5.456SerLys: 5.456 ± 0.404
5.525SerLeu: 5.525 ± 0.331
1.523SerMet: 1.523 ± 0.17
2.819SerAsn: 2.819 ± 0.265
1.955SerPro: 1.955 ± 0.203
2.137SerGln: 2.137 ± 0.217
2.546SerArg: 2.546 ± 0.237
3.797SerSer: 3.797 ± 0.307
3.41SerThr: 3.41 ± 0.368
4.456SerVal: 4.456 ± 0.282
0.932SerTrp: 0.932 ± 0.159
2.569SerTyr: 2.569 ± 0.264
0.0SerXaa: 0.0 ± 0.0
Thr
3.365ThrAla: 3.365 ± 0.361
0.5ThrCys: 0.5 ± 0.108
3.524ThrAsp: 3.524 ± 0.35
4.615ThrGlu: 4.615 ± 0.288
2.751ThrPhe: 2.751 ± 0.26
4.001ThrGly: 4.001 ± 0.345
1.046ThrHis: 1.046 ± 0.135
3.819ThrIle: 3.819 ± 0.293
5.115ThrLys: 5.115 ± 0.365
5.456ThrLeu: 5.456 ± 0.351
1.319ThrMet: 1.319 ± 0.192
2.478ThrAsn: 2.478 ± 0.206
2.569ThrPro: 2.569 ± 0.235
1.501ThrGln: 1.501 ± 0.182
3.001ThrArg: 3.001 ± 0.254
3.297ThrSer: 3.297 ± 0.268
3.729ThrThr: 3.729 ± 0.31
4.638ThrVal: 4.638 ± 0.312
0.75ThrTrp: 0.75 ± 0.115
2.501ThrTyr: 2.501 ± 0.244
0.0ThrXaa: 0.0 ± 0.0
Val
3.638ValAla: 3.638 ± 0.285
0.682ValCys: 0.682 ± 0.137
4.774ValAsp: 4.774 ± 0.276
6.616ValGlu: 6.616 ± 0.532
2.455ValPhe: 2.455 ± 0.292
4.297ValGly: 4.297 ± 0.34
1.205ValHis: 1.205 ± 0.171
4.229ValIle: 4.229 ± 0.347
6.252ValLys: 6.252 ± 0.358
5.547ValLeu: 5.547 ± 0.392
1.66ValMet: 1.66 ± 0.229
3.797ValAsn: 3.797 ± 0.286
2.546ValPro: 2.546 ± 0.263
2.16ValGln: 2.16 ± 0.213
3.206ValArg: 3.206 ± 0.268
4.797ValSer: 4.797 ± 0.366
4.911ValThr: 4.911 ± 0.303
6.184ValVal: 6.184 ± 0.451
0.909ValTrp: 0.909 ± 0.131
3.297ValTyr: 3.297 ± 0.289
0.0ValXaa: 0.0 ± 0.0
Trp
0.364TrpAla: 0.364 ± 0.091
0.091TrpCys: 0.091 ± 0.046
0.818TrpAsp: 0.818 ± 0.154
1.137TrpGlu: 1.137 ± 0.155
0.477TrpPhe: 0.477 ± 0.102
0.887TrpGly: 0.887 ± 0.153
0.091TrpHis: 0.091 ± 0.055
0.728TrpIle: 0.728 ± 0.117
1.069TrpLys: 1.069 ± 0.167
1.023TrpLeu: 1.023 ± 0.154
0.318TrpMet: 0.318 ± 0.077
0.659TrpAsn: 0.659 ± 0.125
0.159TrpPro: 0.159 ± 0.06
0.205TrpGln: 0.205 ± 0.063
0.477TrpArg: 0.477 ± 0.113
0.637TrpSer: 0.637 ± 0.116
0.659TrpThr: 0.659 ± 0.138
1.046TrpVal: 1.046 ± 0.149
0.159TrpTrp: 0.159 ± 0.062
0.682TrpTyr: 0.682 ± 0.11
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.251TyrAla: 2.251 ± 0.208
0.728TyrCys: 0.728 ± 0.145
2.524TyrAsp: 2.524 ± 0.239
3.001TyrGlu: 3.001 ± 0.269
1.432TyrPhe: 1.432 ± 0.175
2.705TyrGly: 2.705 ± 0.272
0.682TyrHis: 0.682 ± 0.134
2.364TyrIle: 2.364 ± 0.303
3.751TyrLys: 3.751 ± 0.271
3.046TyrLeu: 3.046 ± 0.291
0.864TyrMet: 0.864 ± 0.127
2.274TyrAsn: 2.274 ± 0.27
1.114TyrPro: 1.114 ± 0.154
1.546TyrGln: 1.546 ± 0.17
2.023TyrArg: 2.023 ± 0.217
2.637TyrSer: 2.637 ± 0.213
2.137TyrThr: 2.137 ± 0.224
3.069TyrVal: 3.069 ± 0.278
0.341TyrTrp: 0.341 ± 0.075
1.842TyrTyr: 1.842 ± 0.231
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 236 proteins (43986 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski