Raphanus sativus cryptic virus 2

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Duplopiviricetes; Durnavirales; Partitiviridae; unclassified Partitiviridae

Average proteome isoelectric point is 5.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q38L13|Q38L13_9VIRU Uncharacterized protein OS=Raphanus sativus cryptic virus 2 OX=351495 PE=4 SV=1
MM1 pKa = 7.73DD2 pKa = 4.61TAQTDD7 pKa = 3.26ADD9 pKa = 3.72KK10 pKa = 11.32AAGKK14 pKa = 10.15RR15 pKa = 11.84GATVPPEE22 pKa = 4.22GEE24 pKa = 3.91PAAKK28 pKa = 9.71TMRR31 pKa = 11.84FSDD34 pKa = 4.67AIVPAGPSKK43 pKa = 10.73DD44 pKa = 3.26ASTVATAALPKK55 pKa = 10.55DD56 pKa = 4.45LIQWPIEE63 pKa = 3.92RR64 pKa = 11.84LATQVNQVFLDD75 pKa = 3.62TTIQNGEE82 pKa = 3.98GGYY85 pKa = 9.54EE86 pKa = 4.06VPDD89 pKa = 3.96DD90 pKa = 4.16QIVPTLRR97 pKa = 11.84VYY99 pKa = 10.28EE100 pKa = 4.15INVTNLRR107 pKa = 11.84NAFRR111 pKa = 11.84SVLYY115 pKa = 9.89TILRR119 pKa = 11.84AKK121 pKa = 10.37FIEE124 pKa = 4.56RR125 pKa = 11.84GDD127 pKa = 3.84RR128 pKa = 11.84TVTAATTLATNVSNLTADD146 pKa = 3.13ACMAALYY153 pKa = 10.44AKK155 pKa = 10.12LRR157 pKa = 11.84SLHH160 pKa = 5.38RR161 pKa = 11.84QIYY164 pKa = 4.99THH166 pKa = 7.14KK167 pKa = 10.8GRR169 pKa = 11.84FTDD172 pKa = 3.41QPTYY176 pKa = 10.21TKK178 pKa = 10.5DD179 pKa = 3.3VEE181 pKa = 4.5LPLPFAVAIDD191 pKa = 4.49GIGMFRR197 pKa = 11.84TSAMSTRR204 pKa = 11.84FNVVPVYY211 pKa = 10.49PEE213 pKa = 3.59NTKK216 pKa = 10.89NEE218 pKa = 4.06GRR220 pKa = 11.84SHH222 pKa = 7.47DD223 pKa = 4.14SINFLEE229 pKa = 4.35YY230 pKa = 10.38KK231 pKa = 10.53SYY233 pKa = 10.87LAYY236 pKa = 10.02FAEE239 pKa = 4.49IGIPTRR245 pKa = 11.84TIDD248 pKa = 3.75TRR250 pKa = 11.84VTPGNAWWTYY260 pKa = 6.93RR261 pKa = 11.84TSYY264 pKa = 11.17DD265 pKa = 3.19GDD267 pKa = 3.87VYY269 pKa = 10.97DD270 pKa = 5.54LKK272 pKa = 11.24INFPPLHH279 pKa = 6.49FNDD282 pKa = 4.17HH283 pKa = 6.4LANLAIMFLGNSGADD298 pKa = 3.33RR299 pKa = 11.84NNASIITTKK308 pKa = 10.52TDD310 pKa = 3.18DD311 pKa = 3.92QDD313 pKa = 3.91YY314 pKa = 9.56GWYY317 pKa = 10.37VKK319 pKa = 10.25DD320 pKa = 3.34IRR322 pKa = 11.84EE323 pKa = 4.42GEE325 pKa = 4.08QARR328 pKa = 11.84AFAALCQWDD337 pKa = 3.63PRR339 pKa = 11.84YY340 pKa = 9.87WNEE343 pKa = 3.85KK344 pKa = 9.77NNVV347 pKa = 3.21

Molecular weight: 38.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q38L14|Q38L14_9VIRU Putative RNA dependent RNA polymerse OS=Raphanus sativus cryptic virus 2 OX=351495 PE=4 SV=1
MM1 pKa = 7.5AFEE4 pKa = 5.06SKK6 pKa = 9.47TSASEE11 pKa = 4.01STVNKK16 pKa = 9.88DD17 pKa = 3.04AAPAPSSASVKK28 pKa = 10.77SPVKK32 pKa = 10.01PSSSGLGRR40 pKa = 11.84AKK42 pKa = 10.07TEE44 pKa = 4.31TPHH47 pKa = 7.27LPASNYY53 pKa = 7.79EE54 pKa = 3.83IPRR57 pKa = 11.84YY58 pKa = 8.2TRR60 pKa = 11.84DD61 pKa = 3.38TQPVVGNKK69 pKa = 9.84NYY71 pKa = 10.17EE72 pKa = 3.66FSLRR76 pKa = 11.84MDD78 pKa = 3.2GRR80 pKa = 11.84LGHH83 pKa = 6.43SMDD86 pKa = 3.36RR87 pKa = 11.84SKK89 pKa = 9.87TARR92 pKa = 11.84PEE94 pKa = 4.27VNCSLNRR101 pKa = 11.84ANFHH105 pKa = 6.1EE106 pKa = 5.07GVLKK110 pKa = 10.34VLRR113 pKa = 11.84QILSEE118 pKa = 4.04MARR121 pKa = 11.84LKK123 pKa = 10.58QDD125 pKa = 3.37LSDD128 pKa = 4.67AEE130 pKa = 3.85IQEE133 pKa = 4.41RR134 pKa = 11.84VEE136 pKa = 4.56SVSLTLAYY144 pKa = 9.94GVATMAYY151 pKa = 9.68LKK153 pKa = 10.66LRR155 pKa = 11.84AINLFRR161 pKa = 11.84PNEE164 pKa = 3.8ASKK167 pKa = 10.72FLTKK171 pKa = 10.4PKK173 pKa = 10.54VPDD176 pKa = 3.32HH177 pKa = 6.73FEE179 pKa = 3.86IPTPFAFAISQLGVVEE195 pKa = 4.41VSSLSRR201 pKa = 11.84RR202 pKa = 11.84MICYY206 pKa = 7.61PTADD210 pKa = 4.1LADD213 pKa = 4.09ASNHH217 pKa = 5.19LVGNKK222 pKa = 9.82RR223 pKa = 11.84NWSQTAYY230 pKa = 10.68AEE232 pKa = 4.09AVRR235 pKa = 11.84YY236 pKa = 9.31AKK238 pKa = 10.55YY239 pKa = 10.71LGMSFSTVDD248 pKa = 3.7LDD250 pKa = 4.15IKK252 pKa = 11.02VGSSWWLFKK261 pKa = 10.72PDD263 pKa = 3.45VTDD266 pKa = 3.61GLLSIRR272 pKa = 11.84CPLPEE277 pKa = 5.58DD278 pKa = 3.91NYY280 pKa = 9.19TLAGATVFMLFYY292 pKa = 10.11HH293 pKa = 7.28DD294 pKa = 4.58VGSDD298 pKa = 3.4PAIDD302 pKa = 4.43LFNIDD307 pKa = 3.87SLGNDD312 pKa = 4.03DD313 pKa = 4.2YY314 pKa = 12.03GSFIRR319 pKa = 11.84NPRR322 pKa = 11.84DD323 pKa = 3.03GFNASSYY330 pKa = 10.17YY331 pKa = 10.64AISSEE336 pKa = 4.25GTDD339 pKa = 3.78EE340 pKa = 4.08MWKK343 pKa = 10.62SSAA346 pKa = 3.97

Molecular weight: 38.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1170

346

477

390.0

44.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.632 ± 1.116

0.769 ± 0.069

6.325 ± 0.496

5.556 ± 0.6

5.128 ± 0.675

5.043 ± 0.22

1.88 ± 0.219

5.556 ± 0.665

5.043 ± 0.274

8.034 ± 0.506

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.88 ± 0.256

4.701 ± 0.613

4.957 ± 0.474

3.162 ± 0.559

5.983 ± 0.128

7.863 ± 1.553

6.581 ± 1.162

6.496 ± 0.219

1.624 ± 0.165

4.786 ± 0.335
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski