Micromonospora craniellae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micromonosporales; Micromonosporaceae; Micromonospora

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5663 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A372FUU1|A0A372FUU1_9ACTN DUF1540 domain-containing protein OS=Micromonospora craniellae OX=2294034 GN=D0Q02_21710 PE=4 SV=1
MM1 pKa = 7.7TDD3 pKa = 4.1SPQQTYY9 pKa = 11.4DD10 pKa = 3.28DD11 pKa = 4.02AAAILSSALDD21 pKa = 3.83GNSDD25 pKa = 3.8GVVGTFDD32 pKa = 3.96AVVDD36 pKa = 3.93RR37 pKa = 11.84AGLVGAYY44 pKa = 9.73DD45 pKa = 3.97VAWCLAATMVGDD57 pKa = 4.16PVPPGACALEE67 pKa = 4.53FPDD70 pKa = 4.9IDD72 pKa = 3.64QAEE75 pKa = 4.14YY76 pKa = 10.35DD77 pKa = 4.02ARR79 pKa = 11.84WVARR83 pKa = 11.84FVSAYY88 pKa = 10.46ANSDD92 pKa = 3.35LDD94 pKa = 3.49TARR97 pKa = 11.84ALFGAAVADD106 pKa = 4.18GLLPEE111 pKa = 5.09CLLTLAGSTVATLRR125 pKa = 11.84HH126 pKa = 5.68RR127 pKa = 11.84AAA129 pKa = 4.48

Molecular weight: 13.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A372FR97|A0A372FR97_9ACTN Uncharacterized protein OS=Micromonospora craniellae OX=2294034 GN=D0Q02_29575 PE=4 SV=1
MM1 pKa = 7.13AQAAIRR7 pKa = 11.84KK8 pKa = 6.63NTGAGTAASSRR19 pKa = 11.84TAAKK23 pKa = 8.13RR24 pKa = 11.84TSPATTRR31 pKa = 11.84STGTAAARR39 pKa = 11.84KK40 pKa = 4.94TTATATGSRR49 pKa = 11.84AATSKK54 pKa = 10.75AAASTTGARR63 pKa = 11.84KK64 pKa = 6.33TTGSKK69 pKa = 9.28ATGSKK74 pKa = 10.38AATAKK79 pKa = 9.05ATSRR83 pKa = 11.84RR84 pKa = 11.84TTAGTAAATSARR96 pKa = 11.84TARR99 pKa = 11.84KK100 pKa = 9.13KK101 pKa = 10.59AVSATAGPTRR111 pKa = 11.84RR112 pKa = 11.84PTAKK116 pKa = 9.31ATAARR121 pKa = 11.84KK122 pKa = 7.92ATTAKK127 pKa = 9.35ATAAKK132 pKa = 9.6KK133 pKa = 10.56APAAKK138 pKa = 8.63KK139 pKa = 6.65TTVAKK144 pKa = 10.27KK145 pKa = 9.95AAAKK149 pKa = 9.28RR150 pKa = 11.84APASRR155 pKa = 11.84AAGGRR160 pKa = 11.84APATKK165 pKa = 9.34STAKK169 pKa = 10.25KK170 pKa = 9.5AATRR174 pKa = 11.84KK175 pKa = 8.09TSATQATAARR185 pKa = 11.84SRR187 pKa = 11.84AAGAPKK193 pKa = 10.04RR194 pKa = 11.84RR195 pKa = 11.84ATTMEE200 pKa = 4.02SFGSRR205 pKa = 11.84RR206 pKa = 11.84GARR209 pKa = 11.84TRR211 pKa = 3.3

Molecular weight: 20.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5663

0

5663

1872375

26

16823

330.6

35.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.607 ± 0.046

0.755 ± 0.012

6.198 ± 0.026

5.144 ± 0.032

2.613 ± 0.02

9.12 ± 0.031

2.259 ± 0.017

3.251 ± 0.025

1.559 ± 0.024

10.425 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.639 ± 0.013

1.763 ± 0.018

6.366 ± 0.037

2.852 ± 0.017

8.643 ± 0.039

4.808 ± 0.025

6.296 ± 0.029

9.088 ± 0.037

1.578 ± 0.014

2.038 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski