Papaver somniferum (Opium poppy)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; Ranunculales; Papaveraceae; Papaveroideae; Papaver

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 41351 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y7JSF3|A0A4Y7JSF3_PAPSO Lectin_legB domain-containing protein OS=Papaver somniferum OX=3469 GN=C5167_025387 PE=4 SV=1
MM1 pKa = 7.56APQYY5 pKa = 10.95KK6 pKa = 9.91GGCDD10 pKa = 3.24ACAGDD15 pKa = 5.67HH16 pKa = 6.89RR17 pKa = 11.84FADD20 pKa = 4.77CPWSQTPCINPKK32 pKa = 9.82CHH34 pKa = 5.46STKK37 pKa = 10.31ADD39 pKa = 3.43EE40 pKa = 4.66GVEE43 pKa = 4.08EE44 pKa = 4.34VDD46 pKa = 3.59GCVTFLLGAVEE57 pKa = 3.98EE58 pKa = 4.96DD59 pKa = 4.2GAVSLPFFWEE69 pKa = 3.69QAEE72 pKa = 4.25EE73 pKa = 4.17VDD75 pKa = 4.28GVVAAALPFDD85 pKa = 4.25NGGTSADD92 pKa = 3.92FFDD95 pKa = 5.59ADD97 pKa = 3.16VDD99 pKa = 4.17AVFGANKK106 pKa = 9.55VFLIFFVGAAVIQNSSPLTEE126 pKa = 4.51HH127 pKa = 7.32SILTPFFLGLDD138 pKa = 3.76PTGGPP143 pKa = 3.84

Molecular weight: 15.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y7K8L3|A0A4Y7K8L3_PAPSO ING domain-containing protein OS=Papaver somniferum OX=3469 GN=C5167_031912 PE=4 SV=1
MM1 pKa = 7.26RR2 pKa = 11.84RR3 pKa = 11.84VMVMMVMMVKK13 pKa = 10.33VLVVMMWMRR22 pKa = 11.84RR23 pKa = 11.84RR24 pKa = 11.84RR25 pKa = 11.84RR26 pKa = 11.84RR27 pKa = 11.84RR28 pKa = 11.84RR29 pKa = 11.84RR30 pKa = 11.84RR31 pKa = 11.84RR32 pKa = 11.84RR33 pKa = 11.84MRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84SSNNSRR44 pKa = 11.84LYY46 pKa = 10.3KK47 pKa = 9.51IRR49 pKa = 11.84RR50 pKa = 11.84RR51 pKa = 11.84SQVQMHH57 pKa = 6.36LVV59 pKa = 3.19

Molecular weight: 7.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

41351

0

41351

15615007

10

5096

377.6

42.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.326 ± 0.011

1.857 ± 0.006

5.326 ± 0.008

6.492 ± 0.014

4.221 ± 0.008

6.537 ± 0.012

2.319 ± 0.006

5.57 ± 0.01

6.379 ± 0.011

9.455 ± 0.014

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.516 ± 0.005

4.638 ± 0.008

4.681 ± 0.013

3.656 ± 0.01

5.084 ± 0.009

8.978 ± 0.015

5.142 ± 0.007

6.68 ± 0.009

1.263 ± 0.004

2.88 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski