Figwort mosaic virus (strain DxS) (FMV)
Average proteome isoelectric point is 7.59
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|P09523|POL_FMVD Enzymatic polyprotein OS=Figwort mosaic virus (strain DxS) OX=10650 GN=ORF V PE=3 SV=1
MM1 pKa = 7.69 AATLSEE7 pKa = 3.98 IRR9 pKa = 11.84 EE10 pKa = 4.57 LIQSLTKK17 pKa = 9.1 TANEE21 pKa = 3.63 IKK23 pKa = 10.86 AMLEE27 pKa = 3.87 RR28 pKa = 11.84 NSAGKK33 pKa = 7.97 PTGIEE38 pKa = 3.49 EE39 pKa = 3.99 AAAKK43 pKa = 9.74 IIKK46 pKa = 10.17 DD47 pKa = 3.19 IGDD50 pKa = 4.54 KK51 pKa = 10.12 IDD53 pKa = 3.25 QCEE56 pKa = 4.12 CTKK59 pKa = 10.66 KK60 pKa = 10.15 IEE62 pKa = 4.05 EE63 pKa = 4.46 MLDD66 pKa = 3.09 QKK68 pKa = 11.39 QNADD72 pKa = 3.68 TQIVPTKK79 pKa = 9.97 QEE81 pKa = 3.59 SSGLVKK87 pKa = 10.63 YY88 pKa = 10.31 SYY90 pKa = 10.68 PNWNVGNEE98 pKa = 4.11 EE99 pKa = 4.83 LGSSGNPNAVKK110 pKa = 10.03 WPPRR114 pKa = 11.84 KK115 pKa = 9.78
Molecular weight: 12.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.434
IPC2_protein 5.703
IPC_protein 5.499
Toseland 5.347
ProMoST 5.575
Dawson 5.423
Bjellqvist 5.55
Wikipedia 5.245
Rodwell 5.347
Grimsley 5.258
Solomon 5.41
Lehninger 5.372
Nozaki 5.525
DTASelect 5.601
Thurlkill 5.347
EMBOSS 5.258
Sillero 5.601
Patrickios 4.609
IPC_peptide 5.423
IPC2_peptide 5.588
IPC2.peptide.svr19 5.888
Protein with the highest isoelectric point:
>sp|P09522|VAP_FMVD Virion-associated protein OS=Figwort mosaic virus (strain DxS) OX=10650 GN=ORF III PE=3 SV=1
MM1 pKa = 7.45 SKK3 pKa = 10.6 DD4 pKa = 3.5 HH5 pKa = 7.39 LDD7 pKa = 3.72 FPHH10 pKa = 6.69 VYY12 pKa = 10.19 KK13 pKa = 10.75 KK14 pKa = 11.0 NKK16 pKa = 8.49 VLKK19 pKa = 10.36 LKK21 pKa = 10.49 PLDD24 pKa = 3.56 LSKK27 pKa = 11.15 NPRR30 pKa = 11.84 SYY32 pKa = 11.41 YY33 pKa = 10.29 FSSQNGSIQAIINHH47 pKa = 6.37 CNNINQITARR57 pKa = 11.84 NWLKK61 pKa = 10.52 LSKK64 pKa = 10.0 VLSYY68 pKa = 10.91 FGLEE72 pKa = 4.18 KK73 pKa = 10.33 DD74 pKa = 3.66 TSDD77 pKa = 4.95 SISKK81 pKa = 10.35 NKK83 pKa = 10.54 SPFNRR88 pKa = 11.84 FLKK91 pKa = 10.5 DD92 pKa = 2.76 ISHH95 pKa = 7.42 IFRR98 pKa = 11.84 EE99 pKa = 5.05 GEE101 pKa = 4.01 GSTKK105 pKa = 10.17 KK106 pKa = 10.58 ASEE109 pKa = 4.04 LGEE112 pKa = 3.76 ILEE115 pKa = 4.71 KK116 pKa = 10.61 IKK118 pKa = 11.12 NLDD121 pKa = 3.71 LKK123 pKa = 10.83 IEE125 pKa = 4.2 NLNKK129 pKa = 10.41 RR130 pKa = 11.84 IPDD133 pKa = 3.67 NLVTKK138 pKa = 10.62 ALIKK142 pKa = 10.56 EE143 pKa = 4.12 LVKK146 pKa = 11.18 DD147 pKa = 3.63 FDD149 pKa = 3.75 EE150 pKa = 5.18 RR151 pKa = 11.84 LTEE154 pKa = 3.96 VRR156 pKa = 11.84 DD157 pKa = 4.37 DD158 pKa = 3.39 IKK160 pKa = 11.25 KK161 pKa = 10.67 VIGG164 pKa = 3.57
Molecular weight: 18.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.782
IPC2_protein 8.843
IPC_protein 8.726
Toseland 9.823
ProMoST 9.341
Dawson 9.955
Bjellqvist 9.545
Wikipedia 10.058
Rodwell 10.657
Grimsley 9.984
Solomon 9.984
Lehninger 9.97
Nozaki 9.809
DTASelect 9.531
Thurlkill 9.823
EMBOSS 10.204
Sillero 9.867
Patrickios 10.35
IPC_peptide 9.984
IPC2_peptide 7.892
IPC2.peptide.svr19 7.803
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2335
66
666
333.6
38.4
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.582 ± 0.581
1.799 ± 0.325
5.139 ± 0.333
8.522 ± 1.197
4.582 ± 0.55
4.026 ± 0.29
1.842 ± 0.438
7.709 ± 0.389
11.649 ± 0.665
8.822 ± 0.773
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.713 ± 0.356
5.31 ± 0.58
3.597 ± 0.428
4.582 ± 0.527
3.94 ± 0.188
8.394 ± 0.613
4.968 ± 0.53
4.668 ± 0.704
1.156 ± 0.214
2.998 ± 0.232
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here