Floricoccus tropicus
Average proteome isoelectric point is 6.07
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2108 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1E8GNJ8|A0A1E8GNJ8_9LACT Uncharacterized protein OS=Floricoccus tropicus OX=1859473 GN=BG261_03065 PE=4 SV=1
MM1 pKa = 7.51 TEE3 pKa = 4.22 HH4 pKa = 6.46 NHH6 pKa = 6.11 DD7 pKa = 4.59 HH8 pKa = 5.86 NHH10 pKa = 7.01 DD11 pKa = 4.83 DD12 pKa = 3.75 IEE14 pKa = 4.68 LVTLYY19 pKa = 10.69 DD20 pKa = 3.73 EE21 pKa = 5.36 NGDD24 pKa = 3.65 EE25 pKa = 4.36 VLYY28 pKa = 10.42 EE29 pKa = 4.05 VYY31 pKa = 10.9 LEE33 pKa = 4.4 IEE35 pKa = 4.08 AQKK38 pKa = 10.96 EE39 pKa = 3.92 FGKK42 pKa = 10.4 DD43 pKa = 3.32 YY44 pKa = 11.02 ILLVPHH50 pKa = 7.23 DD51 pKa = 4.77 LNEE54 pKa = 4.63 DD55 pKa = 3.72 DD56 pKa = 4.84 EE57 pKa = 5.53 ALILPYY63 pKa = 10.44 AVEE66 pKa = 3.91 KK67 pKa = 11.06 DD68 pKa = 3.67 EE69 pKa = 4.52 NGEE72 pKa = 4.1 EE73 pKa = 4.05 IIVPISEE80 pKa = 5.19 DD81 pKa = 4.76 SDD83 pKa = 5.19 AEE85 pKa = 3.93 WNMIEE90 pKa = 4.45 EE91 pKa = 4.57 VFNSIVEE98 pKa = 4.24 EE99 pKa = 4.2 EE100 pKa = 4.09 QQ101 pKa = 3.1
Molecular weight: 11.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.741
IPC2_protein 3.732
IPC_protein 3.694
Toseland 3.503
ProMoST 3.821
Dawson 3.656
Bjellqvist 3.808
Wikipedia 3.554
Rodwell 3.528
Grimsley 3.414
Solomon 3.643
Lehninger 3.592
Nozaki 3.77
DTASelect 3.91
Thurlkill 3.541
EMBOSS 3.567
Sillero 3.808
Patrickios 0.947
IPC_peptide 3.643
IPC2_peptide 3.783
IPC2.peptide.svr19 3.718
Protein with the highest isoelectric point:
>tr|A0A1E8GLB8|A0A1E8GLB8_9LACT Uncharacterized protein OS=Floricoccus tropicus OX=1859473 GN=BG261_04760 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.29 QPSKK9 pKa = 10.26 RR10 pKa = 11.84 KK11 pKa = 8.87 RR12 pKa = 11.84 QKK14 pKa = 8.05 THH16 pKa = 5.1 GFRR19 pKa = 11.84 KK20 pKa = 10.02 RR21 pKa = 11.84 MSTKK25 pKa = 9.46 NGRR28 pKa = 11.84 RR29 pKa = 11.84 VLASRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 9.08 GRR39 pKa = 11.84 AVISAA44 pKa = 3.98
Molecular weight: 5.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.433
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.398
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.135
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.06
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2108
0
2108
628882
38
1747
298.3
33.58
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.511 ± 0.054
0.483 ± 0.013
6.241 ± 0.046
7.206 ± 0.062
4.765 ± 0.046
6.363 ± 0.047
1.485 ± 0.02
8.566 ± 0.059
7.74 ± 0.052
9.461 ± 0.062
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.558 ± 0.022
5.629 ± 0.048
3.1 ± 0.029
3.222 ± 0.032
3.485 ± 0.041
6.516 ± 0.059
5.246 ± 0.035
6.699 ± 0.042
0.813 ± 0.019
3.911 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here