Neodiprion lecontei (Redheaded pine sawfly)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Hymenoptera; Tenthredinoidea; Diprionidae; Diprioninae; Neodiprion<

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14458 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6J0BA94|A0A6J0BA94_NEOLC Actin-related protein 2 OS=Neodiprion lecontei OX=441921 GN=LOC107218490 PE=3 SV=1
MM1 pKa = 7.07VVLINFLLPQEE12 pKa = 4.61GVRR15 pKa = 11.84HH16 pKa = 5.74EE17 pKa = 4.61EE18 pKa = 3.92PDD20 pKa = 3.19TTLYY24 pKa = 10.78INDD27 pKa = 3.87RR28 pKa = 11.84EE29 pKa = 4.39VGKK32 pKa = 8.85GTLYY36 pKa = 9.55VTEE39 pKa = 5.16SLLSWVSGTTGQGFSLEE56 pKa = 4.14YY57 pKa = 10.49PNISLHH63 pKa = 6.77AISRR67 pKa = 11.84DD68 pKa = 3.8QQLHH72 pKa = 4.89PHH74 pKa = 5.78QYY76 pKa = 10.36LYY78 pKa = 11.9VMVDD82 pKa = 3.44AKK84 pKa = 10.93LDD86 pKa = 3.95PADD89 pKa = 3.87MQEE92 pKa = 4.87LLPIDD97 pKa = 4.12ARR99 pKa = 11.84SEE101 pKa = 4.3GNDD104 pKa = 3.55EE105 pKa = 4.68EE106 pKa = 6.82DD107 pKa = 5.56DD108 pKa = 4.29DD109 pKa = 6.07DD110 pKa = 5.09EE111 pKa = 6.77SEE113 pKa = 4.33TPMTEE118 pKa = 3.47MRR120 pKa = 11.84FVPEE124 pKa = 4.13NSNSLDD130 pKa = 3.4AMFQAMSQCQALHH143 pKa = 7.29PDD145 pKa = 3.59PQAEE149 pKa = 3.82EE150 pKa = 5.3DD151 pKa = 3.59IYY153 pKa = 11.5EE154 pKa = 4.41DD155 pKa = 5.16AEE157 pKa = 5.13DD158 pKa = 5.31DD159 pKa = 5.04VFKK162 pKa = 10.8CWEE165 pKa = 4.28AEE167 pKa = 3.9TDD169 pKa = 3.25AMPYY173 pKa = 10.17ILASDD178 pKa = 3.51EE179 pKa = 4.89AYY181 pKa = 10.43NQNGADD187 pKa = 3.66TDD189 pKa = 3.53EE190 pKa = 5.51AMDD193 pKa = 3.59IEE195 pKa = 4.82AGQFEE200 pKa = 4.86DD201 pKa = 5.63AEE203 pKa = 4.32EE204 pKa = 4.4DD205 pKa = 3.54PP206 pKa = 5.23

Molecular weight: 23.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6J0BWA8|A0A6J0BWA8_NEOLC ATP synthase subunit delta mitochondrial OS=Neodiprion lecontei OX=441921 GN=LOC107223444 PE=3 SV=1
MM1 pKa = 7.44SAHH4 pKa = 6.25KK5 pKa = 9.51TFIVKK10 pKa = 10.33RR11 pKa = 11.84KK12 pKa = 8.73LAKK15 pKa = 9.93KK16 pKa = 10.12LKK18 pKa = 8.64QNRR21 pKa = 11.84PIPQWVRR28 pKa = 11.84MRR30 pKa = 11.84TGNTIRR36 pKa = 11.84YY37 pKa = 5.79NAKK40 pKa = 8.47RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.1WRR45 pKa = 11.84RR46 pKa = 11.84TKK48 pKa = 10.89LKK50 pKa = 10.55LL51 pKa = 3.39

Molecular weight: 6.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11567

2891

14458

9009225

34

18177

623.1

69.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.623 ± 0.021

1.824 ± 0.03

5.546 ± 0.021

6.83 ± 0.028

3.433 ± 0.015

6.02 ± 0.028

2.457 ± 0.012

5.277 ± 0.017

6.108 ± 0.04

8.955 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.176 ± 0.012

4.765 ± 0.017

5.428 ± 0.025

4.327 ± 0.022

5.633 ± 0.02

8.244 ± 0.028

5.994 ± 0.017

6.331 ± 0.017

1.093 ± 0.007

2.918 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski