Xylella phage Prado

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Pradovirus; Xylella virus Prado

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V5Q7T3|V5Q7T3_9CAUD Uncharacterized protein OS=Xylella phage Prado OX=1415146 GN=Prado_05 PE=4 SV=1
MM1 pKa = 7.62AVPDD5 pKa = 4.03PQLRR9 pKa = 11.84YY10 pKa = 8.97ATDD13 pKa = 3.2VFDD16 pKa = 5.4ANGVTTDD23 pKa = 3.2WQISFTGGYY32 pKa = 8.94IDD34 pKa = 4.74PSHH37 pKa = 7.19VYY39 pKa = 10.7AMSGILDD46 pKa = 4.18EE47 pKa = 4.42EE48 pKa = 4.69TQLLTDD54 pKa = 4.03RR55 pKa = 11.84TSHH58 pKa = 4.5TVEE61 pKa = 4.01VLSEE65 pKa = 4.45DD66 pKa = 4.13EE67 pKa = 4.13NSSTVRR73 pKa = 11.84VEE75 pKa = 3.74PAVAAGRR82 pKa = 11.84KK83 pKa = 9.05LYY85 pKa = 9.94IYY87 pKa = 10.25RR88 pKa = 11.84STPVQQMLVDD98 pKa = 3.85YY99 pKa = 10.22VNGSIISKK107 pKa = 9.44TNLNLSNDD115 pKa = 3.58QLLKK119 pKa = 10.39IIQEE123 pKa = 4.34MFDD126 pKa = 3.67SLNIATLSIDD136 pKa = 3.36QQVGVVVDD144 pKa = 4.16LNKK147 pKa = 10.29IITEE151 pKa = 4.02IYY153 pKa = 10.49KK154 pKa = 10.54EE155 pKa = 4.09VLEE158 pKa = 4.35LLAAGGIVSVAPRR171 pKa = 11.84VWSGSWTGDD180 pKa = 3.32QADD183 pKa = 4.09DD184 pKa = 3.86TDD186 pKa = 4.61FDD188 pKa = 4.09MVGADD193 pKa = 3.27VSGAGFYY200 pKa = 10.67DD201 pKa = 4.08VYY203 pKa = 11.61VNGIGMQPDD212 pKa = 2.79VDD214 pKa = 4.18YY215 pKa = 11.27QVTLADD221 pKa = 3.63GTDD224 pKa = 3.34PSFIRR229 pKa = 11.84FATVPAEE236 pKa = 3.82GSIWFAVLRR245 pKa = 11.84GYY247 pKa = 10.59AKK249 pKa = 10.11PYY251 pKa = 9.02TGPAPITATSLRR263 pKa = 11.84VPIIPAEE270 pKa = 4.31GPTYY274 pKa = 10.52YY275 pKa = 10.68ADD277 pKa = 3.52KK278 pKa = 9.47ATEE281 pKa = 4.03YY282 pKa = 11.28GLVRR286 pKa = 11.84CTYY289 pKa = 11.11AGGCTVNINLIPEE302 pKa = 4.32VGDD305 pKa = 3.95GEE307 pKa = 4.63TKK309 pKa = 10.39LASGSYY315 pKa = 10.34FSVQQKK321 pKa = 10.22AGPVVITGDD330 pKa = 3.16AGVTLEE336 pKa = 4.57VPAGCIAATRR346 pKa = 11.84GTNSVVTATCIDD358 pKa = 3.65GDD360 pKa = 4.23TNTWLLSGDD369 pKa = 3.9LAKK372 pKa = 10.54EE373 pKa = 3.9

Molecular weight: 39.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V5Q7T5|V5Q7T5_9CAUD Terminase small subunit OS=Xylella phage Prado OX=1415146 GN=Prado_46 PE=4 SV=1
MM1 pKa = 7.38IPVIFKK7 pKa = 10.2RR8 pKa = 11.84RR9 pKa = 11.84HH10 pKa = 4.76NGKK13 pKa = 9.86RR14 pKa = 11.84SPMQLAAQPRR24 pKa = 11.84QWKK27 pKa = 9.68EE28 pKa = 3.33FVTYY32 pKa = 9.13EE33 pKa = 3.9HH34 pKa = 6.96SVEE37 pKa = 3.49RR38 pKa = 11.84WYY40 pKa = 11.0AA41 pKa = 3.13

Molecular weight: 5.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

13776

41

1632

264.9

29.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.816 ± 0.674

0.777 ± 0.141

6.127 ± 0.194

6.214 ± 0.287

3.201 ± 0.178

8.094 ± 0.367

1.786 ± 0.21

4.443 ± 0.162

4.936 ± 0.385

8.21 ± 0.231

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.584 ± 0.179

4.225 ± 0.171

4.305 ± 0.3

4.305 ± 0.264

6.315 ± 0.338

5.771 ± 0.289

5.873 ± 0.251

6.976 ± 0.26

1.67 ± 0.11

3.375 ± 0.174
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski