Thioploca ingrica
Average proteome isoelectric point is 6.38
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3951 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A090AIY7|A0A090AIY7_9GAMM Uncharacterized protein OS=Thioploca ingrica OX=40754 GN=THII_1155 PE=4 SV=1
MM1 pKa = 7.48 KK2 pKa = 10.46 FSDD5 pKa = 4.05 EE6 pKa = 4.2 DD7 pKa = 3.95 VEE9 pKa = 4.91 LLAEE13 pKa = 4.3 LFEE16 pKa = 4.31 EE17 pKa = 4.4 QQYY20 pKa = 10.91 QDD22 pKa = 3.8 EE23 pKa = 4.84 LMLGLQTIDD32 pKa = 2.7 SHH34 pKa = 6.11 MLVPSNQDD42 pKa = 2.87 WHH44 pKa = 7.06 PDD46 pKa = 2.99 FAEE49 pKa = 4.72 IYY51 pKa = 8.59 NQLIEE56 pKa = 5.23 LEE58 pKa = 5.04 DD59 pKa = 3.99 GLPYY63 pKa = 9.95 IPYY66 pKa = 9.88 ISLVKK71 pKa = 10.35 HH72 pKa = 6.01
Molecular weight: 8.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.767
IPC2_protein 3.91
IPC_protein 3.808
Toseland 3.63
ProMoST 3.961
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.706
Rodwell 3.656
Grimsley 3.554
Solomon 3.77
Lehninger 3.719
Nozaki 3.923
DTASelect 4.062
Thurlkill 3.681
EMBOSS 3.719
Sillero 3.935
Patrickios 0.769
IPC_peptide 3.77
IPC2_peptide 3.91
IPC2.peptide.svr19 3.839
Protein with the highest isoelectric point:
>tr|A0A090AJM4|A0A090AJM4_9GAMM Polyhydroxyalkonate synthesis repressor PhaR OS=Thioploca ingrica OX=40754 GN=THII_0838 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.53 RR3 pKa = 11.84 TFQPSILVRR12 pKa = 11.84 KK13 pKa = 8.93 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.49 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MKK23 pKa = 9.75 TKK25 pKa = 10.43 DD26 pKa = 3.02 GRR28 pKa = 11.84 KK29 pKa = 8.8 IIKK32 pKa = 9.64 ARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.3 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LSAA44 pKa = 3.91
Molecular weight: 5.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.426
IPC2_protein 10.921
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.398
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.135
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.077
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3951
0
3951
1382316
39
6326
349.9
39.16
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.839 ± 0.043
1.036 ± 0.016
4.748 ± 0.032
6.067 ± 0.044
3.88 ± 0.024
6.334 ± 0.047
2.37 ± 0.021
7.027 ± 0.034
4.826 ± 0.037
11.176 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.041 ± 0.022
4.493 ± 0.036
4.775 ± 0.031
5.748 ± 0.043
4.584 ± 0.038
5.83 ± 0.032
6.293 ± 0.049
6.237 ± 0.034
1.448 ± 0.02
3.248 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here