Avian metaavulavirus 2
Average proteome isoelectric point is 6.29
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q916F8|Q916F8_9MONO Hemagglutinin-neuraminidase OS=Avian metaavulavirus 2 OX=2560313 GN=HN PE=3 SV=1
MM1 pKa = 6.87 NQALVILLVSFQLGVALDD19 pKa = 3.93 NSVLAPIGVASAQEE33 pKa = 4.0 WQLAAYY39 pKa = 6.49 TTTLTGTIAVRR50 pKa = 11.84 FIPVLPGNLSTCAQEE65 pKa = 4.48 TLQEE69 pKa = 4.11 YY70 pKa = 10.47 NRR72 pKa = 11.84 TVTNILGPLRR82 pKa = 11.84 EE83 pKa = 4.36 NLDD86 pKa = 3.68 ALLSDD91 pKa = 4.23 FDD93 pKa = 4.25 KK94 pKa = 10.78 PASRR98 pKa = 11.84 FVGAIIGSVALGVATAAQITAAVALNQAQEE128 pKa = 3.82 NARR131 pKa = 11.84 NIWRR135 pKa = 11.84 LKK137 pKa = 10.27 EE138 pKa = 4.31 SIKK141 pKa = 9.74 KK142 pKa = 8.26 TNEE145 pKa = 3.36 AVLEE149 pKa = 4.42 LKK151 pKa = 10.82 DD152 pKa = 4.01 GLATTAIALDD162 pKa = 3.89 KK163 pKa = 10.27 VQKK166 pKa = 10.5 FINDD170 pKa = 4.08 DD171 pKa = 4.22 IIPQIKK177 pKa = 10.48 DD178 pKa = 2.7 IDD180 pKa = 4.17 CQVVANKK187 pKa = 10.52 LGVYY191 pKa = 10.44 LSLYY195 pKa = 8.74 LTEE198 pKa = 4.3 LTTVFGSQITNPALSTLSYY217 pKa = 9.72 QALYY221 pKa = 9.92 SLCGGDD227 pKa = 3.27 MGKK230 pKa = 8.33 LTEE233 pKa = 5.08 LIGVNAKK240 pKa = 10.31 DD241 pKa = 3.4 VGSLYY246 pKa = 10.33 EE247 pKa = 4.26 ANLITGQIVGYY258 pKa = 10.32 DD259 pKa = 3.66 PEE261 pKa = 4.29 LQIILIQVSYY271 pKa = 10.28 PSVSEE276 pKa = 4.1 VTGVRR281 pKa = 11.84 ATEE284 pKa = 4.01 LVTVSVTTPKK294 pKa = 11.1 GEE296 pKa = 4.01 GQAIVPRR303 pKa = 11.84 YY304 pKa = 7.88 VAQSRR309 pKa = 11.84 VLTEE313 pKa = 3.93 EE314 pKa = 4.94 LDD316 pKa = 3.61 VSTCRR321 pKa = 11.84 FSKK324 pKa = 7.66 TTLYY328 pKa = 10.42 CRR330 pKa = 11.84 SILTRR335 pKa = 11.84 PLPTLIASCLSGKK348 pKa = 10.3 YY349 pKa = 9.82 DD350 pKa = 3.52 DD351 pKa = 4.82 CQYY354 pKa = 8.24 TTEE357 pKa = 4.23 IGALSSRR364 pKa = 11.84 FITVNGGVLANCRR377 pKa = 11.84 AIVCKK382 pKa = 10.23 CVSPPHH388 pKa = 7.16 IIPQNDD394 pKa = 3.17 IGSVTVIDD402 pKa = 3.88 SSICKK407 pKa = 9.49 EE408 pKa = 4.06 VVLEE412 pKa = 4.16 SVQLRR417 pKa = 11.84 LEE419 pKa = 4.66 GKK421 pKa = 10.18 LSSQYY426 pKa = 10.48 FSNVTIDD433 pKa = 3.86 LSQITTSGSLDD444 pKa = 3.1 ISSEE448 pKa = 3.63 IGSINNTVNRR458 pKa = 11.84 VDD460 pKa = 3.94 EE461 pKa = 4.94 LIKK464 pKa = 10.72 EE465 pKa = 4.33 SNEE468 pKa = 3.23 WLNAVNPRR476 pKa = 11.84 LVNNTSIIVLCVLAALIIVWLIALTVCFCYY506 pKa = 10.1 SARR509 pKa = 11.84 YY510 pKa = 8.12 SAKK513 pKa = 9.39 SKK515 pKa = 9.3 QMRR518 pKa = 11.84 GAMTGIDD525 pKa = 3.9 NPYY528 pKa = 9.76 VIQSATKK535 pKa = 8.27 MM536 pKa = 3.57
Molecular weight: 57.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.943
IPC2_protein 5.13
IPC_protein 5.029
Toseland 4.889
ProMoST 5.13
Dawson 4.978
Bjellqvist 5.118
Wikipedia 4.825
Rodwell 4.876
Grimsley 4.8
Solomon 4.978
Lehninger 4.927
Nozaki 5.08
DTASelect 5.232
Thurlkill 4.889
EMBOSS 4.851
Sillero 5.143
Patrickios 3.617
IPC_peptide 4.978
IPC2_peptide 5.143
IPC2.peptide.svr19 5.155
Protein with the highest isoelectric point:
>tr|F5BH21|F5BH21_9MONO Nucleocapsid OS=Avian metaavulavirus 2 OX=2560313 GN=NP PE=3 SV=1
MM1 pKa = 7.29 AQTTVRR7 pKa = 11.84 LYY9 pKa = 10.34 IDD11 pKa = 3.69 EE12 pKa = 4.89 ASPDD16 pKa = 3.84 IEE18 pKa = 4.26 LLSYY22 pKa = 9.69 PLIMKK27 pKa = 7.92 DD28 pKa = 3.01 TGHH31 pKa = 5.99 GTKK34 pKa = 10.07 EE35 pKa = 3.77 LQQQIRR41 pKa = 11.84 VAEE44 pKa = 4.21 IGALQGGKK52 pKa = 9.74 NEE54 pKa = 4.47 SVFINAYY61 pKa = 10.42 GFVQQCKK68 pKa = 8.58 VKK70 pKa = 10.53 PGATQFFQVDD80 pKa = 3.38 AATKK84 pKa = 9.76 PEE86 pKa = 4.3 VVTAGMIIIGAVKK99 pKa = 9.95 GVAGITKK106 pKa = 9.77 LAEE109 pKa = 4.0 EE110 pKa = 4.45 VFEE113 pKa = 5.03 LDD115 pKa = 3.82 ISIKK119 pKa = 10.38 KK120 pKa = 9.64 SASFHH125 pKa = 5.93 EE126 pKa = 4.62 KK127 pKa = 10.09 VAVSFNTVPLSLMNSTACRR146 pKa = 11.84 NLGYY150 pKa = 8.83 VTNAEE155 pKa = 4.15 EE156 pKa = 5.34 AIKK159 pKa = 10.62 CPSKK163 pKa = 10.24 IQAGVTYY170 pKa = 9.58 KK171 pKa = 10.41 FKK173 pKa = 11.01 IMFVSLTRR181 pKa = 11.84 LHH183 pKa = 6.25 NGKK186 pKa = 10.26 LYY188 pKa = 10.2 RR189 pKa = 11.84 VPKK192 pKa = 10.19 AVYY195 pKa = 9.61 AVEE198 pKa = 4.78 ASALYY203 pKa = 9.93 KK204 pKa = 10.58 VQLEE208 pKa = 4.43 VGFKK212 pKa = 10.63 LDD214 pKa = 3.26 VAKK217 pKa = 10.71 DD218 pKa = 3.66 HH219 pKa = 6.72 PHH221 pKa = 6.0 VKK223 pKa = 9.16 MLKK226 pKa = 8.55 KK227 pKa = 10.33 VEE229 pKa = 4.37 RR230 pKa = 11.84 NGEE233 pKa = 4.16 TLYY236 pKa = 11.14 LGYY239 pKa = 11.09 AWFHH243 pKa = 6.03 LCNFKK248 pKa = 9.24 KK249 pKa = 9.31 TNAKK253 pKa = 10.21 GEE255 pKa = 4.16 SRR257 pKa = 11.84 TISNLEE263 pKa = 3.82 GKK265 pKa = 9.05 VRR267 pKa = 11.84 AMGIKK272 pKa = 10.16 VSLYY276 pKa = 10.44 DD277 pKa = 3.23 LWGPTLVVQITGKK290 pKa = 7.51 TSKK293 pKa = 10.03 YY294 pKa = 9.15 AQGFFSTTGTCCLPVSKK311 pKa = 10.42 AAPEE315 pKa = 3.93 LAKK318 pKa = 11.1 LMWSCNATIVEE329 pKa = 4.28 AAVIIQGSDD338 pKa = 2.52 RR339 pKa = 11.84 RR340 pKa = 11.84 AVVTSEE346 pKa = 3.67 DD347 pKa = 3.89 LEE349 pKa = 4.82 VYY351 pKa = 10.41 GAVAKK356 pKa = 9.89 EE357 pKa = 3.67 KK358 pKa = 9.91 QAAKK362 pKa = 10.18 GFHH365 pKa = 7.1 PFRR368 pKa = 11.84 KK369 pKa = 9.78
Molecular weight: 40.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.736
IPC2_protein 8.624
IPC_protein 8.478
Toseland 9.268
ProMoST 9.092
Dawson 9.545
Bjellqvist 9.311
Wikipedia 9.677
Rodwell 9.882
Grimsley 9.589
Solomon 9.575
Lehninger 9.545
Nozaki 9.487
DTASelect 9.238
Thurlkill 9.414
EMBOSS 9.706
Sillero 9.531
Patrickios 4.863
IPC_peptide 9.575
IPC2_peptide 7.907
IPC2.peptide.svr19 7.826
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
4583
369
2242
763.8
84.54
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.008 ± 0.937
1.898 ± 0.271
5.062 ± 0.419
5.062 ± 0.374
3.491 ± 0.461
5.651 ± 0.646
1.898 ± 0.412
7.004 ± 0.679
4.931 ± 0.792
10.168 ± 0.827
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.335 ± 0.378
4.32 ± 0.408
4.08 ± 0.385
4.757 ± 0.277
5.04 ± 0.511
8.662 ± 0.463
6.655 ± 0.479
6.917 ± 0.626
1.069 ± 0.131
2.989 ± 0.268
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here