Pseudooctadecabacter jejudonensis
Average proteome isoelectric point is 5.95
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3340 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Y5SQ16|A0A1Y5SQ16_9RHOB Acetolactate synthase OS=Pseudooctadecabacter jejudonensis OX=1391910 GN=alsS PE=3 SV=1
MM1 pKa = 7.5 IRR3 pKa = 11.84 LLPLFALLPHH13 pKa = 7.06 AAHH16 pKa = 7.25 ADD18 pKa = 3.57 WAPRR22 pKa = 11.84 PAMFDD27 pKa = 3.39 YY28 pKa = 11.11 SSAFAVCTAQPDD40 pKa = 3.85 ARR42 pKa = 11.84 DD43 pKa = 3.63 LATACADD50 pKa = 3.79 TLEE53 pKa = 4.22 AAYY56 pKa = 9.67 ILKK59 pKa = 10.4 RR60 pKa = 11.84 AVAQAAFVCADD71 pKa = 4.09 TPLSGCPVPLEE82 pKa = 4.72 DD83 pKa = 4.8 EE84 pKa = 4.47 GLPAIAARR92 pKa = 11.84 IAGDD96 pKa = 3.8 IGCDD100 pKa = 3.24 STPIEE105 pKa = 4.33 TLPTDD110 pKa = 3.33 TALPRR115 pKa = 11.84 DD116 pKa = 3.64 HH117 pKa = 7.15 CVALTADD124 pKa = 3.09 IMFDD128 pKa = 3.37 EE129 pKa = 5.13 GVVPLFTDD137 pKa = 4.62 LSCDD141 pKa = 3.81 GLPSEE146 pKa = 5.47 CDD148 pKa = 3.36 DD149 pKa = 5.09 LADD152 pKa = 3.17 IHH154 pKa = 6.65 AALWVQAVDD163 pKa = 5.04 ALTHH167 pKa = 7.17 DD168 pKa = 5.08 DD169 pKa = 3.79 PTITDD174 pKa = 4.02 LQARR178 pKa = 11.84 NLNTCTTQDD187 pKa = 3.65 DD188 pKa = 3.73 ARR190 pKa = 11.84 ACIAARR196 pKa = 11.84 AAEE199 pKa = 4.23 LWVDD203 pKa = 5.11 LVGQDD208 pKa = 3.83 PLL210 pKa = 4.16
Molecular weight: 22.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.705
IPC2_protein 3.872
IPC_protein 3.897
Toseland 3.656
ProMoST 4.075
Dawson 3.91
Bjellqvist 4.062
Wikipedia 3.872
Rodwell 3.719
Grimsley 3.567
Solomon 3.897
Lehninger 3.859
Nozaki 4.024
DTASelect 4.317
Thurlkill 3.732
EMBOSS 3.884
Sillero 4.024
Patrickios 1.189
IPC_peptide 3.897
IPC2_peptide 3.999
IPC2.peptide.svr19 3.896
Protein with the highest isoelectric point:
>tr|A0A1Y5RYE7|A0A1Y5RYE7_9RHOB Uncharacterized protein OS=Pseudooctadecabacter jejudonensis OX=1391910 GN=PSJ8397_01108 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNRR10 pKa = 11.84 VRR12 pKa = 11.84 KK13 pKa = 8.99 NRR15 pKa = 11.84 HH16 pKa = 3.77 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 AGRR28 pKa = 11.84 KK29 pKa = 8.54 ILNARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.93 GRR39 pKa = 11.84 KK40 pKa = 8.21 EE41 pKa = 3.63 LSAA44 pKa = 4.99
Molecular weight: 5.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.403
IPC2_protein 10.862
IPC_protein 12.442
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.34
Grimsley 12.647
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.076
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.067
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3340
0
3340
1035149
30
4019
309.9
33.58
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.267 ± 0.061
0.895 ± 0.015
6.747 ± 0.046
5.408 ± 0.036
3.81 ± 0.028
8.605 ± 0.049
2.062 ± 0.022
5.249 ± 0.028
3.143 ± 0.035
9.572 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.896 ± 0.022
2.745 ± 0.027
4.949 ± 0.031
3.313 ± 0.024
6.171 ± 0.043
4.972 ± 0.028
6.107 ± 0.049
7.471 ± 0.038
1.393 ± 0.02
2.225 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here