Neptunomonas qingdaonensis
Average proteome isoelectric point is 6.12
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4011 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1I2LMB4|A0A1I2LMB4_9GAMM Simple sugar transport system substrate-binding protein OS=Neptunomonas qingdaonensis OX=1045558 GN=SAMN05216175_10138 PE=3 SV=1
NN1 pKa = 7.77 GIQDD5 pKa = 3.53 TGEE8 pKa = 4.26 GGVAGAKK15 pKa = 9.95 VSLFNCDD22 pKa = 2.22 GTAANDD28 pKa = 3.48 INGNPVASITTGGDD42 pKa = 3.27 GIYY45 pKa = 10.72 NFTNLAPGEE54 pKa = 4.35 YY55 pKa = 8.92 YY56 pKa = 10.98 VQFTAPSGYY65 pKa = 10.88 VITQQLATEE74 pKa = 4.6 ADD76 pKa = 3.55 AAGDD80 pKa = 4.0 DD81 pKa = 4.1 SDD83 pKa = 5.71 ANAAGITSCVVLEE96 pKa = 4.26 SGEE99 pKa = 4.17 TEE101 pKa = 3.98 EE102 pKa = 5.73 RR103 pKa = 11.84 VDD105 pKa = 5.0 AGLVLQNAGIDD116 pKa = 3.19 IEE118 pKa = 4.63 KK119 pKa = 10.01 YY120 pKa = 9.35 VRR122 pKa = 11.84 GRR124 pKa = 11.84 YY125 pKa = 8.81 EE126 pKa = 4.17 GEE128 pKa = 4.02 SGTDD132 pKa = 3.05 EE133 pKa = 4.49 GLTPGFWKK141 pKa = 8.45 THH143 pKa = 5.13 SEE145 pKa = 4.16 FGPAPLAGWPEE156 pKa = 4.35 SGFDD160 pKa = 3.48 PTDD163 pKa = 3.34 SYY165 pKa = 12.1 EE166 pKa = 4.78 SIFSVEE172 pKa = 4.28 VPGTPSLLDD181 pKa = 3.66 ALNSGGGDD189 pKa = 3.29 LDD191 pKa = 3.43 ALMRR195 pKa = 11.84 HH196 pKa = 5.28 STAALLNASNPFVAYY211 pKa = 9.2 EE212 pKa = 3.73 YY213 pKa = 10.77 SQAEE217 pKa = 4.88 VISMTQAAISSGVYY231 pKa = 10.21 EE232 pKa = 3.89 ITKK235 pKa = 10.62 DD236 pKa = 3.18 LFAAKK241 pKa = 10.26 NEE243 pKa = 4.18 LGADD247 pKa = 3.79 LNEE250 pKa = 4.5 GTSEE254 pKa = 4.03 GTIVEE259 pKa = 4.73 TIDD262 pKa = 3.57 YY263 pKa = 10.68 DD264 pKa = 4.26 ADD266 pKa = 4.13 TPDD269 pKa = 3.88 GPYY272 pKa = 10.13 IPIDD276 pKa = 3.45 GEE278 pKa = 4.5 AIFTYY283 pKa = 10.63 VVTNTGDD290 pKa = 3.22 VALSDD295 pKa = 3.78 VQVTDD300 pKa = 4.13 DD301 pKa = 3.47 RR302 pKa = 11.84 LANVTLVGGDD312 pKa = 3.68 TNNDD316 pKa = 2.95 NLLDD320 pKa = 3.92 VGEE323 pKa = 4.38 TWTYY327 pKa = 7.95 TASEE331 pKa = 4.37 TVVAGGTFVNTGTVTGADD349 pKa = 3.01 ATGRR353 pKa = 11.84 ILSDD357 pKa = 3.4 SDD359 pKa = 3.55 LAHH362 pKa = 6.67 YY363 pKa = 7.51 NTSFLTSSLGDD374 pKa = 3.78 RR375 pKa = 11.84 VWLDD379 pKa = 3.56 LDD381 pKa = 3.91 SDD383 pKa = 4.93 GIQDD387 pKa = 3.48 NDD389 pKa = 3.32 EE390 pKa = 4.53 AGVAGVTVNLLDD402 pKa = 3.69 SDD404 pKa = 4.66 GNVSATTITDD414 pKa = 3.26 DD415 pKa = 3.37 YY416 pKa = 11.58 GNYY419 pKa = 10.16 LFVALEE425 pKa = 4.14 PGEE428 pKa = 4.12 YY429 pKa = 9.69 AIQVVAPDD437 pKa = 3.68 GTVFTSQNQGMDD449 pKa = 3.79 DD450 pKa = 4.51 DD451 pKa = 5.18 RR452 pKa = 11.84 DD453 pKa = 3.67 SDD455 pKa = 4.0 VASNGVSEE463 pKa = 4.6 FTTLTAGEE471 pKa = 4.86 NDD473 pKa = 4.01 LSWDD477 pKa = 3.44 AGLVPTGTIDD487 pKa = 2.85 IEE489 pKa = 4.27 KK490 pKa = 9.71 FVRR493 pKa = 11.84 IEE495 pKa = 3.97 TPGDD499 pKa = 3.51 FFEE502 pKa = 5.88 GAVCDD507 pKa = 4.05 EE508 pKa = 4.57 FGKK511 pKa = 9.37 ATALTFDD518 pKa = 4.27 YY519 pKa = 11.12 EE520 pKa = 4.13 EE521 pKa = 4.31 STNVSTSQDD530 pKa = 3.05 SSKK533 pKa = 11.53 AKK535 pKa = 10.17 ILTGTTFDD543 pKa = 4.51 GDD545 pKa = 3.54 GKK547 pKa = 10.99 SYY549 pKa = 11.27 VIVTDD554 pKa = 3.93 KK555 pKa = 11.31 SDD557 pKa = 3.42 PTDD560 pKa = 3.64 LGGKK564 pKa = 9.39 VYY566 pKa = 10.45 FSGYY570 pKa = 8.07 VVEE573 pKa = 5.39 GEE575 pKa = 4.43 EE576 pKa = 4.34 FTATAADD583 pKa = 3.55 AGADD587 pKa = 3.31 RR588 pKa = 11.84 FGSNTFVHH596 pKa = 7.02 FFDD599 pKa = 6.39 DD600 pKa = 3.97 NPFDD604 pKa = 4.16 NVVGDD609 pKa = 4.69 DD610 pKa = 5.7 DD611 pKa = 5.46 LLQSLTYY618 pKa = 8.87 HH619 pKa = 6.46 TSCSQPIYY627 pKa = 10.76 LGDD630 pKa = 3.46 QVGNVTLVGYY640 pKa = 10.02 SGEE643 pKa = 4.35 SGTAPSSPPVIGPDD657 pKa = 3.48 LDD659 pKa = 4.96 ADD661 pKa = 4.75 TIAEE665 pKa = 4.41 GPIGTIGTDD674 pKa = 3.29 TAVFTYY680 pKa = 10.07 KK681 pKa = 10.5 VKK683 pKa = 9.67 NTGTSDD689 pKa = 3.66 LEE691 pKa = 4.37 NVLVTDD697 pKa = 4.61 DD698 pKa = 4.41 RR699 pKa = 11.84 LSEE702 pKa = 3.89 LTLINKK708 pKa = 9.62 GDD710 pKa = 3.91 GDD712 pKa = 3.86 NLLEE716 pKa = 4.9 VGEE719 pKa = 4.32 TWIYY723 pKa = 8.19 TASEE727 pKa = 4.01 AVLGGINTNIGKK739 pKa = 8.31 VTANVVGTQFGVNDD753 pKa = 3.55 QDD755 pKa = 3.31 AANYY759 pKa = 10.3 VGFDD763 pKa = 3.7 TPPSIDD769 pKa = 3.19 LEE771 pKa = 4.56 KK772 pKa = 9.58 YY773 pKa = 10.21 VKK775 pKa = 10.79 VEE777 pKa = 4.11 GYY779 pKa = 8.57 TPFVPMVCEE788 pKa = 4.27 TYY790 pKa = 10.66 GKK792 pKa = 8.53 PVEE795 pKa = 4.06 MTFVYY800 pKa = 10.33 EE801 pKa = 3.99 VDD803 pKa = 4.56 DD804 pKa = 4.87 DD805 pKa = 4.55 NEE807 pKa = 4.98 SISNTQEE814 pKa = 3.4 GKK816 pKa = 10.79 AKK818 pKa = 10.26 ILTGTTLDD826 pKa = 3.86 NDD828 pKa = 3.46 DD829 pKa = 4.45 LAWVRR834 pKa = 11.84 VTDD837 pKa = 3.53 SDD839 pKa = 4.03 KK840 pKa = 11.62 AFDD843 pKa = 4.19 GGGKK847 pKa = 9.27 IYY849 pKa = 10.8 FEE851 pKa = 4.18 GLVAKK856 pKa = 10.35 GADD859 pKa = 3.83 FTATAALAGADD870 pKa = 3.42 RR871 pKa = 11.84 FASSTYY877 pKa = 9.54 IHH879 pKa = 6.56 YY880 pKa = 10.61 FDD882 pKa = 6.11 DD883 pKa = 4.16 EE884 pKa = 5.66 ADD886 pKa = 3.62 FTTEE890 pKa = 3.55 NSGGLLQSLTYY901 pKa = 8.9 HH902 pKa = 6.35 TSCSKK907 pKa = 10.57 PINLGDD913 pKa = 3.38 EE914 pKa = 3.88 TGYY917 pKa = 11.38 AKK919 pKa = 10.73 LVGYY923 pKa = 10.21 VGEE926 pKa = 5.26 DD927 pKa = 3.45 GAAEE931 pKa = 3.89 PVNEE935 pKa = 4.21 YY936 pKa = 11.28 GEE938 pKa = 4.79 DD939 pKa = 3.69 ADD941 pKa = 4.59 EE942 pKa = 5.2 GPGPEE947 pKa = 3.95 AAFGSTVEE955 pKa = 4.05 FTYY958 pKa = 11.06 VVTNTGSTALGQIEE972 pKa = 4.85 LSDD975 pKa = 3.97 DD976 pKa = 4.29 RR977 pKa = 11.84 ISGLTLVGGDD987 pKa = 4.22 DD988 pKa = 5.53 DD989 pKa = 5.81 GDD991 pKa = 3.75 NLLDD995 pKa = 4.91 VGEE998 pKa = 4.33 EE999 pKa = 3.98 WLFTGSEE1006 pKa = 4.33 TAVSGQVTNIGKK1018 pKa = 8.44 VTGTAVDD1025 pKa = 3.55 EE1026 pKa = 4.93 KK1027 pKa = 11.16 GNSLNLPTVTDD1038 pKa = 4.05 SDD1040 pKa = 3.72 PGNYY1044 pKa = 9.07 VVVGGPSIDD1053 pKa = 3.29 VEE1055 pKa = 4.49 KK1056 pKa = 10.55 YY1057 pKa = 9.61 VKK1059 pKa = 10.74 VEE1061 pKa = 4.04 GGGVPYY1067 pKa = 10.28 EE1068 pKa = 4.15 ALVCDD1073 pKa = 4.5 TYY1075 pKa = 11.86 GKK1077 pKa = 10.33 ALAMTFRR1084 pKa = 11.84 YY1085 pKa = 10.28 LEE1087 pKa = 4.43 GSTVDD1092 pKa = 3.29 TDD1094 pKa = 3.42 QDD1096 pKa = 3.57 SGKK1099 pKa = 10.88 AYY1101 pKa = 9.52 IDD1103 pKa = 3.91 KK1104 pKa = 11.01 DD1105 pKa = 4.12 LGTDD1109 pKa = 3.61 DD1110 pKa = 6.02 DD1111 pKa = 4.56 GVSYY1115 pKa = 10.69 IIVSDD1120 pKa = 3.6 KK1121 pKa = 11.21 SSPTDD1126 pKa = 3.41 LSGKK1130 pKa = 10.06 VYY1132 pKa = 10.34 FQGEE1136 pKa = 4.39 VAKK1139 pKa = 10.78 GADD1142 pKa = 3.94 FTALGASSFSSSTYY1156 pKa = 9.35 IHH1158 pKa = 6.58 YY1159 pKa = 10.64 FDD1161 pKa = 6.1 DD1162 pKa = 4.33 SPFDD1166 pKa = 3.82 GEE1168 pKa = 4.48 GSIDD1172 pKa = 3.87 LLQSSAYY1179 pKa = 8.26 HH1180 pKa = 6.0 TSCSQPINIGDD1191 pKa = 3.77 VVGNTTLVGYY1201 pKa = 10.27 LGEE1204 pKa = 5.09 DD1205 pKa = 3.91 GEE1207 pKa = 4.79 ANPTGSNDD1215 pKa = 3.88 GLGDD1219 pKa = 4.48 DD1220 pKa = 5.35 ADD1222 pKa = 4.25 TGPGPDD1228 pKa = 3.44 AAFGSDD1234 pKa = 3.27 VVFNYY1239 pKa = 10.03 VVTNTGSGALKK1250 pKa = 10.68 NVVLTDD1256 pKa = 3.69 DD1257 pKa = 5.06 RR1258 pKa = 11.84 ISDD1261 pKa = 3.6 VTYY1264 pKa = 11.07 VSGDD1268 pKa = 3.71 DD1269 pKa = 3.66 GDD1271 pKa = 4.27 KK1272 pKa = 10.82 LLEE1275 pKa = 4.43 VGEE1278 pKa = 4.26 EE1279 pKa = 3.8 WLYY1282 pKa = 10.27 TATEE1286 pKa = 4.06 KK1287 pKa = 10.86 AGQGVIGNIGKK1298 pKa = 8.04 VTAKK1302 pKa = 10.45 DD1303 pKa = 3.25 AATGTISVMDD1313 pKa = 4.32 KK1314 pKa = 10.98 DD1315 pKa = 3.46 AAFYY1319 pKa = 10.75 NGSAPGITLGDD1330 pKa = 3.26 RR1331 pKa = 11.84 VWFDD1335 pKa = 3.83 KK1336 pKa = 11.42 NCDD1339 pKa = 3.67 GIQDD1343 pKa = 4.03 DD1344 pKa = 5.14 GEE1346 pKa = 4.85 NGAAGITVNLMNSVGNVIATTTTDD1370 pKa = 2.49 VDD1372 pKa = 3.71 GNYY1375 pKa = 10.36 LFTDD1379 pKa = 4.73 LAPGTYY1385 pKa = 9.85 SVGFVAPNGYY1395 pKa = 9.66 IFTKK1399 pKa = 10.44 QDD1401 pKa = 2.76 ATADD1405 pKa = 4.01 DD1406 pKa = 4.11 VDD1408 pKa = 4.49 SDD1410 pKa = 4.26 ANANGLTGQYY1420 pKa = 10.37 KK1421 pKa = 10.33 LAGGEE1426 pKa = 4.23 TNLTVDD1432 pKa = 3.28 AGLVEE1437 pKa = 4.8 FTTKK1441 pKa = 10.54 DD1442 pKa = 3.17 VSFSFNGSSSGSGTYY1457 pKa = 11.04 GNIRR1461 pKa = 11.84 TYY1463 pKa = 10.36 TDD1465 pKa = 2.7 NGVSVNVSAHH1475 pKa = 5.22 SRR1477 pKa = 11.84 SDD1479 pKa = 3.56 KK1480 pKa = 11.22 SGDD1483 pKa = 2.86 WSTSYY1488 pKa = 11.07 LGAFSGGMGVTNRR1501 pKa = 11.84 NEE1503 pKa = 4.17 SGSGNSHH1510 pKa = 5.37 TVDD1513 pKa = 3.24 NRR1515 pKa = 11.84 GDD1517 pKa = 3.29 MDD1519 pKa = 5.16 DD1520 pKa = 3.79 YY1521 pKa = 11.92 VLFEE1525 pKa = 4.13 FDD1527 pKa = 3.47 QNVVIDD1533 pKa = 4.13 SAFLGYY1539 pKa = 10.6 VVDD1542 pKa = 5.0 DD1543 pKa = 3.91 SDD1545 pKa = 3.6 MSVWIGTLDD1554 pKa = 3.41 NAFNNHH1560 pKa = 5.4 TNLSDD1565 pKa = 5.94 DD1566 pKa = 4.41 ILSQMGFTEE1575 pKa = 4.34 INN1577 pKa = 3.09
Molecular weight: 165.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.692
IPC2_protein 3.605
IPC_protein 3.668
Toseland 3.427
ProMoST 3.821
Dawson 3.656
Bjellqvist 3.808
Wikipedia 3.605
Rodwell 3.478
Grimsley 3.325
Solomon 3.656
Lehninger 3.617
Nozaki 3.757
DTASelect 4.05
Thurlkill 3.478
EMBOSS 3.605
Sillero 3.783
Patrickios 1.189
IPC_peptide 3.656
IPC2_peptide 3.757
IPC2.peptide.svr19 3.708
Protein with the highest isoelectric point:
>tr|A0A1I2LQ04|A0A1I2LQ04_9GAMM NADP-dependent 3-hydroxy acid dehydrogenase YdfG OS=Neptunomonas qingdaonensis OX=1045558 GN=SAMN05216175_101181 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.49 RR12 pKa = 11.84 ARR14 pKa = 11.84 THH16 pKa = 5.91 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.31 SGRR28 pKa = 11.84 AVINRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.27 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LSAA44 pKa = 3.91
Molecular weight: 5.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.647
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 12.998
DTASelect 12.998
Thurlkill 12.998
EMBOSS 13.51
Sillero 12.998
Patrickios 12.369
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.185
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4011
0
4011
1312960
24
7596
327.3
36.23
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.019 ± 0.049
1.027 ± 0.015
5.611 ± 0.052
6.08 ± 0.045
3.931 ± 0.025
7.001 ± 0.047
2.264 ± 0.022
6.43 ± 0.03
4.824 ± 0.042
10.735 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.596 ± 0.028
3.922 ± 0.039
4.081 ± 0.027
4.477 ± 0.031
4.964 ± 0.042
6.714 ± 0.042
5.321 ± 0.063
6.928 ± 0.033
1.241 ± 0.016
2.833 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here