Microbacterium sp. Gd 4-13

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Microbacterium; unclassified Microbacterium

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3162 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2U0H0I4|A0A2U0H0I4_9MICO FMN-binding protein OS=Microbacterium sp. Gd 4-13 OX=2173179 GN=DEA06_15820 PE=4 SV=1
MM1 pKa = 6.86TRR3 pKa = 11.84VRR5 pKa = 11.84SRR7 pKa = 11.84IAVAVAVLTTVVLVASGCSVFTSDD31 pKa = 4.27SWAPPPSVTPDD42 pKa = 3.31VSGVSADD49 pKa = 4.13LLPFYY54 pKa = 10.63DD55 pKa = 5.25QDD57 pKa = 4.27VAWEE61 pKa = 4.23SCAAEE66 pKa = 4.02QQFDD70 pKa = 4.03CANVRR75 pKa = 11.84VPRR78 pKa = 11.84DD79 pKa = 3.1WSAPASGEE87 pKa = 3.69IEE89 pKa = 3.84IAVIRR94 pKa = 11.84HH95 pKa = 5.81RR96 pKa = 11.84ADD98 pKa = 2.73SGAPIGSLFTNPGGPGVSGVDD119 pKa = 3.44TLKK122 pKa = 10.7QALDD126 pKa = 3.8VVAGSPLRR134 pKa = 11.84EE135 pKa = 4.09NYY137 pKa = 10.19DD138 pKa = 3.32VIGFDD143 pKa = 3.56PRR145 pKa = 11.84GVGASTAIDD154 pKa = 4.24CYY156 pKa = 10.8DD157 pKa = 3.54TADD160 pKa = 4.17LDD162 pKa = 3.67QYY164 pKa = 11.54LYY166 pKa = 10.24DD167 pKa = 4.61IPDD170 pKa = 3.67APRR173 pKa = 11.84GSDD176 pKa = 2.58EE177 pKa = 3.7WTAEE181 pKa = 3.52IEE183 pKa = 4.19ARR185 pKa = 11.84DD186 pKa = 3.64AAFAAACDD194 pKa = 3.97ANSDD198 pKa = 3.66GLLPFVSTEE207 pKa = 3.47NAARR211 pKa = 11.84DD212 pKa = 3.92LDD214 pKa = 3.83VLRR217 pKa = 11.84AVMGEE222 pKa = 4.0TTLDD226 pKa = 3.37YY227 pKa = 11.17LGYY230 pKa = 10.35SWGTALGAAYY240 pKa = 10.32AEE242 pKa = 4.19LHH244 pKa = 6.65PDD246 pKa = 2.81RR247 pKa = 11.84VGRR250 pKa = 11.84MVLDD254 pKa = 4.26GALDD258 pKa = 3.74PSIPGAEE265 pKa = 3.97VGVGQMEE272 pKa = 4.91GFQRR276 pKa = 11.84SLDD279 pKa = 3.76AFLTACLSFDD289 pKa = 3.68DD290 pKa = 4.66CPFRR294 pKa = 11.84GTPAEE299 pKa = 4.06ASADD303 pKa = 3.71LDD305 pKa = 3.82ALFASVDD312 pKa = 3.29ARR314 pKa = 11.84PIVAADD320 pKa = 3.47GRR322 pKa = 11.84EE323 pKa = 4.03LGADD327 pKa = 3.42TLMLALLNALYY338 pKa = 10.66SPASWPYY345 pKa = 11.22LRR347 pKa = 11.84VTLSGVQNADD357 pKa = 3.47PAPAFTLADD366 pKa = 4.08AYY368 pKa = 10.77NARR371 pKa = 11.84QGGDD375 pKa = 3.22YY376 pKa = 10.83VGNSEE381 pKa = 4.61EE382 pKa = 5.03AFPAYY387 pKa = 11.02NCMDD391 pKa = 3.66YY392 pKa = 10.48PAVGEE397 pKa = 4.41AQTQAAQAEE406 pKa = 4.58LEE408 pKa = 4.32RR409 pKa = 11.84VAPLAAEE416 pKa = 4.35YY417 pKa = 10.57FSGPDD422 pKa = 3.09VCEE425 pKa = 3.67QWPYY429 pKa = 10.83PPTGTRR435 pKa = 11.84GPVSADD441 pKa = 2.7GAAPILVIGTTSDD454 pKa = 3.36PATPYY459 pKa = 9.9QWAVSLAEE467 pKa = 3.92QLTSGVLVTRR477 pKa = 11.84VGEE480 pKa = 4.08GHH482 pKa = 6.17TGYY485 pKa = 11.13NKK487 pKa = 10.76GNTCVDD493 pKa = 4.01DD494 pKa = 4.58AVVAYY499 pKa = 10.15LVDD502 pKa = 4.1GVVPDD507 pKa = 3.83ADD509 pKa = 3.63IRR511 pKa = 11.84CEE513 pKa = 3.76

Molecular weight: 53.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2U0H5U6|A0A2U0H5U6_9MICO Uncharacterized protein OS=Microbacterium sp. Gd 4-13 OX=2173179 GN=DEA06_09495 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3162

0

3162

1028731

29

2440

325.3

34.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.739 ± 0.058

0.471 ± 0.009

6.445 ± 0.042

5.547 ± 0.038

3.087 ± 0.027

8.935 ± 0.034

1.91 ± 0.021

4.452 ± 0.031

1.754 ± 0.031

9.87 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.719 ± 0.016

1.89 ± 0.026

5.45 ± 0.03

2.625 ± 0.023

7.535 ± 0.056

5.772 ± 0.028

6.13 ± 0.041

9.228 ± 0.044

1.513 ± 0.018

1.929 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski