Amino acid dipepetide frequency for Microbacterium sp. Gd 4-13

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.2AlaAla: 21.2 ± 0.229	0.618AlaCys: 0.618 ± 0.026	8.893AlaAsp: 8.893 ± 0.121	7.891AlaGlu: 7.891 ± 0.103	3.995AlaPhe: 3.995 ± 0.072	12.179AlaGly: 12.179 ± 0.138	2.408AlaHis: 2.408 ± 0.048	6.013AlaIle: 6.013 ± 0.09	2.398AlaLys: 2.398 ± 0.058	14.232AlaLeu: 14.232 ± 0.15	2.642AlaMet: 2.642 ± 0.053	2.23AlaAsn: 2.23 ± 0.055	6.874AlaPro: 6.874 ± 0.11	3.723AlaGln: 3.723 ± 0.07	9.834AlaArg: 9.834 ± 0.119	7.985AlaSer: 7.985 ± 0.094	7.68AlaThr: 7.68 ± 0.101	12.157AlaVal: 12.157 ± 0.135	1.978AlaTrp: 1.978 ± 0.041	2.461AlaTyr: 2.461 ± 0.05	0.0AlaXaa: 0.0 ± 0.0
Cys	0.637CysAla: 0.637 ± 0.026	0.043CysCys: 0.043 ± 0.007	0.291CysAsp: 0.291 ± 0.016	0.243CysGlu: 0.243 ± 0.016	0.168CysPhe: 0.168 ± 0.013	0.528CysGly: 0.528 ± 0.023	0.113CysHis: 0.113 ± 0.012	0.184CysIle: 0.184 ± 0.012	0.045CysLys: 0.045 ± 0.006	0.4CysLeu: 0.4 ± 0.02	0.064CysMet: 0.064 ± 0.008	0.079CysAsn: 0.079 ± 0.01	0.272CysPro: 0.272 ± 0.014	0.123CysGln: 0.123 ± 0.012	0.334CysArg: 0.334 ± 0.019	0.296CysSer: 0.296 ± 0.017	0.308CysThr: 0.308 ± 0.019	0.436CysVal: 0.436 ± 0.02	0.058CysTrp: 0.058 ± 0.007	0.085CysTyr: 0.085 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	10.02AspAla: 10.02 ± 0.124	0.224AspCys: 0.224 ± 0.016	4.922AspAsp: 4.922 ± 0.089	4.415AspGlu: 4.415 ± 0.079	1.674AspPhe: 1.674 ± 0.046	6.318AspGly: 6.318 ± 0.112	1.209AspHis: 1.209 ± 0.038	2.327AspIle: 2.327 ± 0.047	0.905AspLys: 0.905 ± 0.033	6.506AspLeu: 6.506 ± 0.079	0.791AspMet: 0.791 ± 0.028	0.996AspAsn: 0.996 ± 0.039	4.32AspPro: 4.32 ± 0.065	1.51AspGln: 1.51 ± 0.041	4.701AspArg: 4.701 ± 0.07	2.629AspSer: 2.629 ± 0.049	3.108AspThr: 3.108 ± 0.05	5.74AspVal: 5.74 ± 0.084	0.871AspTrp: 0.871 ± 0.031	1.268AspTyr: 1.268 ± 0.036	0.0AspXaa: 0.0 ± 0.0
Glu	7.134GluAla: 7.134 ± 0.082	0.222GluCys: 0.222 ± 0.016	2.48GluAsp: 2.48 ± 0.049	2.741GluGlu: 2.741 ± 0.059	1.644GluPhe: 1.644 ± 0.044	4.158GluGly: 4.158 ± 0.064	1.338GluHis: 1.338 ± 0.035	2.858GluIle: 2.858 ± 0.052	1.326GluLys: 1.326 ± 0.047	6.047GluLeu: 6.047 ± 0.095	0.956GluMet: 0.956 ± 0.035	1.278GluAsn: 1.278 ± 0.04	3.123GluPro: 3.123 ± 0.069	2.0GluGln: 2.0 ± 0.047	5.196GluArg: 5.196 ± 0.088	2.926GluSer: 2.926 ± 0.055	3.186GluThr: 3.186 ± 0.05	4.696GluVal: 4.696 ± 0.079	0.967GluTrp: 0.967 ± 0.033	1.198GluTyr: 1.198 ± 0.036	0.0GluXaa: 0.0 ± 0.0
Phe	4.486PheAla: 4.486 ± 0.074	0.141PheCys: 0.141 ± 0.012	2.519PheAsp: 2.519 ± 0.051	1.653PheGlu: 1.653 ± 0.046	1.091PhePhe: 1.091 ± 0.035	3.341PheGly: 3.341 ± 0.058	0.53PheHis: 0.53 ± 0.025	1.099PheIle: 1.099 ± 0.031	0.371PheLys: 0.371 ± 0.019	2.802PheLeu: 2.802 ± 0.053	0.366PheMet: 0.366 ± 0.021	0.535PheAsn: 0.535 ± 0.026	1.346PhePro: 1.346 ± 0.035	0.724PheGln: 0.724 ± 0.031	1.824PheArg: 1.824 ± 0.042	1.783PheSer: 1.783 ± 0.044	2.118PheThr: 2.118 ± 0.051	3.023PheVal: 3.023 ± 0.06	0.5PheTrp: 0.5 ± 0.022	0.613PheTyr: 0.613 ± 0.029	0.0PheXaa: 0.0 ± 0.0
Gly	11.181GlyAla: 11.181 ± 0.113	0.565GlyCys: 0.565 ± 0.026	5.378GlyAsp: 5.378 ± 0.078	5.111GlyGlu: 5.111 ± 0.081	3.252GlyPhe: 3.252 ± 0.073	7.877GlyGly: 7.877 ± 0.126	1.715GlyHis: 1.715 ± 0.045	4.573GlyIle: 4.573 ± 0.064	1.877GlyLys: 1.877 ± 0.048	8.63GlyLeu: 8.63 ± 0.105	1.956GlyMet: 1.956 ± 0.048	1.65GlyAsn: 1.65 ± 0.045	3.744GlyPro: 3.744 ± 0.062	2.369GlyGln: 2.369 ± 0.051	6.504GlyArg: 6.504 ± 0.09	5.467GlySer: 5.467 ± 0.082	5.475GlyThr: 5.475 ± 0.089	8.299GlyVal: 8.299 ± 0.099	1.621GlyTrp: 1.621 ± 0.038	2.112GlyTyr: 2.112 ± 0.043	0.0GlyXaa: 0.0 ± 0.0
His	2.253HisAla: 2.253 ± 0.045	0.109HisCys: 0.109 ± 0.011	1.346HisAsp: 1.346 ± 0.044	1.058HisGlu: 1.058 ± 0.029	0.499HisPhe: 0.499 ± 0.022	1.864HisGly: 1.864 ± 0.044	0.557HisHis: 0.557 ± 0.027	0.685HisIle: 0.685 ± 0.024	0.243HisLys: 0.243 ± 0.016	1.981HisLeu: 1.981 ± 0.05	0.29HisMet: 0.29 ± 0.017	0.332HisAsn: 0.332 ± 0.017	1.546HisPro: 1.546 ± 0.046	0.486HisGln: 0.486 ± 0.02	1.647HisArg: 1.647 ± 0.048	0.899HisSer: 0.899 ± 0.03	0.974HisThr: 0.974 ± 0.03	1.677HisVal: 1.677 ± 0.035	0.288HisTrp: 0.288 ± 0.017	0.365HisTyr: 0.365 ± 0.021	0.0HisXaa: 0.0 ± 0.0
Ile	7.076IleAla: 7.076 ± 0.082	0.234IleCys: 0.234 ± 0.016	3.665IleAsp: 3.665 ± 0.056	2.852IleGlu: 2.852 ± 0.064	1.096IlePhe: 1.096 ± 0.04	4.566IleGly: 4.566 ± 0.074	0.659IleHis: 0.659 ± 0.027	1.661IleIle: 1.661 ± 0.052	0.647IleLys: 0.647 ± 0.026	3.566IleLeu: 3.566 ± 0.075	0.556IleMet: 0.556 ± 0.021	0.824IleAsn: 0.824 ± 0.03	2.351IlePro: 2.351 ± 0.055	0.937IleGln: 0.937 ± 0.032	2.682IleArg: 2.682 ± 0.051	2.395IleSer: 2.395 ± 0.049	2.682IleThr: 2.682 ± 0.058	4.878IleVal: 4.878 ± 0.084	0.466IleTrp: 0.466 ± 0.022	0.723IleTyr: 0.723 ± 0.027	0.0IleXaa: 0.0 ± 0.0
Lys	2.123LysAla: 2.123 ± 0.065	0.048LysCys: 0.048 ± 0.007	0.969LysAsp: 0.969 ± 0.034	0.847LysGlu: 0.847 ± 0.034	0.394LysPhe: 0.394 ± 0.022	1.427LysGly: 1.427 ± 0.039	0.388LysHis: 0.388 ± 0.021	0.872LysIle: 0.872 ± 0.029	0.675LysLys: 0.675 ± 0.038	1.611LysLeu: 1.611 ± 0.043	0.333LysMet: 0.333 ± 0.018	0.46LysAsn: 0.46 ± 0.024	1.006LysPro: 1.006 ± 0.038	0.561LysGln: 0.561 ± 0.025	1.385LysArg: 1.385 ± 0.045	1.004LysSer: 1.004 ± 0.033	1.288LysThr: 1.288 ± 0.043	1.535LysVal: 1.535 ± 0.043	0.224LysTrp: 0.224 ± 0.016	0.392LysTyr: 0.392 ± 0.02	0.0LysXaa: 0.0 ± 0.0
Leu	14.378LeuAla: 14.378 ± 0.158	0.427LeuCys: 0.427 ± 0.021	6.71LeuAsp: 6.71 ± 0.086	5.03LeuGlu: 5.03 ± 0.08	2.889LeuPhe: 2.889 ± 0.062	8.869LeuGly: 8.869 ± 0.103	1.805LeuHis: 1.805 ± 0.044	4.211LeuIle: 4.211 ± 0.079	1.45LeuLys: 1.45 ± 0.043	9.588LeuLeu: 9.588 ± 0.137	1.521LeuMet: 1.521 ± 0.041	1.672LeuAsn: 1.672 ± 0.042	5.567LeuPro: 5.567 ± 0.083	2.426LeuGln: 2.426 ± 0.057	7.711LeuArg: 7.711 ± 0.106	5.911LeuSer: 5.911 ± 0.084	6.241LeuThr: 6.241 ± 0.075	9.488LeuVal: 9.488 ± 0.114	1.244LeuTrp: 1.244 ± 0.037	1.558LeuTyr: 1.558 ± 0.045	0.0LeuXaa: 0.0 ± 0.0
Met	1.933MetAla: 1.933 ± 0.044	0.079MetCys: 0.079 ± 0.009	0.816MetAsp: 0.816 ± 0.03	0.56MetGlu: 0.56 ± 0.027	0.486MetPhe: 0.486 ± 0.022	1.284MetGly: 1.284 ± 0.04	0.294MetHis: 0.294 ± 0.018	0.793MetIle: 0.793 ± 0.031	0.377MetLys: 0.377 ± 0.019	1.967MetLeu: 1.967 ± 0.048	0.298MetMet: 0.298 ± 0.018	0.479MetAsn: 0.479 ± 0.023	1.081MetPro: 1.081 ± 0.033	0.47MetGln: 0.47 ± 0.022	1.449MetArg: 1.449 ± 0.042	1.384MetSer: 1.384 ± 0.033	1.691MetThr: 1.691 ± 0.039	1.26MetVal: 1.26 ± 0.031	0.22MetTrp: 0.22 ± 0.015	0.268MetTyr: 0.268 ± 0.016	0.0MetXaa: 0.0 ± 0.0
Asn	2.485AsnAla: 2.485 ± 0.05	0.109AsnCys: 0.109 ± 0.01	1.222AsnAsp: 1.222 ± 0.04	0.928AsnGlu: 0.928 ± 0.028	0.583AsnPhe: 0.583 ± 0.024	1.791AsnGly: 1.791 ± 0.041	0.316AsnHis: 0.316 ± 0.017	0.819AsnIle: 0.819 ± 0.029	0.365AsnLys: 0.365 ± 0.019	1.828AsnLeu: 1.828 ± 0.039	0.29AsnMet: 0.29 ± 0.019	0.441AsnAsn: 0.441 ± 0.024	1.553AsnPro: 1.553 ± 0.041	0.474AsnGln: 0.474 ± 0.024	1.209AsnArg: 1.209 ± 0.037	0.976AsnSer: 0.976 ± 0.039	1.135AsnThr: 1.135 ± 0.039	1.653AsnVal: 1.653 ± 0.042	0.292AsnTrp: 0.292 ± 0.019	0.427AsnTyr: 0.427 ± 0.02	0.0AsnXaa: 0.0 ± 0.0
Pro	7.266ProAla: 7.266 ± 0.103	0.191ProCys: 0.191 ± 0.013	3.88ProAsp: 3.88 ± 0.066	3.794ProGlu: 3.794 ± 0.064	1.858ProPhe: 1.858 ± 0.039	5.12ProGly: 5.12 ± 0.081	1.116ProHis: 1.116 ± 0.034	2.088ProIle: 2.088 ± 0.048	0.888ProLys: 0.888 ± 0.039	4.905ProLeu: 4.905 ± 0.076	0.804ProMet: 0.804 ± 0.026	0.988ProAsn: 0.988 ± 0.036	2.511ProPro: 2.511 ± 0.07	1.425ProGln: 1.425 ± 0.042	3.79ProArg: 3.79 ± 0.073	3.37ProSer: 3.37 ± 0.055	3.539ProThr: 3.539 ± 0.067	5.067ProVal: 5.067 ± 0.069	0.906ProTrp: 0.906 ± 0.031	0.992ProTyr: 0.992 ± 0.031	0.0ProXaa: 0.0 ± 0.0
Gln	3.254GlnAla: 3.254 ± 0.069	0.1GlnCys: 0.1 ± 0.011	1.14GlnAsp: 1.14 ± 0.033	1.201GlnGlu: 1.201 ± 0.038	0.836GlnPhe: 0.836 ± 0.03	1.981GlnGly: 1.981 ± 0.044	0.654GlnHis: 0.654 ± 0.026	1.347GlnIle: 1.347 ± 0.041	0.588GlnLys: 0.588 ± 0.029	2.905GlnLeu: 2.905 ± 0.061	0.473GlnMet: 0.473 ± 0.021	0.628GlnAsn: 0.628 ± 0.028	1.438GlnPro: 1.438 ± 0.037	1.04GlnGln: 1.04 ± 0.038	2.384GlnArg: 2.384 ± 0.052	1.445GlnSer: 1.445 ± 0.039	1.609GlnThr: 1.609 ± 0.041	2.197GlnVal: 2.197 ± 0.05	0.462GlnTrp: 0.462 ± 0.022	0.568GlnTyr: 0.568 ± 0.026	0.0GlnXaa: 0.0 ± 0.0
Arg	9.726ArgAla: 9.726 ± 0.138	0.318ArgCys: 0.318 ± 0.02	4.507ArgAsp: 4.507 ± 0.075	4.43ArgGlu: 4.43 ± 0.08	2.406ArgPhe: 2.406 ± 0.045	5.86ArgGly: 5.86 ± 0.087	1.584ArgHis: 1.584 ± 0.043	3.652ArgIle: 3.652 ± 0.057	1.291ArgLys: 1.291 ± 0.036	7.316ArgLeu: 7.316 ± 0.095	1.763ArgMet: 1.763 ± 0.042	1.329ArgAsn: 1.329 ± 0.034	3.867ArgPro: 3.867 ± 0.073	1.934ArgGln: 1.934 ± 0.049	7.248ArgArg: 7.248 ± 0.11	4.456ArgSer: 4.456 ± 0.065	4.38ArgThr: 4.38 ± 0.067	6.488ArgVal: 6.488 ± 0.074	1.286ArgTrp: 1.286 ± 0.037	1.512ArgTyr: 1.512 ± 0.038	0.0ArgXaa: 0.0 ± 0.0
Ser	7.553SerAla: 7.553 ± 0.094	0.25SerCys: 0.25 ± 0.017	3.58SerAsp: 3.58 ± 0.056	2.84SerGlu: 2.84 ± 0.056	1.928SerPhe: 1.928 ± 0.046	5.826SerGly: 5.826 ± 0.084	1.026SerHis: 1.026 ± 0.033	2.764SerIle: 2.764 ± 0.063	0.936SerLys: 0.936 ± 0.037	5.421SerLeu: 5.421 ± 0.086	1.147SerMet: 1.147 ± 0.034	1.111SerAsn: 1.111 ± 0.034	3.229SerPro: 3.229 ± 0.061	1.401SerGln: 1.401 ± 0.035	4.071SerArg: 4.071 ± 0.068	3.703SerSer: 3.703 ± 0.075	3.808SerThr: 3.808 ± 0.072	4.999SerVal: 4.999 ± 0.075	0.957SerTrp: 0.957 ± 0.032	1.17SerTyr: 1.17 ± 0.035	0.0SerXaa: 0.0 ± 0.0
Thr	8.061ThrAla: 8.061 ± 0.102	0.304ThrCys: 0.304 ± 0.018	3.716ThrAsp: 3.716 ± 0.059	3.034ThrGlu: 3.034 ± 0.059	1.943ThrPhe: 1.943 ± 0.049	5.792ThrGly: 5.792 ± 0.079	1.118ThrHis: 1.118 ± 0.035	2.868ThrIle: 2.868 ± 0.053	1.081ThrLys: 1.081 ± 0.036	5.966ThrLeu: 5.966 ± 0.072	0.917ThrMet: 0.917 ± 0.031	1.176ThrAsn: 1.176 ± 0.041	4.09ThrPro: 4.09 ± 0.078	1.465ThrGln: 1.465 ± 0.044	3.921ThrArg: 3.921 ± 0.063	3.639ThrSer: 3.639 ± 0.068	3.953ThrThr: 3.953 ± 0.072	6.093ThrVal: 6.093 ± 0.09	0.892ThrTrp: 0.892 ± 0.031	1.269ThrTyr: 1.269 ± 0.038	0.0ThrXaa: 0.0 ± 0.0
Val	12.577ValAla: 12.577 ± 0.125	0.511ValCys: 0.511 ± 0.026	6.262ValAsp: 6.262 ± 0.087	5.119ValGlu: 5.119 ± 0.079	2.891ValPhe: 2.891 ± 0.059	7.536ValGly: 7.536 ± 0.095	1.648ValHis: 1.648 ± 0.038	4.325ValIle: 4.325 ± 0.081	1.477ValLys: 1.477 ± 0.038	9.341ValLeu: 9.341 ± 0.113	1.411ValMet: 1.411 ± 0.045	1.793ValAsn: 1.793 ± 0.046	4.8ValPro: 4.8 ± 0.073	2.138ValGln: 2.138 ± 0.049	6.526ValArg: 6.526 ± 0.088	5.386ValSer: 5.386 ± 0.079	6.02ValThr: 6.02 ± 0.099	9.716ValVal: 9.716 ± 0.124	1.236ValTrp: 1.236 ± 0.035	1.567ValTyr: 1.567 ± 0.039	0.0ValXaa: 0.0 ± 0.0
Trp	1.646TrpAla: 1.646 ± 0.041	0.103TrpCys: 0.103 ± 0.01	0.788TrpAsp: 0.788 ± 0.028	0.627TrpGlu: 0.627 ± 0.024	0.57TrpPhe: 0.57 ± 0.023	1.159TrpGly: 1.159 ± 0.037	0.359TrpHis: 0.359 ± 0.02	0.717TrpIle: 0.717 ± 0.032	0.292TrpLys: 0.292 ± 0.017	1.637TrpLeu: 1.637 ± 0.042	0.362TrpMet: 0.362 ± 0.02	0.444TrpAsn: 0.444 ± 0.023	0.731TrpPro: 0.731 ± 0.028	0.513TrpGln: 0.513 ± 0.023	1.317TrpArg: 1.317 ± 0.038	0.97TrpSer: 0.97 ± 0.033	0.955TrpThr: 0.955 ± 0.035	1.261TrpVal: 1.261 ± 0.042	0.389TrpTrp: 0.389 ± 0.022	0.293TrpTyr: 0.293 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.404TyrAla: 2.404 ± 0.053	0.112TyrCys: 0.112 ± 0.011	1.372TyrAsp: 1.372 ± 0.037	1.098TyrGlu: 1.098 ± 0.036	0.664TyrPhe: 0.664 ± 0.027	1.88TyrGly: 1.88 ± 0.05	0.265TyrHis: 0.265 ± 0.016	0.66TyrIle: 0.66 ± 0.024	0.307TyrLys: 0.307 ± 0.017	2.051TyrLeu: 2.051 ± 0.039	0.248TyrMet: 0.248 ± 0.016	0.452TyrAsn: 0.452 ± 0.023	1.05TyrPro: 1.05 ± 0.034	0.531TyrGln: 0.531 ± 0.025	1.615TyrArg: 1.615 ± 0.044	1.094TyrSer: 1.094 ± 0.034	1.147TyrThr: 1.147 ± 0.031	1.617TyrVal: 1.617 ± 0.038	0.275TyrTrp: 0.275 ± 0.017	0.445TyrTyr: 0.445 ± 0.022	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3162 proteins (1028731 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---