Kurthia sp. 3B1D

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Planococcaceae; Kurthia; unclassified Kurthia

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3621 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A433RRF5|A0A433RRF5_9BACL Uncharacterized protein OS=Kurthia sp. 3B1D OX=1562256 GN=QI30_14325 PE=4 SV=1
MM1 pKa = 7.18YY2 pKa = 10.15LYY4 pKa = 10.83LIRR7 pKa = 11.84NVKK10 pKa = 10.42YY11 pKa = 9.99ILNGINLLFVSLKK24 pKa = 10.8ALFFINDD31 pKa = 3.63DD32 pKa = 4.11KK33 pKa = 11.44ILLEE37 pKa = 4.54RR38 pKa = 11.84GVGMEE43 pKa = 4.03WTVEE47 pKa = 4.3GYY49 pKa = 9.87KK50 pKa = 10.66ASLHH54 pKa = 5.54EE55 pKa = 4.1QFSQYY60 pKa = 9.84SAEE63 pKa = 4.16IIEE66 pKa = 4.45GFKK69 pKa = 10.47KK70 pKa = 10.53VKK72 pKa = 9.79QEE74 pKa = 4.12SYY76 pKa = 11.0SEE78 pKa = 4.41DD79 pKa = 3.46VALLQVMAFIGEE91 pKa = 4.23PTIDD95 pKa = 3.29MQLFFMADD103 pKa = 3.18MFDD106 pKa = 3.79EE107 pKa = 5.27AGTEE111 pKa = 3.94ALDD114 pKa = 3.88FLEE117 pKa = 5.99DD118 pKa = 3.57ALLDD122 pKa = 3.48KK123 pKa = 10.65WLDD126 pKa = 3.41VYY128 pKa = 11.31GYY130 pKa = 11.04GEE132 pKa = 4.3DD133 pKa = 3.61SEE135 pKa = 5.78AYY137 pKa = 9.87EE138 pKa = 3.98EE139 pKa = 4.64FYY141 pKa = 11.3EE142 pKa = 4.21EE143 pKa = 5.24NEE145 pKa = 4.28LEE147 pKa = 4.23DD148 pKa = 3.71VLIAEE153 pKa = 5.1LGQWIKK159 pKa = 11.5DD160 pKa = 3.86CFDD163 pKa = 3.62EE164 pKa = 5.95ANGSDD169 pKa = 3.33IRR171 pKa = 11.84YY172 pKa = 7.9PIYY175 pKa = 10.53FGVHH179 pKa = 6.97DD180 pKa = 5.05GPYY183 pKa = 9.85MLDD186 pKa = 2.93LHH188 pKa = 5.72TNEE191 pKa = 5.06WINPDD196 pKa = 3.54EE197 pKa = 4.81SDD199 pKa = 3.5EE200 pKa = 4.88DD201 pKa = 3.89EE202 pKa = 4.2

Molecular weight: 23.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A433RW82|A0A433RW82_9BACL YycI domain-containing protein OS=Kurthia sp. 3B1D OX=1562256 GN=QI30_05575 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.54QPKK8 pKa = 9.44KK9 pKa = 7.96RR10 pKa = 11.84KK11 pKa = 8.69HH12 pKa = 5.94SKK14 pKa = 8.54VHH16 pKa = 5.68GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 9.66NGRR28 pKa = 11.84RR29 pKa = 11.84VLAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.81GRR39 pKa = 11.84KK40 pKa = 8.75VLSAA44 pKa = 4.05

Molecular weight: 5.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3621

0

3621

1035375

27

2277

285.9

32.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.251 ± 0.045

0.691 ± 0.013

5.321 ± 0.038

7.134 ± 0.045

4.469 ± 0.031

6.357 ± 0.043

2.204 ± 0.02

7.354 ± 0.049

6.533 ± 0.047

9.665 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.919 ± 0.022

4.126 ± 0.024

3.439 ± 0.023

4.172 ± 0.036

4.013 ± 0.034

5.378 ± 0.029

6.139 ± 0.04

7.232 ± 0.038

0.925 ± 0.015

3.678 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski