Amino acid dipepetide frequency for Kurthia sp. 3B1D

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.162AlaAla: 7.162 ± 0.109	0.705AlaCys: 0.705 ± 0.028	4.022AlaAsp: 4.022 ± 0.071	5.071AlaGlu: 5.071 ± 0.078	3.895AlaPhe: 3.895 ± 0.073	5.34AlaGly: 5.34 ± 0.094	1.703AlaHis: 1.703 ± 0.039	6.759AlaIle: 6.759 ± 0.083	5.431AlaLys: 5.431 ± 0.089	8.676AlaLeu: 8.676 ± 0.101	2.503AlaMet: 2.503 ± 0.056	3.124AlaAsn: 3.124 ± 0.063	2.46AlaPro: 2.46 ± 0.058	3.467AlaGln: 3.467 ± 0.071	2.995AlaArg: 2.995 ± 0.057	4.514AlaSer: 4.514 ± 0.072	5.111AlaThr: 5.111 ± 0.076	6.065AlaVal: 6.065 ± 0.082	0.667AlaTrp: 0.667 ± 0.026	2.833AlaTyr: 2.833 ± 0.049	0.001AlaXaa: 0.001 ± 0.001
Cys	0.527CysAla: 0.527 ± 0.025	0.089CysCys: 0.089 ± 0.01	0.379CysAsp: 0.379 ± 0.02	0.497CysGlu: 0.497 ± 0.023	0.309CysPhe: 0.309 ± 0.019	0.685CysGly: 0.685 ± 0.026	0.205CysHis: 0.205 ± 0.015	0.508CysIle: 0.508 ± 0.023	0.289CysLys: 0.289 ± 0.016	0.6CysLeu: 0.6 ± 0.026	0.192CysMet: 0.192 ± 0.013	0.227CysAsn: 0.227 ± 0.014	0.293CysPro: 0.293 ± 0.02	0.21CysGln: 0.21 ± 0.016	0.272CysArg: 0.272 ± 0.018	0.43CysSer: 0.43 ± 0.022	0.413CysThr: 0.413 ± 0.019	0.443CysVal: 0.443 ± 0.021	0.061CysTrp: 0.061 ± 0.008	0.281CysTyr: 0.281 ± 0.016	0.0CysXaa: 0.0 ± 0.0
Asp	4.49AspAla: 4.49 ± 0.075	0.375AspCys: 0.375 ± 0.021	2.952AspAsp: 2.952 ± 0.064	4.727AspGlu: 4.727 ± 0.066	2.708AspPhe: 2.708 ± 0.052	3.515AspGly: 3.515 ± 0.079	0.939AspHis: 0.939 ± 0.031	4.06AspIle: 4.06 ± 0.062	2.994AspLys: 2.994 ± 0.063	4.867AspLeu: 4.867 ± 0.073	1.591AspMet: 1.591 ± 0.04	1.963AspAsn: 1.963 ± 0.046	1.593AspPro: 1.593 ± 0.039	1.594AspGln: 1.594 ± 0.04	2.02AspArg: 2.02 ± 0.044	2.463AspSer: 2.463 ± 0.053	3.003AspThr: 3.003 ± 0.06	4.451AspVal: 4.451 ± 0.076	0.544AspTrp: 0.544 ± 0.024	2.369AspTyr: 2.369 ± 0.047	0.0AspXaa: 0.0 ± 0.0
Glu	6.368GluAla: 6.368 ± 0.086	0.353GluCys: 0.353 ± 0.018	3.762GluAsp: 3.762 ± 0.074	6.177GluGlu: 6.177 ± 0.089	2.393GluPhe: 2.393 ± 0.047	3.953GluGly: 3.953 ± 0.065	1.657GluHis: 1.657 ± 0.039	4.682GluIle: 4.682 ± 0.074	5.657GluLys: 5.657 ± 0.076	6.974GluLeu: 6.974 ± 0.082	2.044GluMet: 2.044 ± 0.044	3.18GluAsn: 3.18 ± 0.054	2.006GluPro: 2.006 ± 0.042	4.239GluGln: 4.239 ± 0.078	3.692GluArg: 3.692 ± 0.077	2.863GluSer: 2.863 ± 0.064	3.715GluThr: 3.715 ± 0.058	4.931GluVal: 4.931 ± 0.076	0.809GluTrp: 0.809 ± 0.031	1.882GluTyr: 1.882 ± 0.051	0.0GluXaa: 0.0 ± 0.0
Phe	3.341PheAla: 3.341 ± 0.062	0.34PheCys: 0.34 ± 0.018	2.704PheAsp: 2.704 ± 0.055	3.191PheGlu: 3.191 ± 0.058	2.251PhePhe: 2.251 ± 0.055	3.108PheGly: 3.108 ± 0.056	0.943PheHis: 0.943 ± 0.029	3.613PheIle: 3.613 ± 0.072	2.435PheLys: 2.435 ± 0.05	4.153PheLeu: 4.153 ± 0.072	1.3PheMet: 1.3 ± 0.043	1.881PheAsn: 1.881 ± 0.041	1.444PhePro: 1.444 ± 0.037	1.416PheGln: 1.416 ± 0.044	1.417PheArg: 1.417 ± 0.035	2.842PheSer: 2.842 ± 0.053	2.886PheThr: 2.886 ± 0.06	3.298PheVal: 3.298 ± 0.068	0.405PheTrp: 0.405 ± 0.022	1.724PheTyr: 1.724 ± 0.042	0.001PheXaa: 0.001 ± 0.001
Gly	5.066GlyAla: 5.066 ± 0.096	0.479GlyCys: 0.479 ± 0.024	3.36GlyAsp: 3.36 ± 0.066	3.934GlyGlu: 3.934 ± 0.064	3.145GlyPhe: 3.145 ± 0.059	4.327GlyGly: 4.327 ± 0.089	1.466GlyHis: 1.466 ± 0.036	5.237GlyIle: 5.237 ± 0.08	4.363GlyLys: 4.363 ± 0.072	5.8GlyLeu: 5.8 ± 0.088	1.969GlyMet: 1.969 ± 0.049	2.332GlyAsn: 2.332 ± 0.052	1.527GlyPro: 1.527 ± 0.045	2.218GlyGln: 2.218 ± 0.047	2.52GlyArg: 2.52 ± 0.05	3.358GlySer: 3.358 ± 0.064	4.051GlyThr: 4.051 ± 0.073	4.967GlyVal: 4.967 ± 0.077	0.622GlyTrp: 0.622 ± 0.027	2.829GlyTyr: 2.829 ± 0.065	0.001GlyXaa: 0.001 ± 0.001
His	1.721HisAla: 1.721 ± 0.041	0.204HisCys: 0.204 ± 0.016	1.211HisAsp: 1.211 ± 0.036	1.533HisGlu: 1.533 ± 0.042	1.157HisPhe: 1.157 ± 0.038	1.326HisGly: 1.326 ± 0.037	0.672HisHis: 0.672 ± 0.029	1.735HisIle: 1.735 ± 0.045	1.077HisLys: 1.077 ± 0.035	2.136HisLeu: 2.136 ± 0.048	0.582HisMet: 0.582 ± 0.024	0.769HisAsn: 0.769 ± 0.024	1.05HisPro: 1.05 ± 0.032	0.904HisGln: 0.904 ± 0.033	0.874HisArg: 0.874 ± 0.028	1.02HisSer: 1.02 ± 0.037	1.216HisThr: 1.216 ± 0.034	1.616HisVal: 1.616 ± 0.038	0.196HisTrp: 0.196 ± 0.013	1.045HisTyr: 1.045 ± 0.034	0.0HisXaa: 0.0 ± 0.0
Ile	6.632IleAla: 6.632 ± 0.094	0.625IleCys: 0.625 ± 0.025	4.475IleAsp: 4.475 ± 0.071	5.446IleGlu: 5.446 ± 0.088	3.092IlePhe: 3.092 ± 0.069	5.389IleGly: 5.389 ± 0.085	1.609IleHis: 1.609 ± 0.04	5.473IleIle: 5.473 ± 0.097	3.565IleLys: 3.565 ± 0.061	6.568IleLeu: 6.568 ± 0.092	1.816IleMet: 1.816 ± 0.045	2.77IleAsn: 2.77 ± 0.057	2.963IlePro: 2.963 ± 0.058	2.822IleGln: 2.822 ± 0.059	2.899IleArg: 2.899 ± 0.059	4.146IleSer: 4.146 ± 0.066	4.268IleThr: 4.268 ± 0.065	5.982IleVal: 5.982 ± 0.086	0.594IleTrp: 0.594 ± 0.024	2.407IleTyr: 2.407 ± 0.049	0.0IleXaa: 0.0 ± 0.0
Lys	5.0LysAla: 5.0 ± 0.085	0.255LysCys: 0.255 ± 0.017	3.592LysAsp: 3.592 ± 0.074	5.688LysGlu: 5.688 ± 0.079	1.903LysPhe: 1.903 ± 0.041	3.881LysGly: 3.881 ± 0.065	1.29LysHis: 1.29 ± 0.036	4.21LysIle: 4.21 ± 0.067	5.781LysLys: 5.781 ± 0.101	5.635LysLeu: 5.635 ± 0.075	2.331LysMet: 2.331 ± 0.051	3.192LysAsn: 3.192 ± 0.064	2.153LysPro: 2.153 ± 0.051	3.356LysGln: 3.356 ± 0.072	3.114LysArg: 3.114 ± 0.052	3.189LysSer: 3.189 ± 0.057	3.84LysThr: 3.84 ± 0.067	4.219LysVal: 4.219 ± 0.078	0.747LysTrp: 0.747 ± 0.028	1.959LysTyr: 1.959 ± 0.048	0.0LysXaa: 0.0 ± 0.0
Leu	8.598LeuAla: 8.598 ± 0.107	0.671LeuCys: 0.671 ± 0.024	4.904LeuAsp: 4.904 ± 0.068	6.624LeuGlu: 6.624 ± 0.084	4.44LeuPhe: 4.44 ± 0.077	5.888LeuGly: 5.888 ± 0.079	2.171LeuHis: 2.171 ± 0.05	6.612LeuIle: 6.612 ± 0.119	6.221LeuLys: 6.221 ± 0.088	9.65LeuLeu: 9.65 ± 0.129	2.672LeuMet: 2.672 ± 0.057	3.977LeuAsn: 3.977 ± 0.066	3.837LeuPro: 3.837 ± 0.059	4.032LeuGln: 4.032 ± 0.069	3.797LeuArg: 3.797 ± 0.062	6.0LeuSer: 6.0 ± 0.077	6.05LeuThr: 6.05 ± 0.081	6.421LeuVal: 6.421 ± 0.097	0.733LeuTrp: 0.733 ± 0.032	3.349LeuTyr: 3.349 ± 0.066	0.0LeuXaa: 0.0 ± 0.0
Met	2.37MetAla: 2.37 ± 0.048	0.141MetCys: 0.141 ± 0.013	1.427MetAsp: 1.427 ± 0.036	1.702MetGlu: 1.702 ± 0.041	1.023MetPhe: 1.023 ± 0.034	1.589MetGly: 1.589 ± 0.038	0.62MetHis: 0.62 ± 0.024	2.055MetIle: 2.055 ± 0.044	2.439MetLys: 2.439 ± 0.046	2.953MetLeu: 2.953 ± 0.055	1.044MetMet: 1.044 ± 0.032	1.646MetAsn: 1.646 ± 0.042	1.156MetPro: 1.156 ± 0.035	1.17MetGln: 1.17 ± 0.034	1.25MetArg: 1.25 ± 0.04	1.705MetSer: 1.705 ± 0.039	2.179MetThr: 2.179 ± 0.046	1.633MetVal: 1.633 ± 0.04	0.218MetTrp: 0.218 ± 0.013	0.872MetTyr: 0.872 ± 0.032	0.001MetXaa: 0.001 ± 0.001
Asn	3.267AsnAla: 3.267 ± 0.062	0.264AsnCys: 0.264 ± 0.018	2.334AsnAsp: 2.334 ± 0.052	3.259AsnGlu: 3.259 ± 0.067	1.657AsnPhe: 1.657 ± 0.04	2.98AsnGly: 2.98 ± 0.056	0.975AsnHis: 0.975 ± 0.031	2.964AsnIle: 2.964 ± 0.067	2.462AsnLys: 2.462 ± 0.059	3.463AsnLeu: 3.463 ± 0.062	1.132AsnMet: 1.132 ± 0.029	1.848AsnAsn: 1.848 ± 0.057	1.864AsnPro: 1.864 ± 0.043	1.521AsnGln: 1.521 ± 0.04	1.831AsnArg: 1.831 ± 0.045	1.989AsnSer: 1.989 ± 0.056	2.332AsnThr: 2.332 ± 0.048	3.038AsnVal: 3.038 ± 0.056	0.446AsnTrp: 0.446 ± 0.021	1.632AsnTyr: 1.632 ± 0.041	0.0AsnXaa: 0.0 ± 0.0
Pro	2.57ProAla: 2.57 ± 0.053	0.156ProCys: 0.156 ± 0.015	1.761ProAsp: 1.761 ± 0.04	2.628ProGlu: 2.628 ± 0.047	1.84ProPhe: 1.84 ± 0.043	1.951ProGly: 1.951 ± 0.049	0.834ProHis: 0.834 ± 0.028	2.566ProIle: 2.566 ± 0.052	2.154ProLys: 2.154 ± 0.04	3.438ProLeu: 3.438 ± 0.059	0.926ProMet: 0.926 ± 0.032	1.454ProAsn: 1.454 ± 0.033	0.818ProPro: 0.818 ± 0.03	1.323ProGln: 1.323 ± 0.032	1.061ProArg: 1.061 ± 0.032	2.0ProSer: 2.0 ± 0.05	2.378ProThr: 2.378 ± 0.051	2.797ProVal: 2.797 ± 0.058	0.291ProTrp: 0.291 ± 0.014	1.44ProTyr: 1.44 ± 0.037	0.0ProXaa: 0.0 ± 0.0
Gln	3.548GlnAla: 3.548 ± 0.062	0.236GlnCys: 0.236 ± 0.017	1.684GlnAsp: 1.684 ± 0.043	2.585GlnGlu: 2.585 ± 0.054	1.859GlnPhe: 1.859 ± 0.043	2.18GlnGly: 2.18 ± 0.049	1.104GlnHis: 1.104 ± 0.032	2.503GlnIle: 2.503 ± 0.046	2.888GlnLys: 2.888 ± 0.057	4.938GlnLeu: 4.938 ± 0.086	1.204GlnMet: 1.204 ± 0.034	1.539GlnAsn: 1.539 ± 0.036	1.432GlnPro: 1.432 ± 0.039	3.235GlnGln: 3.235 ± 0.09	1.817GlnArg: 1.817 ± 0.044	2.023GlnSer: 2.023 ± 0.05	2.482GlnThr: 2.482 ± 0.049	2.534GlnVal: 2.534 ± 0.049	0.408GlnTrp: 0.408 ± 0.023	1.523GlnTyr: 1.523 ± 0.044	0.0GlnXaa: 0.0 ± 0.0
Arg	2.975ArgAla: 2.975 ± 0.056	0.236ArgCys: 0.236 ± 0.018	2.188ArgAsp: 2.188 ± 0.047	3.078ArgGlu: 3.078 ± 0.057	2.049ArgPhe: 2.049 ± 0.044	2.309ArgGly: 2.309 ± 0.047	0.967ArgHis: 0.967 ± 0.034	2.944ArgIle: 2.944 ± 0.063	2.721ArgLys: 2.721 ± 0.057	3.964ArgLeu: 3.964 ± 0.073	1.304ArgMet: 1.304 ± 0.037	1.652ArgAsn: 1.652 ± 0.041	1.275ArgPro: 1.275 ± 0.036	1.771ArgGln: 1.771 ± 0.049	1.746ArgArg: 1.746 ± 0.05	1.93ArgSer: 1.93 ± 0.044	2.198ArgThr: 2.198 ± 0.052	2.718ArgVal: 2.718 ± 0.05	0.325ArgTrp: 0.325 ± 0.018	1.777ArgTyr: 1.777 ± 0.038	0.0ArgXaa: 0.0 ± 0.0
Ser	3.947SerAla: 3.947 ± 0.066	0.395SerCys: 0.395 ± 0.022	2.755SerAsp: 2.755 ± 0.054	3.349SerGlu: 3.349 ± 0.062	2.828SerPhe: 2.828 ± 0.057	3.656SerGly: 3.656 ± 0.064	1.113SerHis: 1.113 ± 0.032	4.33SerIle: 4.33 ± 0.064	3.483SerLys: 3.483 ± 0.061	5.312SerLeu: 5.312 ± 0.077	1.658SerMet: 1.658 ± 0.046	2.257SerAsn: 2.257 ± 0.056	1.778SerPro: 1.778 ± 0.042	1.816SerGln: 1.816 ± 0.044	1.957SerArg: 1.957 ± 0.049	3.291SerSer: 3.291 ± 0.072	3.314SerThr: 3.314 ± 0.061	3.725SerVal: 3.725 ± 0.059	0.571SerTrp: 0.571 ± 0.024	2.247SerTyr: 2.247 ± 0.048	0.001SerXaa: 0.001 ± 0.001
Thr	5.218ThrAla: 5.218 ± 0.087	0.412ThrCys: 0.412 ± 0.021	2.921ThrAsp: 2.921 ± 0.051	3.61ThrGlu: 3.61 ± 0.06	2.83ThrPhe: 2.83 ± 0.065	3.978ThrGly: 3.978 ± 0.079	1.286ThrHis: 1.286 ± 0.037	4.917ThrIle: 4.917 ± 0.066	4.012ThrLys: 4.012 ± 0.068	5.981ThrLeu: 5.981 ± 0.071	1.629ThrMet: 1.629 ± 0.04	2.671ThrAsn: 2.671 ± 0.066	2.477ThrPro: 2.477 ± 0.05	2.077ThrGln: 2.077 ± 0.051	2.077ThrArg: 2.077 ± 0.051	3.382ThrSer: 3.382 ± 0.068	4.319ThrThr: 4.319 ± 0.087	4.689ThrVal: 4.689 ± 0.073	0.574ThrTrp: 0.574 ± 0.024	2.326ThrTyr: 2.326 ± 0.05	0.0ThrXaa: 0.0 ± 0.0
Val	6.365ValAla: 6.365 ± 0.093	0.598ValCys: 0.598 ± 0.026	3.9ValAsp: 3.9 ± 0.064	5.064ValGlu: 5.064 ± 0.072	3.05ValPhe: 3.05 ± 0.065	4.647ValGly: 4.647 ± 0.076	1.446ValHis: 1.446 ± 0.039	5.258ValIle: 5.258 ± 0.076	4.513ValLys: 4.513 ± 0.078	7.118ValLeu: 7.118 ± 0.087	2.018ValMet: 2.018 ± 0.045	2.706ValAsn: 2.706 ± 0.056	2.682ValPro: 2.682 ± 0.055	2.694ValGln: 2.694 ± 0.051	2.798ValArg: 2.798 ± 0.053	4.157ValSer: 4.157 ± 0.072	4.767ValThr: 4.767 ± 0.08	5.542ValVal: 5.542 ± 0.088	0.591ValTrp: 0.591 ± 0.024	2.407ValTyr: 2.407 ± 0.047	0.0ValXaa: 0.0 ± 0.0
Trp	0.555TrpAla: 0.555 ± 0.022	0.076TrpCys: 0.076 ± 0.011	0.487TrpAsp: 0.487 ± 0.024	0.438TrpGlu: 0.438 ± 0.022	0.456TrpPhe: 0.456 ± 0.021	0.534TrpGly: 0.534 ± 0.027	0.269TrpHis: 0.269 ± 0.016	0.686TrpIle: 0.686 ± 0.03	0.549TrpLys: 0.549 ± 0.025	1.203TrpLeu: 1.203 ± 0.038	0.308TrpMet: 0.308 ± 0.019	0.424TrpAsn: 0.424 ± 0.02	0.256TrpPro: 0.256 ± 0.016	0.535TrpGln: 0.535 ± 0.022	0.397TrpArg: 0.397 ± 0.02	0.528TrpSer: 0.528 ± 0.022	0.519TrpThr: 0.519 ± 0.026	0.556TrpVal: 0.556 ± 0.024	0.099TrpTrp: 0.099 ± 0.01	0.37TrpTyr: 0.37 ± 0.023	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.785TyrAla: 2.785 ± 0.051	0.3TyrCys: 0.3 ± 0.017	2.399TyrAsp: 2.399 ± 0.05	2.738TyrGlu: 2.738 ± 0.067	1.807TyrPhe: 1.807 ± 0.045	2.335TyrGly: 2.335 ± 0.052	0.776TyrHis: 0.776 ± 0.028	2.429TyrIle: 2.429 ± 0.052	2.302TyrLys: 2.302 ± 0.051	3.216TyrLeu: 3.216 ± 0.064	0.966TyrMet: 0.966 ± 0.029	1.647TyrAsn: 1.647 ± 0.047	1.321TyrPro: 1.321 ± 0.039	1.323TyrGln: 1.323 ± 0.034	1.592TyrArg: 1.592 ± 0.043	1.95TyrSer: 1.95 ± 0.042	2.346TyrThr: 2.346 ± 0.057	2.695TyrVal: 2.695 ± 0.048	0.346TyrTrp: 0.346 ± 0.019	1.506TyrTyr: 1.506 ± 0.046	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.002XaaPhe: 0.002 ± 0.002	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.001XaaPro: 0.001 ± 0.001	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.001XaaSer: 0.001 ± 0.001	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.001XaaTyr: 0.001 ± 0.001	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3621 proteins (1035375 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---