Chrysochloris asiatica (Cape golden mole)
Average proteome isoelectric point is 6.71
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 24928 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6I9JIQ6|A0A6I9JIQ6_CHRAS keratin-associated protein 24-1-like OS=Chrysochloris asiatica OX=185453 GN=LOC102822057 PE=4 SV=1
MM1 pKa = 7.97 DD2 pKa = 3.9 VLFVAILAVPLILGQKK18 pKa = 10.41 LEE20 pKa = 4.42 DD21 pKa = 3.8 EE22 pKa = 4.8 EE23 pKa = 4.62 EE24 pKa = 4.45 LEE26 pKa = 4.19 EE27 pKa = 5.58 DD28 pKa = 4.54 DD29 pKa = 4.69 YY30 pKa = 11.95 YY31 pKa = 11.39 QVIYY35 pKa = 10.03 EE36 pKa = 4.25 YY37 pKa = 10.41 TVTPHH42 pKa = 6.65 YY43 pKa = 11.15 DD44 pKa = 3.3 DD45 pKa = 4.87 FVVNFTIDD53 pKa = 3.25 YY54 pKa = 11.28 SMFEE58 pKa = 4.06 SEE60 pKa = 5.45 DD61 pKa = 3.63 RR62 pKa = 11.84 LVSLDD67 pKa = 3.88 KK68 pKa = 10.96 EE69 pKa = 4.34 VTEE72 pKa = 4.61 AAEE75 pKa = 4.2 TTTNLEE81 pKa = 4.29 TDD83 pKa = 3.73 STTHH87 pKa = 6.07 PVEE90 pKa = 3.86 LQKK93 pKa = 10.79 PVTMKK98 pKa = 10.51 PMTMEE103 pKa = 5.08 PSPDD107 pKa = 3.42 TNDD110 pKa = 3.18 AVSNLQSPVFLILSWALIQGGMYY133 pKa = 10.47 FMM135 pKa = 6.18
Molecular weight: 15.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.736
IPC2_protein 3.732
IPC_protein 3.681
Toseland 3.49
ProMoST 3.834
Dawson 3.668
Bjellqvist 3.821
Wikipedia 3.579
Rodwell 3.516
Grimsley 3.401
Solomon 3.643
Lehninger 3.605
Nozaki 3.783
DTASelect 3.948
Thurlkill 3.541
EMBOSS 3.592
Sillero 3.808
Patrickios 0.896
IPC_peptide 3.643
IPC2_peptide 3.783
IPC2.peptide.svr19 3.724
Protein with the highest isoelectric point:
>tr|A0A6I9KE09|A0A6I9KE09_CHRAS Neugrin OS=Chrysochloris asiatica OX=185453 GN=LOC102829756 PE=3 SV=1
MM1 pKa = 7.57 SSHH4 pKa = 5.15 KK5 pKa = 8.91 TFRR8 pKa = 11.84 IKK10 pKa = 10.64 RR11 pKa = 11.84 FLAKK15 pKa = 9.71 KK16 pKa = 9.58 QKK18 pKa = 8.69 QNRR21 pKa = 11.84 PIPQWIRR28 pKa = 11.84 MKK30 pKa = 9.89 TGNKK34 pKa = 8.66 IRR36 pKa = 11.84 YY37 pKa = 7.97 NSKK40 pKa = 8.63 KK41 pKa = 9.37 RR42 pKa = 11.84 HH43 pKa = 4.01 WRR45 pKa = 11.84 RR46 pKa = 11.84 TKK48 pKa = 10.77 LGLL51 pKa = 3.67
Molecular weight: 6.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.366
IPC2_protein 11.023
IPC_protein 12.325
Toseland 12.501
ProMoST 12.983
Dawson 12.501
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.398
Grimsley 12.544
Solomon 12.983
Lehninger 12.896
Nozaki 12.501
DTASelect 12.486
Thurlkill 12.501
EMBOSS 12.998
Sillero 12.501
Patrickios 12.135
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 8.966
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
19590
5338
24928
14419874
31
35420
578.5
64.54
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.762 ± 0.015
2.196 ± 0.013
4.841 ± 0.01
7.006 ± 0.02
3.722 ± 0.012
6.41 ± 0.023
2.589 ± 0.008
4.553 ± 0.016
5.776 ± 0.019
9.989 ± 0.021
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.248 ± 0.008
3.73 ± 0.011
6.117 ± 0.024
4.75 ± 0.015
5.528 ± 0.015
8.341 ± 0.02
5.438 ± 0.012
6.087 ± 0.014
1.172 ± 0.005
2.736 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here