Botryosphaeria dothidea victorivirus 1
Average proteome isoelectric point is 6.43
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A089H6F5|A0A089H6F5_9VIRU Coat protein OS=Botryosphaeria dothidea victorivirus 1 OX=1547580 GN=CP PE=4 SV=1
MM1 pKa = 7.23 EE2 pKa = 5.08 VEE4 pKa = 4.34 TQFRR8 pKa = 11.84 PSFLAGILLRR18 pKa = 11.84 PTGGRR23 pKa = 11.84 LAAEE27 pKa = 4.22 AGGRR31 pKa = 11.84 EE32 pKa = 4.27 YY33 pKa = 11.31 ASALNSTMTVRR44 pKa = 11.84 GITTTRR50 pKa = 11.84 SSVVRR55 pKa = 11.84 YY56 pKa = 7.26 HH57 pKa = 6.7 TSPTQGEE64 pKa = 4.41 LRR66 pKa = 11.84 DD67 pKa = 4.13 KK68 pKa = 11.28 LDD70 pKa = 4.21 LAPEE74 pKa = 4.58 GARR77 pKa = 11.84 RR78 pKa = 11.84 LVAEE82 pKa = 4.21 YY83 pKa = 8.23 QTSGEE88 pKa = 4.22 MVAHH92 pKa = 6.68 FSNLARR98 pKa = 11.84 KK99 pKa = 8.68 YY100 pKa = 10.88 VNFSANFEE108 pKa = 4.13 ASNLAGLVEE117 pKa = 5.09 RR118 pKa = 11.84 ISLGLSLAAWHH129 pKa = 7.18 DD130 pKa = 3.9 NVTAFSLRR138 pKa = 11.84 GGRR141 pKa = 11.84 EE142 pKa = 3.42 PDD144 pKa = 3.08 IAALGATTTPVNSVADD160 pKa = 3.66 CVFIPRR166 pKa = 11.84 VVNTVITGDD175 pKa = 3.26 IFSVLVAAATGCGAAVATDD194 pKa = 3.53 MLGVNDD200 pKa = 4.69 NGVALLNDD208 pKa = 2.96 VDD210 pKa = 4.01 ARR212 pKa = 11.84 RR213 pKa = 11.84 FPPAAVEE220 pKa = 3.92 ALRR223 pKa = 11.84 LLGANMNDD231 pKa = 3.42 AGQGALFALALTRR244 pKa = 11.84 GLHH247 pKa = 6.16 KK248 pKa = 10.41 GTVVQSTTRR257 pKa = 11.84 GGALTRR263 pKa = 11.84 AFLIAGTFSVPFGGINPTPEE283 pKa = 4.35 PYY285 pKa = 10.4 AALPALNSSDD295 pKa = 3.18 GVAVGGYY302 pKa = 10.25 VDD304 pKa = 4.68 GLCLATAAAVAHH316 pKa = 6.91 CDD318 pKa = 3.07 PGTTIEE324 pKa = 4.29 GLWYY328 pKa = 6.58 PTTYY332 pKa = 8.9 VTPRR336 pKa = 11.84 HH337 pKa = 6.13 DD338 pKa = 3.77 AFGVAGSVPTADD350 pKa = 4.15 PLNGASLWPQIISEE364 pKa = 4.21 SGRR367 pKa = 11.84 FLGEE371 pKa = 3.78 YY372 pKa = 10.13 VSALGRR378 pKa = 11.84 LFSAEE383 pKa = 4.48 DD384 pKa = 3.73 GLGLANRR391 pKa = 11.84 AACTMAHH398 pKa = 7.12 LALVDD403 pKa = 4.66 DD404 pKa = 5.18 WGCDD408 pKa = 3.55 DD409 pKa = 4.49 ATVAPYY415 pKa = 9.79 FWIEE419 pKa = 3.88 PTSILPQNWLGSAAEE434 pKa = 4.11 RR435 pKa = 11.84 SMSGALVTPGGEE447 pKa = 4.07 VTLPAVEE454 pKa = 4.62 GVQPVGDD461 pKa = 4.09 STGPWAGYY469 pKa = 8.54 VLEE472 pKa = 4.74 LSSARR477 pKa = 11.84 RR478 pKa = 11.84 NPLFIHH484 pKa = 6.84 FGGQMSEE491 pKa = 4.02 HH492 pKa = 7.06 LGTIVPRR499 pKa = 11.84 QLDD502 pKa = 4.0 VEE504 pKa = 4.79 TVVHH508 pKa = 6.12 PKK510 pKa = 10.16 LRR512 pKa = 11.84 AEE514 pKa = 3.99 QAALRR519 pKa = 11.84 DD520 pKa = 3.86 RR521 pKa = 11.84 LQASLGLDD529 pKa = 3.29 QYY531 pKa = 10.81 VHH533 pKa = 6.89 LPEE536 pKa = 5.11 RR537 pKa = 11.84 MPIPSPDD544 pKa = 3.14 NFMHH548 pKa = 6.05 VGCRR552 pKa = 11.84 MGVEE556 pKa = 4.5 VVHH559 pKa = 5.81 EE560 pKa = 3.98 QFVNYY565 pKa = 10.07 RR566 pKa = 11.84 MYY568 pKa = 11.2 PSMLPAAHH576 pKa = 6.85 EE577 pKa = 4.46 FAHH580 pKa = 5.6 STVTLSVTKK589 pKa = 10.3 PFGVPAGAAPEE600 pKa = 4.19 IEE602 pKa = 4.79 CGFSHH607 pKa = 7.17 EE608 pKa = 4.23 PTRR611 pKa = 11.84 TALMLSQTSHH621 pKa = 6.21 NLRR624 pKa = 11.84 GPSSVAFTGMLISTSAPAAPRR645 pKa = 11.84 YY646 pKa = 8.27 TSGQGRR652 pKa = 11.84 SRR654 pKa = 11.84 RR655 pKa = 11.84 VALGDD660 pKa = 3.32 EE661 pKa = 4.15 RR662 pKa = 11.84 HH663 pKa = 5.74 EE664 pKa = 4.35 VGSAGVDD671 pKa = 3.56 HH672 pKa = 6.86 NPSPPVPVGNRR683 pKa = 11.84 LGVTTHH689 pKa = 6.78 HH690 pKa = 7.13 DD691 pKa = 3.63 AGRR694 pKa = 11.84 GPAPIRR700 pKa = 11.84 RR701 pKa = 11.84 ADD703 pKa = 3.44 QVRR706 pKa = 11.84 GGGGGAPQRR715 pKa = 11.84 RR716 pKa = 11.84 RR717 pKa = 11.84 DD718 pKa = 3.78 DD719 pKa = 3.88 GQAQDD724 pKa = 4.45 PVAPPPAHH732 pKa = 6.94 GEE734 pKa = 3.9 PAPPEE739 pKa = 3.87 VDD741 pKa = 3.35 EE742 pKa = 4.52 PP743 pKa = 4.08
Molecular weight: 78.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.581
IPC2_protein 5.461
IPC_protein 5.512
Toseland 5.919
ProMoST 5.842
Dawson 5.741
Bjellqvist 5.766
Wikipedia 5.728
Rodwell 5.715
Grimsley 6.097
Solomon 5.741
Lehninger 5.728
Nozaki 5.995
DTASelect 6.173
Thurlkill 6.16
EMBOSS 6.122
Sillero 6.084
Patrickios 4.126
IPC_peptide 5.753
IPC2_peptide 6.109
IPC2.peptide.svr19 5.967
Protein with the highest isoelectric point:
>tr|A0A089H6F5|A0A089H6F5_9VIRU Coat protein OS=Botryosphaeria dothidea victorivirus 1 OX=1547580 GN=CP PE=4 SV=1
MM1 pKa = 7.17 QDD3 pKa = 2.92 AVAVRR8 pKa = 11.84 AAEE11 pKa = 3.98 VGPLGVEE18 pKa = 4.18 LLRR21 pKa = 11.84 VIDD24 pKa = 4.83 TFAAWTRR31 pKa = 11.84 THH33 pKa = 7.2 FPRR36 pKa = 11.84 EE37 pKa = 3.95 LVAGMSRR44 pKa = 11.84 LQVQRR49 pKa = 11.84 QQLDD53 pKa = 3.7 SIHH56 pKa = 7.05 PLLAPAIATLLLDD69 pKa = 3.71 YY70 pKa = 10.38 PLQLEE75 pKa = 4.37 LDD77 pKa = 3.85 HH78 pKa = 6.5 GVVMRR83 pKa = 11.84 LVDD86 pKa = 4.16 LALPRR91 pKa = 11.84 IDD93 pKa = 5.33 AEE95 pKa = 4.04 PAEE98 pKa = 4.25 PAKK101 pKa = 10.83 NQALRR106 pKa = 11.84 QHH108 pKa = 6.35 VKK110 pKa = 10.43 RR111 pKa = 11.84 NSPRR115 pKa = 11.84 HH116 pKa = 4.56 NQYY119 pKa = 9.6 MEE121 pKa = 3.97 LASAIRR127 pKa = 11.84 HH128 pKa = 5.7 DD129 pKa = 3.79 KK130 pKa = 10.4 PFRR133 pKa = 11.84 LACFPEE139 pKa = 4.76 KK140 pKa = 10.43 KK141 pKa = 10.18 LQAATAKK148 pKa = 10.53 KK149 pKa = 9.93 NFSTRR154 pKa = 11.84 RR155 pKa = 11.84 LLDD158 pKa = 3.48 SVSRR162 pKa = 11.84 EE163 pKa = 3.85 LGASFLGWLVAHH175 pKa = 6.79 CACKK179 pKa = 9.98 VTDD182 pKa = 4.72 DD183 pKa = 3.96 QFQMIIIFGLTLSTRR198 pKa = 11.84 LGRR201 pKa = 11.84 HH202 pKa = 5.71 AYY204 pKa = 10.05 LYY206 pKa = 10.43 ALSMVTNPSHH216 pKa = 6.85 AKK218 pKa = 9.6 SLNVVLKK225 pKa = 10.88 GLGANASTPGCWFVEE240 pKa = 4.5 GQGLLGRR247 pKa = 11.84 GVGDD251 pKa = 3.31 VDD253 pKa = 3.82 WNSEE257 pKa = 3.42 IPYY260 pKa = 10.13 RR261 pKa = 11.84 CDD263 pKa = 3.31 PDD265 pKa = 3.71 LVRR268 pKa = 11.84 EE269 pKa = 4.05 KK270 pKa = 10.05 TINVDD275 pKa = 3.22 PEE277 pKa = 4.29 TIRR280 pKa = 11.84 PHH282 pKa = 5.72 IRR284 pKa = 11.84 AILEE288 pKa = 4.01 RR289 pKa = 11.84 EE290 pKa = 4.01 LVNRR294 pKa = 11.84 NDD296 pKa = 3.94 LPDD299 pKa = 4.47 MEE301 pKa = 5.82 DD302 pKa = 2.98 FWTSRR307 pKa = 11.84 WLWCVNGSQTSDD319 pKa = 2.83 SDD321 pKa = 3.61 KK322 pKa = 11.6 ALGLDD327 pKa = 3.92 LKK329 pKa = 9.88 TLQTHH334 pKa = 5.07 RR335 pKa = 11.84 RR336 pKa = 11.84 RR337 pKa = 11.84 YY338 pKa = 9.75 RR339 pKa = 11.84 RR340 pKa = 11.84 MAAEE344 pKa = 4.16 ALVDD348 pKa = 4.0 NPIPDD353 pKa = 3.79 WDD355 pKa = 3.53 GTTYY359 pKa = 10.89 VSASSKK365 pKa = 11.11 LEE367 pKa = 3.85 VGKK370 pKa = 8.55 TRR372 pKa = 11.84 AIFACDD378 pKa = 3.1 TRR380 pKa = 11.84 SYY382 pKa = 11.04 FAFSYY387 pKa = 10.91 LLNQVQKK394 pKa = 9.94 DD395 pKa = 3.44 WRR397 pKa = 11.84 NEE399 pKa = 3.78 RR400 pKa = 11.84 VLLDD404 pKa = 4.04 PGNGGTVAMGARR416 pKa = 11.84 LRR418 pKa = 11.84 NAQKK422 pKa = 10.89 GGGFNLMLDD431 pKa = 3.58 YY432 pKa = 11.47 DD433 pKa = 4.75 DD434 pKa = 6.31 FNSQHH439 pKa = 5.4 STEE442 pKa = 3.96 TMKK445 pKa = 10.63 IVFEE449 pKa = 4.43 EE450 pKa = 4.25 TCKK453 pKa = 10.39 IYY455 pKa = 10.56 NAPAWYY461 pKa = 8.68 TDD463 pKa = 3.74 KK464 pKa = 11.22 LCSSFDD470 pKa = 3.18 KK471 pKa = 10.65 MYY473 pKa = 10.49 IRR475 pKa = 11.84 QKK477 pKa = 10.92 DD478 pKa = 3.84 GLRR481 pKa = 11.84 HH482 pKa = 4.58 VAGTLMSGHH491 pKa = 7.15 RR492 pKa = 11.84 GTTFINSVLNAAYY505 pKa = 9.78 LRR507 pKa = 11.84 CGVGSGWFDD516 pKa = 3.12 HH517 pKa = 6.82 ALSLHH522 pKa = 6.26 TGDD525 pKa = 5.04 DD526 pKa = 3.7 VYY528 pKa = 11.08 IRR530 pKa = 11.84 SNSRR534 pKa = 11.84 SEE536 pKa = 3.82 VSNILTRR543 pKa = 11.84 AAEE546 pKa = 4.58 FGCRR550 pKa = 11.84 MNPTKK555 pKa = 10.6 QSIGVKK561 pKa = 8.6 NCEE564 pKa = 4.22 FLRR567 pKa = 11.84 CAYY570 pKa = 10.18 NPYY573 pKa = 9.91 YY574 pKa = 10.63 AVGYY578 pKa = 9.26 LCRR581 pKa = 11.84 TVGTLVNGNWSGDD594 pKa = 3.66 TPLTPHH600 pKa = 6.86 EE601 pKa = 4.92 ALTSLLTSLRR611 pKa = 11.84 SLYY614 pKa = 10.3 NRR616 pKa = 11.84 SLGGGLGRR624 pKa = 11.84 YY625 pKa = 7.71 LASAIRR631 pKa = 11.84 FRR633 pKa = 11.84 TDD635 pKa = 3.71 GISRR639 pKa = 11.84 KK640 pKa = 9.28 NLIGLLDD647 pKa = 3.55 GRR649 pKa = 11.84 IAIEE653 pKa = 4.82 GGPCFNADD661 pKa = 2.99 AKK663 pKa = 10.83 IRR665 pKa = 11.84 TCALKK670 pKa = 9.19 NTIKK674 pKa = 10.63 PSPLVDD680 pKa = 4.2 KK681 pKa = 11.19 NWDD684 pKa = 3.14 AYY686 pKa = 9.38 ATRR689 pKa = 11.84 DD690 pKa = 3.65 YY691 pKa = 10.82 LVCHH695 pKa = 5.61 TTDD698 pKa = 3.21 LEE700 pKa = 4.55 YY701 pKa = 11.12 YY702 pKa = 10.25 ALQNCGVSPTPLMVTTSYY720 pKa = 11.41 EE721 pKa = 3.68 KK722 pKa = 10.71 GLNRR726 pKa = 11.84 EE727 pKa = 4.14 SEE729 pKa = 4.28 EE730 pKa = 4.0 VKK732 pKa = 10.42 PLKK735 pKa = 9.77 FCNMRR740 pKa = 11.84 VSTARR745 pKa = 11.84 GFATVSEE752 pKa = 4.18 LLKK755 pKa = 10.59 VDD757 pKa = 3.6 TEE759 pKa = 4.71 GGVLAHH765 pKa = 6.48 YY766 pKa = 8.56 PLLMLIKK773 pKa = 10.5 DD774 pKa = 3.97 SLSPSLIRR782 pKa = 11.84 EE783 pKa = 4.07 LCEE786 pKa = 3.84 ADD788 pKa = 4.67 GYY790 pKa = 9.29 TPPSEE795 pKa = 4.53 NIRR798 pKa = 11.84 AYY800 pKa = 10.65 AFGEE804 pKa = 4.01 DD805 pKa = 3.86 FRR807 pKa = 11.84 THH809 pKa = 6.64 NIIGRR814 pKa = 11.84 LPYY817 pKa = 10.3 SDD819 pKa = 4.93 AAALQGKK826 pKa = 6.27 TSCDD830 pKa = 3.59 NIVADD835 pKa = 3.82 YY836 pKa = 10.71 KK837 pKa = 10.88 IYY839 pKa = 10.97 LL840 pKa = 3.86
Molecular weight: 93.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.277
IPC2_protein 7.614
IPC_protein 7.395
Toseland 7.029
ProMoST 8.009
Dawson 8.112
Bjellqvist 8.565
Wikipedia 8.024
Rodwell 8.126
Grimsley 7.088
Solomon 8.141
Lehninger 8.156
Nozaki 8.756
DTASelect 8.244
Thurlkill 8.302
EMBOSS 8.317
Sillero 8.639
Patrickios 4.228
IPC_peptide 8.141
IPC2_peptide 7.658
IPC2.peptide.svr19 7.628
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1583
743
840
791.5
85.93
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.36 ± 1.48
1.832 ± 0.518
5.433 ± 0.588
4.738 ± 0.166
3.285 ± 0.055
8.718 ± 1.314
2.653 ± 0.211
3.538 ± 0.58
2.59 ± 1.315
10.36 ± 0.921
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.021 ± 0.002
4.043 ± 0.558
6.001 ± 1.238
2.716 ± 0.017
7.138 ± 0.281
6.57 ± 0.017
6.57 ± 0.202
7.202 ± 0.969
1.263 ± 0.22
2.969 ± 0.652
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here