Amino acid dipepetide frequency for bacterium HR35

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.04AlaAla: 2.04 ± 0.134
0.354AlaCys: 0.354 ± 0.045
1.969AlaAsp: 1.969 ± 0.103
3.264AlaGlu: 3.264 ± 0.135
2.714AlaPhe: 2.714 ± 0.119
2.856AlaGly: 2.856 ± 0.14
0.588AlaHis: 0.588 ± 0.054
3.917AlaIle: 3.917 ± 0.148
4.657AlaLys: 4.657 ± 0.168
6.076AlaLeu: 6.076 ± 0.202
0.528AlaMet: 0.528 ± 0.053
2.252AlaAsn: 2.252 ± 0.107
1.344AlaPro: 1.344 ± 0.106
1.768AlaGln: 1.768 ± 0.119
1.763AlaArg: 1.763 ± 0.106
2.758AlaSer: 2.758 ± 0.13
1.899AlaThr: 1.899 ± 0.125
2.345AlaVal: 2.345 ± 0.128
0.43AlaTrp: 0.43 ± 0.047
2.035AlaTyr: 2.035 ± 0.1
0.0AlaXaa: 0.0 ± 0.0
Cys
0.234CysAla: 0.234 ± 0.038
0.082CysCys: 0.082 ± 0.021
0.294CysAsp: 0.294 ± 0.051
0.392CysGlu: 0.392 ± 0.053
0.468CysPhe: 0.468 ± 0.056
0.653CysGly: 0.653 ± 0.067
0.114CysHis: 0.114 ± 0.022
0.261CysIle: 0.261 ± 0.037
0.511CysLys: 0.511 ± 0.058
0.794CysLeu: 0.794 ± 0.071
0.027CysMet: 0.027 ± 0.012
0.359CysAsn: 0.359 ± 0.062
0.462CysPro: 0.462 ± 0.057
0.321CysGln: 0.321 ± 0.05
0.316CysArg: 0.316 ± 0.044
0.566CysSer: 0.566 ± 0.074
0.288CysThr: 0.288 ± 0.041
0.245CysVal: 0.245 ± 0.037
0.109CysTrp: 0.109 ± 0.026
0.37CysTyr: 0.37 ± 0.095
0.0CysXaa: 0.0 ± 0.0
Asp
1.567AspAla: 1.567 ± 0.101
0.403AspCys: 0.403 ± 0.054
1.572AspAsp: 1.572 ± 0.106
3.427AspGlu: 3.427 ± 0.155
3.884AspPhe: 3.884 ± 0.159
2.187AspGly: 2.187 ± 0.133
0.566AspHis: 0.566 ± 0.049
3.525AspIle: 3.525 ± 0.134
4.161AspLys: 4.161 ± 0.152
5.783AspLeu: 5.783 ± 0.186
0.49AspMet: 0.49 ± 0.053
1.92AspAsn: 1.92 ± 0.108
2.013AspPro: 2.013 ± 0.114
0.707AspGln: 0.707 ± 0.059
1.262AspArg: 1.262 ± 0.081
1.953AspSer: 1.953 ± 0.093
1.137AspThr: 1.137 ± 0.089
2.23AspVal: 2.23 ± 0.112
0.658AspTrp: 0.658 ± 0.062
2.535AspTyr: 2.535 ± 0.134
0.0AspXaa: 0.0 ± 0.0
Glu
4.036GluAla: 4.036 ± 0.177
0.375GluCys: 0.375 ± 0.054
3.155GluAsp: 3.155 ± 0.147
9.09GluGlu: 9.09 ± 0.331
3.868GluPhe: 3.868 ± 0.16
3.938GluGly: 3.938 ± 0.177
0.62GluHis: 0.62 ± 0.062
11.19GluIle: 11.19 ± 0.307
11.543GluLys: 11.543 ± 0.316
6.947GluLeu: 6.947 ± 0.193
1.148GluMet: 1.148 ± 0.084
5.505GluAsn: 5.505 ± 0.203
1.909GluPro: 1.909 ± 0.113
1.066GluGln: 1.066 ± 0.071
3.362GluArg: 3.362 ± 0.162
2.774GluSer: 2.774 ± 0.14
3.585GluThr: 3.585 ± 0.127
4.744GluVal: 4.744 ± 0.181
0.582GluTrp: 0.582 ± 0.065
2.301GluTyr: 2.301 ± 0.11
0.0GluXaa: 0.0 ± 0.0
Phe
2.617PheAla: 2.617 ± 0.135
0.528PheCys: 0.528 ± 0.066
2.899PheAsp: 2.899 ± 0.128
4.782PheGlu: 4.782 ± 0.179
4.711PhePhe: 4.711 ± 0.254
2.899PheGly: 2.899 ± 0.121
0.713PheHis: 0.713 ± 0.072
5.173PheIle: 5.173 ± 0.191
6.011PheLys: 6.011 ± 0.218
9.645PheLeu: 9.645 ± 0.395
0.734PheMet: 0.734 ± 0.064
3.607PheAsn: 3.607 ± 0.143
2.225PhePro: 2.225 ± 0.12
1.899PheGln: 1.899 ± 0.099
2.274PheArg: 2.274 ± 0.116
4.967PheSer: 4.967 ± 0.226
2.018PheThr: 2.018 ± 0.107
3.204PheVal: 3.204 ± 0.144
0.93PheTrp: 0.93 ± 0.072
3.237PheTyr: 3.237 ± 0.153
0.0PheXaa: 0.0 ± 0.0
Gly
2.513GlyAla: 2.513 ± 0.115
0.408GlyCys: 0.408 ± 0.049
2.317GlyAsp: 2.317 ± 0.113
4.521GlyGlu: 4.521 ± 0.166
3.433GlyPhe: 3.433 ± 0.159
3.89GlyGly: 3.89 ± 0.21
0.832GlyHis: 0.832 ± 0.063
5.179GlyIle: 5.179 ± 0.198
5.233GlyLys: 5.233 ± 0.178
5.467GlyLeu: 5.467 ± 0.174
0.718GlyMet: 0.718 ± 0.057
2.546GlyAsn: 2.546 ± 0.149
1.567GlyPro: 1.567 ± 0.109
1.273GlyGln: 1.273 ± 0.087
2.317GlyArg: 2.317 ± 0.128
3.204GlySer: 3.204 ± 0.196
2.443GlyThr: 2.443 ± 0.15
3.405GlyVal: 3.405 ± 0.148
0.74GlyTrp: 0.74 ± 0.088
2.758GlyTyr: 2.758 ± 0.125
0.0GlyXaa: 0.0 ± 0.0
His
0.332HisAla: 0.332 ± 0.042
0.071HisCys: 0.071 ± 0.017
0.348HisAsp: 0.348 ± 0.042
0.549HisGlu: 0.549 ± 0.065
0.919HisPhe: 0.919 ± 0.091
0.642HisGly: 0.642 ± 0.058
0.326HisHis: 0.326 ± 0.042
0.734HisIle: 0.734 ± 0.076
0.87HisLys: 0.87 ± 0.074
1.893HisLeu: 1.893 ± 0.104
0.163HisMet: 0.163 ± 0.027
0.626HisAsn: 0.626 ± 0.058
0.93HisPro: 0.93 ± 0.087
0.566HisGln: 0.566 ± 0.053
0.696HisArg: 0.696 ± 0.068
0.642HisSer: 0.642 ± 0.063
0.544HisThr: 0.544 ± 0.048
0.332HisVal: 0.332 ± 0.04
0.125HisTrp: 0.125 ± 0.024
0.588HisTyr: 0.588 ± 0.052
0.0HisXaa: 0.0 ± 0.0
Ile
4.406IleAla: 4.406 ± 0.151
0.745IleCys: 0.745 ± 0.084
4.651IleAsp: 4.651 ± 0.164
7.235IleGlu: 7.235 ± 0.227
7.328IlePhe: 7.328 ± 0.29
4.711IleGly: 4.711 ± 0.193
1.006IleHis: 1.006 ± 0.073
8.726IleIle: 8.726 ± 0.253
10.575IleLys: 10.575 ± 0.273
11.766IleLeu: 11.766 ± 0.373
1.023IleMet: 1.023 ± 0.072
5.462IleAsn: 5.462 ± 0.192
3.28IlePro: 3.28 ± 0.15
2.279IleGln: 2.279 ± 0.119
3.34IleArg: 3.34 ± 0.144
5.766IleSer: 5.766 ± 0.194
3.862IleThr: 3.862 ± 0.172
4.689IleVal: 4.689 ± 0.205
0.609IleTrp: 0.609 ± 0.054
4.243IleTyr: 4.243 ± 0.172
0.0IleXaa: 0.0 ± 0.0
Lys
4.292LysAla: 4.292 ± 0.164
0.555LysCys: 0.555 ± 0.055
4.564LysAsp: 4.564 ± 0.197
11.75LysGlu: 11.75 ± 0.332
5.184LysPhe: 5.184 ± 0.219
4.461LysGly: 4.461 ± 0.161
0.947LysHis: 0.947 ± 0.07
14.345LysIle: 14.345 ± 0.348
12.196LysLys: 12.196 ± 0.378
9.46LysLeu: 9.46 ± 0.27
1.741LysMet: 1.741 ± 0.083
8.122LysAsn: 8.122 ± 0.291
3.226LysPro: 3.226 ± 0.145
2.263LysGln: 2.263 ± 0.133
3.971LysArg: 3.971 ± 0.151
4.047LysSer: 4.047 ± 0.16
4.776LysThr: 4.776 ± 0.182
5.304LysVal: 5.304 ± 0.203
0.68LysTrp: 0.68 ± 0.078
3.808LysTyr: 3.808 ± 0.16
0.0LysXaa: 0.0 ± 0.0
Leu
7.017LeuAla: 7.017 ± 0.213
0.5LeuCys: 0.5 ± 0.048
4.602LeuAsp: 4.602 ± 0.174
10.015LeuGlu: 10.015 ± 0.299
5.864LeuPhe: 5.864 ± 0.23
6.609LeuGly: 6.609 ± 0.216
1.306LeuHis: 1.306 ± 0.097
11.696LeuIle: 11.696 ± 0.315
14.051LeuLys: 14.051 ± 0.368
10.194LeuLeu: 10.194 ± 0.292
1.463LeuMet: 1.463 ± 0.083
7.224LeuAsn: 7.224 ± 0.208
4.896LeuPro: 4.896 ± 0.201
2.78LeuGln: 2.78 ± 0.13
4.684LeuArg: 4.684 ± 0.156
6.626LeuSer: 6.626 ± 0.235
5.173LeuThr: 5.173 ± 0.184
6.49LeuVal: 6.49 ± 0.229
0.838LeuTrp: 0.838 ± 0.064
3.275LeuTyr: 3.275 ± 0.142
0.0LeuXaa: 0.0 ± 0.0
Met
0.724MetAla: 0.724 ± 0.063
0.049MetCys: 0.049 ± 0.017
0.522MetAsp: 0.522 ± 0.048
0.93MetGlu: 0.93 ± 0.071
0.62MetPhe: 0.62 ± 0.068
0.642MetGly: 0.642 ± 0.067
0.103MetHis: 0.103 ± 0.026
1.289MetIle: 1.289 ± 0.071
1.871MetLys: 1.871 ± 0.094
0.963MetLeu: 0.963 ± 0.074
0.305MetMet: 0.305 ± 0.05
0.68MetAsn: 0.68 ± 0.061
0.549MetPro: 0.549 ± 0.048
0.277MetGln: 0.277 ± 0.035
0.56MetArg: 0.56 ± 0.06
0.68MetSer: 0.68 ± 0.063
0.533MetThr: 0.533 ± 0.056
0.702MetVal: 0.702 ± 0.066
0.092MetTrp: 0.092 ± 0.024
0.234MetTyr: 0.234 ± 0.032
0.0MetXaa: 0.0 ± 0.0
Asn
1.572AsnAla: 1.572 ± 0.116
0.5AsnCys: 0.5 ± 0.058
2.078AsnAsp: 2.078 ± 0.124
3.443AsnGlu: 3.443 ± 0.142
5.32AsnPhe: 5.32 ± 0.228
2.432AsnGly: 2.432 ± 0.125
0.696AsnHis: 0.696 ± 0.058
4.385AsnIle: 4.385 ± 0.182
6.452AsnLys: 6.452 ± 0.212
9.275AsnLeu: 9.275 ± 0.295
0.555AsnMet: 0.555 ± 0.05
3.579AsnAsn: 3.579 ± 0.205
2.91AsnPro: 2.91 ± 0.162
2.214AsnGln: 2.214 ± 0.127
1.681AsnArg: 1.681 ± 0.099
3.139AsnSer: 3.139 ± 0.173
1.512AsnThr: 1.512 ± 0.104
2.198AsnVal: 2.198 ± 0.126
0.658AsnTrp: 0.658 ± 0.06
3.324AsnTyr: 3.324 ± 0.142
0.0AsnXaa: 0.0 ± 0.0
Pro
1.681ProAla: 1.681 ± 0.107
0.261ProCys: 0.261 ± 0.046
1.692ProAsp: 1.692 ± 0.096
3.579ProGlu: 3.579 ± 0.156
2.268ProPhe: 2.268 ± 0.124
2.187ProGly: 2.187 ± 0.107
0.642ProHis: 0.642 ± 0.049
2.698ProIle: 2.698 ± 0.118
3.079ProLys: 3.079 ± 0.137
4.189ProLeu: 4.189 ± 0.161
0.294ProMet: 0.294 ± 0.045
1.953ProAsn: 1.953 ± 0.111
1.768ProPro: 1.768 ± 0.126
1.964ProGln: 1.964 ± 0.093
1.458ProArg: 1.458 ± 0.096
2.301ProSer: 2.301 ± 0.131
1.953ProThr: 1.953 ± 0.141
1.915ProVal: 1.915 ± 0.122
0.354ProTrp: 0.354 ± 0.046
1.795ProTyr: 1.795 ± 0.113
0.0ProXaa: 0.0 ± 0.0
Gln
1.79GlnAla: 1.79 ± 0.118
0.098GlnCys: 0.098 ± 0.022
1.159GlnAsp: 1.159 ± 0.086
2.785GlnGlu: 2.785 ± 0.116
1.093GlnPhe: 1.093 ± 0.072
1.627GlnGly: 1.627 ± 0.093
0.256GlnHis: 0.256 ± 0.038
2.785GlnIle: 2.785 ± 0.133
3.558GlnLys: 3.558 ± 0.149
2.181GlnLeu: 2.181 ± 0.109
0.413GlnMet: 0.413 ± 0.049
2.083GlnAsn: 2.083 ± 0.13
1.175GlnPro: 1.175 ± 0.092
0.702GlnGln: 0.702 ± 0.057
1.458GlnArg: 1.458 ± 0.093
1.295GlnSer: 1.295 ± 0.077
1.616GlnThr: 1.616 ± 0.104
1.616GlnVal: 1.616 ± 0.111
0.19GlnTrp: 0.19 ± 0.032
0.653GlnTyr: 0.653 ± 0.059
0.0GlnXaa: 0.0 ± 0.0
Arg
1.708ArgAla: 1.708 ± 0.107
0.294ArgCys: 0.294 ± 0.05
1.779ArgAsp: 1.779 ± 0.109
3.286ArgGlu: 3.286 ± 0.151
2.394ArgPhe: 2.394 ± 0.131
2.383ArgGly: 2.383 ± 0.126
0.522ArgHis: 0.522 ± 0.063
3.868ArgIle: 3.868 ± 0.163
4.134ArgLys: 4.134 ± 0.15
3.71ArgLeu: 3.71 ± 0.155
0.571ArgMet: 0.571 ± 0.054
2.16ArgAsn: 2.16 ± 0.12
1.202ArgPro: 1.202 ± 0.091
1.072ArgGln: 1.072 ± 0.071
1.871ArgArg: 1.871 ± 0.132
1.545ArgSer: 1.545 ± 0.093
1.539ArgThr: 1.539 ± 0.103
2.41ArgVal: 2.41 ± 0.119
0.375ArgTrp: 0.375 ± 0.053
1.431ArgTyr: 1.431 ± 0.086
0.0ArgXaa: 0.0 ± 0.0
Ser
2.475SerAla: 2.475 ± 0.113
0.506SerCys: 0.506 ± 0.07
2.138SerAsp: 2.138 ± 0.129
3.509SerGlu: 3.509 ± 0.136
4.433SerPhe: 4.433 ± 0.176
3.318SerGly: 3.318 ± 0.211
0.62SerHis: 0.62 ± 0.063
4.004SerIle: 4.004 ± 0.172
4.493SerLys: 4.493 ± 0.176
7.915SerLeu: 7.915 ± 0.234
0.598SerMet: 0.598 ± 0.051
2.502SerAsn: 2.502 ± 0.161
2.377SerPro: 2.377 ± 0.123
2.296SerGln: 2.296 ± 0.13
1.937SerArg: 1.937 ± 0.101
4.053SerSer: 4.053 ± 0.225
2.66SerThr: 2.66 ± 0.2
2.589SerVal: 2.589 ± 0.137
0.577SerTrp: 0.577 ± 0.065
2.573SerTyr: 2.573 ± 0.139
0.0SerXaa: 0.0 ± 0.0
Thr
1.964ThrAla: 1.964 ± 0.119
0.337ThrCys: 0.337 ± 0.045
1.714ThrAsp: 1.714 ± 0.099
2.481ThrGlu: 2.481 ± 0.129
2.219ThrPhe: 2.219 ± 0.125
2.943ThrGly: 2.943 ± 0.138
0.598ThrHis: 0.598 ± 0.058
3.732ThrIle: 3.732 ± 0.146
3.52ThrLys: 3.52 ± 0.157
4.58ThrLeu: 4.58 ± 0.165
0.413ThrMet: 0.413 ± 0.049
2.127ThrAsn: 2.127 ± 0.134
2.366ThrPro: 2.366 ± 0.163
1.403ThrGln: 1.403 ± 0.093
1.469ThrArg: 1.469 ± 0.092
3.193ThrSer: 3.193 ± 0.232
2.508ThrThr: 2.508 ± 0.2
1.795ThrVal: 1.795 ± 0.118
0.359ThrTrp: 0.359 ± 0.052
1.877ThrTyr: 1.877 ± 0.116
0.0ThrXaa: 0.0 ± 0.0
Val
2.513ValAla: 2.513 ± 0.149
0.408ValCys: 0.408 ± 0.058
2.345ValAsp: 2.345 ± 0.12
3.248ValGlu: 3.248 ± 0.144
3.688ValPhe: 3.688 ± 0.176
3.046ValGly: 3.046 ± 0.152
0.609ValHis: 0.609 ± 0.052
5.113ValIle: 5.113 ± 0.19
4.885ValLys: 4.885 ± 0.182
6.332ValLeu: 6.332 ± 0.184
0.653ValMet: 0.653 ± 0.068
2.807ValAsn: 2.807 ± 0.143
1.811ValPro: 1.811 ± 0.108
1.126ValGln: 1.126 ± 0.105
1.773ValArg: 1.773 ± 0.119
3.155ValSer: 3.155 ± 0.143
1.942ValThr: 1.942 ± 0.142
3.014ValVal: 3.014 ± 0.152
0.462ValTrp: 0.462 ± 0.05
2.595ValTyr: 2.595 ± 0.103
0.0ValXaa: 0.0 ± 0.0
Trp
0.441TrpAla: 0.441 ± 0.044
0.092TrpCys: 0.092 ± 0.025
0.332TrpAsp: 0.332 ± 0.043
0.762TrpGlu: 0.762 ± 0.059
0.653TrpPhe: 0.653 ± 0.06
0.702TrpGly: 0.702 ± 0.064
0.114TrpHis: 0.114 ± 0.029
0.68TrpIle: 0.68 ± 0.068
0.604TrpLys: 0.604 ± 0.062
1.235TrpLeu: 1.235 ± 0.074
0.141TrpMet: 0.141 ± 0.025
0.397TrpAsn: 0.397 ± 0.048
0.239TrpPro: 0.239 ± 0.035
0.664TrpGln: 0.664 ± 0.061
0.495TrpArg: 0.495 ± 0.056
0.511TrpSer: 0.511 ± 0.059
0.343TrpThr: 0.343 ± 0.046
0.408TrpVal: 0.408 ± 0.045
0.196TrpTrp: 0.196 ± 0.036
0.424TrpTyr: 0.424 ± 0.069
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.632TyrAla: 1.632 ± 0.09
0.299TyrCys: 0.299 ± 0.043
1.85TyrAsp: 1.85 ± 0.129
2.693TyrGlu: 2.693 ± 0.134
4.009TyrPhe: 4.009 ± 0.172
2.638TyrGly: 2.638 ± 0.171
0.718TyrHis: 0.718 ± 0.063
2.404TyrIle: 2.404 ± 0.125
3.291TyrLys: 3.291 ± 0.15
6.898TyrLeu: 6.898 ± 0.236
0.343TyrMet: 0.343 ± 0.039
2.013TyrAsn: 2.013 ± 0.12
1.779TyrPro: 1.779 ± 0.107
1.893TyrGln: 1.893 ± 0.098
1.567TyrArg: 1.567 ± 0.093
2.399TyrSer: 2.399 ± 0.118
1.267TyrThr: 1.267 ± 0.125
1.893TyrVal: 1.893 ± 0.115
0.468TyrTrp: 0.468 ± 0.049
2.181TyrTyr: 2.181 ± 0.114
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 609 proteins (183829 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski