Phytophthora kernoviae
Average proteome isoelectric point is 6.14
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9833 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A421ETC8|A0A421ETC8_9STRA CULLIN_2 domain-containing protein OS=Phytophthora kernoviae OX=325452 GN=BBI17_004979 PE=3 SV=1
MM1 pKa = 7.18 HH2 pKa = 6.79 TTFSLALVAAAVVLVDD18 pKa = 4.85 AKK20 pKa = 8.71 PTYY23 pKa = 9.44 VARR26 pKa = 11.84 IPNGANVPDD35 pKa = 4.21 VAALGHH41 pKa = 6.17 VDD43 pKa = 3.63 PAGGGANNDD52 pKa = 3.26 FGLDD56 pKa = 3.61 FEE58 pKa = 5.08 SAGEE62 pKa = 4.01 SWTTEE67 pKa = 4.0 FCQKK71 pKa = 10.97 DD72 pKa = 3.47 SDD74 pKa = 5.26 GDD76 pKa = 3.91 GQTNGQEE83 pKa = 4.74 LGDD86 pKa = 4.45 PCCQWVQDD94 pKa = 4.14 SNAVLEE100 pKa = 4.4 WTTGVSHH107 pKa = 7.42 PGDD110 pKa = 3.31 ASKK113 pKa = 9.84 TSDD116 pKa = 3.15 EE117 pKa = 4.57 SLWASITCGSSNSTTASSTAGSSSSTGTTDD147 pKa = 3.43 AGTPTTTDD155 pKa = 2.6 TDD157 pKa = 3.67 ATTSSSGGATASSSSAASAVTPGVYY182 pKa = 9.99 SAVGVVAVVATFFLL196 pKa = 4.51
Molecular weight: 19.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.703
IPC2_protein 3.719
IPC_protein 3.706
Toseland 3.49
ProMoST 3.897
Dawson 3.719
Bjellqvist 3.872
Wikipedia 3.694
Rodwell 3.541
Grimsley 3.401
Solomon 3.706
Lehninger 3.656
Nozaki 3.846
DTASelect 4.101
Thurlkill 3.554
EMBOSS 3.694
Sillero 3.834
Patrickios 0.896
IPC_peptide 3.694
IPC2_peptide 3.808
IPC2.peptide.svr19 3.771
Protein with the highest isoelectric point:
>tr|A0A3R7JS05|A0A3R7JS05_9STRA Uncharacterized protein OS=Phytophthora kernoviae OX=325452 GN=BBI17_004821 PE=4 SV=1
MM1 pKa = 7.62 ALFRR5 pKa = 11.84 SLTRR9 pKa = 11.84 QFKK12 pKa = 9.74 ALSVAAPRR20 pKa = 11.84 VAAQAPRR27 pKa = 11.84 FAAVASVPSTTPASLVTTLALRR49 pKa = 11.84 SAINFPQLQTRR60 pKa = 11.84 SMGYY64 pKa = 9.78 KK65 pKa = 10.2 LKK67 pKa = 10.17 TKK69 pKa = 10.65 ASVKK73 pKa = 10.09 KK74 pKa = 10.36 RR75 pKa = 11.84 FRR77 pKa = 11.84 VNCNGVVKK85 pKa = 10.65 RR86 pKa = 11.84 SQANKK91 pKa = 9.53 RR92 pKa = 11.84 HH93 pKa = 5.93 IATKK97 pKa = 8.75 KK98 pKa = 7.02 TRR100 pKa = 11.84 EE101 pKa = 3.84 RR102 pKa = 11.84 IRR104 pKa = 11.84 RR105 pKa = 11.84 LGKK108 pKa = 9.37 PVFVQGKK115 pKa = 7.01 IRR117 pKa = 11.84 KK118 pKa = 9.17 NILRR122 pKa = 11.84 MLAKK126 pKa = 10.4
Molecular weight: 14.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.359
IPC2_protein 10.877
IPC_protein 12.325
Toseland 12.501
ProMoST 12.983
Dawson 12.501
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.31
Grimsley 12.53
Solomon 12.983
Lehninger 12.881
Nozaki 12.501
DTASelect 12.486
Thurlkill 12.501
EMBOSS 12.983
Sillero 12.501
Patrickios 12.032
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.034
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9833
0
9833
4605710
49
5336
468.4
52.04
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.715 ± 0.026
1.527 ± 0.011
5.806 ± 0.016
6.868 ± 0.03
3.969 ± 0.016
6.331 ± 0.024
2.29 ± 0.012
4.44 ± 0.016
5.379 ± 0.026
9.455 ± 0.027
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.654 ± 0.01
3.729 ± 0.012
4.382 ± 0.016
4.17 ± 0.017
5.594 ± 0.019
7.612 ± 0.026
5.838 ± 0.02
7.301 ± 0.019
1.161 ± 0.008
2.779 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here