Bacillus phage DK1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Salasmaviridae; Northropvirinae; Hemphillvirus; Bacillus virus DK1

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3T0IIV1|A0A3T0IIV1_9CAUD Uncharacterized protein OS=Bacillus phage DK1 OX=2500808 GN=DK1_000016 PE=4 SV=1
MM1 pKa = 7.74AIVTKK6 pKa = 10.04TLTFEE11 pKa = 4.38GKK13 pKa = 9.14RR14 pKa = 11.84AGSIVEE20 pKa = 4.37NPNIAYY26 pKa = 10.59LNDD29 pKa = 3.33VEE31 pKa = 4.76LTQTQYY37 pKa = 11.55DD38 pKa = 3.57QLYY41 pKa = 9.11GSEE44 pKa = 4.13LRR46 pKa = 11.84VTEE49 pKa = 3.91TDD51 pKa = 3.28VLKK54 pKa = 10.93FKK56 pKa = 10.7FVIPNDD62 pKa = 4.28AILGYY67 pKa = 9.81SEE69 pKa = 5.63GDD71 pKa = 2.79IFYY74 pKa = 10.23KK75 pKa = 10.54SKK77 pKa = 10.11MVSGEE82 pKa = 3.89NEE84 pKa = 3.94LRR86 pKa = 11.84CKK88 pKa = 10.77DD89 pKa = 3.3KK90 pKa = 11.2AVYY93 pKa = 10.62NDD95 pKa = 3.44DD96 pKa = 3.34TSTYY100 pKa = 9.03KK101 pKa = 10.46QKK103 pKa = 11.12LLATYY108 pKa = 10.33QIDD111 pKa = 4.04LDD113 pKa = 4.04NTNEE117 pKa = 4.01LNFNITTTGEE127 pKa = 3.78YY128 pKa = 10.38GIYY131 pKa = 9.03EE132 pKa = 4.29VKK134 pKa = 10.69VDD136 pKa = 5.23VIYY139 pKa = 11.16NDD141 pKa = 3.58NGLNSYY147 pKa = 9.57GGQYY151 pKa = 10.4QYY153 pKa = 11.58DD154 pKa = 3.58QGSGVIVTVPIQNKK168 pKa = 9.03KK169 pKa = 10.36LEE171 pKa = 4.61EE172 pKa = 4.1IVTEE176 pKa = 4.29LEE178 pKa = 3.94GRR180 pKa = 11.84PTNSITNVTFAQIGVEE196 pKa = 4.3SVDD199 pKa = 3.25

Molecular weight: 22.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3T0IIU3|A0A3T0IIU3_9CAUD DNA-directed DNA polymerase OS=Bacillus phage DK1 OX=2500808 GN=DK1_000017 PE=4 SV=1
MM1 pKa = 7.67NINKK5 pKa = 8.24VFPLEE10 pKa = 3.9NKK12 pKa = 9.32FRR14 pKa = 11.84LRR16 pKa = 11.84KK17 pKa = 10.1GKK19 pKa = 8.59MLYY22 pKa = 9.98PRR24 pKa = 11.84TKK26 pKa = 10.2PLFKK30 pKa = 10.22RR31 pKa = 11.84YY32 pKa = 9.19LQIHH36 pKa = 4.98YY37 pKa = 10.06LIRR40 pKa = 11.84KK41 pKa = 8.3NSNPSKK47 pKa = 10.9

Molecular weight: 5.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

7594

38

776

155.0

17.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.029 ± 0.439

1.067 ± 0.164

6.558 ± 0.29

7.835 ± 0.569

4.398 ± 0.258

6.084 ± 0.458

1.83 ± 0.153

7.295 ± 0.374

9.376 ± 0.581

7.203 ± 0.384

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.437 ± 0.25

6.769 ± 0.408

2.634 ± 0.248

3.542 ± 0.185

4.359 ± 0.27

4.925 ± 0.162

6.005 ± 0.56

6.426 ± 0.398

1.29 ± 0.175

4.938 ± 0.249
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski