Amino acid dipepetide frequency for Bacillus phage DK1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.922AlaAla: 0.922 ± 0.419
0.659AlaCys: 0.659 ± 0.27
2.897AlaAsp: 2.897 ± 0.936
3.293AlaGlu: 3.293 ± 0.795
2.897AlaPhe: 2.897 ± 0.586
2.897AlaGly: 2.897 ± 0.699
0.395AlaHis: 0.395 ± 0.265
2.502AlaIle: 2.502 ± 0.542
4.61AlaLys: 4.61 ± 0.982
2.371AlaLeu: 2.371 ± 0.548
1.317AlaMet: 1.317 ± 0.346
2.107AlaAsn: 2.107 ± 0.458
1.317AlaPro: 1.317 ± 0.597
1.449AlaGln: 1.449 ± 0.467
1.317AlaArg: 1.317 ± 0.317
1.449AlaSer: 1.449 ± 0.467
2.766AlaThr: 2.766 ± 0.67
2.502AlaVal: 2.502 ± 0.693
0.395AlaTrp: 0.395 ± 0.214
2.239AlaTyr: 2.239 ± 0.504
0.0AlaXaa: 0.0 ± 0.0
Cys
0.527CysAla: 0.527 ± 0.296
0.395CysCys: 0.395 ± 0.343
1.054CysAsp: 1.054 ± 0.443
1.185CysGlu: 1.185 ± 0.48
0.263CysPhe: 0.263 ± 0.192
1.185CysGly: 1.185 ± 0.501
0.132CysHis: 0.132 ± 0.177
0.395CysIle: 0.395 ± 0.325
1.976CysLys: 1.976 ± 0.52
0.263CysLeu: 0.263 ± 0.174
0.395CysMet: 0.395 ± 0.311
0.263CysAsn: 0.263 ± 0.164
0.395CysPro: 0.395 ± 0.179
0.132CysGln: 0.132 ± 0.135
0.263CysArg: 0.263 ± 0.207
0.132CysSer: 0.132 ± 0.13
0.659CysThr: 0.659 ± 0.266
0.527CysVal: 0.527 ± 0.324
0.132CysTrp: 0.132 ± 0.13
0.395CysTyr: 0.395 ± 0.198
0.0CysXaa: 0.0 ± 0.0
Asp
2.897AspAla: 2.897 ± 0.566
0.527AspCys: 0.527 ± 0.221
4.214AspAsp: 4.214 ± 0.855
4.478AspGlu: 4.478 ± 0.592
3.819AspPhe: 3.819 ± 0.581
3.688AspGly: 3.688 ± 0.793
0.79AspHis: 0.79 ± 0.278
5.005AspIle: 5.005 ± 1.046
6.453AspLys: 6.453 ± 1.098
4.741AspLeu: 4.741 ± 0.797
2.634AspMet: 2.634 ± 0.569
3.688AspAsn: 3.688 ± 0.656
2.766AspPro: 2.766 ± 0.712
2.766AspGln: 2.766 ± 0.567
2.239AspArg: 2.239 ± 0.49
3.293AspSer: 3.293 ± 0.679
3.293AspThr: 3.293 ± 1.0
5.663AspVal: 5.663 ± 0.856
0.527AspTrp: 0.527 ± 0.287
2.107AspTyr: 2.107 ± 0.551
0.0AspXaa: 0.0 ± 0.0
Glu
3.293GluAla: 3.293 ± 0.733
0.79GluCys: 0.79 ± 0.357
3.819GluAsp: 3.819 ± 0.631
6.058GluGlu: 6.058 ± 1.526
4.478GluPhe: 4.478 ± 0.928
4.61GluGly: 4.61 ± 0.932
1.185GluHis: 1.185 ± 0.351
6.585GluIle: 6.585 ± 1.099
8.165GluLys: 8.165 ± 1.917
8.034GluLeu: 8.034 ± 1.305
2.107GluMet: 2.107 ± 0.525
6.322GluAsn: 6.322 ± 1.088
1.449GluPro: 1.449 ± 0.469
2.239GluGln: 2.239 ± 0.519
3.293GluArg: 3.293 ± 0.893
3.688GluSer: 3.688 ± 0.76
2.766GluThr: 2.766 ± 0.704
5.4GluVal: 5.4 ± 1.089
0.922GluTrp: 0.922 ± 0.315
3.161GluTyr: 3.161 ± 0.655
0.0GluXaa: 0.0 ± 0.0
Phe
1.317PheAla: 1.317 ± 0.388
0.79PheCys: 0.79 ± 0.295
3.424PheAsp: 3.424 ± 0.642
3.161PheGlu: 3.161 ± 0.519
1.185PhePhe: 1.185 ± 0.31
1.844PheGly: 1.844 ± 0.567
1.054PheHis: 1.054 ± 0.445
4.083PheIle: 4.083 ± 0.653
5.136PheLys: 5.136 ± 1.183
2.766PheLeu: 2.766 ± 0.675
1.58PheMet: 1.58 ± 0.576
3.029PheAsn: 3.029 ± 0.691
1.185PhePro: 1.185 ± 0.343
1.449PheGln: 1.449 ± 0.367
1.054PheArg: 1.054 ± 0.43
2.239PheSer: 2.239 ± 0.482
2.634PheThr: 2.634 ± 0.478
3.029PheVal: 3.029 ± 0.602
0.263PheTrp: 0.263 ± 0.181
2.766PheTyr: 2.766 ± 0.612
0.0PheXaa: 0.0 ± 0.0
Gly
3.029GlyAla: 3.029 ± 0.747
0.263GlyCys: 0.263 ± 0.182
2.897GlyAsp: 2.897 ± 0.884
5.005GlyGlu: 5.005 ± 0.598
2.634GlyPhe: 2.634 ± 0.579
3.029GlyGly: 3.029 ± 0.685
0.922GlyHis: 0.922 ± 0.353
4.873GlyIle: 4.873 ± 0.624
5.927GlyLys: 5.927 ± 1.414
4.741GlyLeu: 4.741 ± 0.693
2.107GlyMet: 2.107 ± 0.585
4.083GlyAsn: 4.083 ± 0.776
0.263GlyPro: 0.263 ± 0.183
2.239GlyGln: 2.239 ± 0.645
2.766GlyArg: 2.766 ± 0.631
3.951GlySer: 3.951 ± 0.526
3.951GlyThr: 3.951 ± 0.776
3.688GlyVal: 3.688 ± 0.935
0.79GlyTrp: 0.79 ± 0.306
3.688GlyTyr: 3.688 ± 0.751
0.0GlyXaa: 0.0 ± 0.0
His
0.527HisAla: 0.527 ± 0.267
0.527HisCys: 0.527 ± 0.223
0.79HisAsp: 0.79 ± 0.317
1.054HisGlu: 1.054 ± 0.449
0.659HisPhe: 0.659 ± 0.247
0.79HisGly: 0.79 ± 0.398
0.395HisHis: 0.395 ± 0.221
1.976HisIle: 1.976 ± 0.427
0.659HisLys: 0.659 ± 0.299
2.239HisLeu: 2.239 ± 0.595
0.527HisMet: 0.527 ± 0.305
1.317HisAsn: 1.317 ± 0.476
0.132HisPro: 0.132 ± 0.12
0.79HisGln: 0.79 ± 0.317
1.317HisArg: 1.317 ± 0.359
1.054HisSer: 1.054 ± 0.371
1.185HisThr: 1.185 ± 0.44
0.79HisVal: 0.79 ± 0.286
0.0HisTrp: 0.0 ± 0.0
1.58HisTyr: 1.58 ± 0.465
0.0HisXaa: 0.0 ± 0.0
Ile
2.897IleAla: 2.897 ± 0.678
0.79IleCys: 0.79 ± 0.334
5.795IleAsp: 5.795 ± 0.93
5.927IleGlu: 5.927 ± 1.033
2.107IlePhe: 2.107 ± 0.495
4.214IleGly: 4.214 ± 0.618
1.844IleHis: 1.844 ± 0.419
5.268IleIle: 5.268 ± 1.213
6.717IleLys: 6.717 ± 0.95
3.556IleLeu: 3.556 ± 0.737
1.976IleMet: 1.976 ± 0.473
5.268IleAsn: 5.268 ± 0.832
1.712IlePro: 1.712 ± 0.358
2.766IleGln: 2.766 ± 0.46
3.161IleArg: 3.161 ± 0.504
3.951IleSer: 3.951 ± 0.729
4.741IleThr: 4.741 ± 0.81
5.795IleVal: 5.795 ± 1.003
1.054IleTrp: 1.054 ± 0.348
3.424IleTyr: 3.424 ± 0.742
0.0IleXaa: 0.0 ± 0.0
Lys
3.293LysAla: 3.293 ± 0.855
1.185LysCys: 1.185 ± 0.436
5.927LysAsp: 5.927 ± 0.858
9.482LysGlu: 9.482 ± 2.078
4.083LysPhe: 4.083 ± 0.704
4.741LysGly: 4.741 ± 0.771
2.239LysHis: 2.239 ± 0.522
5.136LysIle: 5.136 ± 0.916
9.351LysLys: 9.351 ± 1.196
5.927LysLeu: 5.927 ± 0.845
5.005LysMet: 5.005 ± 1.079
5.268LysAsn: 5.268 ± 0.948
2.239LysPro: 2.239 ± 0.658
2.766LysGln: 2.766 ± 0.664
4.741LysArg: 4.741 ± 0.815
4.083LysSer: 4.083 ± 0.681
6.19LysThr: 6.19 ± 0.937
6.717LysVal: 6.717 ± 0.694
1.185LysTrp: 1.185 ± 0.302
4.214LysTyr: 4.214 ± 0.994
0.0LysXaa: 0.0 ± 0.0
Leu
2.634LeuAla: 2.634 ± 0.602
0.527LeuCys: 0.527 ± 0.289
6.058LeuAsp: 6.058 ± 0.856
5.4LeuGlu: 5.4 ± 0.909
2.107LeuPhe: 2.107 ± 0.525
4.346LeuGly: 4.346 ± 0.676
2.371LeuHis: 2.371 ± 0.645
4.61LeuIle: 4.61 ± 0.701
6.98LeuLys: 6.98 ± 1.018
3.819LeuLeu: 3.819 ± 0.695
2.897LeuMet: 2.897 ± 0.558
5.005LeuAsn: 5.005 ± 0.702
1.976LeuPro: 1.976 ± 0.482
3.688LeuGln: 3.688 ± 0.744
4.61LeuArg: 4.61 ± 0.791
3.688LeuSer: 3.688 ± 0.675
4.214LeuThr: 4.214 ± 0.724
3.424LeuVal: 3.424 ± 0.63
1.054LeuTrp: 1.054 ± 0.396
2.502LeuTyr: 2.502 ± 0.567
0.0LeuXaa: 0.0 ± 0.0
Met
1.185MetAla: 1.185 ± 0.319
0.263MetCys: 0.263 ± 0.17
2.239MetAsp: 2.239 ± 0.546
1.976MetGlu: 1.976 ± 0.663
1.712MetPhe: 1.712 ± 0.521
2.239MetGly: 2.239 ± 0.472
0.132MetHis: 0.132 ± 0.117
2.239MetIle: 2.239 ± 0.621
3.029MetLys: 3.029 ± 0.621
3.424MetLeu: 3.424 ± 0.701
1.449MetMet: 1.449 ± 0.49
3.688MetAsn: 3.688 ± 0.761
0.79MetPro: 0.79 ± 0.266
0.79MetGln: 0.79 ± 0.297
1.58MetArg: 1.58 ± 0.436
1.58MetSer: 1.58 ± 0.456
1.844MetThr: 1.844 ± 0.652
1.844MetVal: 1.844 ± 0.501
0.79MetTrp: 0.79 ± 0.335
1.58MetTyr: 1.58 ± 0.524
0.0MetXaa: 0.0 ± 0.0
Asn
1.976AsnAla: 1.976 ± 0.736
0.395AsnCys: 0.395 ± 0.251
3.819AsnAsp: 3.819 ± 0.768
5.268AsnGlu: 5.268 ± 0.657
2.897AsnPhe: 2.897 ± 0.568
3.951AsnGly: 3.951 ± 0.796
0.922AsnHis: 0.922 ± 0.323
5.005AsnIle: 5.005 ± 0.945
7.112AsnLys: 7.112 ± 1.441
5.136AsnLeu: 5.136 ± 0.879
1.58AsnMet: 1.58 ± 0.488
4.346AsnAsn: 4.346 ± 0.657
2.502AsnPro: 2.502 ± 0.606
2.502AsnGln: 2.502 ± 0.617
3.424AsnArg: 3.424 ± 0.776
4.478AsnSer: 4.478 ± 0.744
3.688AsnThr: 3.688 ± 0.747
3.688AsnVal: 3.688 ± 0.662
1.185AsnTrp: 1.185 ± 0.312
3.819AsnTyr: 3.819 ± 0.684
0.0AsnXaa: 0.0 ± 0.0
Pro
0.922ProAla: 0.922 ± 0.333
0.263ProCys: 0.263 ± 0.196
1.185ProAsp: 1.185 ± 0.419
2.107ProGlu: 2.107 ± 0.55
1.58ProPhe: 1.58 ± 0.4
1.054ProGly: 1.054 ± 0.415
0.395ProHis: 0.395 ± 0.195
1.976ProIle: 1.976 ± 0.529
2.107ProLys: 2.107 ± 0.452
1.58ProLeu: 1.58 ± 0.512
0.922ProMet: 0.922 ± 0.318
1.712ProAsn: 1.712 ± 0.535
1.185ProPro: 1.185 ± 0.306
0.922ProGln: 0.922 ± 0.306
1.185ProArg: 1.185 ± 0.367
2.107ProSer: 2.107 ± 0.589
2.107ProThr: 2.107 ± 0.501
1.054ProVal: 1.054 ± 0.413
0.263ProTrp: 0.263 ± 0.178
1.712ProTyr: 1.712 ± 0.491
0.0ProXaa: 0.0 ± 0.0
Gln
1.58GlnAla: 1.58 ± 0.45
0.0GlnCys: 0.0 ± 0.0
1.712GlnAsp: 1.712 ± 0.464
1.976GlnGlu: 1.976 ± 0.58
1.054GlnPhe: 1.054 ± 0.38
2.239GlnGly: 2.239 ± 0.774
0.395GlnHis: 0.395 ± 0.215
2.371GlnIle: 2.371 ± 0.525
3.951GlnLys: 3.951 ± 0.857
3.556GlnLeu: 3.556 ± 0.63
0.79GlnMet: 0.79 ± 0.266
2.107GlnAsn: 2.107 ± 0.549
1.185GlnPro: 1.185 ± 0.381
1.58GlnGln: 1.58 ± 0.437
2.239GlnArg: 2.239 ± 0.631
1.185GlnSer: 1.185 ± 0.39
2.634GlnThr: 2.634 ± 0.495
1.844GlnVal: 1.844 ± 0.534
0.395GlnTrp: 0.395 ± 0.234
2.634GlnTyr: 2.634 ± 0.588
0.0GlnXaa: 0.0 ± 0.0
Arg
2.766ArgAla: 2.766 ± 0.608
0.79ArgCys: 0.79 ± 0.321
2.634ArgAsp: 2.634 ± 0.614
4.478ArgGlu: 4.478 ± 0.816
1.976ArgPhe: 1.976 ± 0.544
2.239ArgGly: 2.239 ± 0.749
0.395ArgHis: 0.395 ± 0.228
2.502ArgIle: 2.502 ± 0.608
4.346ArgLys: 4.346 ± 0.847
2.634ArgLeu: 2.634 ± 0.558
1.976ArgMet: 1.976 ± 0.622
2.897ArgAsn: 2.897 ± 0.614
0.922ArgPro: 0.922 ± 0.307
2.371ArgGln: 2.371 ± 0.53
1.976ArgArg: 1.976 ± 0.518
1.054ArgSer: 1.054 ± 0.329
2.371ArgThr: 2.371 ± 0.535
1.712ArgVal: 1.712 ± 0.45
0.659ArgTrp: 0.659 ± 0.25
2.897ArgTyr: 2.897 ± 0.553
0.0ArgXaa: 0.0 ± 0.0
Ser
2.371SerAla: 2.371 ± 0.468
0.79SerCys: 0.79 ± 0.266
3.161SerAsp: 3.161 ± 0.745
3.819SerGlu: 3.819 ± 0.695
2.371SerPhe: 2.371 ± 0.742
3.424SerGly: 3.424 ± 0.595
1.054SerHis: 1.054 ± 0.377
4.083SerIle: 4.083 ± 0.676
2.766SerLys: 2.766 ± 0.597
4.214SerLeu: 4.214 ± 0.735
1.58SerMet: 1.58 ± 0.36
3.951SerAsn: 3.951 ± 0.603
0.922SerPro: 0.922 ± 0.273
1.844SerGln: 1.844 ± 0.581
1.58SerArg: 1.58 ± 0.485
1.844SerSer: 1.844 ± 0.598
2.502SerThr: 2.502 ± 0.738
3.688SerVal: 3.688 ± 0.601
0.395SerTrp: 0.395 ± 0.187
2.897SerTyr: 2.897 ± 0.511
0.0SerXaa: 0.0 ± 0.0
Thr
2.766ThrAla: 2.766 ± 1.265
0.659ThrCys: 0.659 ± 0.274
4.214ThrAsp: 4.214 ± 0.963
3.819ThrGlu: 3.819 ± 0.774
2.371ThrPhe: 2.371 ± 0.551
4.873ThrGly: 4.873 ± 0.885
0.79ThrHis: 0.79 ± 0.39
4.873ThrIle: 4.873 ± 0.78
4.741ThrLys: 4.741 ± 0.654
4.61ThrLeu: 4.61 ± 0.826
1.712ThrMet: 1.712 ± 0.427
3.556ThrAsn: 3.556 ± 0.847
0.922ThrPro: 0.922 ± 0.308
2.107ThrGln: 2.107 ± 0.631
2.239ThrArg: 2.239 ± 0.627
3.424ThrSer: 3.424 ± 0.675
4.214ThrThr: 4.214 ± 1.12
4.873ThrVal: 4.873 ± 0.722
0.527ThrTrp: 0.527 ± 0.236
2.766ThrTyr: 2.766 ± 0.576
0.0ThrXaa: 0.0 ± 0.0
Val
2.371ValAla: 2.371 ± 0.619
0.527ValCys: 0.527 ± 0.274
5.927ValAsp: 5.927 ± 0.678
5.268ValGlu: 5.268 ± 0.877
2.634ValPhe: 2.634 ± 0.497
5.4ValGly: 5.4 ± 0.611
1.449ValHis: 1.449 ± 0.464
5.005ValIle: 5.005 ± 0.929
5.268ValLys: 5.268 ± 0.668
3.951ValLeu: 3.951 ± 0.582
1.185ValMet: 1.185 ± 0.407
4.083ValAsn: 4.083 ± 0.73
2.502ValPro: 2.502 ± 0.502
1.58ValGln: 1.58 ± 0.463
2.107ValArg: 2.107 ± 0.459
2.897ValSer: 2.897 ± 0.525
4.873ValThr: 4.873 ± 1.239
4.083ValVal: 4.083 ± 1.045
1.317ValTrp: 1.317 ± 0.409
1.844ValTyr: 1.844 ± 0.56
0.0ValXaa: 0.0 ± 0.0
Trp
0.395TrpAla: 0.395 ± 0.212
0.0TrpCys: 0.0 ± 0.0
0.659TrpAsp: 0.659 ± 0.292
1.185TrpGlu: 1.185 ± 0.469
1.449TrpPhe: 1.449 ± 0.33
0.659TrpGly: 0.659 ± 0.328
0.527TrpHis: 0.527 ± 0.193
0.659TrpIle: 0.659 ± 0.283
1.054TrpLys: 1.054 ± 0.304
1.185TrpLeu: 1.185 ± 0.427
0.659TrpMet: 0.659 ± 0.202
1.054TrpAsn: 1.054 ± 0.388
0.0TrpPro: 0.0 ± 0.0
0.395TrpGln: 0.395 ± 0.219
0.527TrpArg: 0.527 ± 0.273
0.263TrpSer: 0.263 ± 0.16
0.395TrpThr: 0.395 ± 0.23
0.922TrpVal: 0.922 ± 0.29
0.132TrpTrp: 0.132 ± 0.127
0.79TrpTyr: 0.79 ± 0.3
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.634TyrAla: 2.634 ± 0.542
0.527TyrCys: 0.527 ± 0.325
3.161TyrAsp: 3.161 ± 0.631
3.424TyrGlu: 3.424 ± 0.521
1.712TyrPhe: 1.712 ± 0.487
3.424TyrGly: 3.424 ± 0.623
0.922TyrHis: 0.922 ± 0.34
3.819TyrIle: 3.819 ± 0.823
3.424TyrLys: 3.424 ± 0.674
3.293TyrLeu: 3.293 ± 0.662
1.844TyrMet: 1.844 ± 0.56
3.951TyrAsn: 3.951 ± 0.47
1.976TyrPro: 1.976 ± 0.426
1.054TyrGln: 1.054 ± 0.443
1.976TyrArg: 1.976 ± 0.502
2.897TyrSer: 2.897 ± 0.805
3.029TyrThr: 3.029 ± 0.521
3.029TyrVal: 3.029 ± 0.645
0.922TyrTrp: 0.922 ± 0.343
2.371TyrTyr: 2.371 ± 0.481
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 49 proteins (7594 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski