Brevilactibacter flavus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Propionibacteriaceae; Brevilactibacter

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2639 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q2EGH3|A0A4Q2EGH3_9ACTN Lysine--tRNA ligase OS=Brevilactibacter flavus OX=2072026 GN=lysS PE=3 SV=1
MM1 pKa = 7.6VDD3 pKa = 5.35LSIKK7 pKa = 10.01DD8 pKa = 3.68ANATGGGCGCGCGGHH23 pKa = 6.78DD24 pKa = 5.08AKK26 pKa = 11.15ADD28 pKa = 3.58QAAPASQGAVVADD41 pKa = 3.7YY42 pKa = 9.48TVTGMTCGHH51 pKa = 6.52CVAAVTEE58 pKa = 4.46EE59 pKa = 4.19VSAIPGVTDD68 pKa = 3.08VTVDD72 pKa = 3.98LEE74 pKa = 4.39SGALKK79 pKa = 10.53VVSDD83 pKa = 4.43DD84 pKa = 4.45AVDD87 pKa = 4.23FDD89 pKa = 5.62RR90 pKa = 11.84IVEE93 pKa = 4.23AVAEE97 pKa = 4.42AGDD100 pKa = 3.81YY101 pKa = 9.35TVAA104 pKa = 4.43

Molecular weight: 10.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q2EJX6|A0A4Q2EJX6_9ACTN tRNA (adenine(58)-N(1))-methyltransferase TrmI OS=Brevilactibacter flavus OX=2072026 GN=C1706_01730 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84SRR15 pKa = 11.84THH17 pKa = 6.18GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84SILAARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 10.2GRR40 pKa = 11.84VRR42 pKa = 11.84LAGG45 pKa = 3.53

Molecular weight: 5.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2639

0

2639

881072

29

2006

333.9

35.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.474 ± 0.074

0.666 ± 0.014

5.916 ± 0.039

5.269 ± 0.05

2.99 ± 0.028

8.876 ± 0.039

2.158 ± 0.025

4.158 ± 0.036

2.036 ± 0.036

10.38 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.989 ± 0.019

2.001 ± 0.027

5.657 ± 0.036

3.045 ± 0.024

7.19 ± 0.054

5.529 ± 0.032

6.195 ± 0.04

8.926 ± 0.051

1.595 ± 0.022

1.95 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski